Protective Effect of Polygoni Cuspidati Radix and Emodin on Vibrio vulnificus Cytotoxicity and Infection

Jong Ro Kim; Dool-Ri Oh; Mi Hye Cha; Byoung Sik Pyo; Joon Haeng Rhee; Hyon E. Choy; Won Keun Oh; Young Ran Kim

doi:10.1007/s12275-008-0232-x

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Protective Effect of Polygoni Cuspidati Radix and Emodin on Vibrio vulnificus Cytotoxicity and Infection: Jong Ro Kim ^1,2, Dool-Ri Oh ^1,2, Mi Hye Cha ^1,2, Byoung Sik Pyo ¹, Joon Haeng Rhee ^2,3,4, Hyon E. Choy ^2,4, Won Keun Oh ⁵, Young Ran Kim ^1,2; Journal of Microbiology 2008;46(6):737-743
DOI: https://doi.org/10.1007/s12275-008-0232-x
Published online: December 24, 2008

¹Department of Oriental Medicine Materials, Dongshin University, Jeonnam 520-714, Republic of Korea, ²Clinical Vaccine R&D Center, ³National Research Laboratory of Molecular Microbial Pathogenesis, ⁴Research Institute of Vibrio Infection and Genome Research Center for Enteropathogenic Bacteria, Chonnam National University Medical School, Gwangju 501-746, Republic of Korea, ⁵College of Pharmacy, Chosun University, Gwangju 501-759, Republic of Korea¹Department of Oriental Medicine Materials, Dongshin University, Jeonnam 520-714, Republic of Korea, ²Clinical Vaccine R&D Center, ³National Research Laboratory of Molecular Microbial Pathogenesis, ⁴Research Institute of Vibrio Infection and Genome Research Center for Enteropathogenic Bacteria, Chonnam National University Medical School, Gwangju 501-746, Republic of Korea, ⁵College of Pharmacy, Chosun University, Gwangju 501-759, Republic of Korea

Corresponding author: Young Ran Kim , Tel: 82-61-330-3262,

Received: 19 September 2008 • Accepted: 20 October 2008

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Abstract

Vibrio vulnificus, a good model organism of bacterial septicemia, causes fatal septicemia manifesting a fulminating course and a high mortality rate within days. In order to identify new natural substances preventing V. vulnificus infection, a plant library was screened for inhibiting cytotoxicity to host cells by using Trypan blue staining and LDH assay. We found that Polygoni Cuspidati Radix potently suppressed the acute death of HeLa and RAW264.7 cells in a dose dependent manner. Further studies revealed that Polygoni Cuspidati Radix inhibited V. vulnificus growth and survival in HI broth and seawater, respectively. We confirmed that Polygoni Cuspidati Radix contained high level of emodin by thin layer chromatography (TLC). Emodin showed direct antibacterial activity against V. vulnificus. In addition, emodin prevented the morphologic damages and acute death of HeLa cells caused from V. vulnificus. The safety of Polygoni Cuspidati Radix and emodin to host cells was confirmed by MTT assay. Polygoni Cuspidati Radix and emodin protected mice from V. vulnificus infection.

Keywords: V. vulnificus infection; emodin; Polygoni Cuspidati Radix; cytotoxicity; antibacterial activity

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Protective Effect of Polygoni Cuspidati Radix and Emodin on Vibrio vulnificus Cytotoxicity and Infection

J. Microbiol. 2008;46(6):737-743. Published online December 24, 2008

DOI: https://doi.org/10.1007/s12275-008-0232-x

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