Comparative Proteomic Analysis of Virulent Korean Mycobacterium tuberculosis K-strain with Other Mycobacteria Strain Following Infection of U-937 Macrophage

Sung Weon Ryoo; Young Kil Park; Sue-Nie Park; Young Soo Shim; Hyunjeong Liew; Seongman Kang; Gill-Han Bai

doi:2532 [pii]

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Comparative Proteomic Analysis of Virulent Korean Mycobacterium tuberculosis K-strain with Other Mycobacteria Strain Following Infection of U-937 Macrophage: Sung Weon Ryoo ^1,2, Young Kil Park ¹, Sue-Nie Park ³, Young Soo Shim ⁴, Hyunjeong Liew ⁵, Seongman Kang ², Gill-Han Bai ¹; Journal of Microbiology 2007;45(3):268-271
DOI: https://doi.org/2532 [pii]

¹Department of Microbiology, Korean Institute of Tuberculosis, Seoul 137-140, Republic of Korea, ²Graduate School of Biotechnology, Korea University, Seoul 136-701, Republic of Korea, ³National Center for Toxicological Research, Korea Food and Drug Administration, Seoul 122-704, Republic of Korea, ⁴Department of Internal Medicine, College of Medicine, Seoul National University, Seoul 110-744, Republic of Korea, ⁵Department of Pharmacology, College of Medicine, Seoul National University, Seoul 110-744, Republic of Korea¹Department of Microbiology, Korean Institute of Tuberculosis, Seoul 137-140, Republic of Korea, ²Graduate School of Biotechnology, Korea University, Seoul 136-701, Republic of Korea, ³National Center for Toxicological Research, Korea Food and Drug Administration, Seoul 122-704, Republic of Korea, ⁴Department of Internal Medicine, College of Medicine, Seoul National University, Seoul 110-744, Republic of Korea, ⁵Department of Pharmacology, College of Medicine, Seoul National University, Seoul 110-744, Republic of Korea

Corresponding author: Gill-Han Bai , Tel: 82-2-576-4981,

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Abstract

In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDITOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.

Keywords: proteomic analysis; Mycobacterium tuberculosis K-strain; human monocytic cell line U-937; 2-D PAGE; MALDI-TOF-MS; Mb1363; MT2656

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Comparative Proteomic Analysis of Virulent Korean Mycobacterium tuberculosis K-strain with Other Mycobacteria Strain Following Infection of U-937 Macrophage

J. Microbiol. 2007;45(3):268-271.

DOI: https://doi.org/2532 [pii]

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