Bradyrhizobium spp. and Sinorhizobium fredii are Predominant in Root Nodules of Vigna angularis, a Native Legume Crop in the Subtropical Region of China

Li Li Han; En Tao Wang; Yang Li Lu; Yong Fa Zhang; Xin Hua Sui; Wen Feng Chen; Wen Xin Chen

doi:10.1007/s12275-009-0001-5

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Bradyrhizobium spp. and Sinorhizobium fredii are Predominant in Root Nodules of Vigna angularis, a Native Legume Crop in the Subtropical Region of China: Li Li Han ¹, En Tao Wang ^1,2, Yang Li Lu ¹, Yong Fa Zhang ^1,3, Xin Hua Sui ¹, Wen Feng Chen ¹, Wen Xin Chen ¹; Journal of Microbiology 2009;47(3):287-296
DOI: https://doi.org/10.1007/s12275-009-0001-5
Published online: June 26, 2009

¹State Key Laboratory for Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, P. R. China, ²Departamento de Microbiologia, Escuela Nacional de Ciencias Biol?icas, Instituto Politecnico Nacional, M?ico D. F. 11340, Mexico, ³Department of Food and Bioengineering Science, Henan University of Science & Technology, Luoyang 471003, P. R. China¹State Key Laboratory for Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, P. R. China, ²Departamento de Microbiologia, Escuela Nacional de Ciencias Biol?icas, Instituto Politecnico Nacional, M?ico D. F. 11340, Mexico, ³Department of Food and Bioengineering Science, Henan University of Science & Technology, Luoyang 471003, P. R. China

Corresponding author: Wen Xin Chen , Tel: 86-10-6273-4008,

Received: 17 December 2008 • Accepted: 11 March 2009

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Abstract

Adzuki bean (Vigna angularis) is an important legume crop native to China, but its rhizobia have not been well characterized. In the present study, a total of 60 rhizobial strains isolated from eight provinces of China were analyzed with amplified 16S rRNA gene RFLP, IGS-RFLP, and sequencing analyses of 16S rRNA, atpD, recA, and nodC genes. These strains were identified as genomic species within Rhizobium, Sinorhizobium, Mesorhizobium, Bradyrhizobium, and Ochrobactrum. The most abundant groups were Bradyrhizobium species and Sinorhizobium fredii. Diverse nodC genes were found in these strains, which were mainly co-evolved with the housekeeping genes, but a possible lateral transfer of nodC from Sinorhizobium to Rhizobium was found. Analyses of the genomic and symbiotic gene backgrounds showed that adzuki bean shared the same rhizobial gene pool with soybean (legume native to China) and the exotic Vigna species. All of these data demonstrated that nodule formation is the interaction of rhizobia, host plants, and environment characters.

Keywords: phylogeny; adzuki bean; rhizobia; diversity

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Bradyrhizobium spp. and Sinorhizobium fredii are Predominant in Root Nodules of Vigna angularis, a Native Legume Crop in the Subtropical Region of China

J. Microbiol. 2009;47(3):287-296. Published online June 26, 2009

DOI: https://doi.org/10.1007/s12275-009-0001-5

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