Journal of Microbiology

Volume 53(8); August 2015

Review

MINIREVIEW] Regulation of Escherichia coli RNase III activity: Boram Lim , Minji Sim , Howoon Lee , Seogang Hyun , Younghoon Lee , Yoonsoo Hahn , Eunkyoung Shin , Kangseok Lee; J. Microbiol. 2015;53(8):487-494. Published online July 31, 2015; DOI: https://doi.org/10.1007/s12275-015-5323-x

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Bacterial cells respond to changes in the environment by adjusting their physiological reactions. In cascades of cellular responses to stresses of various origins, rapid modulation of RNA function is known to be an effective biochemical adaptation. Among many factors affecting RNA function, RNase III, a member of the phylogenetically highly conserved endoribonuclease III family, plays a key role in posttranscriptional regulatory pathways in Escherichia coli. In this review, we provide an overview of the factors affecting RNase III activity in E. coli.

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Seungmok Han, Ji-Won Byun, Minho Lee
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Rosa Morra, Fenryco Pratama, Thomas Butterfield, Geizecler Tomazetto, Kate Young, Ruth Lopez, Neil Dixon
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Transcriptome and metabolome analyses of response of Synechocystis sp. PCC 6803 to methyl viologen
Xinyu Hu, Tianyuan Zhang, Kai Ji, Ke Luo, Li Wang, Wenli Chen
Applied Microbiology and Biotechnology.2021; 105(21-22): 8377. CrossRef
Endoribonuclease-mediated control of hns mRNA stability constitutes a key regulatory pathway for Salmonella Typhimurium pathogenicity island 1 expression
Minho Lee, Minkyung Ryu, Minju Joo, Young-Jin Seo, Jaejin Lee, Hong-Man Kim, Eunkyoung Shin, Ji-Hyun Yeom, Yong-Hak Kim, Jeehyeon Bae, Kangseok Lee, William Navarre
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Trans-acting regulators of ribonuclease activity
Jaejin Lee, Minho Lee, Kangseok Lee
Journal of Microbiology.2021; 59(4): 341. CrossRef
RNase III, Ribosome Biogenesis and Beyond
Maxence Lejars, Asaki Kobayashi, Eliane Hajnsdorf
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The rnc Gene Regulates the Microstructure of Exopolysaccharide in the Biofilm of Streptococcus mutans through the β-Monosaccharides
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Jing Zhao, Michael E Harris
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RNase G controls tpiA mRNA abundance in response to oxygen availability in Escherichia coli
Jaejin Lee, Dong-Ho Lee, Che Ok Jeon, Kangseok Lee
Journal of Microbiology.2019; 57(10): 910. CrossRef
The coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli
Minho Lee, Minju Joo, Minji Sim, Se-Hoon Sim, Hyun-Lee Kim, Jaejin Lee, Minkyung Ryu, Ji-Hyun Yeom, Yoonsoo Hahn, Nam-Chul Ha, Jang-Cheon Cho, Kangseok Lee
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Identification of endoribonuclease specific cleavage positions reveals novel targets of RNase III inStreptococcus pyogenes
Anaïs Le Rhun, Anne-Laure Lécrivain, Johan Reimegård, Estelle Proux-Wéra, Laura Broglia, Cristina Della Beffa, Emmanuelle Charpentier
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Research Support, Non-U.S. Gov't

Aliisedimentitalea scapharcae gen. nov., sp. nov., isolated from ark shell Scapharca broughtonii: Young-Ok Kim , Sooyeon Park , Bo-Hye Nam , Dong-Gyun Kim , Sung-Min Won , Ji-Min Park , Jung-Hoon Yoon; J. Microbiol. 2015;53(8):495-502. Published online July 31, 2015; DOI: https://doi.org/10.1007/s12275-015-5075-7

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Abstract

A Gram-negative, aerobic, non-spore-forming, motile and ovoid or rod-shaped bacterial strain, designated MA2-16T, was isolated from ark shell (Scapharca broughtonii) collected from the South Sea, South Korea. Strain MA2-16T was found to grow optimally at 30캜, at pH 7.0?.0 and in the presence of 2.0% (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain MA2-16T clustered with the type strain of Sedimentitalea nanhaiensis. The novel strain exhibited a 16S rRNA gene sequence similarity value of 97.1% to the type strain of S. nanhaiensis. In the neighbour- joining phylogenetic tree based on gyrB sequences, strain MA2-16T formed an evolutionary lineage independent of those of other taxa. Strain MA2-16T contained Q-10 as the predominant ubiquinone and C18:1 ?c and 11-methyl C18:1 ?c as the major fatty acids. The major polar lipids of strain MA2-16T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid and an unidentified lipid. The DNA G+C content of strain MA2- 16T was 57.7 mol% and its DNA-DNA relatedness values with the type strains of S. nanhaiensis and some phylogenetically related species of the genera Leisingera and Phaeobacter were 13?4%. On the basis of the data presented, strain MA2-16T is considered to represent a novel genus and novel species within the family Rhodobacteraceae, for which the name Aliisedimentitalea scapharcae gen. nov., sp. nov. is proposed. The type strain is MA2-16T (=KCTC 42119T =CECT 8598T).

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Jindonia aestuariivivens gen. nov., sp. nov., isolated from a tidal flat on the south-western sea in Republic of Korea
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Journal Article

Description of a novel pectin-degrading bacterial species Prevotella pectinovora sp. nov., based on its phenotypic and genomic traits: Brigita Nograsek , Tomaz Accetto , Lijana Fanedl , Gorazd Avgustin; J. Microbiol. 2015;53(8):503-510. Published online July 31, 2015; DOI: https://doi.org/10.1007/s12275-015-5142-0

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Abstract

Five strictly anaerobic Gram-negative bacterial strains, P4-65, P4-76T, P5-60, P5-119, and P5-125, presumably belonging to the genus Prevotella were isolated from pig fecal samples. Strains were tested for various phenotypic traits and nearcomplete genome sequences were obtained and analyzed. Phylogenetic analysis based on 16S rRNA gene sequences and multilocus sequence analysis based on five conserved genes confirmed that the strains belong to the genus Prevotella, revealing that they represent a novel and discrete lineage distinct from other known species of this genus. The size of the genome of the isolated strains is 3?.3 Mbp, and the DNA G+C content is 47.5?8.1 mol%. The isolates are strictly anaerobic, rod-shaped with rounded ends, non-motile and non-spore-forming. The main fermentation products are succinate and acetate, with minor concentrations of isovalerate, propionate and isobutyrate. Hydrogen is also produced. Major cellular fatty acids consist of anteiso-C15:0 and iso-C15:0, and a number of additional acids are present in lower concentrations. A substantial portion of genes involved in carbohydrate utilization is devoted to pectin degradation and utilization, while those supporting growth on xylan in ruminal Prevotella could not have been revealed. On the basis of the presented results, a novel species, Prevotella pectinovora sp. nov. is proposed. The type strain is P4-76T (=DSM 29996T =ZIM B1020T).

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Isolation of an algicide from a marine bacterium and its effects against the toxic dinoflagellate Alexandrium catenella and other harmful algal bloom species: Yun Sook Kim , Hong-Joo Son , Seong-Yun Jeong; J. Microbiol. 2015;53(8):511-517. Published online July 31, 2015; DOI: https://doi.org/10.1007/s12275-015-5303-1

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Abstract

The aim of this study was to isolate and identify bacteria demonstrating an algicidal effect against Alexandrium catenella and to determine the activity and range of any algicide discovered. The morphological and biochemical attributes of an algicidal bacterium, isolate YS-3, and analysis of its 16S rRNA gene sequence revealed it to be a member of the genus Brachybacterium. This organism, designated Brachybacterium sp. YS-3, showed the greatest effect against A. catenella cells of all bacteria isolated, and is assumed to produce secondary metabolites. When 10% solutions of culture filtrates from this strain were applied to A. catenella cultures, over 90% of cells were killed within 9 h. Bioassay-guided isolation of the algicide involved led to the purification and identification of an active compound. Based on physicochemical and spectroscopic data, including nuclear magnetic resonance and mass analyses, this compound was identified as 1-acetyl-β-carboline. This algicide showed significant activity against A. catenella and a wide range of harmful algal bloom (HAB)-forming species. Taken together, our results suggest that Brachybacterium sp. YS-3 and its algicide represent promising candidates for use in HAB control.

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Journal Article

Characterization of MocR, a GntR-like transcriptional regulator, in Bradyrhizobium japonicum: its impact on motility, biofilm formation, and soybean nodulation: May Nyan Taw , Hae-In Lee , Sang-Ho Lee , Woo-Suk Chang; J. Microbiol. 2015;53(8):518-525. Published online July 31, 2015; DOI: https://doi.org/10.1007/s12275-015-5313-z

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Abstract

Bradyrhizobium japonicum is a Gram-negative soil bacterium that can fix nitrogen into ammonia by developing a symbiotic relationship with the soybean plant. MocR proteins make up a subfamily of GntR superfamily, one of the most widely distributed and prolific groups of the helix-turn-helix transcription factors. In this study, we constructed a mutant strain for mocR (blr6977) to investigate its role in cellular processes and symbiosis in B. japonicum. Although growth rate and morphology of the mutant were indistinguishable from those of the wild type, the mutant showed significant differences in motility and attachment (i.e., biofilm formation) from the wild type. The mutant displayed a decrease in biofilm formation, but was more motile than the wild type. The inactivation of mocR did not affect the number of nodules on soybean roots, but caused delayed nodulation. Delayed nodulation intrigued us to study competitiveness of the mutant infecting soybeans. The mutant was less competitive than the wild type, indicating that delayed nodulation might be due to competitiveness. Gene expressions of other MocR subfamily members were also compared between the wild type and mutant strains. None of the mocR-like genes examined in this study were differentially expressed between both strains.

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The MocR family transcriptional regulator DnfR has multiple binding sites and regulates Dirammox gene transcription in Alcaligenes faecalis JQ135
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Lei Pan, Christopher L. Gardner, Reagan Beliakoff, Danilo da Silva, Ran Zuo, Fernando A. Pagliai, Kaylie A. Padgett‐Pagliai, Marcelo L. Merli, Erol Bahadiroglu, Claudio F. Gonzalez, Graciela L. Lorca
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Angela Tramonti, Caterina Nardella, Martino L. di Salvo, Stefano Pascarella, Roberto Contestabile
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Research Support, Non-U.S. Gov'ts

Role of the extracytoplasmic function sigma factor CarQ in oxidative response of Bradyrhizobium japonicum: Anchana Thaweethawakorn , Dylan Parks , Jae-Seong So , Woo-Suk Chang; J. Microbiol. 2015;53(8):526-534. Published online July 31, 2015; DOI: https://doi.org/10.1007/s12275-015-5308-9

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Abstract

As a nitrogen-fixing bacterium, Bradyrhizobium japonicum can establish a symbiotic relationship with the soybean plant (Glycine max). To be a successful symbiont, B. japonicum must deal with plant defense responses, such as an oxidative burst. Our previous functional genomics study showed that carQ (bll1028) encoding extracytoplasmic function (ECF) sigma factor was highly expressed (107.8-fold induction) under oxidative stress. Little is known about the underlying mechanisms of how CarQ responds to oxidative stress. In this study, a carQ knock-out mutant was constructed using site-specific mutagenesis to identify the role of carQ in the oxidative response of B. japonicum. The carQ mutant showed a longer generation time than the wild type and exhibited significantly decreased survival at 10 mM H2O2 for 10 min of exposure. Surprisingly, there was no significant difference in expression of oxidative stress-responsive genes such as katG and sod between the wild type and carQ mutant. The mutant also showed a significant increase in susceptibility to H2O2 compared to the wild type in the zone inhibition assay. Nodulation phenotypes of the carQ mutant were distinguishable compared to those of the wild type, including lower numbers of nodules, decreased nodule dry weight, decreased plant dry weight, and a lower nitrogen fixation capability. Moreover, desiccation of mutant cells also resulted in significantly lower percent of survival in both early (after 4 h) and late (after 24 h) desiccation periods. Taken together, this information will provide an insight into the role of the ECF sigma factor in B. japonicum to deal with a plant-derived oxidative burst.

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Claus Lang, Melanie J. Barnett, Robert F. Fisher, Lucinda S. Smith, Michelle E. Diodati, Sharon R. Long, Craig D. Ellermeier, Claude Bruand, Sarah Ades, Hans-Martin Fischer
mSphere.2018;[Epub] CrossRef

Functional properties of the major outer membrane protein in Stenotrophomonas maltophilia: Yih-Yuan Chen , Han-Chiang Wu , Juey-Wen Lin , Shu-Fen Weng; J. Microbiol. 2015;53(8):535-543. Published online July 31, 2015; DOI: https://doi.org/10.1007/s12275-015-5202-5

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Abstract

Stenotrophomonas maltophilia is an opportunistic pathogen that is closely associated with high morbidity and mortality in debilitated and immunocompromised individuals. Therefore, to investigate the pathogenesis mechanism is urgently required. However, there are very few studies to evaluate the functional properties of outer membrane protein, which may contribute to the pathogenesis in S. maltophilia. In this study, three abundant proteins in the outer membrane fraction of S. maltophilia were identified by liquid chromatography- tandem mass spectrometry as OmpW1, MopB, and a hypothetical protein. MopB, a member of the OmpA family, was firstly chosen for functional investigation in this study because many OmpA-family proteins are known to be involved in pathogenesis and offer potential as vaccines. Membrane fractionation analyses demonstrated that MopB was indeed the most abundant outer membrane protein (OMP) in S. maltophilia. For functional studies, the mopB mutant of S. maltophilia (SmMopB) was constructed by insertional mutation. MopB deficiency resulted in a change in the protein composition of OMPs and altered the architecture of the outer membrane. The SmMopB strain exhibited reduced cytotoxicity toward L929 fibroblasts and was more sensitive to numerous stresses, including human serum, sodium dodecyl sulfate, and hydrogen peroxide compared with wildtype S. maltophilia. These results suggest that MopB may be a good candidate for the design of vaccines or anti-MopB drugs for controlling serious nosocomial infections of multidrug- resistant S. maltophilia, especially in immunosuppressed patients.

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σ P -NagA-L1/L2 Regulatory Circuit Involved in ΔompA 299-356 -Mediated Increase in β-Lactam Susceptibility in Stenotrophomonas maltophilia
Li-Hua Li, Cheng-Mu Wu, Chia-Lun Chang, Hsin-Hui Huang, Chao-Jung Wu, Tsuey-Ching Yang, Silvia T. Cardona
Microbiology Spectrum.2022;[Epub] CrossRef
Advances in the Microbiology of Stenotrophomonas maltophilia
Joanna S. Brooke
Clinical Microbiology Reviews.2021;[Epub] CrossRef
Recombinant Ax21 protein is a promising subunit vaccine candidate against Stenotrophomonas maltophilia in a murine infection model
Amal Tarek Sarhan, Mohammed Bahey-El-Din, Taha Ibrahim Zaghloul
Vaccine.2021; 39(32): 4471. CrossRef
Intranasal immunization with recombinant outer membrane protein A induces protective immune response against Stenotrophomonas maltophilia infection
Yan Li, Xueping Tang, Zunquan Zhao, Hui Wang, Xin Wang, Xueyi Shang, Peng Liu, Zhihua Kou, Yongqiang Jiang, Yan Li, Daniela Flavia Hozbor
PLOS ONE.2019; 14(4): e0214596. CrossRef
The Major Outer Membrane Protein MopB Is Required for Twitching Movement and Affects Biofilm Formation and Virulence in Two Xylella fastidiosa strains
Hongyu Chen, Prem P. Kandel, Luisa F. Cruz, Paul A. Cobine, Leonardo De La Fuente
Molecular Plant-Microbe Interactions®.2017; 30(11): 896. CrossRef

Journal Articles

The role of programmed death ligand 1 pathway in persistent biomaterial-associated infections: Agnieszka Magrys , Jolanta Paluch-Oles , Agnieszka Bogut , Michal Kielbus , Dorota Plewik , Maria Koziol-Montewka; J. Microbiol. 2015;53(8):544-552. Published online July 31, 2015; DOI: https://doi.org/10.1007/s12275-015-5022-7

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Abstract

Staphylococcus epidermidis is commonly involved in biomaterial- associated infections. Bacterial small colony variants (SCV) seem to be well adapted to persist intracellularly in professional phagocytes evading the host immune response. We studied the expression of PD-L1/L2 on macrophages infected with clinical isolates of S. epidermidis SCV and their parent wild type (WT) strains. The cytokine pattern which is triggered by the examined strains was also analysed. In the study, we infected macrophages with S. epidermidis WT and SCV strains. Persistence and release from macrophages were monitored via lysostaphin protection assays. Moreover, the effect of IFN-γ pre-treatment on bacterial internalisation was investigated. Expression of PD-L1/L2 molecules was analysed with the use of FACS. Inflammatory reaction was measured by IL-10, TNF-α ELISAs, and transcriptional induction of TNF-α. Our study revealed that clinical SCV isolates were able to persist and survive in macrophages for at least 3 days with a low cytotoxic effect and a reduced proinflammatory response as compared to WT strains. Bacteria upregulated PD-L1/L2 expression on macrophages as compared to non-stimulated cells. The results demonstrated that the ability of S. epidermidis SCVs to induce elevated levels of anti-inflammatory cytokine, IL-10, and reduced transcriptional induction of TNF-α, together with expression of PD-L1 on macrophages and the ability to persist intracellularly without damaging the host cell could be the key factor contributing to chronicity of SCV infections.

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The mononuclear phagocyte system obscures the accurate diagnosis of infected joint replacements
Robert Manasherob, Shay I. Warren, Prerna Arora, Lyong Heo, Naomi L. Haddock, Ievgen Koliesnik, Diasuke Furukawa, Z. Ngalo Otieno-Ayayo, William J. Maloney, David W. Lowenberg, Stuart B. Goodman, Derek F. Amanatullah
Journal of Translational Medicine.2024;[Epub] CrossRef
Periprosthetic joint infection and immunity: Current understanding of host–microbe interplay
Nicolas S. Piuzzi, Alison K. Klika, Qiuhe Lu, Carlos A. Higuera‐Rueda, Thaddeus Stappenbeck, Anabelle Visperas
Journal of Orthopaedic Research.2024; 42(1): 7. CrossRef
The first genomic characterization of a stable, hemin-dependent small colony variant strain of Staphylococcus epidermidis isolated from a prosthetic-joint infection
Agnieszka Bogut, Piotr Koper, Małgorzata Marczak, Paulina Całka
Frontiers in Microbiology.2023;[Epub] CrossRef
Immune checkpoint upregulation in periprosthetic joint infection
Shay I. Warren, Gregory W. Charville, Robert Manasherob, Derek F. Amanatullah
Journal of Orthopaedic Research.2022; 40(11): 2663. CrossRef
The road to success of coagulase-negative staphylococci: clinical significance of small colony variants and their pathogenic role in persistent infections
Agnieszka Bogut, Agnieszka Magryś
European Journal of Clinical Microbiology & Infectious Diseases.2021; 40(11): 2249. CrossRef
Chronic Implant-Related Bone Infections—Can Immune Modulation be a Therapeutic Strategy?
Elisabeth Seebach, Katharina F. Kubatzky
Frontiers in Immunology.2019;[Epub] CrossRef
Expression of programmed death ligand‐1 on bladder tissues is detected in a clinically and histologically well‐defined interstitial cystitis cohort
Yuke Chen, Wei Yu, Yang Yang, Yunxiang Xiao, Yun Cui, Jihong Duan, Qun He, Jie Jin, Shiliang Wu
Neurourology and Urodynamics.2018; 37(4): 1396. CrossRef
The role of the PI3K/mTOR signaling pathway in Staphylococcus epidermidis small colony variants intracellular survival
Agnieszka Magryś, Agnieszka Bogut, Michał Kiełbus, Alina Olender
Immunological Investigations.2018; 47(3): 251. CrossRef
Intraphagolysosomal conditions predispose to Staphylococcus epidermidis small colony variants persistence in macrophages
Agnieszka Magryś, Kamil Deryło, Agnieszka Bogut, Alina Olender, Marek Tchórzewski, Holger Rohde
PLOS ONE.2018; 13(11): e0207312. CrossRef
Pathogenic Mechanisms and Host Interactions in Staphylococcus epidermidis Device-Related Infection
Marina Sabaté Brescó, Llinos G. Harris, Keith Thompson, Barbara Stanic, Mario Morgenstern, Liam O'Mahony, R. Geoff Richards, T. Fintan Moriarty
Frontiers in Microbiology.2017;[Epub] CrossRef

Antagonistic effect of peptidoglycan of Streptococcus sanguinis on lipopolysaccharide of major periodontal pathogens: Sung-Hoon Lee; J. Microbiol. 2015;53(8):553-560. Published online July 31, 2015; DOI: https://doi.org/10.1007/s12275-015-5319-6

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Abstract

Streptococcus sanguinis is often found in subgingival biofilm including periodontopathogens, and is correlated with a delay in colonization by periodontopathogens. However, the effect of S. sanguinis on inflammation induced by periodontopathogens is poorly understood. Thus, this study investigated the effect of S. sanguinis peptidoglycan (PGN) on induction of TNF-α, IL-6, and IL-8 expression by lipopolysaccharide (LPS) of periodontal pathogens. LPS was extracted from Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythia, and PGN was isolated from S. sanguinis. THP-1 cells, a monocytic cell-line, were cotreated with LPS of the periodontal pathogens and S. sanguinis PGN, and then the expression of inflammatory cytokines was analyzed by real-time RT-PCR. To analyze the underlying mechanism, the binding assay of the LPS to CD14 or LPS-binding protein (LBP) was performed in the presence or absence of the PGN after coating recombinant human CD14 and LBP on EIA plate. The PGN inhibited the binding of LPS to CD14 and LBP in a dose-dependent manner. Also, THP-1 cells were co-treated with the LPS in the presence of N-acetylmuramic acid and N-acetylglucosamine, as components of PGN, and the competition binding assay to CD14 and LBP was performed. N-acetylmuramic acid inhibited the induction of inflammatory cytokine expression by LPS and the binding of LPS to CD14 or LBP whereas Nacetylglucosamine did not show such effect. Collectively, the
results
suggest that S. sanguinis PGN inhibited the cytokine expression induced by the LPS of periodontopathogens due to the inhibition of LPS binding to LBP and CD14. N-acetylmuramic acid of PGN may play a role in inhibition of the LPS binding of periodontopathogens to CD14 and LBP.

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Inflammasome regulation by the cell surface ecto-5′-nucleotidase of the oral commensal, Streptococcus oralis
Natsuno Nakamura, Hirobumi Morisaki, Momoe Itsumi, Nobuo Okahashi, Haruka Fukamachi, Ayako Sato, Miki Kadena, Mariko Kikuchi, Shohei Matsui, Takahiro Funatsu, Hirotaka Kuwata
Biochemical and Biophysical Research Communications.2025; 744: 151206. CrossRef
New putative periodontopathogens and periodontal health‐associated species: A systematic review and meta‐analysis
Angéline Antezack, Damien Etchecopar‐Etchart, Bernard La Scola, Virginie Monnet‐Corti
Journal of Periodontal Research.2023; 58(5): 893. CrossRef
Correlation and mechanism between cardiac magnetic resonance imaging and oral streptococcus count in patients with primary microvascular angina pectoris
Qi Huang, Shi Sheng Wang, Rong Hua Luo
Medicine.2022; 101(12): e29060. CrossRef
Oral ecological environment modifications by hard-cheese: from pH to microbiome: a prospective cohort study based on 16S rRNA metabarcoding approach
Erna Cecilia Lorenzini, Barbara Lazzari, Gianluca Martino Tartaglia, Giampietro Farronato, Valentina Lanteri, Sara Botti, Filippo Biscarini, Paolo Cozzi, Alessandra Stella
Journal of Translational Medicine.2022;[Epub] CrossRef
Biofilm growth and IL-8 & TNF-α-inducing properties of Candida albicans in the presence of oral gram-positive and gram-negative bacteria
Radhika G. Bhardwaj, Arjuna Ellepolla, Hana Drobiova, Maribasappa Karched
BMC Microbiology.2020;[Epub] CrossRef
Genetics ofsanguinis-Group Streptococci in Health and Disease
Angela Nobbs, Jens Kreth, Vincent A. Fischetti, Richard P. Novick, Joseph J. Ferretti, Daniel A. Portnoy, Miriam Braunstein, Julian I. Rood
Microbiology Spectrum.2019;[Epub] CrossRef
Influence of a light‐activated glaze on the adhesion of Streptococcus sanguinis to the surface of polymers used in fabrication of interim prostheses
Daniela Micheline dos Santos, Betina Chiarelo Commar, Emily Vivianne Freitas da Silva, Valentim Adelino Ricardo Barão, Adaias Oliveira Matos, Marcelo Coelho Goiato
Journal of Investigative and Clinical Dentistry.2019;[Epub] CrossRef
Novel nanotechnology and near-infrared photodynamic therapy to kill periodontitis-related biofilm pathogens and protect the periodontium
Manlin Qi, Xue Li, Xiaolin Sun, Chunyan Li, Franklin R. Tay, Michael D. Weir, Biao Dong, Yanmin Zhou, Lin Wang, Hockin H.K. Xu
Dental Materials.2019; 35(11): 1665. CrossRef
A wear-resistant TiO2 nanoceramic coating on titanium implants for visible-light photocatalytic removal of organic residues
Hao Wu, Li Xie, Min He, Ruitao Zhang, Yuan Tian, Suru Liu, Tao Gong, Fangjun Huo, Ting Yang, Qingyuan Zhang, Shujuan Guo, Weidong Tian
Acta Biomaterialia.2019; 97: 597. CrossRef
Activity of the Chimeric Lysin ClyR against Common Gram-Positive Oral Microbes and Its Anticaries Efficacy in Rat Models
Jingjing Xu, Hang Yang, Yongli Bi, Wuyou Li, Hongping Wei, Yuhong Li
Viruses.2018; 10(7): 380. CrossRef
Bacterial Adhesion on Lithium Disilicate Ceramic Surface Exposed to Different Hydrofluoric Solutions
Daniela Micheline dos Santos, Emily Vivianne Freitas da Silva, Adaias Oliveira Matos, Beatriz Cristiane Zuin Monteiro, Rodrigo Antonio de Medeiros, Sandro Basso Bitencourt, Valentim Adelino Ricardo Barão, Elidiane Cipriano Rangel, Marcelo Coelho Goiato
Ceramics.2018; 1(1): 145. CrossRef
Inhibitory effect of Lactococcus lactis on the bioactivity of periodontopathogens
Hyun-Seung Shin, Dong-Heon Baek, Sung-Hoon Lee
The Journal of General and Applied Microbiology.2018; 64(2): 55. CrossRef
The road less traveled – defining molecular commensalism with Streptococcus sanguinis
J. Kreth, R.A. Giacaman, R. Raghavan, J. Merritt
Molecular Oral Microbiology.2017; 32(3): 181. CrossRef
Buckyballs conjugated with nucleic acid sequences identifies microorganisms in live cell assays
Qingsu Cheng, Bahram Parvin
Journal of Nanobiotechnology.2017;[Epub] CrossRef
Antimicrobial activity and regulation of CXCL9 and CXCL10 in oral keratinocytes
Alison Marshall, Antonio Celentano, Nicola Cirillo, Michele D. Mignogna, Michael McCullough, Stephen Porter
European Journal of Oral Sciences.2016; 124(5): 433. CrossRef

Research Support, Non-U.S. Gov'ts

Requirement of the N-terminal residues of human cytomegalovirus UL112-113 proteins for viral growth and oriLyt-dependent DNA replication: Young-Eui Kim , Mi Young Park , Kyeong Jin Kang , Tae Hee Han , Chan Hee Lee , Jin-Hyun Ahn; J. Microbiol. 2015;53(8):561-569. Published online July 31, 2015; DOI: https://doi.org/10.1007/s12275-015-5301-3

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Abstract

The UL112-113 region of the human cytomegalovirus (HCMV) genome encodes four phosphoproteins of 34, 43, 50, and 84 kDa that promote viral DNA replication. Co-transfection assays have demonstrated that self-interaction of these proteins via the shared N-termini is necessary for their intranuclear distribution as foci and for the efficient relocation of a viral DNA polymerase processivity factor (UL44) to the viral replication sites. However, the requirement of UL112- 113 N-terminal residues for viral growth and DNA replication has not been fully elucidated. Here, we investigated the effect of deletion of the N-terminal regions of UL112- 113 proteins on viral growth and oriLyt-dependent DNA replication. A deletion of the entire UL112 region or the region encoding the 25 N-terminal amino-acid residues from the HCMV (Towne strain) bacmid impaired viral growth in bacmid-transfected human fibroblast cells, indicating their requirement for viral growth. In co-immunoprecipitation assays using the genomic gene expressing the four UL112- 113 proteins together, the 25 N-terminal amino-acid residues were found to be necessary for stable expression of UL112- 113 proteins and their self-interaction. These residues were also required for efficient binding to and relocation of UL44, but not for interaction with IE2, an origin-binding transcription factor. In co-transfection/replication assays, replication of the oriLyt-containing plasmid was promoted by expression of intact UL112-113 proteins, but not by the expression of 25-amino-acid residue-deleted proteins. Our
results
demonstrate that the 25 N-terminal amino-acid residues of UL112-113 proteins that mediate self-interaction contribute to viral growth by promoting their binding to UL44 and the initiation of oriLyt-dependent DNA replication.

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Insights into the Transcriptome of Human Cytomegalovirus: A Comprehensive Review
Janine Zeng, Di Cao, Shaomin Yang, Dabbu Kumar Jaijyan, Xiaolian Liu, Songbin Wu, Ruth Cruz-Cosme, Qiyi Tang, Hua Zhu
Viruses.2023; 15(8): 1703. CrossRef
The human cytomegalovirus decathlon: Ten critical replication events provide opportunities for restriction
Declan L. Turner, Rommel A. Mathias
Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef
Degradation of SAMHD1 Restriction Factor Through Cullin-Ring E3 Ligase Complexes During Human Cytomegalovirus Infection
Seokhwan Hyeon, Myoung Kyu Lee, Young-Eui Kim, Gwang Myeong Lee, Jin-Hyun Ahn
Frontiers in Cellular and Infection Microbiology.2020;[Epub] CrossRef
Primary lymphocyte infection models for KSHV and its putative tumorigenesis mechanisms in B cell lymphomas
Sangmin Kang, Jinjong Myoung
Journal of Microbiology.2017; 55(5): 319. CrossRef
Differential Requirement of Human Cytomegalovirus UL112-113 Protein Isoforms for Viral Replication
Tim Schommartz, Jiajia Tang, Rebekka Brost, Wolfram Brune, Klaus Frueh
Journal of Virology.2017;[Epub] CrossRef

Molecular characterization of mammalian-adapted Korean-type avian H9N2 virus and evaluation of its virulence in mice: Kuk Jin Park , Min-Suk Song , Eun-Ha Kim , Hyeok-il Kwon , Yun Hee Baek , Eun-hye Choi , Su-Jin Park , Se Mi Kim , Young-il Kim , Won-Suk Choi , Dae-Won Yoo , Chul-Joong Kim , Young Ki Choi; J. Microbiol. 2015;53(8):570-577. Published online July 31, 2015; DOI: https://doi.org/10.1007/s12275-015-5329-4

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Abstract

Avian influenza A virus (AIV) is commonly isolated from domestic poultry and wild migratory birds, and the H9N2 subtype is the most prevalent and the major cause of severe disease in poultry in Korea. In addition to the veterinary concerns regarding the H9N2 subtype, it is also considered to be the next potential human pandemic strain due to its rapid evolution and interspecies transmission. In this study, we utilize serial lung-to-lung passage of a low pathogenic avian influenza virus (LPAI) H9N2 (A/Ck/Korea/163/04, WT163) (Y439-lineage) in mice to increase pathogenicity and investigate the potential virulence marker. Mouse-adapted H9N2 virus obtained high virulence (100% mortality) in mice after 98 serial passages. Sequence results show that the mouse adaptation (ma163) possesses several mutations within seven gene segments (PB2, PA, HA, NP, NA, M, and NS) relative to the wild-type strain. The HA gene showed the most mutations (at least 11) with one resulting in the loss of an N-glycosylation site (at amino acid 166). Moreover, reverse genetic studies established that an E627K substitution in PB2 and the loss of the N-glycosylation site in the HA protein (aa166) are critical virulence markers in the mouse-adapted H9N2 virus. Thus, these results add to the increasing body of mutational analysis data defining the function of the viral polymerase and HA genes and their roles in mammalian host adaptation. To our knowledge, this is first report of the generation of a mammalian-adapted Korea H9N2 virus (Y493-lineages). Therefore, this study offers valuable insights into the molecular evolution of the LPAI Korean H9N2 in a new host and adds to the current knowledge of the molecular markers associated with increased virulence.

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Genetic Characterization of Kazakhstan Isolates: Avian Influenza H9N2 Viruses Demonstrate Their Potential to Infect Mammals
Barshagul Baikara, Kobey Karamendin, Yermukhammet Kassymbekov, Klara Daulbayeva, Temirlan Sabyrzhan, Sardor Nuralibekov, Yelizaveta Khan, Nurlan Sandybayev, Sasan Fereidouni, Aidyn Kydyrmanov
Viruses.2025; 17(5): 685. CrossRef
An Influenza A virus can evolve to use human ANP32E through altering polymerase dimerization
Carol M. Sheppard, Daniel H. Goldhill, Olivia C. Swann, Ecco Staller, Rebecca Penn, Olivia K. Platt, Ksenia Sukhova, Laury Baillon, Rebecca Frise, Thomas P. Peacock, Ervin Fodor, Wendy S. Barclay
Nature Communications.2023;[Epub] CrossRef
Current situation and control strategies of H9N2 avian influenza in South Korea
Mingeun Sagong, Kwang-Nyeong Lee, Eun-Kyoung Lee, Hyunmi Kang, Young Ki Choi, Youn-Jeong Lee
Journal of Veterinary Science.2023;[Epub] CrossRef
Antigenic Evolution Characteristics and Immunological Evaluation of H9N2 Avian Influenza Viruses from 1994–2019 in China
Qingzheng Liu, Lingcai Zhao, Yanna Guo, Yongzhen Zhao, Yingfei Li, Na Chen, Yuanlu Lu, Mengqi Yu, Lulu Deng, Jihui Ping
Viruses.2022; 14(4): 726. CrossRef
Molecular epidemiology and pathogenicity of H5N1 and H9N2 avian influenza viruses in clinically affected chickens on farms in Bangladesh
Ripatun Nahar Ripa, Joshua E. Sealy, Jayna Raghwani, Tridip Das, Himel Barua, Md. Masuduzzaman, A. K. M. Saifuddin, Md. Reajul Huq, Mohammad Inkeyas Uddin, Munir Iqbal, Ian Brown, Nicola S. Lewis, Dirk Pfeiffer, Guillaume Fournie, Paritosh Kumar Biswas
Emerging Microbes & Infections.2021; 10(1): 2223. CrossRef
Mouse adaptation of the H9N2 avian influenza virus causes the downregulation of genes related to innate immune responses and ubiquitin-mediated proteolysis in mice
Jing Guo, Xinxin Gao, Baotao Liu, Yubao Li, Wenqiang Liu, Jianbiao Lu, Cheng Liu, Rui Xue, Xuyong Li
Medical Microbiology and Immunology.2020; 209(2): 151. CrossRef
H9 Influenza Viruses: An Emerging Challenge
Silvia Carnaccini, Daniel R. Perez
Cold Spring Harbor Perspectives in Medicine.2020; 10(6): a038588. CrossRef
Adaptive amino acid substitutions enable transmission of an H9N2 avian influenza virus in guinea pigs
Liu Lina, Chen Saijuan, Wang Chengyu, Lu Yuefeng, Dong Shishan, Chen Ligong, Guo Kangkang, Guo Zhendong, Li Jiakai, Zhang Jianhui, Luo Qingping, Zhang Wenting, Shang Yu, Wang Honglin, Zhang Tengfei, Wen Guoyuan, Zhu Jiping, Zhang Chunmao, Jin Meilin, Gao
Scientific Reports.2019;[Epub] CrossRef
A PB1-K577E Mutation in H9N2 Influenza Virus Increases Polymerase Activity and Pathogenicity in Mice
Haruhiko Kamiki, Hiromichi Matsugo, Tomoya Kobayashi, Hiroho Ishida, Akiko Takenaka-Uema, Shin Murakami, Taisuke Horimoto
Viruses.2018; 10(11): 653. CrossRef
Genetics and biological property analysis of Korea lineage of influenza A H9N2 viruses
Min Kang, Hyung-Kwan Jang
Veterinary Microbiology.2017; 204: 96. CrossRef
The significance of avian influenza virus mouse-adaptation and its application in characterizing the efficacy of new vaccines and therapeutic agents
Won-Suk Choi, Khristine Kaith S. Lloren, Yun Hee Baek, Min-Suk Song
Clinical and Experimental Vaccine Research.2017; 6(2): 83. CrossRef
Rapid acquisition of polymorphic virulence markers during adaptation of highly pathogenic avian influenza H5N8 virus in the mouse
Won-Suk Choi, Yun Hee Baek, Jin Jung Kwon, Ju Hwan Jeong, Su-Jin Park, Young-il Kim, Sun-Woo Yoon, Jungwon Hwang, Myung Hee Kim, Chul-Joong Kim, Richard J. Webby, Young Ki Choi, Min-Suk Song
Scientific Reports.2017;[Epub] CrossRef
Vaccine Efficacy of Inactivated, Chimeric Hemagglutinin H9/H5N2 Avian Influenza Virus and Its Suitability for the Marker Vaccine Strategy
Se Mi Kim, Young-Il Kim, Su-Jin Park, Eun-Ha Kim, Hyeok-il Kwon, Young-Jae Si, In-Won Lee, Min-Suk Song, Young Ki Choi, Jae U. Jung
Journal of Virology.2017;[Epub] CrossRef
Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014
Duong Mai Thuy, Thomas P. Peacock, Vu Thi Ngoc Bich, Thomas Fabrizio, Dang Nguyen Hoang, Nguyen Dang Tho, Nguyen Thi Diep, Minh Nguyen, Le Nguyen Minh Hoa, Hau Thi Thu Trang, Marc Choisy, Ken Inui, Scott Newman, Nguyen vu Trung, Rogier van Doorn, Thanh Lo
Infection, Genetics and Evolution.2016; 44: 530. CrossRef
PB2 subunit of avian influenza virus subtype H9N2: a pandemic risk factor
Hanna Sediri, Swantje Thiele, Folker Schwalm, Gülsah Gabriel, Hans-Dieter Klenk
Journal of General Virology.2016; 97(1): 39. CrossRef

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