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- [PROTOCOL]A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans
- Kwang-Woo Jung 1, Kyung-Tae Lee 2, Yong-Sun Bahn 2
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Journal of Microbiology 2020;58(10):823-831
DOI: https://doi.org/10.1007/s12275-020-0341-8
Published online: September 29, 2020
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Corresponding author:
Yong-Sun Bahn , Tel: +82-2-2123-5558,
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Abstract
Signature-tagged mutagenesis (STM) is a high-throughput
genetic technique that can be used to investigate the function
of genes by constructing a large number of mutant strains
with unique DNA identification tags, pooling them, and screening
them for a particular phenotypic trait. STM was first designed
for the identification of genes that contribute to the
virulence or infectivity of a pathogen in its host. Recently, this
method
has also been applied for the identification of mutants
with specific phenotypes, such as antifungal drug resistance
and proliferation. In the present study, we describe an STM
method
for the identification of genes contributing to the infectivity
of Cryptococcus neoformans using a mutant library,
in which each strain was tagged with a unique DNA sequence.
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