- Volume 51(5); October 2013
-
Review
- MINIREVIEW] Bioactive Activities of Natural Products against Herpesvirus Infection
-
Myoungki Son , Minjung Lee , Gi-Ho Sung , Taeho Lee , Yu Su Shin , Hyosun Cho , Paul M. Lieberman , Hyojeung Kang
-
J. Microbiol. 2013;51(5):545-551. Published online October 31, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3450-9
-
-
43
View
-
0
Download
-
33
Crossref
-
Abstract
-
More than 90% of adults have been infected with at least one human herpesvirus, which establish long-term latent infection for the life of the host. While anti-viral drugs exist that limit herpesvirus replication, many of these are ineffective against latent infection. Moreover, drug-resistant strains of herpesvirus emerge following chemotherapeutic treatment. For example, resistance to acyclovir and related nucleoside analogues can occur when mutations arise in either HSV thymidine kinase or DNA polymerases. Thus, there exists an unmet medical need to develop new anti-herpesvirus agents with different mechanisms of action. In this Review, we discuss the promise of anti-herpetic substances derived from natural products including extracts and pure compounds from potential herbal medicines. One example is Glycyrrhizic acid isolated from licorice that shows promising antiviral activity towards human gammaherpesviruses. Secondly, we discuss anti-herpetic mechanisms utilized by several natural products in molecular level. While nucleoside analogues inhibit replicating herpesviruses in lytic replication, some natural products can disrupt the herpesvirus latent infection in the host cell. In addition, natural products can stimulate immune responses against herpesviral infection. These findings suggest that natural products could be one of the best choices for development of new treatments for latent herpesvirus infection, and may provide synergistic anti-viral activity when supplemented with nucleoside analogues. Therefore, it is important to identify which natural products are more efficacious anti-herpetic agents, and to understand the molecular mechanism in detail for further advance in the anti-viral therapies.
-
Citations
Citations to this article as recorded by

- Exploring phytobiotics in aquaculture: sources, mode of action, effects, administration, and its bioavailability in fish
Pandi Kalaiselvan, Kavitha Malarvizhi, Amit Ranjan
Aquaculture International.2024; 32(5): 5737. CrossRef - Tilapia lake virus: understanding incidence, enhancing resistance, and exploring seaweed therapies
Sourabh Debbarma, Nitesh Kumar Yadav, Ng Chinglembi Devi, Bagthasingh Chrisolite, Yilbong Yirang, Dharmendra Kumar Meena, Soibam Khogen Singh, Naresh Kumar Mehta, Bhargavi Priyadarshini Mocherla
Aquaculture International.2024; 32(7): 10313. CrossRef - Causality between herpes virus infections and allograft dysfunction after tissue and organ transplantation: a two-sample bidirectional Mendelian randomization study
Xiaojuan Qiu, Tianjiao Ma, Shishun Zhao, Zongyu Zheng
Frontiers in Immunology.2024;[Epub] CrossRef - Association between human herpesviruses and multiple sclerosis: A systematic review and meta-analysis
Zohreh Khalesi, Vahid Tamrchi, Mohammad Hossein Razizadeh, Arash Letafati, Pouya Moradi, Arezoo Habibi, Negar Habibi, Jafar Heidari, Maryam Noori, Mahboubeh Nahid Samiei, Ziba Azarash, Mahdiyeh Hoseini, Hassan Saadati, Atefeh Bahavar, Maryam Farajzade, Se
Microbial Pathogenesis.2023; 177: 106031. CrossRef - Delivery of miR-34a-5p by Folic Acid-Modified β-Cyclodextrin-Grafted Polyethylenimine Copolymer Nanocarriers to Resist KSHV
Fangling Li, Dongdong Cao, Wenyi Gu, Lin Cui, Zhongpeng Qiu, Zhiyong Liu, Dongmei Li, Xuhong Guo
ACS Applied Nano Materials.2023; 6(12): 10826. CrossRef - Exploration of natural compounds against the human mpox virus DNA-dependent RNA polymerase in silico
Jameel M. Abduljalil, Abdo A. Elfiky, Alaa M. Elgohary
Journal of Infection and Public Health.2023; 16(7): 996. CrossRef - Tackling the Future Pandemics: Broad-Spectrum Antiviral Agents (BSAAs) Based on A-Type Proanthocyanidins
Massimo E. Maffei, Cristiano Salata, Giorgio Gribaudo
Molecules.2022; 27(23): 8353. CrossRef - The Role of the Natural Antioxidant Mechanism in Sperm Cells
Alicja Kowalczyk
Reproductive Sciences.2022; 29(5): 1387. CrossRef - Viral infections in cultured fish and shrimps: current status and treatment methods
Haimanti Mondal, Natarajan Chandrasekaran, Amitava Mukherjee, John Thomas
Aquaculture International.2022; 30(1): 227. CrossRef - Microalgal drugs: A promising therapeutic reserve for the future
Ankesh Ahirwar, Khushboo Kesharwani, Rahul Deka, Shreya Muthukumar, Mohd. Jahir Khan, Anshuman Rai, Vandana Vinayak, Sunita Varjani, Khashti Ballabh Joshi, Shruti Morjaria
Journal of Biotechnology.2022; 349: 32. CrossRef - Palmitic Amide Triggers Virus Life Cycle via Enhancing Host Energy Metabolism
Xinyi Zhang, Jianjian Zhuang, Liquan Huang, Xiaobo Zhang
Frontiers in Microbiology.2022;[Epub] CrossRef - 2-Methoxy-6-Acetyl-7-Methyljuglone: A Bioactive Phytochemical with Potential Pharmacological Activities
Atif Ali Khan Khalil, Asma Saleem Qazi, Abdul Nasir, Mi-Jeong Ahn, Muhammad Ajmal Shah, Muhammad Saad Ahmad, Wasim Sajjad, Tahir Ali, Muhammad Naeem, Fawad Ali Shah, Muhammad Tufail Ahmed Khan, Muhammad Romman, Shahfiq Ur Rehman, Adnan Haider, Raishma
Anti-Cancer Agents in Medicinal Chemistry.2022; 22(4): 687. CrossRef - Natural Products and Their Derivatives against Human Herpesvirus Infection
Chattarin Ruchawapol, Man Yuan, Si-Min Wang, Wen-Wei Fu, Hong-Xi Xu
Molecules.2021; 26(20): 6290. CrossRef - Antiviral activity of sulfated polysaccharides from Sargassum ilicifolium against fish Betanodavirus infection
Jyotsna, Parameswaran Vijayakumar, Tharmathass Stalin Dhas, Ravi Mani, Vasantharaja Raguraman
Aquaculture International.2021; 29(3): 1049. CrossRef - Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen
Hongtao Wang, Ping Lu, Chongshan Yuan, Jing Zhao, Hongyu Liu, Wenfa Lu, Jun Wang
Animals.2021; 11(6): 1506. CrossRef - Viral UL8 Is Involved in the Antiviral Activity of Oleanolic Acid Against HSV-1 Infection
Tianhao Shan, Ju Ye, Jiaoyan Jia, Zhaoyang Wang, Yuzhou Jiang, Yiliang Wang, Yifei Wang, Kai Zheng, Zhe Ren
Frontiers in Microbiology.2021;[Epub] CrossRef - Allicin and Glycyrrhizic Acid Display Antiviral Activity Against Latent and Lytic Kaposi Sarcoma-associated Herpesvirus
Yirui Xie, Ying Chen, Yongzheng Guo, Ying Huang, Biao Zhu
Infectious Microbes and Diseases.2020; 2(1): 30. CrossRef - Anti-herpes simplex type-1 (HSV-1) activity from the roots of Jatropha multifida L
Ratchanaporn Chokchaisiri, Jaranwit Srijun, Waraluck Chaichompoo, Sarot Cheenpracha, Lucksagoon Ganranoo, Apichart Suksamrarn
Medicinal Chemistry Research.2020; 29(2): 328. CrossRef - In Vitro Anti-HSV-1 Activity of Polyphenol-Rich Extracts and Pure Polyphenol Compounds Derived from Pistachios Kernels (Pistacia vera L.)
Maria Musarra-Pizzo, Rosamaria Pennisi, Ichrak Ben-Amor, Antonella Smeriglio, Giuseppina Mandalari, Maria Teresa Sciortino
Plants.2020; 9(2): 267. CrossRef - Oleandrin: A bioactive phytochemical and potential cancer killer via multiple cellular signaling pathways
Nazia Kanwal, Azhar Rasul, Ghulam Hussain, Haseeb Anwar, Muhammad Ajmal Shah, Iqra Sarfraz, Ammara Riaz, Rabia Batool, Muhammad Shahbaz, Arif Hussain, Zeliha Selamoglu
Food and Chemical Toxicology.2020; 143: 111570. CrossRef - A pilot study of oral tranexamic acid and Glycyrrhizin compound in the treatment of recalcitrant Riehl’s melanosis
Zhongyi Xu, Xiaoxue Xing, Chengfeng Zhang, Li Chen, Leihong Flora Xiang
Journal of Cosmetic Dermatology.2019; 18(1): 286. CrossRef - Using glycyrrhizic acid to target sumoylation processes during Epstein-Barr virus latency
Gretchen L. Bentz, Angela J. Lowrey, Dustin C. Horne, Vy Nguyen, Austin R. Satterfield, Tabithia D. Ross, Abigail E. Harrod, Olga N. Uchakina, Robert J. McKallip, Luwen Zhang
PLOS ONE.2019; 14(5): e0217578. CrossRef - The Antimicrobial and Antiviral Activity of Polyphenols from Almond (Prunus dulcis L.) Skin
Maria Musarra-Pizzo, Giovanna Ginestra, Antonella Smeriglio, Rosamaria Pennisi, Maria Teresa Sciortino, Giuseppina Mandalari
Nutrients.2019; 11(10): 2355. CrossRef - Virucidal, antiviral and immunomodulatory activities of β-escin and Aesculus hippocastanum extract
Flavia M Michelini, Laura E Alché, Carlos A Bueno
Journal of Pharmacy and Pharmacology.2018; 70(11): 1561. CrossRef - Perspective on Biotransformation and De Novo Biosynthesis of Licorice Constituents
Yujia Zhao, Bo Lv, Xudong Feng, Chun Li
Journal of Agricultural and Food Chemistry.2017; 65(51): 11147. CrossRef - Fraxinus: A Plant with Versatile Pharmacological and Biological Activities
Iqra Sarfraz, Azhar Rasul, Farhat Jabeen, Tahira Younis, Muhammad Kashif Zahoor, Muhammad Arshad, Muhammad Ali, Rainer W. Bussmann
Evidence-Based Complementary and Alternative Medicine.2017;[Epub] CrossRef - In vitro cytotoxicity and biological activities of Genipa americana (Rubiaceae) ethanolic extracts
Silva Campos Codignoto Paola, Batista de Araújo Shirley, Munhoz Bastos Natan, de Oliveira Fernandes Thais, Arielle Santana Barbosa Tamiris, Eduardo Dias Igidio Carlos, Faustino Fernando, Judite Bittencourt Fernandes Maria, Oliveira da Conceição Aline
African Journal of Microbiology Research.2017; 11(9): 385. CrossRef - Apigenin inhibits African swine fever virus infection in vitro
Astghik Hakobyan, Erik Arabyan, Aida Avetisyan, Liana Abroyan, Lina Hakobyan, Hovakim Zakaryan
Archives of Virology.2016; 161(12): 3445. CrossRef - Genipin Enhances Kaposi’s Sarcoma-Associated Herpesvirus Genome Maintenance
Miyeon Cho, Seok Won Jung, Soomin Lee, Kuwon Son, Gyu Hwan Park, Jong-Wha Jung, Yu Su Shin, Taegun Seo, Hyosun Cho, Hyojeung Kang, Shou-Jiang Gao
PLOS ONE.2016; 11(10): e0163693. CrossRef - Genipin as a novel chemical activator of EBV lytic cycle
Myoungki Son, Minjung Lee, Eunhyun Ryu, Aree Moon, Choon-Sik Jeong, Yong Woo Jung, Gyu Hwan Park, Gi-Ho Sung, Hyosun Cho, Hyojeung Kang
Journal of Microbiology.2015; 53(2): 155. CrossRef - Seaweed extracts as antimicrobial agents in aquaculture
Ioannis N Vatsos, Celine Rebours
Journal of Applied Phycology.2015; 27(5): 2017. CrossRef - Bioactive natural products with anti-herpes simplex virus properties
Sherif T S Hassan, Radka Masarčíková, Kateřina Berchová
Journal of Pharmacy and Pharmacology.2015; 67(10): 1325. CrossRef - A Novel Tricyclic Polyketide, Vanitaracin A, Specifically Inhibits the Entry of Hepatitis B and D Viruses by Targeting Sodium Taurocholate Cotransporting Polypeptide
Manabu Kaneko, Koichi Watashi, Shinji Kamisuki, Hiroki Matsunaga, Masashi Iwamoto, Fumihiro Kawai, Hirofumi Ohashi, Senko Tsukuda, Satomi Shimura, Ryosuke Suzuki, Hideki Aizaki, Masaya Sugiyama, Sam-Yong Park, Takayoshi Ito, Naoko Ohtani, Fumio Sugawara,
Journal of Virology.2015; 89(23): 11945. CrossRef
Research Support, Non-U.S. Gov'ts
- Morphological and Genetic Characteristics of Newly Crossbred Cauliflower Mushroom (Sparassis latifolia)
-
Hong-Duck Sou , Rhim Ryoo , Sung-Ryul Ryu , Kang-Hyeon Ka , Hyun Park , Sung-Hyun Joo
-
J. Microbiol. 2013;51(5):552-557. Published online June 25, 2013
-
DOI: https://doi.org/10.1007/s12275-013-2666-z
-
-
44
View
-
0
Download
-
6
Crossref
-
Abstract
-
Cauliflower mushroom (Sparassis latifolia or S. crispa) is popular for food and medicine. Importance of new varieties of Sparassis was raised and studied widely by protection system of UPOV. In this study, 10 crossbred strains of Sparassis latifolia that specifically expressed distinctive features during basidiocarp formation and mycelium growth were applied to sawdust medium inoculated with S. latifolia mycelia. The 10 crossbred strains were divided into 3 groups on the basis of morphological (size of marginal wave and basidiocarp color) and genetic characteristics. Each phenotype of the parent and crossbred strains represented 3 marginal wave-sizes (large, medium, and small) and 3 color notations (NN155D, 163C, and 8D). Our result suggests that morphological characteristics of cauliflower mushroom can be affected by various environmental and genetic stimuli under artificial conditions such as crossbreed. Also this research showed genetic differences among breeding isolates and their morphological characteristics were correlated with the molecular data within parent and crossed strain.
-
Citations
Citations to this article as recorded by

- Neuroprotective Effects of Sparassis crispa Ethanol Extract through the AKT/NRF2 and ERK/CREB Pathway in Mouse Hippocampal Cells
Malk Eun Pak, Wei Li
Journal of Fungi.2023; 9(9): 910. CrossRef - Breeding of a high-yield strain for commercial cultivation by crossing Pholiota adiposa
Chengbo Rong, Shuang Song, Li Yang, Xuejiao Pan, Yu Liu, Shouxian Wang
Ciência e Agrotecnologia.2021;[Epub] CrossRef - Elicitor-induced β-glucan contents in fruit body of cauliflower mushroom (Sparassis latifolia)
Rhim Ryoo, Hong-Duck Sou, Kang-Hyeon Ka, Hyun Park
Forest Science and Technology.2018; 14(3): 119. CrossRef - Development of a highly productive strain of Pleurotus tuoliensis for commercial cultivation by crossbreeding
Shouxian Wang, Shuang Zhao, Zhenxing Huang, Limin Yin, Jie Hu, Jianghong Li, Yu Liu, Chengbo Rong
Scientia Horticulturae.2018; 234: 110. CrossRef - Effects of Sparassis crispa in Medical Therapeutics: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Le Thi Nhu Ngoc, You-Kwan Oh, Young-Jong Lee, Young-Chul Lee
International Journal of Molecular Sciences.2018; 19(5): 1487. CrossRef - The mycelial growth and ligninolytic enzyme activity of cauliflower mushroom (Sparassis latifolia)
Hong-Duck Sou, Rhim Ryoo, Kang-Hyeon Ka, Hyun Park
Forest Science and Technology.2017; 13(4): 158. CrossRef
- Bacterial Diversity and Composition of an Alkaline Uranium Mine Tailings-Water Interface
-
Nurul H. Khan , Viorica F. Bondici , Prabhakara G. Medihala , John R. Lawrence , Gideon M. Wolfaardt , Jeff Warner , Darren R. Korber
-
J. Microbiol. 2013;51(5):558-569. Published online September 14, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3075-z
-
-
45
View
-
0
Download
-
11
Crossref
-
Abstract
-
The microbial diversity and biogeochemical potential associated with a northern Saskatchewan uranium mine watertailings interface was examined using culture-dependent and -independent techniques. Morphologically-distinct colonies from uranium mine water-tailings and a reference lake (MC) obtained using selective and non-selective media were selected for 16S rRNA gene sequencing and identification, revealing that culturable organisms from the uranium tailings interface were dominated by Firmicutes and Betaproteobacteria; whereas, MC organisms mainly consisted of Bacteroidetes and Gammaproteobacteria. Ion Torrent (IT) 16S rRNA metagenomic analysis carried out on extracted DNA from tailings and MC interfaces demonstrated the dominance of Firmicutes in both of the systems. Overall, the tailings-water interface environment harbored a distinct bacterial community relative to the MC, reflective of the ambient conditions (i.e., total dissolved solids, pH, salinity, conductivity, heavy metals) dominating the uranium tailings system. Significant correlations among the physicochemical data and the major bacterial groups present in the tailings and MC were also observed. Presence of sulfate reducing bacteria demonstrated by culture-dependent analyses and the dominance of Desulfosporosinus spp. indicated by Ion Torrent analyses within the tailings-water interface suggests the existence of anaerobic microenvironments along with the potential for reductive metabolic processes.
-
Citations
Citations to this article as recorded by

- Compost, plants and endophytes versus metal contamination: choice of a restoration strategy steers the microbiome in polymetallic mine waste
Martina Kracmarova-Farren, Jakub Papik, Ondrej Uhlik, John Freeman, Andrea Foster, Mary-Cathrine Leewis, Courtney Creamer
Environmental Microbiome.2023;[Epub] CrossRef - Concurrent reductive decontamination of chromium (VI) and uranium (VI) in groundwater by Fe(0)-based autotrophic bioprocess
Jianping Lu, Rongyue Geng, Han Zhang, Zhen Yu, Tao Chen, Baogang Zhang
Journal of Hazardous Materials.2023; 452: 131222. CrossRef - Impact of anoxic conditions, uranium(VI) and organic phosphate substrate on the biogeochemical potential of the indigenous bacterial community of bentonite
Cristina Povedano-Priego, Fadwa Jroundi, Margarita Lopez-Fernandez, Mar Morales-Hidalgo, Inés Martin-Sánchez, F. Javier Huertas, Mark Dopson, Mohamed L. Merroun
Applied Clay Science.2022; 216: 106331. CrossRef - The remediation of uranium-contaminated groundwater via bioreduction coupled to biomineralization with different pH and electron donors
Guohua Wang, Ying Liu, Jiali Wang, Jinjing Xiang, Taotao Zeng, Shiyou Li, Jian Song, Zhiyue Zhang, Jinxiang Liu
Environmental Science and Pollution Research.2022; 30(9): 23096. CrossRef - Uranium sequestration abilities of Bacillus bacterium isolated from an alkaline mining region
Uday Kumar Banala, Nilamadhab Prasad Indradyumna Das, Subba Rao Toleti
Journal of Hazardous Materials.2021; 411: 125053. CrossRef - Alkaliphilic bacteria retrieved from uranium mining effluent: Characterization, U sequestration and remediation potential
Uday Kumar Banala, Nilamadhab Prasad Indradyumna Das, Ranjib Kumar Padhi, Subba Rao Toleti
Environmental Technology & Innovation.2021; 24: 101893. CrossRef - Geochemistry of uranium mill tailings in the Athabasca Basin, Saskatchewan, Canada: A review
Jared Robertson, M. Jim Hendry, T. Kotzer, Kebbi A. Hughes
Critical Reviews in Environmental Science and Technology.2019; 49(14): 1237. CrossRef - In vitro biomedicinal properties of Pyrrolidine-2,4-Dione derived from a novel actinobacterium Streptomyces rochei, a green approach
Mookkan Bodhaguru, Prakash Santhiyagu, Mariselvam Lakshmanan, Ramasubburayan Ramasamy, Ahila Natarajan Kumari, Kannapiran Ethiraj, Palavesam Arunachalam, Immanuel Grasian
Biocatalysis and Agricultural Biotechnology.2019; 20: 101244. CrossRef - Nutrients Availability Shapes Fungal Community Composition and Diversity in the Rare Earth Mine Tailings of Southern Jiangxi, China
Genhe He, Xiaodong Wang, Xu Liu, Xiaoyu Xiao, Shoucheng Huang, Jichun Wu
Russian Journal of Ecology.2018; 49(6): 524. CrossRef - Biogeochemical Importance of the Bacterial Community in Uranium Waste Deposited at Key Lake, Northern Saskatchewan
Viorica F. Bondici, George D. W. Swerhone, James J. Dynes, John R. Lawrence, Gideon M. Wolfaardt, Jeff Warner, Darren R. Korber
Geomicrobiology Journal.2016; 33(9): 807. CrossRef - Biogeochemical activity of microbial biofilms in the water column overlying uranium mine tailings
V.F. Bondici, N.H. Khan, G.D.W. Swerhone, J.J. Dynes, J.R. Lawrence, E. Yergeau, G.M. Wolfaardt, J. Warner, D.R. Korber
Journal of Applied Microbiology.2014; 117(4): 1079. CrossRef
- Analysis of Bacterial Diversity in Sponges Collected off Chujado, an Island in Korea, Using Barcoded 454 Pyrosequencing: Analysis of a Distinctive Sponge Group Containing Chloroflexi
-
In-Hye Jeong , Kyoung-Ho Kim , Jin-Sook Park
-
J. Microbiol. 2013;51(5):570-577. Published online October 31, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3426-9
-
-
51
View
-
0
Download
-
12
Crossref
-
Abstract
-
The bacterial diversity of 14 sponges belonging to 5 different orders that were collected around Chuja Island, Korea was investigated using barcoded 454 pyrosequencing. The sponges contained many unidentified bacterial groups (e.g. more than half of the taxa at the family level) that were known only in environmental sequences and obtained from culture-independent methods. Five of the sponges were clustered into one notable group (CF group), which was distinguished from the other sponges in accordance with bacterial composition (the other sponges may be separated into more groups but clustering is not clear). The CF group contained high amounts of Chloroflexi (25.0–47.7%) and moderate amounts of Gemmatimonadetes (2.3–7.0%), AncK6 (0.6–2.2%), PAUC34f (0.8–6.0%), Acidobacteria (3.7–9.6%), and SBR1093 (1.8–5.6%) exclusively or almost exclusively to this group. Sponges in the CF group also showed higher diversity (e.g. Shannon index) than the other sponges and contained group-specific taxonomic lineages (e.g. class or family level) from group-specific phyla and even from the Proteobacteria and Actinobacteria, which were detected in all sponges at the phylum level. The CF group may be one of the most distinctive groups in sponges in terms of bacterial diversity.
-
Citations
Citations to this article as recorded by

- The effect of environmental and anthropogenic factors on the microbiome of the sponge, Halichondria panicea, at three coastal sites with different bathing water quality in North east England
Sakinah Al-Haddad, Gary S. Caldwell, Anthony S. Clare
Aquatic Sciences.2025;[Epub] CrossRef - Diversity and functional roles of the symbiotic microbiome associated to marine sponges off Karah Island, Terengganu, Malaysia
Yuki Hayami, Logajothiswaran Ambalavanan, Sandra Catherine Zainathan, Muhd Danish-Daniel, Noor Emilia Sharifah, Shumpei Iehata
Regional Studies in Marine Science.2023; 62: 102967. CrossRef - Pyrosequencing reveals sponge specific bacterial communities in marine sponges of Red Sea, Saudi Arabia
Fehmida Bibi, Sana Akhtar Alvi, Abdulmohsin Al-Sofyani, Muhammad Imran Naseer, Muhammad Yasir, Esam Ibraheem Azhar
Saudi Journal of Biological Sciences.2020; 27(1): 67. CrossRef - First Record of Microbiomes of Sponges Collected From the Persian Gulf, Using Tag Pyrosequencing
Akram Najafi, Maryam Moradinasab, Iraj Nabipour
Frontiers in Microbiology.2018;[Epub] CrossRef - Prokaryote composition and predicted metagenomic content of two Cinachyrella Morphospecies and water from West Papuan Marine Lakes
Daniel F R Cleary, Ana R M Polónia, Nicole J de Voogd
FEMS Microbiology Ecology.2018;[Epub] CrossRef - Metabolic Roles of Uncultivated Bacterioplankton Lineages in the Northern Gulf of Mexico “Dead Zone”
J. Cameron Thrash, Kiley W. Seitz, Brett J. Baker, Ben Temperton, Lauren E. Gillies, Nancy N. Rabalais, Bernard Henrissat, Olivia U. Mason, Mary Ann Moran
mBio.2017;[Epub] CrossRef - Intestinal microecology associated with fluoride resistance capability of the silkworm (Bombyx mori L.)
Guan-Nan Li, Xue-Juan Xia, Wen-Chao Tang, Yong Zhu
Applied Microbiology and Biotechnology.2016; 100(15): 6715. CrossRef - In four shallow and mesophotic tropical reef sponges from Guam the microbial community largely depends on host identity
Georg Steinert, Michael W. Taylor, Peter Deines, Rachel L. Simister, Nicole J. de Voogd, Michael Hoggard, Peter J. Schupp
PeerJ.2016; 4: e1936. CrossRef - Effect of long-term different fertilization on bacterial community structures and diversity in citrus orchard soil of volcanic ash
Jae Ho Joa, Hang Yeon Weon, Hae Nam Hyun, Young Chull Jeun, Sang Wook Koh
Journal of Microbiology.2014; 52(12): 995. CrossRef - Two distinct microbial communities revealed in the sponge Cinachyrella
Marie L. Cuvelier, Emily Blake, Rebecca Mulheron, Peter J. McCarthy, Patricia Blackwelder, Rebecca L. Vega Thurber, Jose V. Lopez
Frontiers in Microbiology.2014;[Epub] CrossRef - Analysis of bacterial diversity in sponges collected from chuuk and kosrae islands in micronesia
In-Hye Jeong, Kyoung-Ho Kim, Hyi-Seung Lee, Jin-Sook Park
Journal of Microbiology.2014; 52(1): 20. CrossRef - Bacterial Diversity of the South Pacific Sponge, Dactylospongia metachromia Based on DGGE Fingerprinting
In-Hye Jeong, Jin-Sook Park
The Korean Journal of Microbiology.2013; 49(4): 377. CrossRef
Research Support, U.S. Gov't, Non-P.H.S.
- Combined Effect of Microbial and Chemical Control Agents on Subterranean Termites
-
Maureen S. Wright , Alan R. Lax
-
J. Microbiol. 2013;51(5):578-583. Published online September 14, 2013
-
DOI: https://doi.org/10.1007/s12275-013-2628-5
-
-
42
View
-
0
Download
-
5
Crossref
-
Abstract
-
Termite mortality was measured when fungi were combined with bacteria or a chemical termiticide to determine whether a synergistic effect occurred. The fungus Beauveria bassiana was combined with the non-repellant chemical termiticide imidacloprid. Of the three B. bassiana strains tested one, B. bassiana ATCC 90519, was sufficiently pathogenic on its own that the advantage of a supplementary chemical treatment was marginal. The mortality caused by another fungal strain, B. bassiana ATCC 26037, was improved in combination with imidacloprid at both of the tested chemical concentrations over the first 14 days. The remaining fungal strain, B. bassiana ATCC 90518, demonstrated an overall mortality rate in combination with imidacloprid of 82.5%, versus a rate of 65.0% for the fungus alone. The fungus Isaria fumosorosea (Ifr) was combined with the bacterium Bacillus thuringiensis (Bt). On day 5, Ifr, Bt, and the combined treatment at a 106 spores or cells/ml dosage caused 8.8%, 22.5%, and 15.0% mortality, respectively. The Bt and combined mortality rates are not significantly different. Control mortality on day 5 was 5.0%. On day 13 the combined 106 treatment mortality rate was 91.3%, which was significantly higher than all other treatments: control at 17.5%, Ifr at 36.3% and Bt at 35.0%. When Ifr and Bt were applied at a 109 spores or cells/ml dosage, Ifr alone caused a mortality rate of 97.5% as early as day 5. The combination with Bt could not significantly increase the effectiveness of this dosage. These data demonstrate the potential for synergistic effects of fungal and chemical treatment methods, thereby broadening the use of microbial control agents and reducing the quantity of chemical agents necessary to effect control.
-
Citations
Citations to this article as recorded by

- RNAi-mediated silencing of transferrin promotes entomopathogens lethality in Odontotermes formosanus (Shiraki)
Zhiqiang Wang, Yujingyun Zhou, Fang Tang
Pesticide Biochemistry and Physiology.2024; 205: 106149. CrossRef - Termite management by entomopathogenic fungi: Recent advances and future prospects
Ali Hassan, Zhiqiang Li, Xuguo Zhou, Jianchu Mo, Qiuying Huang
Current Research in Biotechnology.2024; 7: 100183. CrossRef - Termites and Chinese agricultural system: applications and advances in integrated termite management and chemical control
Farhan Ahmad, Hatem Fouad, Shi‐You Liang, Yin Hu, Jian‐Chu Mo
Insect Science.2021; 28(1): 2. CrossRef - Influence of Zwitterionic Buffer Effects with Thermal Modification Treatments of Wood on Symbiotic Protists in Reticulitermes grassei Clément
Sónia Duarte, Lina Nunes, Davor Kržišnik, Miha Humar, Dennis Jones
Insects.2021; 12(2): 139. CrossRef - The potential ofIsariaspp. as a bioinsecticide for the biological control ofNasutitermes corniger
Rosineide da Silva Lopes, Geiziquele de Lima, Maria Tereza dos Santos Correia, Antonio Félix da Costa, Elza Áurea de Luna Alves Lima, Vera Lúcia de Menezes Lima
Biocontrol Science and Technology.2017; 27(9): 1038. CrossRef
Research Support, Non-U.S. Gov'ts
- Live/Dead State Is Not the Factor Influencing Adhesion Ability of Bifidobacterium animalis KLDS2.0603
-
Li-Qun Wang , Feng Zhao , Fei Liu , Xiang-Chen Meng
-
J. Microbiol. 2013;51(5):584-589. Published online September 14, 2013
-
DOI: https://doi.org/10.1007/s12275-013-2632-9
-
-
48
View
-
0
Download
-
12
Crossref
-
Abstract
-
Two essential requirements for probiotic bifidobacteria are that they be “live” and have “colonization” ability, following FAO/WHO guideline recommendations. The amount of research on the adhesion ability of bifidobacteria compares poorly with that of other probiotic bacteria, such as lactobacilli. The aim of the present study was to determine how gastrointestinal conditions affect the adhesion ability of bifidobacteria, and to investigate the relationship between the adhesion ability and the live/dead state of bifidobacteria. The adhesion ability of Bifidobacterium animalis KLDS2.0603 that had been subjected to the digestive enzymes, pepsin, trypsin, and proteinase K, was decreased significantly, but these treatments did not significantly change the strain’s survival rates, which were 98.78%, 97.60%, and 97.63% respectively. B. animalis KLDS2.0603 subjected to LiCl retained its adhesion ability but had a lower survival rate (59.28%) than the control group (P<0.01). B. animalis KLDS 2.0603 subjected to sodium metaperiodate exhibited higher adhesion ability than the control group (P<0.01), but the bacterial cells were killed totally. The results of transmission electron microscopy and laser scanning confocal microscopy showed that live/dead state of bifidobacteria was not one of the main factors that affected the adhesion ability of bifidobacteira, and that the substances affecting the adhesion ability of bifidobacteria were on the outer surface layer of the bifidobacterial cells. Our results also indicated that the substances related to the adhesion ability of bifidobacteria are proteinaceous. The above results will help us to understand the adhesion and colonization processes of bifidobacteria in the human gastrointestinal tract.
-
Citations
Citations to this article as recorded by

- Alteration of the gut microbiome in patients with heart failure: A systematic review and meta-analysis
Jiayi Huang, Yongping Lin, Xiangwei Ding, Song Lin, Xin Li, Wei Yan, Minglong Chen
Microbial Pathogenesis.2024; 192: 106647. CrossRef - The Effects of Cellular Membrane Damage on the Long-Term Storage and Adhesion of Probiotic Bacteria in Caco-2 Cell Line
Jakub Kiepś, Wojciech Juzwa, Anna Olejnik, Anna Sip, Jolanta Tomaszewska-Gras, Radosław Dembczyński
Nutrients.2023; 15(15): 3484. CrossRef - Mechanism of Blood Cholesterol-Lowering Action of Parabiotics
Min Cheol Kim, Min-Goo Kim
Food Supplements and Biomaterials for Health.2023;[Epub] CrossRef - The Role of the PFNA Operon of Bifidobacteria in the Recognition of Host’s Immune Signals: Prospects for the Use of the FN3 Protein in the Treatment of COVID-19
Venera Z. Nezametdinova, Roman A. Yunes, Marina S. Dukhinova, Maria G. Alekseeva, Valery N. Danilenko
International Journal of Molecular Sciences.2021; 22(17): 9219. CrossRef - Comparison of microbial diversity in rumen and small intestine of Xinong Saanen dairy goats using 16S rRNA gene high-throughput sequencing
Cong Li, Yanan Geng, Ping Wang, Huaiping Shi, Jun Luo
Animal Production Science.2021; 62(14): 1379. CrossRef - Adhesion mechanisms of Bifidobacterium animalis subsp. lactis JCM 10602 to dietary fiber
Maria TANIGUCHI, Minori NAMBU, Yoshio KATAKURA, Shino YAMASAKI-YASHIKI
Bioscience of Microbiota, Food and Health.2021; 40(1): 59. CrossRef - Potential Immunomodulatory Activity of a Selected Strain Bifidobacterium bifidum H3-R2 as Evidenced in vitro and in Immunosuppressed Mice
Jiacui Shang, Feng Wan, Le Zhao, Xiangchen Meng, Bailiang Li
Frontiers in Microbiology.2020;[Epub] CrossRef - Antimicrobial and Immunomodulatory Effects of Bifidobacterium Strains: A Review
Hyun Jung Lim, Hea Soon Shin
Journal of Microbiology and Biotechnology.2020; 30(12): 1793. CrossRef - Using probiotics for type 2 diabetes mellitus intervention: Advances, questions, and potential
Zhongke Sun, Xuejiao Sun, Juan Li, Zhaoyang Li, Qingwei Hu, Lili Li, Xinqi Hao, Maoping Song, Chengwei Li
Critical Reviews in Food Science and Nutrition.2020; 60(4): 670. CrossRef - Effects of prebiotics on immunologic indicators and intestinal microbiota structure in perioperative colorectal cancer patients
Xiaoliang Xie, Yaqin He, Hai Li, Dong Yu, Li Na, Ting Sun, Dong Zhang, Xinrong Shi, Yuhan Xia, Tao Jiang, Shikuo Rong, Shaoqi Yang, Xiaoqiang Ma, Guangxian Xu
Nutrition.2019; 61: 132. CrossRef - Probiotic properties of Enterococcus strains isolated from traditional naturally fermented cream in China
Lidong Guo, Tingting Li, Yaru Tang, Lijie Yang, Guicheng Huo
Microbial Biotechnology.2016; 9(6): 737. CrossRef - Identification of surface-associated proteins of Bifidobacterium animalis ssp. lactis KLDS 2.0603 by enzymatic shaving
Dequan Zhu, Yu Sun, Fei Liu, Aili Li, Limei Yang, Xiang-Chen Meng
Journal of Dairy Science.2016; 99(7): 5155. CrossRef
- Alternative Mechanism for the Evaluation of Indole-3-Acetic Acid (IAA) Production by Azospirillum brasilense Strains and Its Effects on the Germination and Growth of Maize Seedlings
-
Oscar Masciarelli , Lucia Urbani , Herminda Reinoso , Virginia Luna
-
J. Microbiol. 2013;51(5):590-597. Published online September 14, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3136-3
-
-
44
View
-
0
Download
-
32
Crossref
-
Abstract
-
We evaluated the production of indole-3-acetic acid (IAA) by Azospirillum brasilense strains in vitro (cell culture supernatants) and in vivo (stems and roots of maize seedlings) to clarify the role of this phytohormone as a signaling and effector molecule in the symbiotic interaction between maize and A. brasilense. The three strains all showed IAA production when cultured in NFb medium supplemented with 100 μg/ml L-tryptophan. The level of IAA production was 41.5 μg/ml for Yu62, 12.9 μg/ml for Az39, and 0.15 μg/ml for ipdC-. The release of IAA into culture medium by the bacteria appeared to be the main activator of the early growth promotion observed in the inoculated maize seedlings. The application of supernatants with different IAA contents caused significant differences in the seedling growth. This observation provides the basis for novel technological tools for effective quality control procedures on inoculants. The approach described can be incorporated into different inoculation methods, including line sowing, downspout, and foliar techniques, and increase the sustainability of symbiotic plant-bacteria systems.
-
Citations
Citations to this article as recorded by

- Enhancing Growth in Vigna radiata through the Inhibition of Charcoal Rot Disease: A Strategic Approach Using Plant Growth-Promoting Rhizobacteria
Imran Khan, Sahar Ghulam Mohyuddin, Sohail, Shah Zaman, Muhammad Qadir, Juxian Guo, Guihua Li
Microorganisms.2024; 12(9): 1852. CrossRef - Auxin-Mediated Modulation of Maize Rhizosphere Microbiome: Insights from Azospirillum Inoculation and Indole-3-Acetic Acid Treatment
Anahí Coniglio, Giovanni Larama, Sofía Nievas, Natalie L. Cale, Verónica Mora, Daniela Torres, Gastón Lopez, Florencia Donadio, Belén Rodriguez, Anelis Marin, Matias Rovere, Patricio Javier Barra, Mark F. Belmonte, Emanuel Maltempi de Souza, Fabricio Cass
Journal of Soil Science and Plant Nutrition.2024; 24(4): 6906. CrossRef - Growth and metabolism enhancement in microalgae co-cultured in suspension with the bacterium Azospirillum brasilense under heterotrophic conditions
Francisco J. Choix, Oskar A. Palacios, Claudia A. Contreras, José Carlos Espinoza-Hicks, Pedro Mondragón-Cortez, Jony R. Torres
Journal of Applied Phycology.2023; 35(1): 57. CrossRef - In Furrow Co-inoculation of Rhizobia and Azospirilla Influences the Growth and Productivity of the Common Bean
Matheus Messias, Princewill Chukwuma Asobia, Enderson Petrônio de Brito Ferreira
International Journal of Plant Production.2023; 17(4): 637. CrossRef - Effect of the Exogenous Application of Different Concentrations of Indole-3-Acetic Acid as a Growth Regulator on Onion (Allium cepa L.) Cultivation
Carlos Solano, Adriana Artola, Raquel Barrena, Cindy Ballardo, Antoni Sánchez
Agronomy.2023; 13(9): 2204. CrossRef - Pseudomonas and Curtobacterium Strains from Olive Rhizosphere Characterized and Evaluated for Plant Growth Promoting Traits
Martino Schillaci, Aida Raio, Fabiano Sillo, Elisa Zampieri, Shahid Mahmood, Muzammil Anjum, Azeem Khalid, Mauro Centritto
Plants.2022; 11(17): 2245. CrossRef - Optimization of cultural conditions using response surface methodology and modeling of indole-3-acetic acid production by Saccharothrix texasensis MB15
Abderrahmane Benadjila, Miyada Zamoum, Lamia Aouar, Abdelghani Zitouni, Yacine Goudjal
Biocatalysis and Agricultural Biotechnology.2022; 39: 102271. CrossRef - Microbiological quality analysis of inoculants based on Bradyrhizobium spp. and Azospirillum brasilense produced “on farm” reveals high contamination with non-target microorganisms
Camila Rafaeli Bocatti, Eduara Ferreira, Renan Augusto Ribeiro, Ligia Maria de Oliveira Chueire, Jakeline Renata Marçon Delamuta, Renata Katsuko Takayama Kobayashi, Mariangela Hungria, Marco Antonio Nogueira
Brazilian Journal of Microbiology.2022; 53(1): 267. CrossRef - Using plant growth-promoting microorganisms (PGPMs) to improve plant development under in vitro culture conditions
Daniel Cantabella, Ramon Dolcet-Sanjuan, Neus Teixidó
Planta.2022;[Epub] CrossRef - Synergism of Bradyrhizobium and Azospirillum baldaniorum improves growth and symbiotic performance in lima bean under salinity by positive modulations in leaf nitrogen compounds
Ágda Lorena de Oliveira Lopes, Ingrid Silva Setubal, Vicente Paulo da Costa Neto, Jerri Edson Zilli, Artenisa Cerqueira Rodrigues, Aurenivia Bonifacio
Applied Soil Ecology.2022; 180: 104603. CrossRef - Application of Azospirillum on seeds and leaves, associated with Rhizobium inoculation, increases growth and yield of common bean
Letícia Dambroz Filipini, Fernanda Kokowicz Pilatti, Edenilson Meyer, Barbara Santos Ventura, Cledimar Rogério Lourenzi, Paulo Emílio Lovato
Archives of Microbiology.2021; 203(3): 1033. CrossRef - Chickpea (Cicer arietinum L.) as model legume for decoding the co-existence of Pseudomonas fluorescens and Mesorhizobium sp. as bio-fertilizer under diverse agro-climatic zones
Sharon Nagpal, Poonam Sharma, Asmita Sirari, K.C. Kumawat, Leela Wati, S.C. Gupta, Kamalpreet Singh Mandahal
Microbiological Research.2021; 247: 126720. CrossRef - Enhancement in yield and nutritive qualities of strawberry fruits by the application of organic manures and biofertilizers
Yogesh Kumar Negi, Paramjeet Sajwan, Shweta Uniyal, A.C. Mishra
Scientia Horticulturae.2021; 283: 110038. CrossRef - Zinc solubilizing bacteria (Bacillus megaterium) with multifarious plant growth promoting activities alleviates growth in Capsicum annuum L.
Kalpana Bhatt, Dinesh Kumar Maheshwari
3 Biotech.2020;[Epub] CrossRef - What Did We Learn From Plant Growth-Promoting Rhizobacteria (PGPR)-Grass Associations Studies Through Proteomic and Metabolomic Approaches?
Dayane Alberton, Glaucio Valdameri, Vivian Rotuno Moure, Rose Adele Monteiro, Fabio de Oliveira Pedrosa, Marcelo Müller-Santos, Emanuel Maltempi de Souza
Frontiers in Sustainable Food Systems.2020;[Epub] CrossRef - Chlorothalonil tolerance of indole producing bacteria associated to wheat (Triticum turgidum L.) rhizosphere in the Yaqui Valley, Mexico
Alondra M. Díaz Rodríguez, Fannie I. Parra Cota, Gustavo Santoyo, Sergio de los Santos Villalobos
Ecotoxicology.2019; 28(5): 569. CrossRef - Decoding multifarious role of cow dung bacteria in mobilization of zinc fractions along with growth promotion of C. annuum L.
Kalpana Bhatt, Dinesh Kumar Maheshwari
Scientific Reports.2019;[Epub] CrossRef - Co-existence of Leclercia adecarboxylata (LSE-1) and Bradyrhizobium sp. (LSBR-3) in nodule niche for multifaceted effects and profitability in soybean production
K. C. Kumawat, Poonam Sharma, Inderjeet Singh, Asmita Sirari, B. S. Gill
World Journal of Microbiology and Biotechnology.2019;[Epub] CrossRef - Sulfur-oxidizing buffalo dung bacteria enhance growth and yield of Foeniculum vulgare Mill.
Sandhya Dhiman, Ramesh Chand Dubey, Dinesh Kumar Maheshwari, Sandeep Kumar
Canadian Journal of Microbiology.2019; 65(5): 377. CrossRef - Application of potassium-solubilising Proteus mirabilis MG738216 inhabiting cattle dung in improving nutrient use efficiency of Foeniculum vulgare Mill.
Sandhya Dhiman, Ramesh Chand Dubey, Nitin Baliyan, Sandeep Kumar, Dinesh Kumar Maheshwari
Environmental Sustainability.2019; 2(4): 401. CrossRef - Potential of native cold tolerant plant growth promoting bacilli to enhance nutrient use efficiency and yield of Amaranthus hypochondriacus
Chitra Pandey, Yogesh Kumar Negi, D. K. Maheshwari, Deepa Rawat, Deepti Prabha
Plant and Soil.2018; 428(1-2): 307. CrossRef - Revealing strategies of quorum sensing in Azospirillum brasilense strains Ab-V5 and Ab-V6
Josiane Fukami, Julia Laura Fernandes Abrantes, Pablo del Cerro, Marco Antonio Nogueira, Francisco Javier Ollero, Manuel Megías, Mariangela Hungria
Archives of Microbiology.2018; 200(1): 47. CrossRef - Genetic and Phenotypic Characterization of Indole-Producing Isolates of Pseudomonas syringae pv. actinidiae Obtained From Chilean Kiwifruit Orchards
Oriana Flores, Camila Prince, Mauricio Nuñez, Alejandro Vallejos, Claudia Mardones, Carolina Yañez, Ximena Besoain, Roberto Bastías
Frontiers in Microbiology.2018;[Epub] CrossRef - Co‐inoculation with Bradyrhizobium and Azospirillum Increases Yield and Quality of Soybean Seeds
Carlos Henrique Queiroz Rego, Fernanda Brito Cardoso, Ana Carina da Silva Cândido, Paulo Eduardo Teodoro, Charline Zaratin Alves
Agronomy Journal.2018; 110(6): 2302. CrossRef - MIR166a Affects the Germination of Somatic Embryos in Larixleptolepis by Modulating IAA Biosynthesis and Signaling Genes
Zhe-Xin Li, Li-Feng Zhang, Wan-Feng Li, Li-Wang Qi, Su-Ying Han
Journal of Plant Growth Regulation.2017; 36(4): 889. CrossRef - Assessment of affinity and specificity of Azospirillum for plants
Lily Pereg, Luz E. de-Bashan, Yoav Bashan
Plant and Soil.2016; 399(1-2): 389. CrossRef - Growth enhancement and drought tolerance of hybrid poplar upon inoculation with endophyte consortia
Zareen Khan, Hyungmin Rho, Andrea Firrincieli, Shang Han Hung, Virginia Luna, Oscar Masciarelli, Soo-Hyung Kim, Sharon L Doty
Current Plant Biology.2016; 6: 38. CrossRef - Morphoagronomic and productive traits of RR soybean due to inoculation via Azospirillum brasilense groove
Mario Zuffo Alan, Teodoro Bruzi Adriano, Milanez de Rezende Pedro, Cristina Bianchi Mariane, Vinicius Zambiazzi Everton, Oliveri Soares Igor, Belchior Marchetti Ribeiro Augusto, Leite Dias Vilela Guilherme
African Journal of Microbiology Research.2016; 10(13): 438. CrossRef - Foliar application of Azospirillum brasilense in soybean and seed physiological quality
Mario Zuffo Alan, Teodoro Bruzi Adriano, Milanez de Rezende Pedro, Laene Moreira de Carvalho Maria, Vinicius Zambiazzi Everton, Oliveri Soares Igor, Barroso Silva Karina
African Journal of Microbiology Research.2016; 10(20): 675. CrossRef - Composition and activity of endophytic bacterial communities in field-grown maize plants inoculated with Azospirillum brasilense
Emilyn Emy Matsumura, Vinícius Andrade Secco, Renata Stolf Moreira, Odair José Andrade Pais dos Santos, Mariangela Hungria, André Luiz Martinez de Oliveira
Annals of Microbiology.2015; 65(4): 2187. CrossRef - Indole: a signaling molecule or a mere metabolic byproduct that alters bacterial physiology at a high concentration?
Jisun Kim, Woojun Park
Journal of Microbiology.2015; 53(7): 421. CrossRef - A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulation
Oscar Masciarelli, Analía Llanes, Virginia Luna
Microbiological Research.2014; 169(7-8): 609. CrossRef
Journal Article
- Cyclooxygenase Inhibitors Reduce Biofilm Formation and Yeast-Hypha Conversion of Fluconazole Resistant Candida albicans
-
E. Abdelmegeed , Mona Ibrahim Shaaban
-
J. Microbiol. 2013;51(5):598-604. Published online September 14, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3052-6
-
-
42
View
-
0
Download
-
26
Crossref
-
Abstract
-
The incidence of fluconazole-resistant Candida albicans has been increasing worldwide. Both biofilm and fungal morphogenesis are main virulence factors of C. albicans cells. Extracellular fungal prostaglandins are synthesized during biofilm adhesion and development and through yeast-hypha conversion. Hence, we targeted prostaglandin synthesis with various cyclooxygenase (COX) inhibitors (aspirin, diclofenac, ketoprofen, tenoxicam, and ketorolac) and assessed their effect on fungal adhesion, biofilm formation, and yeast-hypha conversion in clinical isolates of Fluconazole resistant C. albicans. Significant reduction in fungal adhesion and detachment of mature biofilm was attained down to 1 mM concentrations of anti-inflammatory agents. Microscopical examination of fungal cells in the presence of the tested drugs showed significant reduction of germ tube formation. Therefore, COX inhibitors have a significant effect on reduction of Candida adhesion and biofilm development in correlation with fungal morphogenesis. Moreover, inhibition of C. albicans by COX inhibitors gave synergistic activity with fluconazole suggesting that combination therapeutic strategies may be fruitful for management of infection of Fluconazole resistant C. albicans.
-
Citations
Citations to this article as recorded by

- Hyphal‐associated protein expression is crucial for Candida albicans‐induced eicosanoid biosynthesis in immune cells
Jana Schimanski, Mark S. Gresnigt, Elena Brunner, Oliver Werz, Bernhard Hube, Ulrike Garscha
European Journal of Immunology.2024;[Epub] CrossRef - Can nonsteroidal anti-inflammatory drugs (NSAIDs) be repurposed for fungal infection?
Fatemeh Babaei, Mohammadreza Mirzababaei, Alireza Tavakkoli, Marjan Nassiri-Asl, Hossein Hosseinzadeh
Naunyn-Schmiedeberg's Archives of Pharmacology.2024; 397(1): 59. CrossRef - Elucidation of the mechanisms of fluconazole resistance and repurposing treatment options against urinary Candida spp. isolated from hospitalized patients in Alexandria, Egypt
Hend Zeitoun, Rawan A. Salem, Nadia M. El-Guink, Nesrin S. Tolba, Nelly M. Mohamed
BMC Microbiology.2024;[Epub] CrossRef - An integrated transcriptomic and metabolomic approach to investigate the heterogeneous Candida albicans biofilm phenotype
Christopher Delaney, Bryn Short, Ranjith Rajendran, Ryan Kean, Karl Burgess, Craig Williams, Carol A. Munro, Gordon Ramage
Biofilm.2023; 5: 100112. CrossRef -
Targeting Adhesion in Fungal Pathogen
Candida Albicans
Harlei Martin, Kevin Kavanagh, Trinidad Velasco-Torrijos
Future Medicinal Chemistry.2021; 13(3): 313. CrossRef - Design, synthesis and biological evaluation of a series of iron and copper chelating deferiprone derivatives as new agents active against Candida albicans
Martina Bortolami, Fabiana Pandolfi, Antonella Messore, Daniele Rocco, Marta Feroci, Roberto Di Santo, Daniela De Vita, Roberta Costi, Paola Cascarino, Giovanna Simonetti, Luigi Scipione
Bioorganic & Medicinal Chemistry Letters.2021; 42: 128087. CrossRef - Aspirin as an Antifungal-Lock Agent in Inhibition of Candidal Biofilm Formation in Surgical Catheters
Alice Kit Ying Chan, Yiu Cheung Tsang, Chun Hung Chu, Chiu Shun Peter Tsang
Infection and Drug Resistance.2021; Volume 14: 1427. CrossRef - Drug repurposing strategies in the development of potential antifungal agents
Qian Zhang, Fangyan Liu, Meng Zeng, Yingyu Mao, Zhangyong Song
Applied Microbiology and Biotechnology.2021; 105(13): 5259. CrossRef - Anti-biofilm effect by the combined action of fluconazole and acetylsalicylic acid against species of Candida parapsilosis complex
Franz de Assis Graciano dos Santos, Melyna Chaves Leite-Andrade, Ildnay de Sousa Brandão, Adryelle Idalina da Silva Alves, Maria Daniela Silva Buonafina, Michellangelo Nunes, Luiz Nascimento de Araújo-Neto, Maria Audilene de Freitas, Fábio André Brayner,
Infection, Genetics and Evolution.2020; 84: 104378. CrossRef - Diclofenac exhibits synergism with azoles against planktonic cells and biofilms of Candida tropicalis
Raimunda Sâmia Nogueira Brilhante, Jaiane Alves Brasil, Jonathas Sales de Oliveira, Vandbergue Santos Pereira, Waldemiro de Aquino Pereira-Neto, José Júlio Costa Sidrim, Marcos Fábio Gadelha Rocha
Biofouling.2020; 36(5): 528. CrossRef - Drug Repurposing Strategy against Fungal Biofilms
Thaís Pereira de Mello, Laura Nunes Silva, Lívia de Souza Ramos, Heloísa Freire Frota, Marta Helena Branquinha, André Luis Souza dos Santos
Current Topics in Medicinal Chemistry.2020; 20(7): 509. CrossRef - Aspirin alleviates orthopedic implant‑associated infection
Yi Jiang, Sheng‑Nan Wang, Hang‑Tian Wu, Han‑Jun Qin, Ming‑Liang Ren, Jian‑Chun Lin, Bin Yu
International Journal of Molecular Medicine.2019;[Epub] CrossRef - Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains
Eric Hong Jian Wong, Chow Goon Ng, Khean Lee Goh, Jamuna Vadivelu, Bow Ho, Mun Fai Loke
Scientific Reports.2018;[Epub] CrossRef - Effects of Rhodomyrtus tomentosa extract on virulence factors of Candida albicans and human neutrophil function
Jutharat Hmoteh, Khadar Syed Musthafa, Supayang Piyawan Voravuthikunchai
Archives of Oral Biology.2018; 87: 35. CrossRef -
Prostaglandin E
2
Receptor Antagonist with Antimicrobial Activity against Methicillin-Resistant Staphylococcus aureus
Mélanie A. C. Ikeh, Paul L. Fidel, Mairi C. Noverr
Antimicrobial Agents and Chemotherapy.2018;[Epub] CrossRef - Oral hygiene might prevent cancer
Oscar J. Cordero, Rubén Varela-Calviño
Heliyon.2018; 4(10): e00879. CrossRef - Characterization of a novel antibiofilm effect of nitric oxide-releasing aspirin (NCX-4040) on Candida albicans isolates from denture stomatitis patients
Francisco Madariaga-Venegas, Roberto Fernández-Soto, Luisa Fernanda Duarte, Nicole Suarez, Daniela Delgadillo, José A. Jara, Ricardo Fernández-Ramires, Blanca Urzúa, Alfredo Molina-Berríos, Sompop Bencharit
PLOS ONE.2017; 12(5): e0176755. CrossRef - Potential Antifungal Targets against a Candida Biofilm Based on an Enzyme in the Arachidonic Acid Cascade—A Review
Xinning Liu, Decai Wang, Cuixiang Yu, Tao Li, Jianqiao Liu, Shujuan Sun
Frontiers in Microbiology.2016;[Epub] CrossRef - Neutrophils influx and proinflammatory cytokines inhibition by sodium salicylate, unlike aspirin, in Candida albicans-induced peritonitis model
Priscilla Aparecida Tártari Pereira, Daniel Bini, Fernanda Bovo, Lucia Helena Faccioli, Marta Chagas Monteiro
Folia Microbiologica.2016; 61(4): 337. CrossRef - New frontiers for anti-biofilm drug development
Suzana M. Ribeiro, Mário R. Felício, Esther Vilas Boas, Sónia Gonçalves, Fabrício F. Costa, Ramar Perumal Samy, Nuno C. Santos, Octávio L. Franco
Pharmacology & Therapeutics.2016; 160: 133. CrossRef - Effect of tyrosol on Staphylococcus aureus antimicrobial susceptibility, biofilm formation and virulence factors
H Abdel Rhman Shaymaa, E Rizk Dina
African Journal of Microbiology Research.2016; 10(20): 687. CrossRef - Effect of non-steroidal anti-inflammatory drugs and dexamethazone on the biofilm formation and expression of some adhesion-related genes of Candida albicans and Staphylococcus aureus
Mahmoud Abd El Baky Rehab, G El Gendy Sherein
African Journal of Microbiology Research.2016; 10(20): 694. CrossRef - The roles of CDR1, CDR2, and MDR1 in kaempferol-induced suppression with fluconazole-resistant Candida albicans
Jing Shao, MengXiang Zhang, TianMing Wang, Yue Li, ChangZhong Wang
Pharmaceutical Biology.2016; 54(6): 984. CrossRef - Potent Synergy between Spirocyclic Pyrrolidinoindolinones and Fluconazole against Candida albicans
Ilandari Dewage Udara Anulal Premachandra, Kevin A. Scott, Chengtian Shen, Fuqiang Wang, Shelley Lane, Haoping Liu, David L. Van Vranken
ChemMedChem.2015; 10(10): 1672. CrossRef - Th17 response and its regulation in inflammatory upper airway diseases
Y. Liu, M. Zeng, Z. Liu
Clinical & Experimental Allergy.2015; 45(3): 602. CrossRef -
Role of Pathogen-Derived Cell Wall Carbohydrates and Prostaglandin E
2
in Immune Response and Suppression of Fish Immunity by the Oomycete Saprolegnia parasitica
Rodrigo Belmonte, Tiehui Wang, Gary J. Duncan, Ida Skaar, Hugo Mélida, Vincent Bulone, Pieter van West, Christopher J. Secombes, J. A. Appleton
Infection and Immunity.2014; 82(11): 4518. CrossRef
Research Support, Non-U.S. Gov't
- Solid State Production of Polygalacturonase and Xylanase by Trichoderma Species Using Cantaloupe and Watermelon Rinds
-
Saleh A. Mohamed , Abdulrahman L. Al-Malki , Jalaluddin A. Khan , Saleh A. Kabli , Saleh M. Al-Garni
-
J. Microbiol. 2013;51(5):605-611. Published online September 14, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3016-x
-
-
47
View
-
0
Download
-
31
Crossref
-
Abstract
-
Different solid state fermentation (SSF) sources were tested such as cantaloupe and watermelon rinds, orange and banana peels, for the production of polygalacturonase (PG) and xylanase (Xyl) by Trichoderma harzianum and Trichoderma virens. The maximum production of both PG and Xyl were obtained by T. harzianum and T. virnes grown on cantaloupe and watermelon rinds, respectively. Time course, moisture content, temperature, pH, supplementation with carbon and nitrogen sources were optimized to achieve the maximum production of both PG and Xyl of T. harzianum and T. virens using cantaloupe and watermelon rinds, respectively. The maximum production of PG and Xyl of T. harzianum and T. virens was recorded at 4–5 days of incubation, 50–66% moisture, temperature 28–35°C and pH 6–7. The influence of supplementary carbon and nitrogen sources was studied. For T. harzianum, lactose enhanced PG activity from 87 to 120 units/g solid, where starch and maltose enhanced Xyl activity from 40 to 55–60 units/g solid for T. virnes. Among the nitrogen sources, ammonium sulphate, ammonium nitrate, yeast extract and urea increased PG activity from 90 to 110–113 units/g solid for T. harzianum. Similarly, ammonium chloride, ammonium sulphate and yeast extract increased Xyl activity from 45 to 55–70 units/g solid for T. virens.
-
Citations
Citations to this article as recorded by

- Fermentation and valorization of watermelon (Citrullus lanatus) rind wastes into livestock feed using Aspergillus niger and Mucor sp.
Ephraim Ekloh, Levi Yafetto
Scientific African.2024; 23: e02035. CrossRef - Investigating process parameters to enhance (hemi)cellulolytic enzymes activity produced by Trichoderma reesei RUT-C30 using deoiled oil palm mesocarp fiber in solid-state fermentation
Ali Abdulkareem Al-Qassab, Mohd Rafein Zakaria, Robiah Yunus, Mohamad Amran Mohd Salleh, Mohd Noriznan Mokhtar
International Journal of Biological Macromolecules.2024; 276: 134030. CrossRef - Detergent-stable amylase production by Paenibacillus lactis strain OPSA3 isolated from soil; optimization by response surface methodology
Emmanuel Tobechukwu Ugwuoji, Tochukwu Nwamaka T. Nwagu, Lewis Iheanacho Ezeogu
Biotechnology Reports.2023; 39: e00808. CrossRef - Release characteristic of bound polyphenols from tea residues insoluble dietary fiber by mixed solid-state fermentation with cellulose degrading strains CZ-6 and CZ-7
Jingyu Si, Jiayan Xie, Bing Zheng, Jianhua Xie, Yi Chen, Chaoran Yang, Nan Sun, Yuting Wang, Xiaobo Hu, Qiang Yu
Food Research International.2023; 173: 113319. CrossRef - Antioxidant-polyphenols of saw palmetto seeds: statistical optimized production and improved functional properties under solid-state fermentation by Trichoderma reesei
Azza M. Abdel-Aty, Amal Z. Barakat, Roqaya I. Bassuiny, Saleh A. Mohamed
Journal of Food Measurement and Characterization.2023; 17(2): 1132. CrossRef - Valorization of banana peel into α-amylase using one factor at a time (OFAT) assisted artificial neural network (ANN) and its partial purification, characterization, and kinetics study
Shyama Prasad Saha, Shubham Ghosh, Deepika Mazumdar, Sarbobhouma Ghosh, Dipanwita Ghosh, Mahima Misti Sarkar, Swarnendu Roy
Food Bioscience.2023; 53: 102533. CrossRef - Enhancement of polyphenolics and antioxidant activities of jambolan (Syzygium cumini) fruit pulp using solid state fermentation by Aspergillus niger and A. flavus
Murugan Rajan, Julianna Ksarla Santana Andrade, Romy Gleyse Chagas Barros, Tamna Joanan Farias Lima Guedes, Narendra Narain
Biocatalysis and Agricultural Biotechnology.2023; 47: 102589. CrossRef - Exploration of the Potential Application of Banana Peel for Its Effective Valorization: A Review
Shreya Bishnoi, Shweta Sharma, Himani Agrawal
Indian Journal of Microbiology.2023; 63(4): 398. CrossRef - Bioprocessing of Horticultural Wastes by Solid-State Fermentation into Value-Added/Innovative Bioproducts: A Review
Aly Farag El Sheikha, Ramesh C. Ray
Food Reviews International.2023; 39(6): 3009. CrossRef - The synergy of dark septate endophytes and organic residue on Isatis indigotica growth and active ingredients accumulation under drought stress
Wanyun Li, Jiaojie Yao, Chao He, Yanfang Ren, Lili Zhao, Xueli He
Industrial Crops and Products.2023; 203: 117147. CrossRef - Xylanase enhanced second-generation bioethanol production through simultaneous saccharification and fermentation
Mohammed A. Al-Ahdal, Ehab M. M. Ali, Othman A. Baothman, Abdulbasit I. I. Al-sieni, Hasan A. Al-Talhi
Biofuels.2023; 14(10): 1009. CrossRef - Degradation potential of different lignocellulosic residues by Trichoderma longibrachiatum and Trichoderma afroharzianum under solid state fermentation
Wanyun Li, Lili Zhao, Xueli He
Process Biochemistry.2022; 112: 6. CrossRef - Biotechnology approach using watermelon rind for optimization of α-amylase enzyme production from Trichoderma virens using response surface methodology under solid-state fermentation
Heidi M. Abdel-Mageed, Amal Z. Barakat, Roqaya I. Bassuiny, Alshaimaa M. Elsayed, Hala A. Salah, Azza M. Abdel-Aty, Saleh A. Mohamed
Folia Microbiologica.2022; 67(2): 253. CrossRef - Exploitation of cantaloupe peels for bacterial cellulose production and functionalization with green synthesized Copper oxide nanoparticles for diverse biological applications
Ahmed K. Saleh, Hamada El-Gendi, Esmail M. El-Fakharany, Medhat E. Owda, Mohamed A. Awad, Elbadawy A. Kamoun
Scientific Reports.2022;[Epub] CrossRef - Improved production of antioxidant-phenolic compounds and certain fungal phenolic-associated enzymes under solid-state fermentation of chia seeds with Trichoderma reesei: response surface methodology-based optimization
Azza M. Abdel-Aty, Amal Z. Barakat, Roqaya I. Bassuiny, Saleh A. Mohamed
Journal of Food Measurement and Characterization.2022; 16(5): 3488. CrossRef - Biochemical properties of cellulolytic and xylanolytic enzymes from Sporotrichum thermophile and their utility in bioethanol production using rice straw
Bijender Singh, Anju Bala, Anu, Alokika, Vinod Kumar, Davender Singh
Preparative Biochemistry & Biotechnology.2022; 52(2): 197. CrossRef - Preparation of a Calcium Alginate-Coated Polypyrrole/Silver Nanocomposite for Site-Specific Immobilization of Polygalacturonase with High Reusability and Enhanced Stability
Yaaser Q. Almulaiky, Sami A. Al-Harbi
Catalysis Letters.2022; 152(1): 28. CrossRef - Solid-state fermentation for enhancing the nutraceutical content of agrifood by-products: Recent advances and its industrial feasibility
Luis O. Cano y Postigo, Daniel A. Jacobo-Velázquez, Daniel Guajardo-Flores, Luis Eduardo Garcia Amezquita, Tomás García-Cayuela
Food Bioscience.2021; 41: 100926. CrossRef - Eco-Green Conversion of Watermelon Peels to Single Cell Oils Using a Unique Oleaginous Fungus: Lichtheimia corymbifera AH13
Amr Hosny Hashem, Mohamed Sayed Hasanin, Ahmed Mohamed Aly Khalil, Waleed Bakry Suleiman
Waste and Biomass Valorization.2020; 11(11): 5721. CrossRef - Immobilization of pectinase on Zr‐treated pumice for fruit juice industry
Selmihan Sahin, Ismail Ozmen
Journal of Food Processing and Preservation.2020;[Epub] CrossRef - Phenolic Content and Antioxidant Activity of Extracts of 12 Melon (Cucumis melo) Peel Powders Prepared from Commercial Melons
Shirin Mal Ganji, Harmit Singh, Mendel Friedman
Journal of Food Science.2019; 84(7): 1943. CrossRef - Effect of stirring on growth and cellulolytic enzymes production by Trichoderma harzianum in a novel bench-scale solid-state fermentation bioreactor
N. Lopez-Ramirez, T. Volke-Sepulveda, I. Gaime-Perraud, G. Saucedo-Castañeda, E. Favela-Torres
Bioresource Technology.2018; 265: 291. CrossRef - The effect of lyophilization and storage time on the survival rate and hydrolytic activity of Trichoderma strains
Monika Grzegorczyk, Anna Kancelista, Wojciech Łaba, Michał Piegza, Danuta Witkowska
Folia Microbiologica.2018; 63(4): 433. CrossRef - Fruit and Vegetable Waste: Bioactive Compounds, Their Extraction, and Possible Utilization
Narashans Alok Sagar, Sunil Pareek, Sunil Sharma, Elhadi M. Yahia, Maria Gloria Lobo
Comprehensive Reviews in Food Science and Food Safety.2018; 17(3): 512. CrossRef - Far infrared radiated energy-proficient rapid one-pot green hydrolysis of waste watermelon peel: optimization and heterogeneous kinetics of glucose synthesis
Swapnendu Chatterjee, Sourav Barman, Rajat Chakraborty
RSC Advances.2016; 6(78): 74278. CrossRef - Valorization of banana peel: a biorefinery approach
Pranav D. Pathak, Sachin A. Mandavgane, Bhaskar D. Kulkarni
Reviews in Chemical Engineering.2016; 32(6): 651. CrossRef - A Novel Non-Cumbersome Approach Towards Biosynthesis of Pectic-Oligosaccharides by Non-Aflatoxigenic Aspergillus sp. Section Flavi Strain EGY1 DSM 101520 through Citrus Pectin Fermentation
Amira M. Embaby, Ramy R. Melika, Ahmed Hussein, Amal H. El-Kamel, Heba S. Marey, Tamás Papp
PLOS ONE.2016; 11(12): e0167981. CrossRef - Multivariate Optimization and Supplementation Strategies for the Simultaneous Production of Amylases, Cellulases, Xylanases, and Proteases by Aspergillus awamori Under Solid-State Fermentation Conditions
Aline Machado de Castro, Leda R. Castilho, Denise Maria Guimarães Freire
Applied Biochemistry and Biotechnology.2015; 175(3): 1588. CrossRef - Xylanase production by endophyticAspergillus nigerusing pentose-rich hydrothermal liquor from sugarcane bagasse
Diogo Robl, Priscila da Silva Delabona, Patrícia dos Santos Costa, Deise Juliana da Silva Lima, Sarita Candida Rabelo, Ida Chapaval Pimentel, Fernanda Büchli, Fabio Marcio Squina, Gabriel Padilla, José Geraldo da Cruz Pradella
Biocatalysis and Biotransformation.2015; 33(3): 175. CrossRef - Saccharification and hydrolytic enzyme production of alkali pre-treated wheat bran by Trichoderma virens under solid state fermentation
Reda M. El-Shishtawy, Saleh A. Mohamed, Abdullah M. Asiri, Abu-bakr M. Gomaa, Ibrahim H. Ibrahim, Hasan A. Al-Talhi
BMC Biotechnology.2015;[Epub] CrossRef - Solid fermentation of wheat bran for hydrolytic enzymes production and saccharification content by a local isolate Bacillus megatherium
Reda M El-Shishtawy, Saleh A Mohamed, Abdullah M Asiri, Abu-bakr M Gomaa, Ibrahim H Ibrahim, Hasan A Al-Talhi
BMC Biotechnology.2014;[Epub] CrossRef
Journal Article
- Comparative Proteomics Analysis of Sarcosine Insoluble Outer Membrane Proteins from Clarithromycin Resistant and Sensitive Strains of Helicobacter pylori
-
Rebecca Smiley , James Bailey , Mahadevan Sethuraman , Norberto Posecion , M. Showkat Ali
-
J. Microbiol. 2013;51(5):612-618. Published online October 31, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3029-5
-
-
45
View
-
0
Download
-
23
Crossref
-
Abstract
-
Helicobacter pylori causes disease manifestations in humans including chronic gastric and peptic ulcers, gastric cancer, and lymphoid tissue lymphoma. Increasing rates of H. pylori clarithromycin resistance has led to higher rates of disease development. Because antibiotic resistance involves modifications of outer membrane proteins (OMP) in other Gram-negative bacteria, this study focuses on identification of H. pylori OMP’s using comparative proteomic analyses of clarithromycin-susceptible and -resistant H. pylori strains. Comparative proteomics analyses of isolated sarcosine-insoluble OMP fractions from clarithromycin-susceptible and -resistant H. pylori strains were performed by 1) one dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis protein separation and 2) in-gel digestion of the isolated proteins and mass spectrometry analysis by Matrix Assisted Laser Desorption Ionization-tandem mass spectrometry. Iron-regulated membrane protein, UreaseB, EF-Tu, and putative OMP were down-regulated; HopT (BabB) transmembrane protein, HofC, and OMP31 were up-regulated in clarithromycin-resistant H. pylori. Western blotting and real time PCR, respectively, validated UreaseB subunit and EF-Tu changes at the protein level, and mRNA expression of HofC and HopT. This limited proteomic study provides evidence that alteration of the outer membrane proteins’ profile may be a novel mechanism involved in clarithromycin resistance in H. pylori.
-
Citations
Citations to this article as recorded by

Research Support, N.I.H., Extramural
- Free Mycolic Acid Accumulation in the Cell Wall of the mce1 Operon Mutant Strain of Mycobacterium tuberculosis
-
Sally A. Cantrell , Michael D. Leavell , Olivera Marjanovic , Anthony T. Iavarone , Julie A. Leary , Lee W. Riley
-
J. Microbiol. 2013;51(5):619-626. Published online September 14, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3092-y
-
-
48
View
-
0
Download
-
43
Crossref
-
Abstract
-
The lipid-rich cell wall of Mycobacterium tuberculosis, the agent of tuberculosis, serves as an effective barrier against many chemotherapeutic agents and toxic host cell effector molecules, and it may contribute to the mechanism of persistence. Mycobacterium tuberculosis strains mutated in a 13-gene operon called mce1, which encodes a putative ABC lipid transporter, induce aberrant granulomatous response in mouse lungs. Because of the postulated role of the mce1 operon in lipid importation, we compared the cell wall lipid composition of wild type and mce1 operon mutant M. tuberculosis H37Rv strains. High resolution mass spectrometric analyses of the mce1 mutant lipid extracts showed unbound mycolic acids to accumulate in the cell wall. Quantitative analysis revealed a 10.7 fold greater amount of free mycolates in the mutant compared to that of the wild type strain. The free mycolates were comprised of alpha, methoxy and keto mycolates in the ratio 1:0.9:0.6, respectively. Since the mce1 operon is regulated in vivo, the free mycolates that accumulate during infection may serve as a barrier for M. tuberculosis against toxic products and contribute to the pathogen’s persistence.
-
Citations
Citations to this article as recorded by

- Antibiotic resistance in Mycobacterium tuberculosis alters tolerance to cell wall-targeting inhibitors
William J Jowsey, Gregory M Cook, Matthew B McNeil
JAC-Antimicrobial Resistance.2024;[Epub] CrossRef - Genomic analysis of Mycobacterium brumae sustains its nonpathogenic and immunogenic phenotype
Chantal Renau-Mínguez, Paula Herrero-Abadía, Paula Ruiz-Rodriguez, Vicente Sentandreu, Eduard Torrents, Álvaro Chiner-Oms, Manuela Torres-Puente, Iñaki Comas, Esther Julián, Mireia Coscolla
Frontiers in Microbiology.2023;[Epub] CrossRef - Mycobacterial mycolic acids trigger inhibitory receptor Clec12A to suppress host immune responses
Naoya Nishimura, Noriyuki Tomiyasu, Shota Torigoe, Satoru Mizuno, Hanako Fukano, Eri Ishikawa, Harutaka Katano, Yoshihiko Hoshino, Kazuhiro Matsuo, Masatomo Takahashi, Yoshihiro Izumi, Takeshi Bamba, Koichi Akashi, Sho Yamasaki
Tuberculosis.2023; 138: 102294. CrossRef - Mce1R of Mycobacterium tuberculosis prefers long-chain fatty acids as specific ligands: a computational study
Dipanwita Maity, Dheeraj Singh, Amitava Bandhu
Molecular Diversity.2023; 27(6): 2523. CrossRef - Structure of an endogenous mycobacterial MCE lipid transporter
James Chen, Alice Fruhauf, Catherine Fan, Jackeline Ponce, Beatrix Ueberheide, Gira Bhabha, Damian C. Ekiert
Nature.2023; 620(7973): 445. CrossRef - Protein target highlights in CASP15: Analysis of models by structure providers
Leila T. Alexander, Janani Durairaj, Andriy Kryshtafovych, Luciano A. Abriata, Yusupha Bayo, Gira Bhabha, Cécile Breyton, Simon G. Caulton, James Chen, Séraphine Degroux, Damian C. Ekiert, Benedikte S. Erlandsen, Peter L. Freddolino, Dominic Gilzer, Chris
Proteins: Structure, Function, and Bioinformatics.2023; 91(12): 1571. CrossRef - Role of Ring6 in the Function of the E. coli MCE Protein LetB
Casey Vieni, Nicolas Coudray, Georgia L. Isom, Gira Bhabha, Damian C. Ekiert
Journal of Molecular Biology.2022; 434(7): 167463. CrossRef - Mycobacterial MCE proteins as transporters that control lipid homeostasis of the cell wall
Laura I. Klepp, Julia Sabio y Garcia, FabianaBigi
Tuberculosis.2022; 132: 102162. CrossRef - Transporters Involved in the Biogenesis and Functionalization of the Mycobacterial Cell Envelope
Mary Jackson, Casey M. Stevens, Lei Zhang, Helen I. Zgurskaya, Michael Niederweis
Chemical Reviews.2021; 121(9): 5124. CrossRef - Trehalose Recycling Promotes Energy-Efficient Biosynthesis of the Mycobacterial Cell Envelope
Amol Arunrao Pohane, Caleb R. Carr, Jaishree Garhyan, Benjamin M. Swarts, M. Sloan Siegrist, Graham F. Hatfull
mBio.2021;[Epub] CrossRef - TREM2 is a receptor for non-glycosylated mycolic acids of mycobacteria that limits anti-mycobacterial macrophage activation
Ei’ichi Iizasa, Yasushi Chuma, Takayuki Uematsu, Mio Kubota, Hiroaki Kawaguchi, Masayuki Umemura, Kenji Toyonaga, Hideyasu Kiyohara, Ikuya Yano, Marco Colonna, Masahiko Sugita, Goro Matsuzaki, Sho Yamasaki, Hiroki Yoshida, Hiromitsu Hara
Nature Communications.2021;[Epub] CrossRef - Pan‐genomic analysis reveals that the evolution of Dietzia species depends on their living habitats
Hui Fang, Jin‐Bo Xu, Yong Nie, Xiao‐Lei Wu
Environmental Microbiology.2021; 23(2): 861. CrossRef - Molecular Cloning, Purification and Characterization of Mce1R of Mycobacterium tuberculosis
Dipanwita Maity, Rajasekhara Reddy Katreddy, Amitava Bandhu
Molecular Biotechnology.2021; 63(3): 200. CrossRef - Synthesis and recycling of the mycobacterial cell envelope
Katherine A Abrahams, Gurdyal S Besra
Current Opinion in Microbiology.2021; 60: 58. CrossRef - Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis
Pooja Asthana, Dhirendra Singh, Jan Skov Pedersen, Mikko J. Hynönen, Ramita Sulu, Abhinandan V. Murthy, Mikko Laitaoja, Janne Jänis, Lee W. Riley, Rajaram Venkatesan
IUCrJ.2021; 8(5): 757. CrossRef - Mycobacterium abscessus biofilms produce an extracellular matrix and have a distinct mycolic acid profile
Anja Dokic, Eliza Peterson, Mario L. Arrieta-Ortiz, Min Pan, Alessandro Di Maio, Nitin Baliga, Apoorva Bhatt
The Cell Surface.2021; 7: 100051. CrossRef - ATP disrupts lipid-binding equilibrium to drive retrograde transport critical for bacterial outer membrane asymmetry
Wen-Yi Low, Shuhua Thong, Shu-Sin Chng
Proceedings of the National Academy of Sciences.2021;[Epub] CrossRef - High-Throughput Screen for Cell Wall Synthesis Network Module in Mycobacterium tuberculosis Based on Integrated Bioinformatics Strategy
Xizi Luo, Jiahui Pan, Qingyu Meng, Juanjuan Huang, Wenfang Wang, Nan Zhang, Guoqing Wang
Frontiers in Bioengineering and Biotechnology.2020;[Epub] CrossRef - LetB Structure Reveals a Tunnel for Lipid Transport across the Bacterial Envelope
Georgia L. Isom, Nicolas Coudray, Mark R. MacRae, Collin T. McManus, Damian C. Ekiert, Gira Bhabha
Cell.2020; 181(3): 653. CrossRef - Differential Host Pro-Inflammatory Response to Mycobacterial Cell Wall Lipids Regulated by the Mce1 Operon
Jéssica D. Petrilli, Igor Müller, Luana E. Araújo, Thiago M. Cardoso, Lucas P. Carvalho, Bruna C. Barros, Maurício Teixeira, Sérgio Arruda, Lee W. Riley, Adriano Queiroz
Frontiers in Immunology.2020;[Epub] CrossRef - Rise of Clinical Microbial Proteogenomics: A Multiomics Approach to Nontuberculous Mycobacterium—The Case ofMycobacterium abscessusUC22
Jayshree Advani, Renu Verma, Oishi Chatterjee, Rex Devasahayam Arokia Balaya, Mohd Altaf Najar, Namitha Ravishankara, Sneha Suresh, Praveen Kumar Pachori, Umesh D. Gupta, Sneha M. Pinto, Devendra S. Chauhan, Srikanth Prasad Tripathy, Harsha Gowda, T.S. Ke
OMICS: A Journal of Integrative Biology.2019; 23(1): 1. CrossRef - Mce-associated protein Rv0177 alters the cell wall structure of Mycobacterium smegmatis and promotes macrophage apoptosis via regulating the cytokines
Shuangquan Yan, Junfeng Zhen, Yue Li, Chenhui Zhang, Andrea Stojkoska, Nzungize Lambert, Qiming Li, Ping Li, Jianping Xie
International Immunopharmacology.2019; 66: 205. CrossRef - Microenvironment ofMycobacterium smegmatisCulture to Induce Cholesterol Consumption Does Cell Wall Remodeling and Enables the Formation of Granuloma-Like Structures
Ana Cristina Doria dos Santos, Victor Hugo de Souza Marinho, Pedro Henrique de Aviz Silva, Barbarella de Matos Macchi, Mara Silvia Pinheiro Arruda, Edilene Oliveira da Silva, José Luiz Martins do Nascimento, Chubert Bernardo Castro de Sena
BioMed Research International.2019; 2019: 1. CrossRef - A highly selective fluorescent probe for real-time imaging of bacterial NAT2 and high-throughput screening of natural inhibitors for tuberculosis therapy
Yinzhu Jin, Zhenhao Tian, Xiangge Tian, Lei Feng, Jingnan Cui, Xiaochi Ma
Materials Chemistry Frontiers.2019; 3(1): 145. CrossRef - Cholesterol and fatty acids grease the wheels of Mycobacterium tuberculosis pathogenesis
Kaley M Wilburn, Rachael A Fieweger, Brian C VanderVen
Pathogens and Disease.2018;[Epub] CrossRef - Mce2R/Rv0586 of Mycobacterium tuberculosis is the functional homologue of FadR E. coli
Suhail Yousuf, Rajendra Kumar Angara, Ajit Roy, Shailesh Kumar Gupta, Rohan Misra, Akash Ranjan
Microbiology.2018; 164(9): 1133. CrossRef - Cell envelope stress in mycobacteria is regulated by the novel signal transduction ATPase IniR in response to trehalose
Maikel Boot, Vincent J. C. van Winden, Marion Sparrius, Robert van de Weerd, Alexander Speer, Roy Ummels, Tige Rustad, David R. Sherman, Wilbert Bitter, Carmen Buchrieser
PLOS Genetics.2017; 13(12): e1007131. CrossRef - “Genetic regulation of Mycobacterium tuberculosis in a lipid-rich environment”
Diana A. Aguilar-Ayala, Juan Carlos Palomino, Peter Vandamme, Anandi Martin, Jorge A. Gonzalez-y-Merchand
Infection, Genetics and Evolution.2017; 55: 392. CrossRef - MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis
Georgia L. Isom, Nathaniel J. Davies, Zhi-Soon Chong, Jack A. Bryant, Mohammed Jamshad, Maria Sharif, Adam F. Cunningham, Timothy J. Knowles, Shu-Sin Chng, Jeffrey A. Cole, Ian R. Henderson
Scientific Reports.2017;[Epub] CrossRef - Bacterial immunostat: Mycobacterium tuberculosis lipids and their role in the host immune response
Adriano Queiroz, Lee W. Riley
Revista da Sociedade Brasileira de Medicina Tropical.2017; 50(1): 9. CrossRef -
The Sec Pathways and Exportomes of
Mycobacterium tuberculosis
Brittany K. Miller, Katelyn E. Zulauf, Miriam Braunstein, William R. Jacobs Jr., Helen McShane, Valerie Mizrahi, Ian M. Orme
Microbiology Spectrum.2017;[Epub] CrossRef - Rv3723/LucA coordinates fatty acid and cholesterol uptake in Mycobacterium tuberculosis
Evgeniya V Nazarova, Christine R Montague, Thuy La, Kaley M Wilburn, Neelima Sukumar, Wonsik Lee, Shannon Caldwell, David G Russell, Brian C VanderVen
eLife.2017;[Epub] CrossRef - The transcriptome of Mycobacterium tuberculosis in a lipid-rich dormancy model through RNAseq analysis
Diana A. Aguilar-Ayala, Laurentijn Tilleman, Filip Van Nieuwerburgh, Dieter Deforce, Juan Carlos Palomino, Peter Vandamme, Jorge A. Gonzalez-Y-Merchand, Anandi Martin
Scientific Reports.2017;[Epub] CrossRef - Transcriptional Profiling of Mycobacterium tuberculosis Exposed toIn VitroLysosomal Stress
Wenwei Lin, Paola Florez de Sessions, Garrett Hor Keong Teoh, Ahmad Naim Nazri Mohamed, Yuan O. Zhu, Vanessa Hui Qi Koh, Michelle Lay Teng Ang, Peter C. Dedon, Martin Lloyd Hibberd, Sylvie Alonso, S. Ehrt
Infection and Immunity.2016; 84(9): 2505. CrossRef - Metabolic profile of Mycobacterium smegmatis reveals Mce4 proteins are relevant for cell wall lipid homeostasis
María Paz Santangelo, Adam Heuberger, Federico Blanco, Marina Forrellad, Catalina Taibo, Laura Klepp, Julia Sabio García, Pablo I. Nikel, Mary Jackson, Fabiana Bigi
Metabolomics.2016;[Epub] CrossRef - An orphaned Mce‐associated membrane protein of Mycobacterium tuberculosis is a virulence factor that stabilizes Mce transporters
Ellen Foot Perkowski, Brittany K. Miller, Jessica R. McCann, Jonathan Tabb Sullivan, Seidu Malik, Irving Coy Allen, Virginia Godfrey, Jennifer D. Hayden, Miriam Braunstein
Molecular Microbiology.2016; 100(1): 90. CrossRef - Standardization of natural mycolic acid antigen composition and production for use in biomarker antibody detection to diagnose active tuberculosis
F.L. Ndlandla, V. Ejoh, A.C. Stoltz, B. Naicker, A.D. Cromarty, S. van Wyngaardt, M. Khati, L.S. Rotherham, Y. Lemmer, J. Niebuhr, C.R. Baumeister, J.R. Al Dulayymi, H. Swai, M.S. Baird, J.A. Verschoor
Journal of Immunological Methods.2016; 435: 50. CrossRef - Screening of the antimycobacterial activity of novel lipophilic agents by the modified broth based method
Mehdi Zandhaghighi, Kiarash Ghazvini, Zahra Meshkat, Seyed Abdolrahim Rezaee, Mohammad Derakhshan, Saman Soleimanpour, Farzin Hadizadeh
Journal of Clinical Tuberculosis and Other Mycobacterial Diseases.2016; 3: 1. CrossRef - Comparative metabolic profiling ofmce1operon mutant vs wild-typeMycobacterium tuberculosisstrains
Adriano Queiroz, Daniel Medina-Cleghorn, Olivera Marjanovic, Daniel K. Nomura, Lee W. Riley, Patricia Bozza
Pathogens and Disease.2015; 73(8): ftv066. CrossRef - Mycobacterium tuberculosis effectors involved in host–pathogen interaction revealed by a multiple scales integrative pipeline
Wu Li, Xiangyu Fan, Quanxin Long, Longxiang Xie, Jianping Xie
Infection, Genetics and Evolution.2015; 32: 1. CrossRef - Label-free Quantitative Proteomics Reveals a Role for the Mycobacterium tuberculosis SecA2 Pathway in Exporting Solute Binding Proteins and Mce Transporters to the Cell Wall*
Meghan E. Feltcher, Harsha P. Gunawardena, Katelyn E. Zulauf, Seidu Malik, Jennifer E. Griffin, Christopher M. Sassetti, Xian Chen, Miriam Braunstein
Molecular & Cellular Proteomics.2015; 14(6): 1501. CrossRef - Role of host- and pathogen-associated lipids in directing the immune response in mycobacterial infections, with emphasis onMycobacterium aviumsubsp.paratuberculosis
Shyamala Thirunavukkarasu, Kumudika de Silva, Karren M. Plain, Richard J. Whittington
Critical Reviews in Microbiology.2014; : 1. CrossRef - Inhibition of toll-like receptor 2 (TLR-2)-mediated response in human alveolar epithelial cells by mycolic acids andMycobacterium tuberculosismce1operon mutant
Patricia C. Sequeira, Ryan H. Senaratne, Lee W. Riley
Pathogens and Disease.2014; 70(2): 132. CrossRef
Research Support, Non-U.S. Gov'ts
- Crystal Structure of XoLAP, a Leucine Aminopeptidase, from Xanthomonas oryzae pv. oryzae
-
Jin-Kwang Kim , Sampath Natarajan , Hanseul Park , Kim-Hung Huynh , Sang Hee Lee , Jeong-Gu Kim , Yeh-Jin Ahn , Lin-Woo Kang
-
J. Microbiol. 2013;51(5):627-632. Published online October 31, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3234-2
-
-
46
View
-
0
Download
-
7
Crossref
-
Abstract
-
Aminopeptidases are metalloproteinases that degrade N-terminal residues from protein and play important roles in cell growth and development by controlling cell homeostasis and protein maturation. We determined the crystal structure of XoLAP, a leucyl aminopeptidase, at 2.6 Å resolution from Xanthomonas oryzae pv. oryzae, causing the destructive rice disease of bacterial blight. It is the first crystal structure of aminopeptidase from phytopathogens as a drug target. XoLAP existed as a hexamer and the monomer structure consisted of an N-terminal cap domain and a C-terminal peptidase domain with two divalent zinc ions. XoLAP structure was compared with BlLAP and EcLAP (EcPepA) structures. Based on the structural comparison, the molecular model of XoLAP in complex with the natural aminopeptidase inhibitor of microginin FR1 was proposed. The model structure will be useful to develop a novel antibacterial drug against Xoo.
-
Citations
Citations to this article as recorded by

- Expression, Characterisation, Homology Modelling and Molecular Docking of a Novel M17 Family Leucyl-Aminopeptidase from Bacillus cereus CZ
Jie Liu, Tangbing Cui
International Journal of Molecular Sciences.2023; 24(21): 15939. CrossRef - Screening and verification for proteins that interact with leucine aminopeptidase of Taenia pisiformis using a yeast two-hybrid system
Shaohua Zhang
Parasitology Research.2019; 118(12): 3387. CrossRef - Transcriptional expression of aminoacyl tRNA synthetase genes of Xanthomonas oryzae pv. oryzae (Xoo) on rice-leaf extract treatment and crystal structure of Xoo glutamyl-tRNA synthetase
Thien-Hoang Ho, Myoung-Ki Hong, Seunghwan Kim, Jeong-Gu Kim, Jongha Lee, Kyoungho Jung, Inho Lee, Munyoung Choi, Hyunjae Park, Sanghee Lee, Yeh-Jin Ahn, Lin-Woo Kang
Crop and Pasture Science.2017; 68(5): 434. CrossRef - An angiotensin-converting enzyme-inhibitory metabolite with partial structure of microginin in a cyanobacterium Anabaena fertilissima CCC597, producing fibrinolytic protease
Suvendra Nath Bagchi, Shobha Sondhia, Manish Kumar Agrawal, Sonali Banerjee
Journal of Applied Phycology.2016; 28(1): 177. CrossRef - Structure and Substrate Recognition of the Bottromycin Maturation Enzyme BotP
Greg Mann, Liujie Huo, Sebastian Adam, Brunello Nardone, Jeremie Vendome, Nicholas James Westwood, Rolf Müller, Jesko Koehnke
ChemBioChem.2016; 17(23): 2286. CrossRef - Crystal Structures of Peptide Deformylase from Rice Pathogen Xanthomonas oryzae pv. oryzae in Complex with Substrate Peptides, Actinonin, and Fragment Chemical Compounds
Ho-Phuong-Thuy Ngo, Thien-Hoang Ho, Inho Lee, Huyen-Thi Tran, Bookyo Sur, Seunghwan Kim, Jeong-Gu Kim, Yeh-Jin Ahn, Sun-Shin Cha, Lin-Woo Kang
Journal of Agricultural and Food Chemistry.2016; 64(39): 7307. CrossRef - Crystallization and preliminary X-ray crystallographic analysis of the XoGroEL chaperonin fromXanthomonas oryzaepv.oryzae
Huyen-Thi Tran, Tan-Viet Pham, Ho-Phuong-Thuy Ngo, Myoung-Ki Hong, Jeong-Gu Kim, Sang Hee Lee, Yeh-Jin Ahn, Lin-Woo Kang
Acta Crystallographica Section F Structural Biology Communications.2014; 70(5): 604. CrossRef
- Safety Evaluation of Lactobacillus paracasei subsp. paracasei LC-01, Probiotic Bacterium
-
Hao Zhang , Yu Wang , Jing Sun , Zirui Guo , Huiyuan Guo , Fazheng Ren
-
J. Microbiol. 2013;51(5):633-638. Published online October 31, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3336-x
-
-
45
View
-
0
Download
-
9
Crossref
-
Abstract
-
The safety of Lactobacillus paracasei subsp. paracasei LC-01 was evaluated for its use as a potential probiotic. In our in vitro study, the antibiotic resistance and the ability to produce biogenic amine were determined. The results showed that the strain was sensitive to all tested antibiotics and did not produce biogenic amine except for tyramine. The oral toxicity of this strain was evaluated in Balb/C mice. One hundred mice were divided into 10 groups. Four groups were administered 0, 108, 109, or 1010 CFU/mouse per day dissolved in saline solution respectively, for 28 days. Three groups were injected intraperitoneally with 109 CFU/mouse dissolved in saline solution, and were killed 2, 5, and 10 days after injection. The last 3 groups were injected with the vehicle as controls respectively. The results showed that oral administration of the strain had no adverse effects on mouse body weight and that there was no treatment-associated bacterial translocation. Intraperitoneal administration caused a significant translocation to liver, spleen and kidney. However, this translocation did not cause illness or death throughout the experiment. The results suggest that L. paracasei subsp. paracasei LC-01 is likely to be safe for human consumption.
-
Citations
Citations to this article as recorded by

- Genome sequence and evaluation of safety and probiotic potential of Lacticaseibacillus paracasei LC86 and Lacticaseibacillus casei LC89
Ting Chen, Yunjiao Zhao, Yixuan Fan, Yao Dong, Zhonghui Gai
Frontiers in Microbiology.2025;[Epub] CrossRef - Lactobacillus paracasei R3 Alleviates Tumor Progression in Mice with Colorectal Cancer
Tao Chen, Baoxia Li, Kangdi Zheng, Yan Liu, Zhao Zhang, Huimei Hu, Guoqiang Qian, Jianwei Jiang
Current Microbiology.2024;[Epub] CrossRef - Comprehensive genomic analysis and evaluation of in vivo and in vitro safety of Heyndrickxia coagulans BC99
Ying Wu, Zhiyi Wu, Yinyin Gao, Yixuan Fan, Yao Dong, Yinan Zhang, Zhonghui Gai, Shaobin Gu
Scientific Reports.2024;[Epub] CrossRef - Assessment of the Safety and Probiotic Properties of Enterococcus faecium B13 Isolated from Fermented Chili
Jingmin Xiao, Cai Chen, Zhuxian Fu, Shumin Wang, Fan Luo
Microorganisms.2024; 12(5): 994. CrossRef - Lactic acid bacteria with a strong antioxidant function isolated from “Jiangshui,” pickles, and feces
Yue Hu, Yan Zhao, Xu Jia, Dan Liu, Xinhe Huang, Cheng Wang, Yanhua Zhu, Changwu Yue, Shanshan Deng, Yuhong Lyu
Frontiers in Microbiology.2023;[Epub] CrossRef - Assessment of the Safety of Lactobacillus casei IMV B-7280 Probiotic Strain on a Mouse Model
Lazarenko L.M., Babenko L.P., Gichka S.G., Sakhno L.O., Demchenko O.M., Bubnov R.V., Sichel L.M., Spivak M.Ya.
Probiotics and Antimicrobial Proteins.2021; 13(6): 1644. CrossRef - Health benefits and technological effects of Lacticaseibacillus casei-01: An overview of the scientific literature
Tatiana Colombo Pimentel, Larissa Ramalho Brandão, Matthaws Pereira de Oliveira, Whyara Karoline Almeida da Costa, Marciane Magnani
Trends in Food Science & Technology.2021; 114: 722. CrossRef - Lactobacillus casei LC01 Regulates Intestinal Epithelial Permeability through miR-144 Targeting of OCLN and ZO1
Qiuke Hou, Yongquan Huang, Yan Wang, Liu Liao, Zhaoyang Zhu, Wenjie Zhang, Yongshang Liu, Peiwu Li, Xinlin Chen, Fengbin Liu
Journal of Microbiology and Biotechnology.2020; 30(10): 1480. CrossRef - Combinations of cereal β-glucans and probiotics can enhance the anti-inflammatory activity on host cells by a synergistic effect
Mattia Pia Arena, Pasquale Russo, Vittorio Capozzi, Ana Rascón, Giovanna E. Felis, Giuseppe Spano, Daniela Fiocco
Journal of Functional Foods.2016; 23: 12. CrossRef
- Experimental Phasing Using Zinc and Sulfur Anomalous Signals Measured at the Zinc Absorption Peak
-
Sangmin Lee , Min-Kyu Kim , Chang-Jun Ji , Jin-Won Lee , Sun-Shin Cha
-
J. Microbiol. 2013;51(5):639-643. Published online October 31, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3412-2
-
-
Abstract
-
Iron is an essential transition metal required for bacterial growth and survival. Excess free iron can lead to the generation of reactive oxygen species that can cause severe damage to cellular functions. Cells have developed iron-sensing regulators to maintain iron homeostasis at the transcription level. The ferric uptake regulator (Fur) is an iron-responsive regulator that controls the expression of genes involved in iron homeostasis, bacterial virulence, stress resistance, and redox metabolism. Here, we report the expression, purification, crystallization, and phasing of the apo-form of Bacillus subtilis Fur (BsFur) in the absence of regulatory metal ions. Crystals were obtained by microbatch crystallization method at 295 K and diffraction data at a resolution of 2.6 Å was collected at the zinc peak wavelength (λ=1.2823 Å). Experimental phasing identified the positions of one zinc atom and four sulfur atoms of cysteine residues coordinating the zinc atom, indicating that the data contained a meaningful anomalous scattering originating from the ordered zinc-coordinating sulfur atoms, in spite of the small anomalous signals of sulfur atoms at the examined wavelength.
- Involvement of Phosphatidylinositol 3-Kinase/Akt Signaling Pathway in β1 Integrin-Mediated Internalization of Staphylococcus aureus by Alveolar Epithelial Cells
-
Jia-He Wang , Ke Zhang , Nan Wang , Xiao-Min Qiu , Yi-Bing Wang , Ping He
-
J. Microbiol. 2013;51(5):644-650. Published online June 25, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3040-x
-
-
49
View
-
0
Download
-
19
Crossref
-
Abstract
-
The invasion of Staphylococcus aureus into alveolar epithelial cells is regarded as the key step for S. aureus lung infection. However, the mechanism of internalization of S. aureus by alveolar epithelial cells is not clear, and was the aim of this investigation Human lung adenocarcinomic epithelial cells and A549 cells were used. Human β1 integrin and rat β1 integrin were detected by real-time reverse transcription (RT)-PCR. The expressions of β1 integrin, Akt and p-Akt were detected by Western blot analysis. To further investigate the role of β1 integrin in S. aureus internalization by alveolar epithelial cells, we next performed siRNA-mediated knockdown of β1 integrin expression. In this study, we found that S. aureus invades human alveolar epithelial cells and rat primary alveolar epithelial cells. The β1 integrin ligand competitive inhibitor, GRGDS-peptide, blocked the internalization of S. aureus by A549 cells. Knockdown of β1 integrin also inhibited the internalization of S. aureus. In addition, the PI3K/Akt signaling pathway in alveolar epithelial cells was activated by the infection with S. aureus. Furthermore, Akt phosphorylation was abolished by transient transfection with β1 integrin siRNA in A549 cells challenged with S. aureus. Our results suggest that the phosphatidylinositol 3-kinase/Akt signaling pathway plays an important role in β1 integrin-mediated internalization of S. aureus by alveolar epithelial cells.
-
Citations
Citations to this article as recorded by

- Effects of Titanium Dioxide Nanoparticles on Cell Growth and Migration of A549 Cells under Simulated Microgravity
Mei Wang, Jinxia Li, Shunyu Zhang, Yue You, Xianyu Zhu, Huandong Xiang, Liang Yan, Feng Zhao, Yunhui Li
Nanomaterials.2022; 12(11): 1879. CrossRef - Host Response of Human Epidermis to Methicillin-Resistant Staphylococcus aureus Biofilm Infection and Synthetic Antibiofilm Peptide Treatment
Bing (Catherine) Wu, Travis M. Blimkie, Evan F. Haney, Reza Falsafi, Noushin Akhoundsadegh, Robert E. W. Hancock
Cells.2022; 11(21): 3459. CrossRef - Non-Canonical Host Intracellular Niche Links to New Antimicrobial Resistance Mechanism
Michaela Kember, Shannen Grandy, Renee Raudonis, Zhenyu Cheng
Pathogens.2022; 11(2): 220. CrossRef - Identification and Characterization of Staphylococcus delphini Internalization Pathway in Nonprofessional Phagocytic Cells
Yousef Maali, Alan Diot, Patrícia Martins-Simões, Michele Bes, Daniel Bouvard, François Vandenesch, Paul O. Verhoeven, Frederic Laurent, Sophie Trouillet-Assant, Victor J. Torres
Infection and Immunity.2020;[Epub] CrossRef - Study on Intervention Mechanism of Yiqi Huayu Jiedu Decoction on ARDS Based on Network Pharmacology
Xu Liang, Changyong Luo, Yan Li, Xin Li, Qian Wang, Shujing Zhang, Qingqiao Sun, Yuanhong Ma, Caihua Xiong, Yanpeng Zeng, Huu-Tung Nguyen
Evidence-Based Complementary and Alternative Medicine.2020;[Epub] CrossRef - Characterization of the Atl-mediated staphylococcal internalization mechanism
Tim Schlesier, Anke Siegmund, Ursula Rescher, Christine Heilmann
International Journal of Medical Microbiology.2020; 310(8): 151463. CrossRef - Microbial uptake by the respiratory epithelium: outcomes for host and pathogen
Margherita Bertuzzi, Gemma E Hayes, Elaine M Bignell
FEMS Microbiology Reviews.2019; 43(2): 145. CrossRef - Human Bronchial Epithelial Cells Inhibit Aspergillus fumigatus Germination of Extracellular Conidia via FleA Recognition
Nicolas Richard, Léa Marti, Annabelle Varrot, Loïc Guillot, Juliette Guitard, Christophe Hennequin, Anne Imberty, Harriet Corvol, Michel Chignard, Viviane Balloy
Scientific Reports.2018;[Epub] CrossRef - Staphylococcal Adhesion and Host Cell Invasion: Fibronectin-Binding and Other Mechanisms
Jérôme Josse, Frédéric Laurent, Alan Diot
Frontiers in Microbiology.2017;[Epub] CrossRef - α‐Tocopheryl Phosphate Induces VEGF Expression via CD36/PI3Kγ in THP‐1 Monocytes
Jean‐Marc Zingg, Angelo Azzi, Mohsen Meydani
Journal of Cellular Biochemistry.2017; 118(7): 1855. CrossRef - Increased survival and proliferation of the epidemic strain Mycobacterium abscessus subsp. massiliense CRM0019 in alveolar epithelial cells
Giovanni Monteiro Ribeiro, Cristianne Kayoko Matsumoto, Fernando Real, Daniela Teixeira, Rafael Silva Duarte, Renato Arruda Mortara, Sylvia Cardoso Leão, Cristiane de Souza Carvalho-Wodarz
BMC Microbiology.2017;[Epub] CrossRef - Orthopedic implant infections: Incompetence of Staphylococcus epidermidis, Staphylococcus lugdunensis, and Enterococcus faecalis to invade osteoblasts
Davide Campoccia, Francesca Testoni, Stefano Ravaioli, Ilaria Cangini, Alessandra Maso, Pietro Speziale, Lucio Montanaro, Livia Visai, Carla Renata Arciola
Journal of Biomedical Materials Research Part A.2016; 104(3): 788. CrossRef - Association of intracellular Staphylococcus aureus with prognosis in chronic rhinosinusitis
Judy Ou, Amanda Drilling, Deepti Singhal, Neil C.‐W. Tan, Deanna Wallis‐Hill, Sarah Vreugde, Alkis J. Psaltis, Peter‐John Wormald
International Forum of Allergy & Rhinology.2016; 6(8): 792. CrossRef - Interferon‐γ enhances both the anti‐bacterial and the pro‐inflammatory response of human mast cells to Staphylococcus aureus
Emily J. Swindle, Jared M. Brown, Madeleine Rådinger, Frank R. DeLeo, Dean D. Metcalfe
Immunology.2015; 146(3): 470. CrossRef - Attenuation of the Phosphatidylinositol 3-Kinase/Akt Signaling Pathway by Porphyromonas gingivalis Gingipains RgpA, RgpB, and Kgp
Masaaki Nakayama, Tetsuyoshi Inoue, Mariko Naito, Koji Nakayama, Naoya Ohara
Journal of Biological Chemistry.2015; 290(8): 5190. CrossRef - Transcriptional Profile of Mycobacterium tuberculosis Replicating in Type II Alveolar Epithelial Cells
Michelle B. Ryndak, Krishna K. Singh, Zhengyu Peng, Suman Laal, Ludovic Tailleux
PLOS ONE.2015; 10(4): e0123745. CrossRef - The herbal-derived honokiol and magnolol enhances immune response to infection with methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA)
Eun-Jin Choi, Hyung-Ip Kim, Ji-Ae Kim, Soo Youn Jun, Sang Hyeon Kang, Dong June Park, Seok-Jun Son, Younghoon Kim, Ok Sarah Shin
Applied Microbiology and Biotechnology.2015; 99(10): 4387. CrossRef - Nonprofessional Phagocytic Cell Receptors Involved inStaphylococcus aureusInternalization
Nayeli Alva-Murillo, Joel Edmundo López-Meza, Alejandra Ochoa-Zarzosa
BioMed Research International.2014; 2014: 1. CrossRef - Comparative proteome analysis reveals conserved and specific adaptation patterns of Staphylococcus aureus after internalization by different types of human non-professional phagocytic host cells
Kristin Surmann, Stephan Michalik, Petra Hildebrandt, Philipp Gierok, Maren Depke, Lars Brinkmann, Jörg Bernhardt, Manuela G. Salazar, Zhi Sun, David Shteynberg, Ulrike Kusebauch, Robert L. Moritz, Bernd Wollscheid, Michael Lalk, Uwe Völker, Frank Schmi
Frontiers in Microbiology.2014;[Epub] CrossRef