Abstract
A novel Streptomyces strain, L10, which is capable of producing natamycin, was isolated from a soil sample collected from Zhejiang province, China. On the basis of phylogenetic analysis of rpoB gene and 16S rDNA sequences, as well as phenotypic comparison, strain L10 (CGMCC 2644) is proposed to be a previously uncharacterized strain of S. chattanoogensis. By screening a cosmid library of strain L10 and primer walking, a partial sequence of scnRI and the entire sequence of scnRII were obtained, which are orthologues to the pathway-specific positive regulator genes of natamycin biosynthesis in S. natalensis. The engineered S. chattanoogensis D1, generated by inserting an additional copy of scnRII into the chromosome of strain L10, increased its natamycin production by 3.3 fold in YSG medium and 4.6 fold in YEME medium without sucrose.
Citations
Citations to this article as recorded by
- Microbial-Derived Bioactive Substances: Sources, Identification, and Application in Fruit Disease Management
Xiaomin Yang, Meng Liu, Fang Zhang, Xianghong Meng
Food Reviews International.2025; : 1. CrossRef - South African actinobacteria: A treasure trove of novel bioactive metabolites for drug discovery
Kojo S. Acquah, David W. Gammon, Denzil R. Beukes
South African Journal of Science.2024;[Epub] CrossRef - Bio-source substances against postharvest diseases of fruits: Mechanisms, applications and perspectives
Daoguo Chen, Tong Chen, Yong Chen, Zhanquan Zhang, Boqiang Li, Shiping Tian
Postharvest Biology and Technology.2023; 198: 112240. CrossRef -
Novel mechanism of hydrogen peroxide for promoting efficient natamycin synthesis in
Streptomyces
Gongli Zong, Guangxiang Cao, Jiafang Fu, Peipei Zhang, Xi Chen, Wenxiu Yan, Lulu Xin, Zhongxue Wang, Yan Xu, Rongzhen Zhang, Beile Gao
Microbiology Spectrum.2023;[Epub] CrossRef - Effect of PAS-LuxR Family Regulators on the Secondary Metabolism of Streptomyces
Naifan Zhang, Yao Dong, Hongli Zhou, Hao Cui
Antibiotics.2022; 11(12): 1783. CrossRef - MacRS controls morphological differentiation and natamycin biosynthesis in Streptomyces gilvosporeus F607
Gongli Zong, Guangxiang Cao, Jiafang Fu, Peipei Zhang, Xi Chen, Wenxiu Yan, Lulu Xin, Wenchi Zhang, Yan Xu, Rongzhen Zhang
Microbiological Research.2022; 262: 127077. CrossRef - Roles of LuxR-family regulators in the biosynthesis of secondary metabolites in Actinobacteria
Zhangqun Li, Xiaofang Li, Haiyang Xia
World Journal of Microbiology and Biotechnology.2022;[Epub] CrossRef - Use of elicitors to enhance or activate the antibiotic production in streptomyces
Gongli Zong, Jiafang Fu, Peipei Zhang, Wenchi Zhang, Yan Xu, Guangxiang Cao, Rongzhen Zhang
Critical Reviews in Biotechnology.2022; 42(8): 1260. CrossRef - A new bacterial tRNA enhances antibiotic production in Streptomyces by circumventing inefficient wobble base-pairing
Ximing Chen, Shuyan Li, Binglin Zhang, Haili Sun, Jinxiu Wang, Wei Zhang, Wenbo Meng, Tuo Chen, Paul Dyson, Guangxiu Liu
Nucleic Acids Research.2022; 50(12): 7084. CrossRef - Waste cooking oil: New efficient carbon source for natamycin production by Streptomyces gilvosporeus Z8
Xin Zeng, Chaoping Yue, Zixian Ding, Lulin Wang, Zhiwei Su, Huawei Zeng, Biao Zhang, Feng Li, Mingzhi Zhu
Process Biochemistry.2022; 118: 294. CrossRef - Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC–NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy
S. Karthick Raja Namasivayam, Mohith Manohar, J. Aravind Kumar, K. Samrat, Akhil Kande, R.S. Arvind Bharani, C. Jayaprakash, S. Lokesh
Environmental Research.2022; 212: 113386. CrossRef - Baseline Sensitivities of Major Citrus, Pome, and Stone Fruits Postharvest Pathogens to Natamycin and Estimation of the Resistance Potential in Penicillium digitatum
Daniel Chen, Helga Fӧrster, James E. Adaskaveg
Plant Disease.2021; 105(8): 2114. CrossRef - Improvement of natamycin production by controlling the morphology of Streptomyces gilvosporeus Z8 with microparticle talc in seed preculture
Chaoping Yue, Haitao Xu, Yingying Yu, Xin Yu, Min Yu, Chen Zhang, Qian You, Shaofan Xia, Zixian Ding, Hao Fu, Xin Zeng, Feng Li
Journal of Chemical Technology & Biotechnology.2021; 96(6): 1533. CrossRef - Genome mining and homologous comparison strategy for digging exporters contributing self-resistance in natamycin-producing Streptomyces strains
Yiming Shan, Dong Guo, Quanshu Gu, Yudong Li, Yongquan Li, Yinghu Chen, Wenjun Guan
Applied Microbiology and Biotechnology.2020; 104(2): 817. CrossRef - Generation of tetramycin B derivative with improved pharmacological property based on pathway engineering
Yong Sheng, Yixin Ou, Xiaojing Hu, Zixin Deng, Linquan Bai, Qianjin Kang
Applied Microbiology and Biotechnology.2020; 104(6): 2561. CrossRef - Application of Genetic Engineering Approaches to Improve Bacterial Metabolite Production
Xin Xie, Jia-Wei Zhu, Yi Liu, Hui Jiang
Current Protein & Peptide Science.2020; 21(5): 488. CrossRef - The Application of Regulatory Cascades in Streptomyces: Yield Enhancement and Metabolite Mining
Haiyang Xia, Xiaofang Li, Zhangqun Li, Xinqiao Zhan, Xuming Mao, Yongquan Li
Frontiers in Microbiology.2020;[Epub] CrossRef - Regulation of aureofuscin production by the PAS-LuxR family regulator AurJ3M
Jing Yang, Dexin Xu, Wenchen Yu, Ruobing Hao, Jie Wei
Enzyme and Microbial Technology.2020; 137: 109532. CrossRef - Production of natamycin by Streptomyces gilvosporeus Z28 through solid-state fermentation using agro-industrial residues
Xin Zeng, Wenyun Miao, Huawei Zeng, Kuo Zhao, Yaling Zhou, Juan Zhang, Qinxue Zhao, Delinur Tursun, Dayong Xu, Feng Li
Bioresource Technology.2019; 273: 377. CrossRef - Streptomycesspp from Black Pepper Rhizosphere: A Boundless Reservoir of Antimicrobial and Growth Promoting Metabolites
T. Anusree, R. Suseela Bhai, T.P. Ahammed Shabeer, Dasharath Oulkar
Journal of Biologically Active Products from Nature.2019; 9(1): 1. CrossRef - Actinomycete-Derived Polyketides as a Source of Antibiotics and Lead Structures for the Development of New Antimicrobial Drugs
Helene L. Robertsen, Ewa M. Musiol-Kroll
Antibiotics.2019; 8(4): 157. CrossRef - Transcriptome-Based Identification of a Strong Promoter for Hyper-production of Natamycin in Streptomyces
Kai Wang, Xiao-Fang Liu, Qing-Ting Bu, Yang Zheng, Xin-Ai Chen, Yong-Quan Li, Xu-Ming Mao
Current Microbiology.2019; 76(1): 95. CrossRef - Identification of a secondary metabolism-responsive promoter by proteomics for over-production of natamycin in Streptomyces
Kai Wang, Xin-Ai Chen, Yong-Quan Li, Xu-Ming Mao
Archives of Microbiology.2019; 201(10): 1459. CrossRef - Enhanced natamycin production by co-expression of Vitreoscilla hemoglobin and antibiotic positive regulators in Streptomyces gilvosporeus
Haixia Wang, Xihong He, Chunjie Sun, Junqian Gao, Xiaoguang Liu, Hao Liu
Biotechnology & Biotechnological Equipment.2018; 32(2): 470. CrossRef - Bidirectional Regulation of AdpAch in Controlling the Expression of scnRI and scnRII in the Natamycin Biosynthesis of Streptomyces chattanoogensis L10
Pin Yu, Qing-Ting Bu, Yi-Li Tang, Xu-Ming Mao, Yong-Quan Li
Frontiers in Microbiology.2018;[Epub] CrossRef -
Complete Genome Sequence of the High-Natamycin-Producing Strain
Streptomyces gilvosporeus
F607
Gongli Zong, Chuanqing Zhong, Jiafang Fu, Zhilong Zhao, Guangxiang Cao
Genome Announcements.2018;[Epub] CrossRef - Genomics-Driven Discovery of Chlorinated Cyclic Hexapeptides Ulleungmycins A and B from a Streptomyces Species
Sangkeun Son, Young-Soo Hong, Mina Jang, Kyung Taek Heo, Byeongsan Lee, Jun-Pil Jang, Jong-Won Kim, In-Ja Ryoo, Won-Gon Kim, Sung-Kyun Ko, Bo Yeon Kim, Jae-Hyuk Jang, Jong Seog Ahn
Journal of Natural Products.2017; 80(11): 3025. CrossRef - Multiple transporters are involved in natamycin efflux in Streptomyces chattanoogensis L10
Tan‐Jun Wang, Yi‐Ming Shan, Han Li, Wei‐Wang Dou, Xin‐Hang Jiang, Xu‐Ming Mao, Shui‐Ping Liu, Wen‐Jun Guan, Yong‐Quan Li
Molecular Microbiology.2017; 103(4): 713. CrossRef - Comparative Genomic and Regulatory Analyses of Natamycin Production of Streptomyces lydicus A02
Huiling Wu, Weicheng Liu, Lingling Shi, Kaiwei Si, Ting Liu, Dan Dong, Taotao Zhang, Juan Zhao, Dewen Liu, Zhaofeng Tian, Yuesen Yue, Hong Zhang, Bai Xuelian, Yong Liang
Scientific Reports.2017;[Epub] CrossRef - Physicochemical and microbial responses of Streptomyces natalensis HW-2 to fungal elicitor
Dahong Wang, Lanlan Wei, Ying Zhang, Mengjuan Zhang, Shaobin Gu
Applied Microbiology and Biotechnology.2017; 101(17): 6705. CrossRef - Directed accumulation of less toxic pimaricin derivatives by improving the efficiency of a polyketide synthase dehydratase domain
Zhen Qi, Yucong Zhou, Qianjin Kang, Chunyan Jiang, Jianting Zheng, Linquan Bai
Applied Microbiology and Biotechnology.2017; 101(6): 2427. CrossRef - Biotechnological production and application of the antibiotic pimaricin: biosynthesis and its regulation
Jesús F. Aparicio, Eva G. Barreales, Tamara D. Payero, Cláudia M. Vicente, Antonio de Pedro, Javier Santos-Aberturas
Applied Microbiology and Biotechnology.2016; 100(1): 61. CrossRef - Iteratively improving natamycin production in Streptomyces gilvosporeus by a large operon-reporter based strategy
Yemin Wang, Zhengsheng Tao, Hualiang Zheng, Fei Zhang, Qingshan Long, Zixin Deng, Meifeng Tao
Metabolic Engineering.2016; 38: 418. CrossRef - Engineering microbial hosts for production of bacterial natural products
Mingzi M. Zhang, Yajie Wang, Ee Lui Ang, Huimin Zhao
Natural Product Reports.2016; 33(8): 963. CrossRef - Target-specific identification and characterization of the putative gene cluster for brasilinolide biosynthesis revealing the mechanistic insights and combinatorial synthetic utility of 2-deoxy-l-fucose biosynthetic enzymes
Hsien-Tai Chiu, Chien-Pao Weng, Yu-Chin Lin, Kuan-Hung Chen
Organic & Biomolecular Chemistry.2016; 14(6): 1988. CrossRef - Genetic manipulation of secondary metabolite biosynthesis for improved production in Streptomyces and other actinomycetes
Richard H Baltz
Journal of Industrial Microbiology and Biotechnology.2016; 43(2-3): 343. CrossRef - Sigma factor WhiGch positively regulates natamycin production in Streptomyces chattanoogensis L10
Shui-Ping Liu, Pin Yu, Peng-Hui Yuan, Zhen-Xing Zhou, Qing-Ting Bu, Xu-Ming Mao, Yong-Quan Li
Applied Microbiology and Biotechnology.2015; 99(6): 2715. CrossRef - Functional manipulations of the tetramycin positive regulatory gene ttmRIV to enhance the production of tetramycin A and nystatin A1 in Streptomyces ahygroscopicus
Hao Cui, Xianpu Ni, Wei Shao, Jian Su, Jiaqi Su, Jun Ren, Huanzhang Xia
Journal of Industrial Microbiology and Biotechnology.2015; 42(9): 1273. CrossRef - Generation of the natamycin analogs by gene engineering of natamycin biosynthetic genes in Streptomyces chattanoogensis L10
Shui-Ping Liu, Peng-Hui Yuan, Yue-Yue Wang, Xiao-Fang Liu, Zhen-Xing Zhou, Qing-ting Bu, Pin Yu, Hui Jiang, Yong-Quan Li
Microbiological Research.2015; 173: 25. CrossRef - Transcriptome-guided identification of SprA as a pleiotropic regulator in Streptomyces chattanoogensis
Zhen-Xing Zhou, Qing-Qing Xu, Qing-Ting Bu, Shui-Ping Liu, Pin Yu, Yong-Quan Li
Applied Microbiology and Biotechnology.2015; 99(3): 1287. CrossRef - Engineered biosynthesis of pimaricin derivatives with improved antifungal activity and reduced cytotoxicity
Zhen Qi, Qianjin Kang, Chunyan Jiang, Mo Han, Linquan Bai
Applied Microbiology and Biotechnology.2015; 99(16): 6745. CrossRef - Heterologous coexpression of Vitreoscilla hemoglobin and Bacillus megaterium glucanase in Streptomyces lydicus A02 enhanced its production of antifungal metabolites
Huiling Wu, Jinjin Li, Dan Dong, Ting Liu, Taotao Zhang, Dianpeng Zhang, Weicheng Liu
Enzyme and Microbial Technology.2015; 81: 80. CrossRef - Expression of Paenibacillus polymyxa β-1,3-1,4-glucanase in Streptomyces lydicus A01 improves its biocontrol effect against Botrytis cinerea
Jinjin Li, Weicheng Liu, Lijin Luo, Dan Dong, Ting Liu, Taotao Zhang, Caige Lu, Dewen Liu, Dianpeng Zhang, Huiling Wu
Biological Control.2015; 90: 141. CrossRef - Pathway-specific regulation revisited: cross-regulation of multiple disparate gene clusters by PAS-LuxR transcriptional regulators
Cláudia M. Vicente, Tamara D. Payero, Javier Santos-Aberturas, Eva G. Barreales, Antonio de Pedro, Jesús F. Aparicio
Applied Microbiology and Biotechnology.2015; 99(12): 5123. CrossRef - Production of the antibiotic FR-008/candicidin in Streptomyces sp. FR-008 is co-regulated by two regulators, FscRI and FscRIV, from different transcription factor families
Zhilong Zhao, Zixin Deng, Xiuhua Pang, Xiu-Lan Chen, Peipei Zhang, Linquan Bai, Hao Li
Microbiology.2015; 161(3): 539. CrossRef - Characterization and Evolutionary Implications of the Triad Asp-Xxx-Glu in Group II Phosphopantetheinyl Transferases
Yue-Yue Wang, Yu-Dong Li, Jian-Bo Liu, Xin-Xin Ran, Yuan-Yang Guo, Ni-Ni Ren, Xin Chen, Hui Jiang, Yong-Quan Li, Albert Jeltsch
PLoS ONE.2014; 9(7): e103031. CrossRef -
SlnM gene overexpression with different promoters on natamycin production in Streptomyces lydicus A02
Huiling Wu, Weicheng Liu, Dan Dong, Jinjin Li, Dianpeng Zhang, Caige Lu
Journal of Industrial Microbiology and Biotechnology.2014; 41(1): 163. CrossRef - Enhancement of natamycin production on Streptomyces gilvosporeus by chromosomal integration of the Vitreoscilla hemoglobin gene (vgb)
Shaohua Wang, Fei Liu, Zhongwen Hou, Gongli Zong, Xiqiang Zhu, Peixue Ling
World Journal of Microbiology and Biotechnology.2014; 30(4): 1369. CrossRef - Propanol Addition Improves Natamycin Biosynthesis of Streptomyces natalensis
Min Li, Shouwen Chen, Junhui Li, Zhixia Ji
Applied Biochemistry and Biotechnology.2014; 172(7): 3424. CrossRef - Characterization of type II thioesterases involved in natamycin biosynthesis in Streptomyces chattanoogensis L10
Yue-Yue Wang, Xin-Xin Ran, Wei-Bin Chen, Shui-Ping Liu, Xiao-Sheng Zhang, Yuan-Yang Guo, Xin-Hang Jiang, Hui Jiang, Yong-Quan Li
FEBS Letters.2014; 588(17): 3259. CrossRef - Biosynthesis and pathway engineering of antifungal polyene macrolides in actinomycetes
Dekun Kong, Mi-Jin Lee, Shuangjun Lin, Eung-Soo Kim
Journal of Industrial Microbiology and Biotechnology.2013; 40(6): 529. CrossRef - Improvement of Natamycin Production by Engineering of Phosphopantetheinyl Transferases in Streptomyces chattanoogensis L10
Hui Jiang, Yue-Yue Wang, Xin-Xin Ran, Wei-Ming Fan, Xin-Hang Jiang, Wen-Jun Guan, Yong-Quan Li
Applied and Environmental Microbiology.2013; 79(11): 3346. CrossRef - Improvement in natamycin production by Streptomyces natalensis with the addition of short-chain carboxylic acids
Elsayed Ahmed Elsayed, Mohamed Abdel Fattah Farid, Hesham Ali El Enshasy
Process Biochemistry.2013; 48(12): 1831. CrossRef - Gamma-Butyrolactone Regulatory System of Streptomyces chattanoogensis Links Nutrient Utilization, Metabolism, and Development
Yi-Ling Du, Xue-Ling Shen, Pin Yu, Lin-Quan Bai, Yong-Quan Li
Applied and Environmental Microbiology.2011; 77(23): 8415. CrossRef - Functional conservation of PAS–LuxR transcriptional regulators in polyene macrolide biosynthesis
Javier Santos-Aberturas, Tamara D. Payero, Cláudia M. Vicente, Susana M. Guerra, Carmen Cañibano, Juan F. Martín, Jesús F. Aparicio
Metabolic Engineering.2011; 13(6): 756. CrossRef
MSK
Cite this Article
