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Research Support, Non-U.S. Gov't
- NOTE] Molecular Analysis of the fur (ferric uptake regulator) Gene of a Pathogenic Edwardsiella tarda Strain
- Fang Wang 1,2, Shuang Cheng 1,2, Kun Sun 1,2, Li Sun 1
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Journal of Microbiology 2008;46(3):350-355.
DOI: https://doi.org/10.1007/s12275-008-0038-x
Published online: July 5, 2008
1Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P. R. China, 2Graduate University of the Chinese Academy of Sciences, Beijing 100049, P. R. China1Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P. R. China, 2Graduate University of the Chinese Academy of Sciences, Beijing 100049, P. R. China
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Corresponding author:
Li Sun , Tel: 86-532-8289-8834,
Received: 12 February 2008 • Accepted: 18 March 2008
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Abstract
The gene encoding the Edwardsiella tarda ferric uptake regulator (FurEt) was cloned from a pathogenic E. tarda strain isolated from diseased fish. FurEt shares 90% overall sequence identity with the Escherichia coli Fur (FurEc) and was able to complement the mutant phenotype of a furEc-defective E. coli strain. Mutational analysis indicated that C92S and C95S mutations inactivated FurEt whereas E112K mutation resulted in a superactive FurEt variant. FurEt negatively regulated its own expression; interruption of this regulation impaired bacterial growth, altered the production of certain outer membrane proteins, and attenuated bacterial virulence.
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