[PROTOCOL]A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans

Kwang-Woo Jung; Kyung-Tae Lee; Yong-Sun Bahn

doi:10.1007/s12275-020-0341-8

Articles

Page Path: HOME > J. Microbiol > Volume 58(10); 2020 > Article

Journal Article
[PROTOCOL]A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans: Kwang-Woo Jung ¹, Kyung-Tae Lee ², Yong-Sun Bahn ²; Journal of Microbiology 2020;58(10):823-831.
DOI: https://doi.org/10.1007/s12275-020-0341-8
Published online: September 29, 2020

¹Radiation Research Division, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea, ²Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea¹Radiation Research Division, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea, ²Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea

Corresponding author: Yong-Sun Bahn , Tel: +82-2-2123-5558,

Received: 6 July 2020 • Accepted: 12 August 2020

57 Views
0 Download
2 Web of Science
2 Crossref
1 Scopus

Full Article

Abstract

Signature-tagged mutagenesis (STM) is a high-throughput genetic technique that can be used to investigate the function of genes by constructing a large number of mutant strains with unique DNA identification tags, pooling them, and screening them for a particular phenotypic trait. STM was first designed for the identification of genes that contribute to the virulence or infectivity of a pathogen in its host. Recently, this
method
has also been applied for the identification of mutants with specific phenotypes, such as antifungal drug resistance and proliferation. In the present study, we describe an STM
method
for the identification of genes contributing to the infectivity of Cryptococcus neoformans using a mutant library, in which each strain was tagged with a unique DNA sequence.

Keywords: EVD68;apoptosis;caspase-3;host-pathogen interaction;viral production

Figure & Data

References

Citations

Citations to this article as recorded by

Genome-wide phenotypic profiling of transcription factors and identification of novel targets to control the virulence of Vibrio vulnificus
Dayoung Sung, Garam Choi, Minji Ahn, Hokyung Byun, Tae Young Kim, Hojun Lee, Zee-Won Lee, Ji Yong Park, Young Hyun Jung, Ho Jae Han, Sang Ho Choi
Nucleic Acids Research.2024;[Epub] CrossRef
Zinc-binding domain mediates pleiotropic functions of Yvh1 in Cryptococcus neoformans
Jae-Hyung Jin, Myung Kyung Choi, Hyun-Soo Cho, Yong-Sun Bahn
Journal of Microbiology.2021; 59(7): 658. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

[PROTOCOL]A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans

J. Microbiol. 2020;58(10):823-831. Published online September 29, 2020

DOI: https://doi.org/10.1007/s12275-020-0341-8

Related articles