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Removal of Contaminating TEM-la β-Lactamase Gene from Removal of Contaminating TEM-la β-Lactamase Gene from
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HOME > J. Microbiol > Volume 44(1); 2006 > Article
Research Support, Non-U.S. Gov't
Removal of Contaminating TEM-la β-Lactamase Gene from Removal of Contaminating TEM-la β-Lactamase Gene from
Jae Seok Song 1, Jung Hun Lee 1,2, Jung-Hyun Lee 2, Byeong Chul Jeong 1, Won-Keun Lee 1, Sang Hee Lee 1
Journal of Microbiology 2006;44(1):126-128
DOI: https://doi.org/2326 [pii]
1Department of Biological Sciences, Myongji University, San 38-2 Namdong, Yongin, Gyeonggido, 449-728, Republic of Korea, 2Marine Biotechnology Center, Korea Ocean Research & Development Institute, Ansan P.O. Box 29, Seoul 425-600,Republic of Korea1Department of Biological Sciences, Myongji University, San 38-2 Namdong, Yongin, Gyeonggido, 449-728, Republic of Korea, 2Marine Biotechnology Center, Korea Ocean Research & Development Institute, Ansan P.O. Box 29, Seoul 425-600,Republic of Korea
Corresponding author:  Sang Hee Lee , Tel: 82-31-330-6195, 
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Abstract

This study confirms that Taq DNA polymerase could be contaminated with the blaTEM-1a gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.

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    Removal of Contaminating TEM-la β-Lactamase Gene from Removal of Contaminating TEM-la β-Lactamase Gene from
    J. Microbiol. 2006;44(1):126-128.
    DOI: https://doi.org/2326 [pii]
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