Monitoring Nutrient Impact on Bacterial Community Composition during Bioremediation of Anoxic PAH-Contaminated Sediment

Myungsu Kim; Seung Seob Bae; Mijin Seol; Jung-Hyun Lee; Young-Sook Oh

doi:10.1007/s12275-008-0097-z

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Monitoring Nutrient Impact on Bacterial Community Composition during Bioremediation of Anoxic PAH-Contaminated Sediment: Myungsu Kim ¹, Seung Seob Bae ², Mijin Seol ¹, Jung-Hyun Lee ², Young-Sook Oh ¹; Journal of Microbiology 2008;46(6):615-623.
DOI: https://doi.org/10.1007/s12275-008-0097-z
Published online: December 24, 2008

¹Department of Environmental Engineering and Biotechnology, Myongji University, Yongin 449-728, Republic of Korea, ²Microbiology Laboratory, Korea Ocean Research and Development Institute, Ansan 426-744, Republic of Korea¹Department of Environmental Engineering and Biotechnology, Myongji University, Yongin 449-728, Republic of Korea, ²Microbiology Laboratory, Korea Ocean Research and Development Institute, Ansan 426-744, Republic of Korea

Corresponding author: Young-Sook Oh , Tel: 82-31-330-6691,

Received: 18 April 2008 • Accepted: 6 September 2008

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Abstract

Marine harbor sediments are frequently polluted with significant amount of polycyclic aromatic hydrocarbons (PAHs) some of which are naturally toxic, recalcitrant, mutagenic, and carcinogenic. To stimulate biodegradation of PAHs in PAH-contaminated sediments collected from near Gwangyang Bay, Korea, lactate was chosen as a supplementary carbonaceous substrate. Sediment packed into 600 ml air-tight jar was either under no treatment condition or lactate amended condition (1%, w/v). Microbial community composition was monitored by bacteria-specific and archaea-specific PCR-terminal restriction fragment length polymorphism (T-RFLP), in addition to measuring the residual PAH concentration. Results showed that lactate amendment enhanced biodegradation rate of PAHs in the sediment by 4 to 8 times, and caused a significant shift in archaebacterial community in terms of structure and diversity with time. Phylogenetic analysis of 23 archaeal clones with distinctive RFLP patterns among 288 archaeal clones indicated that majority of the archaeal members were closest to unculturable environmental rDNA clones from hydrocarbon-contaminated and/or methanogenesis-bearing sediments. Lactate amendment led to the enrichment of some clones that were most closely related to PAH-degrading Methanosarcina species. These results suggest a possible contribution of methanogenic community to PAH degradation and give us more insights on how to effectively remediate PAH-contaminated sediments.

Keywords: anaerobic biodegradation;bacterial community;archaea;polycyclic aromatic hydrocarbon;anoxic sediment

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Monitoring Nutrient Impact on Bacterial Community Composition during Bioremediation of Anoxic PAH-Contaminated Sediment

J. Microbiol. 2008;46(6):615-623. Published online December 24, 2008

DOI: https://doi.org/10.1007/s12275-008-0097-z

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