Nucleocapsid Amino Acids 211 to 254, in Particular, Tetrad Glutamines, are Essential for the Interaction Between the Nucleocapsid and Membrane Proteins of SARS-Associated Coronavirus

Xiaonan Fang; Lin-Bai Ye; Yijuan Zhang; Baozong Li; Shanshan Li; Lingbao Kong; Yuhua Wang; Hong Zheng; Wei Wang; Zhenghui Wu

doi:2437 [pii]

Journal Article
Nucleocapsid Amino Acids 211 to 254, in Particular, Tetrad Glutamines, are Essential for the Interaction Between the Nucleocapsid and Membrane Proteins of SARS-Associated Coronavirus: Xiaonan Fang , Lin-Bai Ye , Yijuan Zhang , Baozong Li , Shanshan Li , Lingbao Kong , Yuhua Wang , Hong Zheng , Wei Wang , Zhenghui Wu; Journal of Microbiology 2006;44(5):577-580
DOI: https://doi.org/2437 [pii]

State Key Laboratory of Virology, College of Life Science, Wuhan University, Wuhan, Hubei 430072, P.R. ChinaState Key Laboratory of Virology, College of Life Science, Wuhan University, Wuhan, Hubei 430072, P.R. China

Corresponding author: Lin-Bai Ye , Tel: 86-27-6875-2372,

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Abstract

GST pull-down assays were used to characterize the SARS-CoV membrane (M) and nucleocapsid (N) interaction, and it was found that the amino acids 211-254 of N protein were essential for this interaction. When tetrad glutamines (Q) were replaced with glutamic acids (E) at positions of 240-243 of the N protein, the interaction was disrupted.

Keywords: SARS-CoV; membrane protein; nucleocapsid protein; M-N interaction，GST pull-down assay

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Nucleocapsid Amino Acids 211 to 254, in Particular, Tetrad Glutamines, are Essential for the Interaction Between the Nucleocapsid and Membrane Proteins of SARS-Associated Coronavirus

J. Microbiol. 2006;44(5):577-580.

DOI: https://doi.org/2437 [pii]

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