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- Volume 58(10); October 2020
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Journal Articles
- [PROTOCOL]A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans
- Kwang-Woo Jung , Kyung-Tae Lee , Yong-Sun Bahn
- J. Microbiol. 2020;58(10):823-831. Published online September 29, 2020
- DOI: https://doi.org/10.1007/s12275-020-0341-8
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Abstract
-
Signature-tagged mutagenesis (STM) is a high-throughput
genetic technique that can be used to investigate the function
of genes by constructing a large number of mutant strains
with unique DNA identification tags, pooling them, and screening
them for a particular phenotypic trait. STM was first designed
for the identification of genes that contribute to the
virulence or infectivity of a pathogen in its host. Recently, this
method
has also been applied for the identification of mutants with specific phenotypes, such as antifungal drug resistance and proliferation. In the present study, we describe an STM
method
for the identification of genes contributing to the infectivity of Cryptococcus neoformans using a mutant library, in which each strain was tagged with a unique DNA sequence. -
Citations
Citations to this article as recorded by
- Genome-wide phenotypic profiling of transcription factors and identification of novel targets to control the virulence of Vibrio vulnificus
Dayoung Sung, Garam Choi, Minji Ahn, Hokyung Byun, Tae Young Kim, Hojun Lee, Zee-Won Lee, Ji Yong Park, Young Hyun Jung, Ho Jae Han, Sang Ho Choi
Nucleic Acids Research.2024;[Epub] CrossRef - Zinc-binding domain mediates pleiotropic functions of Yvh1 in Cryptococcus neoformans
Jae-Hyung Jin, Myung Kyung Choi, Hyun-Soo Cho, Yong-Sun Bahn
Journal of Microbiology.2021; 59(7): 658. CrossRef
- Genome-wide phenotypic profiling of transcription factors and identification of novel targets to control the virulence of Vibrio vulnificus
- Paenibacillus lycopersici sp. nov. and Paenibacillus rhizovicinus sp. nov., isolated from the rhizosphere of tomato (Solanum lycopersicum)
- Shin Ae Lee , Tae-Wan Kim , Mee-Kyung Sang , Jaekyeong Song , Soon-Wo Kwon , Hang-Yeon Weon
- J. Microbiol. 2020;58(10):832-840. Published online September 29, 2020
- DOI: https://doi.org/10.1007/s12275-020-0258-2
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Abstract
- Two Gram-stain-positive, rod-shaped, endospore-forming bacteria, designated 12200R-189T and 14171R-81T were isolated from the rhizosphere of tomato plants. The 16S rRNA gene sequence similarity between strains 12200R-189T and 14171R-81T were 97.2%. Both strains showed the highest 16S rRNA gene sequence similarities to Paenibacillus sacheonensis SY01T (96.3% and 98.0%, respectively). The genome of strain 12200R-189T was approximately 6.7 Mb in size with 5,750 protein-coding genes (CDSs) and the G + C content was 58.1 mol%, whereas that of strain 14171R-81T comprised one chromosome of 7.0 Mb and two plasmids (0.2 Mb each) with 6,595 CDSs and the G + C content was 54.5 mol%. Comparative genome analysis revealed that average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values among 12200R-189T, 14171R-81T, and other closely related species were below the cut-off levels 95% and 70%, respectively. Strain 12200R-189T grew at a temperature range of 15–40°C, pH 6.0–9.0, and 0–3% NaCl (w/v), whereas strain 14171R-81T grew at a temperature range of 10–37°C, pH 6.0– 8.0, and 0–1% NaCl (w/v). Menaquinone-7 (MK-7) was the only isoprenoid quinone detected in both strains. The predominant cellular fatty acids (> 10%) were iso-C15:0, anteiso- C15:0, and iso-C16:0. The polar lipids of strain 12200R- 189T were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), aminophospholipid (APL), phospholipid (PL), phosphatidylglycolipid (PGL), and four aminophosphoglycolipids (APGLs) and those of strain 14171R-81T were DPG, PG, PE, APL, three PLs, two PGLs, and three APGLs. Based on phylogenetic, genomic, phenotypic, and chemotaxonomic analyses, strains 12200R- 189T and 14171R-81T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus lycopersici sp. nov. and Paenibacillus rhizovicinus sp. nov. are proposed. The type strains are 12200R-189T (= KACC 19916T = CCTCC AB 2020027T) and 14171R-81T (= KACC 19915T = CCTCC AB 2020026T).
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Citations
Citations to this article as recorded by- Description and genomic characterization of Jiella flava sp. nov., isolated from Acrostichum aureum
Ming-Sheng Chen, Xiu-Long Pu, Ming-Dan Weng, Li Chen, Lan-Ying Zhu, Li Tuo
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef -
Jiella sonneratiae sp. nov., a novel endophytic bacterium isolated from bark of Sonneratia apetala
Ming-Sheng Chen, Hai-Bo Yi, Zi-Hao Huang, Xiao-Rui Yan, Xiao-Hui Chen, Xiao Ma, Zhou-Qing Zheng, Li Tuo
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef -
Paenibacillus vietnamensis sp. nov., isolated from the rhizosphere soil of Arachis hypogaea
Minh Hong Nguyen, Mai Thi Ngoc Dinh, Keun Chul Lee, Ji-Sun Kim, Thao Kim Nu Nguyen, Jung-Sook Lee
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef - Effect of exopolysaccharides of Paenibacillus polymyxa rhizobacteria on physiological and morphological variables of wheat seedlings
Irina V. Yegorenkova, Kristina V. Tregubova, Alexander I. Krasov, Nina V. Evseeva, Larisa Yu. Matora
Journal of Microbiology.2021; 59(8): 729. CrossRef
- Description and genomic characterization of Jiella flava sp. nov., isolated from Acrostichum aureum
- Fungal diversity in deep-sea sediments from Magellan seamounts environment of the western Pacific revealed by high-throughput Illumina sequencing
- Shuai Yang , Wei Xu , Yuanhao Gao , Xiaoyao Chen , Zhu-Hua Luo
- J. Microbiol. 2020;58(10):841-852. Published online September 2, 2020
- DOI: https://doi.org/10.1007/s12275-020-0198-x
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- 15 Web of Science
- 15 Crossref
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Abstract
- There are lots of seamounts globally whose primary production is disproportionally greater than the surrounding areas. Compared to other deep-sea environments, however, the seamounts environment is relatively less explored for fungal diversity. In the present study, we explored the fungal community structure in deep-sea sediments from four different stations of the Magellan seamounts environment by using high-throughput sequencing of the ITS1 region. A total of 1,897,618 ITS1 sequences were obtained. Among these sequences, fungal ITS1 sequences could be clustered into 1,662 OTUs. The majority of these sequences belonged to Ascomycota. In the genera level, the most abundant genus was Mortierella (4.79%), which was reported as a common fungal genus in soil and marine sediments, followed by Umbelopsis (3.80%), Cladosporium (2.98%), Saccharomycopsis (2.53%), Aspergillus (2.42%), Hortaea (2.36%), Saitozyma (2.20%), Trichoderma (2.12%), Penicillium (2.11%), Russula (1.86%), and Verticillium (1.40%). Most of these recovered genera belong to Ascomycota. The Bray-Curtis analysis showed that there was 37 to 85% dissimilarity of fungal communities between each two sediment samples. The Principal coordinates analysis clearly showed variations in the fungal community among different sediment samples. These results suggested that there was a difference in fungal community structures not only among four different sampling stations but also for different layers at the same station. The depth and geographical distance significantly affect the fungal community, and the effect of depth and geographical distance on the structure of the fungal community in the Magellan seamounts is basically same. Most of the fungi were more or less related to plants, these plant parasitic/symbiotic/endophytic fungi constitute a unique type of seamounts environmental fungal ecology, different from other marine ecosystems.
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Citations
Citations to this article as recorded by- Biodiversity and community structures across the Magellan seamounts and abyssal plains in the western Pacific Ocean revealed by environmental DNA metabarcoding analysis
Eun-Bi Kim, Se-Jong Ju, Yeon Jee Suh
Frontiers in Marine Science.2024;[Epub] CrossRef - Multi-omics reveal wild habitat is more favorable for the metabolite accumulation in Astragalus mongolicus
Miaoyin Dong, Hongyan Su, Jinjuan Li, Dan Zhang, Wenzhi Yao, Delong Yang, Jianhe Wei, Mengfei Li, Paul W. Paré
Industrial Crops and Products.2024; 222: 119953. CrossRef - Geographical distribution and driving force of micro-eukaryotes in the seamount sediments along the island arc of the Yap and Mariana trenches
Yue Zhang, Hongbin Liu, Ning Huang, Xiaotong Peng, Hongmei Jing, Jing Han
Microbiology Spectrum.2023;[Epub] CrossRef - Succession of Fungal Community during Outdoor Deterioration of Round Bamboo
Xiaojiao An, Shuaibo Han, Xin Ren, John Sichone, Zhiwei Fan, Xinxing Wu, Yan Zhang, Hui Wang, Wei Cai, Fangli Sun
Journal of Fungi.2023; 9(6): 691. CrossRef - Depth-Dependent Distribution of Prokaryotes in Sediments of the Manganese Crust on Nazimov Guyots of the Magellan Seamounts
Jianxing Sun, Hongbo Zhou, Haina Cheng, Zhu Chen, Jichao Yang, Yuguang Wang, Chunlei Jing
Microbial Ecology.2023; 86(4): 3027. CrossRef - Ecological and Oceanographic Perspectives in Future Marine Fungal Taxonomy
Nalin N. Wijayawardene, Don-Qin Dai, Prabath K. Jayasinghe, Sudheera S. Gunasekara, Yuriko Nagano, Saowaluck Tibpromma, Nakarin Suwannarach, Nattawut Boonyuen
Journal of Fungi.2022; 8(11): 1141. CrossRef - Lignicolous freshwater fungi in Yunnan Province, China: an overview
Hong-Wei Shen, Dan-Feng Bao, Darbhe J. Bhat, Hong-Yan Su, Zong-Long Luo
Mycology.2022; 13(2): 119. CrossRef - Microbial diversity and community structure in deep-sea sediments of South Indian Ocean
Daochen Zhu, Sivasamy Sethupathy, Lu Gao, Muhammad Zohaib Nawaz, Weimin Zhang, Jianxiong Jiang, Jianzhong Sun
Environmental Science and Pollution Research.2022; 29(30): 45793. CrossRef - Marine Fungi: Opportunities and Challenges
Micael F. M. Gonçalves, Ana C. Esteves, Artur Alves
Encyclopedia.2022; 2(1): 559. CrossRef - Effects of Environmental Factors on Fungal Diversity and Composition in Coastal Sediments from Guangdong, China
Ke-Yue Wu, Yong-Chun Liu, Li Mo, Zu-Wang Sun, Zhi-Ying Liu, Zi-Hui Chen, Ri-Ming Huang, Xiaoyong Zhang
SSRN Electronic Journal .2022;[Epub] CrossRef - Fungal Diversity and Composition of the Continental Solar Saltern in Añana Salt Valley (Spain)
Maia Azpiazu-Muniozguren, Alba Perez, Aitor Rementeria, Irati Martinez-Malaxetxebarria, Rodrigo Alonso, Lorena Laorden, Javier Gamboa, Joseba Bikandi, Javier Garaizar, Ilargi Martinez-Ballesteros
Journal of Fungi.2021; 7(12): 1074. CrossRef - Bacterial and Fungal Diversity in Sediment and Water Column From the Abyssal Regions of the Indian Ocean
Natasha Maria Barnes, Samir R. Damare, Belle Damodara Shenoy
Frontiers in Marine Science.2021;[Epub] CrossRef - Diversity, Ecological Role and Biotechnological Potential of Antarctic Marine Fungi
Stefano Varrella, Giulio Barone, Michael Tangherlini, Eugenio Rastelli, Antonio Dell’Anno, Cinzia Corinaldesi
Journal of Fungi.2021; 7(5): 391. CrossRef - Patterns of Sediment Fungal Community Dependent on Farming Practices in Aquaculture Ponds
Zhimin Zhang, Qinghui Deng, Xiuyun Cao, Yiyong Zhou, Chunlei Song
Frontiers in Microbiology.2021;[Epub] CrossRef - Metabolic Potential, Ecology and Presence of Associated Bacteria Is Reflected in Genomic Diversity of Mucoromycotina
Anna Muszewska, Alicja Okrasińska, Kamil Steczkiewicz, Olga Drgas, Małgorzata Orłowska, Urszula Perlińska-Lenart, Tamara Aleksandrzak-Piekarczyk, Katarzyna Szatraj, Urszula Zielenkiewicz, Sebastian Piłsyk, Ewa Malc, Piotr Mieczkowski, Joanna S. Kruszewska
Frontiers in Microbiology.2021;[Epub] CrossRef
- Biodiversity and community structures across the Magellan seamounts and abyssal plains in the western Pacific Ocean revealed by environmental DNA metabarcoding analysis
- Roles of Dhh1 RNA helicase in yeast filamentous growth: Analysis of N-terminal phosphorylation residues and ATPase domains
- Eunji Lee , Daehee Jung , Jinmi Kim
- J. Microbiol. 2020;58(10):853-858. Published online September 29, 2020
- DOI: https://doi.org/10.1007/s12275-020-0431-7
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Abstract
- In yeast Saccharomyces cerevisiae, the Dhh1 protein, a member of the DEAD-box RNA helicase, stimulates Dcp2/Dcp1- mediated mRNA decapping and functions as a general translation repressor. Dhh1 also positively regulates translation of a selected set of mRNAs, including Ste12, a transcription factor for yeast mating and pseudohyphal growth. Given the diverse functions of Dhh1, we investigated whether the putative phosphorylation sites or the conserved motifs for the DEADbox RNA helicases were crucial in the regulatory roles of Dhh1 during pseudohyphal growth. Mutations in the ATPase A or B motif (DHH1-K96R or DHH1-D195A) showed significant defects in pseudohyphal colony morphology and agar invasive phenotypes. The N-terminal phospho-mimetic mutation, DHH1-T16E, showed defects in pseudohyphal phenotypes. Decreased levels of Ste12 protein were also observed in these pseudohyphal-defective mutant cells under filamentous- inducing low nitrogen conditions. We suggest that the ATPase motifs and the Thr16 phosphorylation site of Dhh1 are crucial to its regulatory roles in pseudohyphal growth under low nitrogen conditions.
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Citations
Citations to this article as recorded by
- Intervention with kimchi microbial community ameliorates obesity by regulating gut microbiota
- Seong-Eun Park , Sun Jae Kwon , Kwang-Moon Cho , Seung-Ho Seo , Eun-Ju Kim , Tatsuya Unno , So-Hyeon Bok , Dae-Hun Park , Hong-Seok Son
- J. Microbiol. 2020;58(10):859-867. Published online September 2, 2020
- DOI: https://doi.org/10.1007/s12275-020-0266-2
- 46 View
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- 19 Web of Science
- 19 Crossref
-
Abstract
- The objective of this study was to evaluate anti-obesity effects of kimchi microbial community (KMC) on obesity and gut microbiota using a high fat diet-induced mouse model compared to effects of a single strain. Administration of KMC decreased body weight, adipose tissue, and liver weight gains. Relative content of Muribaculaceae in the gut of the KMCtreated group was higher than that in the high-fat diet (HFD) group whereas relative contents of Akkermansiaceae, Coriobacteriaceae, and Erysipelotrichaceae were lower in KMCtreated group. Metabolic profile of blood was found to change differently according to the administration of KMC and a single strain of Lactobacillus plantarum. Serum metabolites significantly increased in the HFD group but decreased in the KMC-treated group included arachidic acid, stearic acid, fumaric acid, and glucose, suggesting that the administration of KMC could influence energy metabolism. The main genus in KMC was not detected in guts of mice in KMC-treated group. Since the use of KMC has advantages in terms of safety, it has potential to improve gut microbial community for obese people.
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Citations
Citations to this article as recorded by- Anti-obesogenic effects of plant natural products: A focus on Korean traditional foods
Gitishree Das, Luis Alfonso Jiménez Ortega, Sandra Gonçalves, J. Basilio Heredia, Maria de Lourdes Gomes Pereira, Anabela Romano, Han-Seung Shin, Jayanta Kumar Patra
Trends in Food Science & Technology.2024; 148: 104470. CrossRef - Anti-obesity activity of lactic acid bacteria-starter-based kimchi in high-fat diet-induced obese mice
Ye-Rang Yun, Min-Sung Kwon, Ho-Jae Lee, Wooje Lee, Ji-Eun Lee, Sung Wook Hong
Journal of Functional Foods.2024; 112: 105966. CrossRef - Effects of Kimchi Intake on the Gut Microbiota and Metabolite Profiles of High-Fat-Induced Obese Rats
Dong-Wook Kim, Quynh-An Nguyen, Saoraya Chanmuang, Sang-Bong Lee, Bo-Min Kim, Hyeon-Jeong Lee, Gwang-Ju Jang, Hyun-Jin Kim
Nutrients.2024; 16(18): 3095. CrossRef - Does kimchi deserve the status of a probiotic food?
Jeongmin Cha, Yeon Bee Kim, Seong-Eun Park, Se Hee Lee, Seong Woon Roh, Hong-Seok Son, Tae Woong Whon
Critical Reviews in Food Science and Nutrition.2024; 64(19): 6512. CrossRef - Revisiting the potential anti-obesity effects of kimchi and lactic acid bacteria isolated from kimchi: a lustrum of evidence
Anshul Sharma, Hae-Jeung Lee
Journal of Ethnic Foods.2024;[Epub] CrossRef - Metabolites of Kimchi Lactic Acid Bacteria, Indole-3-Lactic Acid, Phenyllactic Acid, and Leucic Acid, Inhibit Obesity-Related Inflammation in Human Mesenchymal Stem Cells
Moeun Lee, Daun Kim, Ji Yoon Chang
Journal of Microbiology and Biotechnology.2024; 34(2): 306. CrossRef -
Dysregulation of CD177
+
neutrophils on intraepithelial lymphocytes exacerbates gut inflammation via decreasing microbiota-derived DMF
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Gut Microbes.2023;[Epub] CrossRef - MicroRNA‐29a Compromises Hepatic Adiposis and Gut Dysbiosis in High Fat Diet‐Fed Mice via Downregulating Inflammation
Ya‐Ling Yang, Ying‐Hsien Huang, Feng‐Sheng Wang, Ming‐Chao Tsai, Chien‐Hung Chen, Wei‐Shiung Lian
Molecular Nutrition & Food Research.2023;[Epub] CrossRef - Certain Fermented Foods and Their Possible Health Effects with a Focus on Bioactive Compounds and Microorganisms
Gülsüm Deveci, Elif Çelik, Duygu Ağagündüz, Elena Bartkiene, João Miguel F. Rocha, Fatih Özogul
Fermentation.2023; 9(11): 923. CrossRef - Presence of an ultra-small microbiome in fermented cabbages
Hae-Won Lee, So-Ra Yoon, Yun-Mi Dang, Miran Kang, Kwangho Lee, Ji-Hyung Ha, Jin-Woo Bae
PeerJ.2023; 11: e15680. CrossRef - Immunomodulatory effects of Companilactobacillus allii WiKim39 and Lactococcus lactis WiKim0124 isolated from kimchi on lipopolysaccharide-induced RAW264.7 cells and dextran sulfate sodium-induced colitis in mice
Ye-Rang Yun, Moeun Lee, Jung Hee Song, Eun Ji Choi, Ji Yoon Chang
Journal of Functional Foods.2022; 90: 104969. CrossRef - Interaction between the PNPLA3 Gene and Nutritional Factors on NAFLD Development: The Korean Genome and Epidemiology Study
Sooyeon Oh, Jooho Lee, Sukyung Chun, Ja-Eun Choi, Mi Na Kim, Young Eun Chon, Yeonjung Ha, Seong-Gyu Hwang, Sang-Woon Choi, Kyung-Won Hong
Nutrients.2022; 15(1): 152. CrossRef -
Acids produced by lactobacilli inhibit the growth of commensal
Lachnospiraceae
and S24-7 bacteria
Emma J. E. Brownlie, Danica Chaharlangi, Erin Oi-Yan Wong, Deanna Kim, William Wiley Navarre
Gut Microbes.2022;[Epub] CrossRef - The Role of Gut Microbiota Modulation Strategies in Obesity: The Applications and Mechanisms
Lingyue Shan, Akanksha Tyagi, Umair Shabbir, Xiuqin Chen, Selvakumar Vijayalakshmi, Pianpian Yan, Deog-Hwan Oh
Fermentation.2022; 8(8): 376. CrossRef - Fermented rice bran supplementation ameliorates obesity via gut microbiota and metabolism modification in female mice
Shiro Tochitani, Yoshiteru Maehara, Takahiro Kawase, Takamitsu Tsukahara, Ryoichi Shimizu, Taizo Watanabe, Kazuo Maehara, Kenji Asaoka, Hideo Matsuzaki
Journal of Clinical Biochemistry and Nutrition.2022; 70(2): 160. CrossRef - Bifidobacterium longum 070103 Fermented Milk Improve Glucose and Lipid Metabolism Disorders by Regulating Gut Microbiota in Mice
Tong Jiang, Ying Li, Longyan Li, Tingting Liang, Mingzhu Du, Lingshuang Yang, Juan Yang, Runshi Yang, Hui Zhao, Moutong Chen, Yu Ding, Jumei Zhang, Juan Wang, Xinqiang Xie, Qingping Wu
Nutrients.2022; 14(19): 4050. CrossRef - What Is the Role of Gut Microbiota in Obesity Prevalence? A Few Words about Gut Microbiota and Its Association with Obesity and Related Diseases
Julita Tokarek, Joanna Gadzinowska, Ewelina Młynarska, Beata Franczyk, Jacek Rysz
Microorganisms.2021; 10(1): 52. CrossRef - Eating Fermented: Health Benefits of LAB-Fermented Foods
Vincenzo Castellone, Elena Bancalari, Josep Rubert, Monica Gatti, Erasmo Neviani, Benedetta Bottari
Foods.2021; 10(11): 2639. CrossRef - Anti-Inflammatory and Immunomodulatory Properties of Fermented Plant Foods
Roghayeh Shahbazi, Farzaneh Sharifzad, Rana Bagheri, Nawal Alsadi, Hamed Yasavoli-Sharahi, Chantal Matar
Nutrients.2021; 13(5): 1516. CrossRef
- Anti-obesogenic effects of plant natural products: A focus on Korean traditional foods
- Stenotrophomonas maltophilia outer membrane protein A induces epithelial cell apoptosis via mitochondrial pathways
- Xin Wang , Yan Li , Xueping Tang , Xueyi Shang , Zunquan Zhao , Yongqiang Jiang , Yan Li
- J. Microbiol. 2020;58(10):868-877. Published online September 2, 2020
- DOI: https://doi.org/10.1007/s12275-020-0235-9
- 40 View
- 0 Download
- 9 Web of Science
- 4 Crossref
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Abstract
- Stenotrophomonas maltophilia (S. maltophilia) is a common opportunistic pathogen in intensive care units and causes infections most often after surgeries in immune-compromised patients such as those undergoing chemotherapy. Outer membrane protein A (OmpA) is the most abundant of the outer membrane proteins in S. maltophilia. Previous studies on OmpA usually focus on its interaction with the host cells and its role in vaccine development. However, the impact of OmpA on the virulence of S. maltophilia to host cells and the effects on apoptosis remain unclear. In this study, we exposed purified recombinant S. maltophilia OmpA (rOmpA) to HEp-2 cells and investigated the effects of OmpA on epithelial cell apoptosis. Morphologic and flow cytometric analyses revealed that HEp-2 cells stimulated with rOmpA multiple apoptosis features, including nuclear roundness and pyknosis, chromatin aggregation, and phosphatidylserine eversion. We found that rOmpA regulated the protein levels of Bax and Bcl-xL in HEp-2 cells, leading to changes in mitochondria permeability and the release of cytochrome c and apoptosis-inducing factors into the cytoplasm. These subsequently activate the caspase-9/caspase-3 pathway that promote apoptosis. We also observed that rOmpA enhanced the generation of reactive oxygen species and increased intracellular Ca2+ levels in HEp-2 cells. Collectively, our data suggested that rOmpA induced epithelial cells apoptosis via mitochondrial pathways.
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Citations
Citations to this article as recorded by- Functional analysis of OmpA and its contribution to pathogenesis of Edwardsiella tarda
Quan Zhou, Yushuai Hu, Yicheng You, Yingli Gao, Xingqiang Wang, Lei Qin
Microbial Pathogenesis.2024; 193: 106760. CrossRef -
Application of mPEG-CS-cRGD/
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Xiaoyan Xu, Tianhao Zhou, Xudong Wei, Xuelian Jiang, Jiyan Cao
Drug Delivery.2023;[Epub] CrossRef - Control of host mitochondria by bacterial pathogens
Saverio Marchi, Gianluca Morroni, Paolo Pinton, Lorenzo Galluzzi
Trends in Microbiology.2022; 30(5): 452. CrossRef - Lysophosphatidic acid protects cervical cancer HeLa cells from apoptosis induced by doxorubicin hydrochloride
Xibo Wang, Haihua Wang, Xiaoxiao Mou, Yilin Xu, Wenbo Han, Aimin Huang, Yanwei Li, Hui Jiang, Xiaoyun Yang, Zhenbo Hu
Oncology Letters.2022;[Epub] CrossRef
- Functional analysis of OmpA and its contribution to pathogenesis of Edwardsiella tarda
- Anti-inflammatory and anti-oxidative effect of Korean propolis on Helicobacter pylori-induced gastric damage in vitro
- Moon-Young Song , Da-Young Lee , Eun-Hee Kim
- J. Microbiol. 2020;58(10):878-885. Published online September 2, 2020
- DOI: https://doi.org/10.1007/s12275-020-0277-z
- 53 View
- 0 Download
- 28 Web of Science
- 28 Crossref
-
Abstract
- Helicobacter pylori, present in the stomach lining, is a Gramnegative bacterium that causes various gastrointestinal diseases, including gastritis and peptic ulcers. Propolis is a natural resinous substance collected from a variety of plants, and contains several natural bioactive substances. The aim of this study was to investigate the anti-inflammatory and antioxidative effects of Korean propolis on H. pylori-induced damage in the human adenocarcinoma gastric cell line. The propolis used in this study was obtained from the Korea Beekeeping Association in South Korea. The expression of pro-inflammatory interleukins (ILs), such as IL-8, IL-12, IL-1β, tumor necrosis factor alpha, cyclooxygenase-2, and inducible nitric oxide synthase, which was increased after H. pylori infection, significantly decreased in a dose-dependent manner upon pretreatment with Korean propolis, because of the suppression of mitogen-activated protein kinases and nuclear factor κB pathway. The anti-oxidative activity of propolis was assessed using the 2,2-diphenyl-1-picrylhydrazyl hydrate free radical assay. Korean propolis showed significant anti-oxidative effects via reactive oxygen species scavenging. In addition, pretreatment with Korean propolis upregulated the expression of anti-oxidant enzymes through Nrf2 signaling activation. These findings indicate that the use of Korean propolis, which has anti-inflammatory and anti-oxidative effects, can be promising for the prevention of H. pylori-induced gastric damage.
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Citations
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Liyuan Cheng, Jie Wang, Yicong Wang, Jingjing Li, Wenchao Yang
Foods.2025; 14(6): 996. CrossRef - Propolis as an autophagy modulator in relation to its roles in redox balance and inflammation regulation
R. Lesmana, S. Tandean, A. Christoper, A.A. Suwantika, N. Wathoni, R. Abdulah, J. Fearnley, V. Bankova, F. Zulhendri
Biomedicine & Pharmacotherapy.2024; 175: 116745. CrossRef - Exploring the Prospective Role of Propolis in Modifying Aging Hallmarks
Carla Scorza, Valeria Goncalves, Josef Finsterer, Fúlvio Scorza, Fernando Fonseca
Cells.2024; 13(5): 390. CrossRef - Development of a chitosan/propolis-based polymeric system: Characterization, biocompatibility, and modulation of transcription factor expression
Raquel Velázquez-Rodríguez, Amaury Pozos-Guillén, Martha Gabriela Chuc-Gamboa, Juan Valerio Cauich-Rodríguez, Héctor Flores-Reyes, Francisco Javier Tejeda-Nava, Fernando Javier Aguilar-Perez, Diana Maria Escobar Garcia
Journal of Bioactive and Compatible Polymers.2024; 39(5): 329. CrossRef - Therapeutic potential of propolis in alleviating inflammatory response and promoting wound healing in skin burn
Christian Oktavianus Manginstar, Trina Ekawati Tallei, Nurdjannah Jane Niode, Christina Leta Salaki, Sofia Safitri Hessel
Phytotherapy Research.2024; 38(2): 856. CrossRef - A review for non-antibiotic treatment of Helicobacter pylori: new insight
Neda Shadvar, Sousan Akrami, Seyyed-Mohammad-Amin Mousavi Sagharchi, Rafee Habib Askandar, Alireza Merati, Masoomeh Aghayari, Nikki Kaviani, Hamed Afkhami, Mojtaba Kashfi
Frontiers in Microbiology.2024;[Epub] CrossRef - Wound Healing, Anti-Inflammatory and Anti-Oxidant Activities, and Chemical Composition of Korean Propolis from Different Sources
Aman Dekebo, Chalshisa Geba, Daniel Bisrat, Jin Boo Jeong, Chuleui Jung
International Journal of Molecular Sciences.2024; 25(21): 11352. CrossRef - Effects of oxidative stress regulation in inflammation-associated gastric cancer progression treated using traditional Chinese medicines: A review
Bo Chen, Xinqian Dong, Jinlong Zhang, Wei Wang, Yujiao Song, Xitong Sun, Kangning Zhao, Zhen Sun
Medicine.2023; 102(46): e36157. CrossRef - Emodin Attenuates Inflammasome Activation Induced by Helicobacter pylori Infection through Inhibition of VacA Translocation
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- Development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) thermal inactivation method with preservation of diagnostic sensitivity
- Young-Il Kim , Mark Anthony B. Casel , Se-Mi Kim , Seong-Gyu Kim , Su-Jin Park , Eun-Ha Kim , Hye Won Jeong , Haryoung Poo , Young Ki Choi
- J. Microbiol. 2020;58(10):886-891. Published online September 29, 2020
- DOI: https://doi.org/10.1007/s12275-020-0335-6
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Abstract
-
Various treatments and agents had been reported to inactivate
RNA viruses. Of these, thermal inactivation is generally
considered an effective and cheap method of sample
preparation for downstream assays. The purpose of this study
is to establish a safe inactivation method for SARS-CoV-2
without compromising the amount of amplifiable viral genome
necessary for clinical diagnoses. In this study, we demonstrate
the infectivity and genomic stability of SARSCoV-
2 by thermal inactivation at both 56°C and 65°C. The
results
substantiate that viable SARS-CoV-2 is readily inactivated when incubated at 56°C for 30 min or at 65°C for 10 min. qRT-PCR of specimens heat-inactivated at 56°C for 30 min or 65°C for 15 min revealed similar genomic RNA stability compared with non-heat inactivated specimens. Further, we demonstrate that 30 min of thermal inactivation at 56°C could inactivate viable viruses from clinical COVID-19 specimens without attenuating the qRT-PCR diagnostic sensitivity. Heat treatment of clinical specimens from COVID-19 patients at 56°C for 30 min or 65°C for 15 min could be a useful
method
for the inactivation of a highly contagious agent, SARS-CoV-2. Use of this method would reduce the potential for secondary infections in BSL2 conditions during diagnostic procedures. Importantly, infectious virus can be inactivated in clinical specimens without compromising the sensitivity of the diagnostic RT-PCR assay. -
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