Anti-Tumor Activity of Acinetobacter baumannii Outer Membrane Protein A on Dendritic Cell-Based Immunotherapy against Murine Melanoma

Jun Sik Lee; Jung Wook Kim; Chul Hee Choi; Won Kee Lee; Hae Young Chung; Je Chul Lee

doi:10.1007/s12275-008-0052-z

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Research Support, Non-U.S. Gov't
Anti-Tumor Activity of Acinetobacter baumannii Outer Membrane Protein A on Dendritic Cell-Based Immunotherapy against Murine Melanoma: Jun Sik Lee ¹, Jung Wook Kim ¹, Chul Hee Choi ¹, Won Kee Lee ², Hae Young Chung ³, Je Chul Lee ¹; Journal of Microbiology 2008;46(2):221-227.
DOI: https://doi.org/10.1007/s12275-008-0052-z
Published online: June 11, 2008

¹Department of Microbiology, Kyungpook National University School of Medicine, Daegu 700-422, Republic of Korea, ²Department of Preventive Medicine, Kyungpook National University School of Medicine, Daegu 700-422, Republic of Korea, ³Department of Pharmacy, College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea

Corresponding author: Je Chul Lee , Tel: 82-53-420-4844,

Received: 20 February 2008 • Accepted: 25 March 2008

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Abstract

Acinetobacter baumannii outer membrane protein A (AbOmpA) is a major surface protein that is an important pathogen-associated molecular pattern. Based on our previous findings that AbOmpA induced the phenotypic maturation of dendritic cells (DCs) and drove the Th1 immune response in vitro, we investigated the therapeutic efficacy of AbOmpA-pulsed DC vaccines in a murine melanoma model. The surface expression of co-stimulatory molecules (CD80 and CD86) and major histocompatibility complex class I and II molecules was higher in DCs pulsed with AbOmpA alone or with a combination of B16F10 cell lysates than that of DCs pulsed with B16F10 cell lysates. AbOmpA stimulated the maturation of murine splenic DCs in vivo. In a therapeutic model of murine melanoma, AbOmpA-pulsed DCs significantly retarded tumor growth and improved the survival of tumor-bearing mice. AbOmpA-pulsed DCs significantly enhanced CD8+, interleukin-2+ T cells and CD4+, interferon-γ+ T cells in tumor-bearing mice. These results provide evidence that AbOmpA may be therapeutically useful in adjuvant DC immunotherapy against poorly immunogenic melanoma without tumor-specific antigens.

Keywords: dendritic cells;outer membrane protein;melanoma;vaccine;cytokine

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Anti-Tumor Activity of Acinetobacter baumannii Outer Membrane Protein A on Dendritic Cell-Based Immunotherapy against Murine Melanoma

J. Microbiol. 2008;46(2):221-227. Published online June 11, 2008

DOI: https://doi.org/10.1007/s12275-008-0052-z

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