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Volume 59(7); July 2021
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Review
Potential of Bacillus velezensis as a probiotic in animal feed: a review
Fatima Khalid , Anam Khalid , Yuechi Fu , Qian Hu , Yunfang Zheng , Salman Khan , Zaigui Wang
J. Microbiol. 2021;59(7):627-633.   Published online July 1, 2021
DOI: https://doi.org/10.1007/s12275-021-1161-1
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AbstractAbstract
Bacillus velezensis is a plant growth-promoting bacterium that can also inhibit plant pathogens. However, based on its properties, it is emerging as a probiotic in animal feed. This review focuses on the potential characteristics of B. velezensis for use as a probiotic in the animal feed industry. The review was conducted by collecting recently published articles from peer-reviewed journals. Google Scholar and PubMed were used as search engines to access published literature. Based on the information obtained, the data were divided into three groups to discuss the (i) probiotic characteristics of B. velezensis, (ii) probiotic potential for fish, and (iii) the future potential of this species to be developed as a probiotic for the animal feed industry. Different strains of B. velezensis isolated from different sources were found to have the ability to produce antimicrobial compounds and have a beneficial effect on the gut microbiota, with the potential to be a candidate probiotic in the animal feed industry. This review provides valuable information about the characteristics of B. velezensis, which can provide researchers with a better understanding of the use of this species in the animal feed industry.

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Journal Articles
Effect of biostimulation and bioaugmentation on hydrocarbon degradation and detoxification of diesel-contaminated soil: a microcosm study
Patricia Giovanella , Lídia de Azevedo Duarte , Daniela Mayumi Kita , Valéria Maia de Oliveira , Lara Durães Sette
J. Microbiol. 2021;59(7):634-643.   Published online May 15, 2021
DOI: https://doi.org/10.1007/s12275-021-0395-2
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AbstractAbstract
Soil contamination with diesel oil is quite common during processes of transport and storage. Bioremediation is considered a safe, economical, and environmentally friendly approach for contaminated soil treatment. In this context, studies using hydrocarbon bioremediation have focused on total petroleum hydrocarbon (TPH) analysis to assess process effectiveness, while ecotoxicity has been neglected. Thus, this study aimed to select a microbial consortium capable of detoxifying diesel oil and apply this consortium to the bioremediation of soil contaminated with this environmental pollutant through different bioremediation approaches. Gas chromatography (GC-FID) was used to analyze diesel oil degradation, while ecotoxicological bioassays with the bioindicators Artemia sp., Aliivibrio fischeri (Microtox), and Cucumis sativus were used to assess detoxification. After 90 days of bioremediation, we found that the biostimulation and biostimulation/ bioaugmentation approaches showed higher rates of diesel oil degradation in relation to natural attenuation (41.9 and 26.7%, respectively). Phytotoxicity increased in the biostimulation and biostimulation/bioaugmentation treatments during the degradation process, whereas in the Microtox test, the toxicity was the same in these treatments as that in the natural attenuation treatment. In both the phytotoxicity and Microtox tests, bioaugmentation treatment showed lower toxicity. However, compared with natural attenuation, this approach did not show satisfactory hydrocarbon degradation. Based on the microcosm experiments results, we conclude that a broader analysis of the success of bioremediation requires the performance of toxicity bioassays.

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J. Microbiol. 2021;59(7):644-650.   Published online July 1, 2021
DOI: https://doi.org/10.1007/s12275-021-1218-1
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  • 1 Crossref
AbstractAbstract
Livestock pigs and porcine norovirus could be candidate tools for future studies on the geographic isolation of norovirus. In this study, we provide the first evidence for geographic isolation of the host as a determinant of the distribution of subgenotypes of the porcine norovirus genogroup II (GII) genotype 11. Environmental water samples were collected from peri-urban streams and estuaries in South Korea between 2014 and 2020. In total, 488 GII region C sequences of norovirus open reading frame 2 were isolated. A total of 14 genotypes were detected, two of which (GII.11 and GII.18) corresponded to porcine norovirus. Five human norovirus genotypes (GII.2, GII.3, GII.4, GII.6, and GII.17) and one porcine norovirus genotype (GII.11) comprised the subgenotypes. Integrated analysis of seasonal and geographical factors revealed that the possibility of the co-emergence of different GII.11 subgenotypes in the same province was lower than that of human norovirus subgenotypes in the same province. Additional algorithms designed to eliminate potential biases further supported the estimated restricted geographical spread of the GII.11 subgenotypes. Fecal contamination source tracking revealed low detection rates of porcine norovirus in the absence of upstream pig farms. These results suggest that a one-sided viral transmission route, mainly dependent on indirect contact owing to the limited chance of direct contact between geographically separated livestock pig populations, may be responsible for the restricted geographical spread of the GII.11 subgenotypes.

Citations

Citations to this article as recorded by  
  • Swine Norovirus: Past, Present, and Future
    Lara Cavicchio, Andrea Laconi, Alessandra Piccirillo, Maria Serena Beato
    Viruses.2022; 14(3): 537.     CrossRef
The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus
Renfei Lu , Junfang Sun , Yue Qiu , Miaomiao Zhang , Xingfan Xue , Xue Li , Wenhui Yang , Dongsheng Zhou , Lingfei Hu , Yiquan Zhang
J. Microbiol. 2021;59(7):651-657.   Published online June 1, 2021
DOI: https://doi.org/10.1007/s12275-021-0629-3
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AbstractAbstract
Vibrio parahaemolyticus possesses two types of flagella: a single polar flagellum (Pof) for swimming and the peritrichous lateral flagella (Laf) for swarming. Expression of Laf genes has previously been reported to be regulated by the quorum sensing (QS) regulators AphA and OpaR. In the present study, we showed that OpaR, the QS regulator at high cell density (HCD), acted as a negative regulator of swimming motility and the transcription of Pof genes in V. parahaemolyticus. OpaR bound to the promoter-proximal DNA regions of flgAMN, flgMN, and flgBCDEFGHIJ within the Pof gene loci to repress their transcription, whereas it negatively regulates the transcription of flgKL-flaC in an indirect manner. Thus, this work investigated how QS regulated the swimming motility via direct action of its master regulator OpaR on the transcription of Pof genes in V. parahaemolyticus.

Citations

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  • H-NS-Mediated Regulation of Swimming Motility and Polar Flagellar Gene Expression in Vibrio parahaemolyticus
    Yue Zhou, Jingyang Chang, Feng Li, Mei He, Rui Li, Yaqin Hou, Yiquan Zhang, Renfei Lu, Ming Yang
    Current Microbiology.2025;[Epub]     CrossRef
  • GefB, a GGDEF domain-containing protein, affects motility and biofilm formation of Vibrio parahaemolyticus and is regulated by quorum sensing regulators
    Yining Zhou, Jingyang Chang, Miaomiao Zhang, Xue Li, Xi Luo, Wanpeng Li, Zhukang Tian, Nan Zhang, Bin Ni, Yiquan Zhang, Renfei Lu
    Gene.2025; 933: 148968.     CrossRef
  • The effect of environmental calcium on gene expression, biofilm formation and virulence of Vibrio parahaemolyticus
    Xue Li, Jingyang Chang, Miaomiao Zhang, Yining Zhou, Tingting Zhang, Yiquan Zhang, Renfei Lu
    Frontiers in Microbiology.2024;[Epub]     CrossRef
  • VPA0198, a GGDEF domain-containing protein, affects the motility and biofilm formation of Vibrio parahaemolyticus and is regulated by quorum sensing associated regulators
    Yining Zhou, Jingyang Chang, Miaomiao Zhang, Xue Li, Wenhui Yang, Lingfei Hu, Dongsheng Zhou, Bin Ni, Renfei Lu, Yiquan Zhang
    Microbial Pathogenesis.2024; 195: 106882.     CrossRef
  • Antibacterial and anti-virulence potential of plant phenolic compounds against Vibrio parahaemolyticus
    F. Javier Vazquez-Armenta, M. Olivia Aros-Corrales, M. Lizeth Alvarez-Ainza, A. Thalia Bernal-Mercado, J. Fernando Ayala-Zavala, Adrian Ochoa-Leyva, A. Alexis Lopez-Zavala
    F1000Research.2024; 12: 1256.     CrossRef
  • Environmental magnesium ion affects global gene expression, motility, biofilm formation and virulence of Vibrio parahaemolyticus
    Xue Li, Xiaobai Zhang, Miaomiao Zhang, Xi Luo, Tingting Zhang, Xianjin Liu, Renfei Lu, Yiquan Zhang
    Biofilm.2024; 7: 100194.     CrossRef
  • The histone-like nucleoid-structuring protein encoded by the plasmid pMBL6842 regulates both plasmid stability and host physiology of Pseudoalteromonas rubra SCSIO 6842
    Baiyuan Li, Songwei Ni, Yabo Liu, Jianzhong Lin, Xiaoxue Wang
    Microbiological Research.2024; 286: 127817.     CrossRef
  • The Impact of Vp-Porin, an Outer Membrane Protein, on the Biological Characteristics and Virulence of Vibrio Parahaemolyticus
    Jinyuan Che, Qitong Fang, Shaojie Hu, Binghong Liu, Lei Wang, Xiu Fang, Lekang Li, Tuyan Luo, Baolong Bao
    Biology.2024; 13(7): 485.     CrossRef
  • Phenotypic changes and gene expression profiles of Vibrio parahaemolyticus in response to low concentrations of ampicillin
    Xi Luo, Miaomiao Zhang, Yiquan Zhang, Xue Li, Renfei Lu
    The Journal of Antibiotics.2024; 77(12): 823.     CrossRef
  • Bioprospecting and Exploration of Phytochemicals as Quorum Sensing Inhibitors against Cariogenic Dental Biofilm
    S. Arya, R. Usha
    Journal of Pure and Applied Microbiology.2024; 18(1): 100.     CrossRef
  • Identification of an LysR family transcriptional regulator that activates motility and flagellar gene expression in Vibrio parahaemolyticus
    Jingyang Chang, Yining Zhou, Xue Li, Miaomiao Zhang, Yiquan Zhang, Bin Ni, Renfei Lu
    Letters in Applied Microbiology.2024;[Epub]     CrossRef
  • The LuxO-OpaR quorum-sensing cascade differentially controls Vibriophage VP882 lysis-lysogeny decision making in liquid and on surfaces
    Francis J. Santoriello, Bonnie L. Bassler, Ankur B. Dalia
    PLOS Genetics.2024; 20(7): e1011243.     CrossRef
  • Evaluation of Therapeutic Efficiency of Stylicin against Vibrio parahaemolyticus Infection in Shrimp Penaeus vannamei through Comparative Proteomic Approach
    Saranya Chakrapani, Akshaya Panigrahi, Esakkiraj Palanichamy, Sathish Kumar Thangaraj, Naveenkumar Radhakrishnan, Puspamitra Panigrahi, Radhakrishnan Nagarathnam
    Probiotics and Antimicrobial Proteins.2024; 16(1): 76.     CrossRef
  • CalR Inhibits the Swimming Motility and Polar Flagellar Gene Expression in Vibrio parahaemolyticus
    Jingyang Chang, Yining Zhou, Miaomiao Zhang, Xue Li, Nan Zhang, Xi Luo, Bin Ni, Haisheng Wu, Renfei Lu, Yiquan Zhang
    Journal of Microbiology.2024; 62(12): 1125.     CrossRef
  • Quorum sensing: An emerging role for Vibrio infection and host defense
    Hao-Nan Lin, Xian-Hui Huang, Xin-Jun Miao, Wei-Lin Hu, Yong-Liang Lou, Dan-Li Xie
    Infectious Microbes and Diseases.2024;[Epub]     CrossRef
  • QsvR and OpaR coordinately repress biofilm formation by Vibrio parahaemolyticus
    Miaomiao Zhang, Xingfan Xue, Xue Li, Qimin Wu, Tingting Zhang, Wenhui Yang, Lingfei Hu, Dongsheng Zhou, Renfei Lu, Yiquan Zhang
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • Transcriptomic Profiles of Vibrio parahaemolyticus During Biofilm Formation
    Yiquan Zhang, Tingting Zhang, Yue Qiu, Miaomiao Zhang, Xiuhui Lu, Wenhui Yang, Lingfei Hu, Dongsheng Zhou, Bo Gao, Renfei Lu
    Current Microbiology.2023;[Epub]     CrossRef
  • Antibacterial and anti-virulence potential of plant phenolic compounds against Vibrio parahaemolyticus
    F. Javier Vazquez-Armenta, M. Olivia Aros-Corrales, M. Lizeth Alvarez-Ainza, A. Thalia Bernal-Mercado, J. Fernando Ayala-Zavala, Adrian Ochoa-Leyva, A. Alexis Lopez-Zavala
    F1000Research.2023; 12: 1256.     CrossRef
  • Effect of sublethal dose of chloramphenicol on biofilm formation and virulence in Vibrio parahaemolyticus
    Miaomiao Zhang, Liyan Cai, Xi Luo, Xue Li, Tingting Zhang, Fei Wu, Yiquan Zhang, Renfei Lu
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • Quorum sensing and QsvR tightly control the transcription of vpa0607 encoding an active RNase II-type protein in Vibrio parahaemolyticus
    Yiquan Zhang, Xingfan Xue, Fengjun Sun, Xue Li, Miaomiao Zhang, Qimin Wu, Tingting Zhang, Xi Luo, Renfei Lu
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • QsvR represses the transcription of polar flagellum genes in Vibrio parahaemolyticus
    Miaomiao Zhang, Xingfan Xue, Xue Li, Xi Luo, Qimin Wu, Tingting Zhang, Wenhui Yang, Lingfei Hu, Dongsheng Zhou, Renfei Lu, Yiquan Zhang
    Microbial Pathogenesis.2023; 174: 105947.     CrossRef
  • Transcriptomic Analysis of Vibrio parahaemolyticus Underlying the Wrinkly and Smooth Phenotypes
    Qimin Wu, Xue Li, Tingting Zhang, Miaomiao Zhang, Xingfan Xue, Wenhui Yang, Lingfei Hu, Zhe Yin, Dongsheng Zhou, Yuyu Sun, Renfei Lu, Yiquan Zhang, Sébastien P. Faucher
    Microbiology Spectrum.2022;[Epub]     CrossRef
  • Gut microbiota analysis of Blenniidae fishes including an algae-eating fish and clear boundary formation among isolated Vibrio strains
    Masa-aki Yoshida, Takuma Tanabe, Hideo Akiyoshi, Makoto Kawamukai
    Scientific Reports.2022;[Epub]     CrossRef
  • Computationally Designed Anti-LuxP DNA Aptamer Suppressed Flagellar Assembly- and Quorum Sensing-Related Gene Expression in Vibrio parahaemolyticus
    Nur Afiqah Md Yusof, Siti Aisyah Razali, Azyyati Mohd Padzil, Benjamin Yii Chung Lau, Syarul Nataqain Baharum, Nor Azlan Nor Muhammad, Nurul Hanun Ahmad Raston, Chou Min Chong, Natrah Fatin Mohd Ikhsan, Magdalena Lenny Situmorang, Low Chen Fei
    Biology.2022; 11(11): 1600.     CrossRef
  • An Increase of Seawater Temperature Upregulates the Expression of Vibrio parahaemolyticus Virulence Factors Implicated in Adhesion and Biofilm Formation
    Mélanie Billaud, François Seneca, Eric Tambutté, Dorota Czerucka
    Frontiers in Microbiology.2022;[Epub]     CrossRef
  • Characterization of the RpoN regulon reveals the regulation of motility, T6SS2 and metabolism in Vibrio parahaemolyticus
    Dan Gu, Youkun Zhang, Kangru Wang, Mingzhu Li, Xinan Jiao
    Frontiers in Microbiology.2022;[Epub]     CrossRef
Zinc-binding domain mediates pleiotropic functions of Yvh1 in Cryptococcus neoformans
Jae-Hyung Jin , Myung Kyung Choi , Hyun-Soo Cho , Yong-Sun Bahn
J. Microbiol. 2021;59(7):658-665.   Published online July 1, 2021
DOI: https://doi.org/10.1007/s12275-021-1287-1
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AbstractAbstract
Yvh1 is a dual-specificity phosphatase (DUSP) that is evolutionarily conserved in eukaryotes, including yeasts and humans. Yvh1 is involved in the vegetative growth, differentiation, and virulence of animal and plant fungal pathogens. All Yvh1 orthologs have a conserved DUSP catalytic domain at the N-terminus and a zinc-binding (ZB) domain with two zinc fingers (ZFs) at the C-terminus. Although the DUSP domain is implicated in the regulation of MAPK signaling in humans, only the ZB domain is essential for most cellular functions of Yvh1 in fungi. This study aimed to analyze the functions of the DUSP and ZB domains of Yvh1 in the human fungal pathogen Cryptococcus neoformans, whose Yvh1 (CnYvh1) contains a DUSP domain at the C-terminus and a ZB domain at the N-terminus. Notably, CnYvh1 has an extended internal domain between the two ZF motifs in the ZB domain. To elucidate the function of each domain, we constructed individual domain deletions and swapping strains by complementing the yvh1Δ mutant with wild-type (WT) or mutated YVH1 alleles and examined their Yvh1-dependent phenotypes, including growth under varying stress conditions, mating, and virulence factor production. Here, we found that the complementation of the yvh1Δ mutant with the mutated YVH1 alleles having two ZFs of the ZB domain, but not the DUSP and extended internal domains, restored the WT phenotypic traits in the yvh1Δ mutant. In conclusion, the ZB domain, but not the N-terminal DUSP domain, plays a pivotal role in the pathobiological functions of cryptococcal Yvh1.
The inner membrane protein LapB is required for adaptation to cold stress in an LpxC-independent manner
Han Byeol Lee , Si Hyoung Park , Chang-Ro Lee
J. Microbiol. 2021;59(7):666-674.   Published online May 15, 2021
DOI: https://doi.org/10.1007/s12275-021-1130-8
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AbstractAbstract
The inner membrane protein lipopolysaccharide assembly protein B (LapB) is an adaptor protein that activates the proteolysis of LpxC by an essential inner membrane metalloprotease, FtsH, leading to a decrease in the level of lipopolysaccharide in the membrane. In this study, we revealed the mechanism by which the essential inner membrane protein YejM regulates LapB and analyzed the role of the transmembrane domain of LapB in Escherichia coli. The transmembrane domain of YejM genetically and physically interacted with LapB and inhibited its function, which led to the accumulation of LpxC. The transmembrane domain of LapB was indispensable for both its physical interaction with YejM and its regulation of LpxC proteolysis. Notably, we found that the lapB mutant exhibited strong cold sensitivity and this phenotype was not associated with increased accumulation of LpxC. The transmembrane domain of LapB was also required for its role in adaptation to cold stress. Taken together, these
results
showed that LapB plays an important role in both the regulation of LpxC level, which is controlled by its interaction with the transmembrane domain of YejM, and adaptation to cold stress, which is independent of LpxC.

Citations

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  • PhoPQ-mediated lipopolysaccharide modification governs intrinsic resistance to tetracycline and glycylcycline antibiotics in Escherichia coli
    Byoung Jun Choi, Umji Choi, Dae-Beom Ryu, Chang-Ro Lee, Mehrad Hamidian, You-Hee Cho
    mSystems.2024;[Epub]     CrossRef
  • Lytic transglycosylase repertoire diversity enables intrinsic antibiotic resistance and daughter cell separation in Escherichia coli under acidic stress
    Ji Eun Son, Si Hyoung Park, Umji Choi, Chang-Ro Lee, Laurent Poirel
    Antimicrobial Agents and Chemotherapy.2024;[Epub]     CrossRef
  • Trans-cinnamaldehyde inhibits Escherichia coli growth by regulating lipopolysaccharide accumulation
    Huanling Xing, Xiaomin Liu, Jianhao Lin, Mingfei Sun, Junyi Huang, Xinghai Li, Yanqun Li, Shining Guo, Fang Zhou, Hong Wu
    Food Bioscience.2024; 61: 104559.     CrossRef
  • Coordinated and Distinct Roles of Peptidoglycan Carboxypeptidases DacC and DacA in Cell Growth and Shape Maintenance under Stress Conditions
    Umji Choi, Si Hyoung Park, Han Byeol Lee, Ji Eun Son, Chang-Ro Lee, Cristina Solano
    Microbiology Spectrum.2023;[Epub]     CrossRef
  • NoiD, a DedA membrane protein required for homeostasis maintaining of Rhizobium leguminosarum biovar viciae during symbiosis with Pisum sativum
    Xiaofang Li, Jiaming Xu, Yajuan Wei, Zirui Chen
    Symbiosis.2022; 86(1): 81.     CrossRef
  • Conserved Tandem Arginines for PbgA/YejM Allow Salmonella Typhimurium To Regulate LpxC and Control Lipopolysaccharide Biogenesis during Infection
    Nicole P. Giordano, Joshua A. Mettlach, Zachary D. Dalebroux, Manuela Raffatellu
    Infection and Immunity.2022;[Epub]     CrossRef
  • Divergent Effects of Peptidoglycan Carboxypeptidase DacA on Intrinsic β-Lactam and Vancomycin Resistance
    Si Hyoung Park, Umji Choi, Su-Hyun Ryu, Han Byeol Lee, Jin-Won Lee, Chang-Ro Lee, Krisztina M. Papp-Wallace
    Microbiology Spectrum.2022;[Epub]     CrossRef
  • Cryo-EM structure of transmembrane AAA+ protease FtsH in the ADP state
    Wu Liu, Martien Schoonen, Tong Wang, Sean McSweeney, Qun Liu
    Communications Biology.2022;[Epub]     CrossRef
  • Checkpoints That Regulate Balanced Biosynthesis of Lipopolysaccharide and Its Essentiality in Escherichia coli
    Gracjana Klein, Alicja Wieczorek, Martyna Szuster, Satish Raina
    International Journal of Molecular Sciences.2021; 23(1): 189.     CrossRef
Isolation of a novel strain, Sphingorhabdus sp. YGSMI21 and characterization of its enantioselective epoxide hydrolase activity
Jung-Hee Woo , Hae-Seon Kim , Nyun-Ho Park , Ho Young Suk
J. Microbiol. 2021;59(7):675-680.   Published online June 1, 2021
DOI: https://doi.org/10.1007/s12275-021-1023-x
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AbstractAbstract
Sphingorhabdus sp. YGSMI21, a novel microbial strain with an enantioselective epoxide hydrolase activity, was isolated from tidal samples contaminated by accidental oil spills subjected to enriched culture with polycyclic aromatic hydrocarbon. This strain was able to optically decompose (R)-styrene oxide (SO) and showed 100% optical purity. In addition, it showed a good enantioselectivity for the derivatives of (S)- SO, (S)-2-chlorostyrene oxide (CSO), (S)-3-CSO and (S)-4- CSO. For (S)-2-CSO, (S)-3-CSO and (S)-4-CSO, 99.9%ee was obtained with the yield of 26.2%, 24.8%, and 11.0%, respectively, when using 10 mg cells of Sphingorhabdus sp. YGSMI21 at pH 8.0 with 4 mM racemic substrates at pH 8.0 and 25°C. The values obtained in this study for (S)-2-CSO, particularly the yield of 26.2%, is noteworthy, considering that obtaining an enantiomerically pure form is difficult. Taken together, Sphingorhabdus sp. YGSMI21 can be regarded as a wholecell biocatalyst in the production of various (S)-CSO with the chlorine group at a different position.

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  • Epoxide Hydrolases: Multipotential Biocatalysts
    Marek Bučko, Katarína Kaniaková, Helena Hronská, Peter Gemeiner, Michal Rosenberg
    International Journal of Molecular Sciences.2023; 24(8): 7334.     CrossRef
  • Effects of submerged macrophytes (Elodea nuttallii) on water quality and microbial communities of largemouth bass (Micropterus salmoides) ponds
    Zhijuan Nie, Zhaowei Zheng, Haojun Zhu, Yi Sun, Jun Gao, Jiancao Gao, Pao Xu, Gangchuan Xu
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • Description of Polaribacter batillariae sp. nov., Polaribacter cellanae sp. nov., and Polaribacter pectinis sp. nov., novel bacteria isolated from the gut of three types of South Korean shellfish
    Su-Won Jeong, Jeong Eun Han, June-Young Lee, Ji-Ho Yoo, Do-Yeon Kim, In Chul Jeong, Jee-Won Choi, Yun-Seok Jeong, Jae-Yun Lee, So-Yeon Lee, Euon Jung Tak, Hojun Sung, Hyun Sik Kim, Pil Soo Kim, Dong-Wook Hyun, Jin-Woo Bae
    Journal of Microbiology.2022; 60(6): 576.     CrossRef
Mst1/2-ALK promotes NLRP3 inflammasome activation and cell apoptosis during Listeria monocytogenes infection
Aijiao Gao , Huixin Tang , Qian Zhang , Ruiqing Liu , Lin Wang , Yashan Liu , Zhi Qi , Yanna Shen
J. Microbiol. 2021;59(7):681-692.   Published online April 20, 2021
DOI: https://doi.org/10.1007/s12275-021-0638-2
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AbstractAbstract
Listeria monocytogenes (L. monocytogenes) is a Gram-positive intracellular foodborne pathogen that causes severe diseases, such as meningitis and sepsis. The NLR family pyrin domain-containing 3 (NLRP3) inflammasome has been reported to participate in host defense against pathogen infection. However, the exact molecular mechanisms underlying NLRP3 inflammasome activation remain to be fully elucidated. In the present study, the roles of mammalian Ste20- like kinases 1/2 (Mst1/2) and Anaplastic Lymphoma Kinase (ALK) in the activation of the NLRP3 inflammasome induced by L. monocytogenes infection were investigated. The expression levels of Mst1/2, phospho (p)-ALK, p-JNK, Nek7, and NLRP3 downstream molecules including activated caspase- 1 (p20) and mature interleukin (IL)-1β (p17), were upregulated in L. monocytogenes-infected macrophages. The ALK inhibitor significantly decreased the expression of p-JNK, Nek7, and NLRP3 downstream molecules in macrophages infected with L. monocytogenes. Furthermore, the Mst1/2 inhibitor markedly inhibited the L. monocytogenes-induced activation of ALK, subsequently downregulating the expression of p-JNK, Nek7, and NLRP3 downstream molecules. Therefore, our study demonstrated that Mst1/2-ALK mediated the activation of the NLRP3 inflammasome by promoting the interaction between Nek7 and NLRP3 via JNK during L. monocytogenes infection, which subsequently increased the maturation and release of proinflammatory cytokine to resist pathogen infection. Moreover, Listeriolysin O played a key role in the process. In addition, we also found that the L. monocytogenes-induced apoptosis of J774A.1 cells was reduced by the Mst1/2 or ALK inhibitor. The present study reported, for the first time, that the Mst1/2-ALK-JNK-NLRP3 signaling pathway plays a vital proinflammatory role during L. monocytogenes infection.

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  • IL-18 biology in severe asthma
    Sarita Thawanaphong, Aswathi Nair, Emily Volfson, Parameswaran Nair, Manali Mukherjee
    Frontiers in Medicine.2024;[Epub]     CrossRef
  • TRAF6-TAK1-IKKβ pathway mediates TLR2 agonists activating “one-step” NLRP3 inflammasome in human monocytes
    Mengdan Chen, Shi Yu, Yuhui Gao, Jiaxun Li, Xun Wang, Bin Wei, Guangxun Meng
    Cytokine.2023; 169: 156302.     CrossRef
  • ALK-JNK signaling promotes NLRP3 inflammasome activation and pyroptosis via NEK7 during Streptococcus pneumoniae infection
    Xia Wang, Yan Zhao, Dan Wang, Chang Liu, Zhi Qi, Huixin Tang, Yashan Liu, Shiqi Zhang, Yali Cui, Yingying Li, Ruiqing Liu, Yanna Shen
    Molecular Immunology.2023; 157: 78.     CrossRef
  • Inflammasome activation by Gram-positive bacteria: Mechanisms of activation and regulation
    A. Marijke Keestra-Gounder, Prescilla Emy Nagao
    Frontiers in Immunology.2023;[Epub]     CrossRef
  • Toxoplasma gondii profilin induces NLRP3 activation and IL-1β production/secretion in THP-1 cells
    Hossein Pazoki, Hamed Mirjalali, Maryam Niyyati, Seyed Javad Seyed Tabaei, Nariman Mosaffa, Shabnam Shahrokh, Hamid Asadzadeh Ahdaei, Andreas Kupz, Mohammad Reza Zali
    Microbial Pathogenesis.2023; 180: 106120.     CrossRef
  • The Critical Role of Potassium Efflux and Nek7 in Pasteurella multocida-Induced NLRP3 Inflammasome Activation
    Yu Wang, Zheng Zeng, Jinrong Ran, Lianci Peng, Xingping Wu, Chao Ye, Chunxia Dong, Yuanyi Peng, Rendong Fang
    Frontiers in Microbiology.2022;[Epub]     CrossRef
  • Coral and it's symbionts responses to the typical global marine pollutant BaP by 4D-Proteomics approach
    Yuebin Pei, Shuai Chen, Yuting Zhang, Volovych Olga, Yuanchao Li, Xiaoping Diao, Hailong Zhou
    Environmental Pollution.2022; 307: 119440.     CrossRef
  • NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection
    Ruiqing Liu, Yashan Liu, Chang Liu, Aijiao Gao, Lin Wang, Huixin Tang, Qiang Wu, Xia Wang, Derun Tian, Zhi Qi, Yanna Shen
    Frontiers in Immunology.2021;[Epub]     CrossRef
Comparative analysis of type 2 diabetes-associated gut microbiota between Han and Mongolian people
Shu-chun Li , Yao Xiao , Ri-tu Wu , Dan Xie , Huan-hu Zhao , Gang-yi Shen , En-qi Wu
J. Microbiol. 2021;59(7):693-701.   Published online May 15, 2021
DOI: https://doi.org/10.1007/s12275-021-0454-8
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AbstractAbstract
Due to the different rates of diabetes in different ethnic groups and the structural differences in intestinal microbiota, this study evaluated the changes in diabetes-related intestinal microbiota in two ethnic groups. Fifty-six stool samples were collected from subjects from the Han and Mongolian ethnic groups in China, including participants without diabetes (non-diabetic, ND) and with type 2 diabetes (T2D). The 16S rDNA gene V3 + V4 area was extracted from microbiota, amplified by PCR, and used to perform high-throughput sequencing and screen differential microbiota associated with ethnicity. The results showed that there were 44 T2D-related bacterial markers in the Han subjects, of which Flavonifractor, Alistipes, Prevotella, Oscillibacter, Clostridium XlVa, and Lachnospiracea_incertae_sedis were most closely related to diabetes. There were 20 T2D-related bacterial markers in the Mongolian subjects, of which Fastidiosipila and Barnesiella were most closely related to diabetes. The common markers of T2D bacteria in the two ethnic groups were Papillibacter and Bifidobacterium. There were 17 metabolic pathways with significant differences between the ND and T2D groups in the Han group, and 29 metabolic pathways in the Mongolian group. The glutamatergic metabolic pathway was the only common metabolic pathway in two ethnic groups. The composition and function of diabetes-related bacteria were significantly different among the different ethnic groups, which suggested that the influence of ethnic differences should be fully considered when studying the association between diabetes and bacteria. In addition, the common bacterial markers found in diabetic patients of different ethnic groups in this study can be used as potential targets to study the pathogenesis and treatment of diabetes.

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    Dan-Qian Chen, Hao-Jun Zhang, Wen Zhang, Kai Feng, Hui Liu, Hai-Ling Zhao, Ping Li
    Experimental Gerontology.2024; 188: 112393.     CrossRef
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    Yanchao Liu, Mingxiao Wang, Wuyuntana Li, Yumin Gao, Hailing Li, Ning Cao, Wenli Hao, Lingyan Zhao
    BMC Microbiology.2023;[Epub]     CrossRef
  • Bi-Directional Interactions between Glucose-Lowering Medications and Gut Microbiome in Patients with Type 2 Diabetes Mellitus: A Systematic Review
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Genetic changes in plaque-purified varicella vaccine strain Suduvax during in vitro propagation in cell culture
Hye Rim Hwang , Se Hwan Kang , Chan Hee Lee
J. Microbiol. 2021;59(7):702-707.   Published online June 1, 2021
DOI: https://doi.org/10.1007/s12275-021-1062-3
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AbstractAbstract
Infection by varicella-zoster virus (VZV) can be prevented by using live attenuated vaccines. VZV vaccine strains are known to evolve rapidly in vivo, however, their genetic and biological effects are not known. In this study, the plaque-purified vaccine strain Suduvax (PPS) was used to understand the genetic changes that occur during the process of propagation in in vitro cell culture. Full genome sequences of three different passages (p4, p30, and p60) of PPS were determined and compared for genetic changes. Mutations were found at 59 positions. The number of genetically polymorphic sites (GPS) and the average of minor allele frequency (MAF) at GPSs were not significantly altered after passaging in cell culture up to p60. The number of variant nucleotide positions (VNPs), wherein GPS was found in at least one passage of PPS, was 149. Overall, MAF changed by less than 5% at 52 VNPs, increased by more than 5% at 42 VNPs, and decreased by more than 5% at 55 VNPs in p60, compared with that seen in p4. More complicated patterns of changes in MAF were observed when genetic polymorphism at 149 VNPs was analyzed among the three passages. However, MAF decreased and mixed genotypes became unequivocally fixed to vaccine type in 23 vaccine-specific positions in higher passages of PPS. Plaque-purified Suduvax appeared to adapt to better replication during in vitro cell culture. Further studies with other vaccine strains and in vivo studies will help to understand the evolution of the VZV vaccine.

Journal of Microbiology : Journal of Microbiology
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