Note] Antibacterial Activity of Recombinant hCAP18/LL37 Protein Secreted from Pichia pastoris

Soon-ja Kim; Renshu Quan; Sung-Jin Lee; Hak-Kyo Lee; Joong-Kook Choi

doi:10.1007/s12275-009-0131-9

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Research Support, Non-U.S. Gov't
Note] Antibacterial Activity of Recombinant hCAP18/LL37 Protein Secreted from Pichia pastoris: Soon-ja Kim ¹, Renshu Quan ¹, Sung-Jin Lee ², Hak-Kyo Lee ³, Joong-Kook Choi ¹; Journal of Microbiology 2009;47(3):358-362
DOI: https://doi.org/10.1007/s12275-009-0131-9
Published online: June 26, 2009

¹Department of Biochemistry, College of Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea, ²Department of Animal Life Resource Science, Kangwon National University, Chuncheon 200-701, Republic of Korea, ³Gyeonggi Regional Research Center, Hankyong National University, Ansung 456-749, Republic of Korea¹Department of Biochemistry, College of Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea, ²Department of Animal Life Resource Science, Kangwon National University, Chuncheon 200-701, Republic of Korea, ³Gyeonggi Regional Research Center, Hankyong National University, Ansung 456-749, Republic of Korea

Corresponding author: Soon-ja Kim , Tel: 82-43-261-2844,

Received: 22 April 2009 • Accepted: 20 May 2009

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Abstract

Human antimicrobial peptide CAP18/LL37 (hCAP18/LL37) was expressed in Pichia pastoris and its antibacterial activity was tested against pathogenic bacteria. The full length ORF of hCAP18/LL37 was cloned into the pPICZαA vector followed by integration into the genomic AOX1 gene of P. pastoris. Agar diffusion assay demonstrated that the different hCAP18/LL37 transformants showed various antibacterial activities against Staphylococcus aureus, Micrococcus luteus, and Salmonella gastroenteritis. The secreted form of hCAP18/LL37 exhibited its maximum activity after 72 h incubation with 2% methanol in MM media, not in BMM. This result suggests that the yeast secreted expression system can be used as a production tool of antimicrobial peptides for industrial or pharmaceutical application.

Keywords: hCAP18/ LL37; antimicrobial peptide (AMP); antibacterial activity; Pichia pastoris

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Note] Antibacterial Activity of Recombinant hCAP18/LL37 Protein Secreted from Pichia pastoris

J. Microbiol. 2009;47(3):358-362. Published online June 26, 2009

DOI: https://doi.org/10.1007/s12275-009-0131-9

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