Journal Article
- Hepatitis B virus (HBV) codon adapts well to the gene expression profile of liver cancer: an evolutionary explanation for HBV’s oncogenic role
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Chunpeng Yu , Jian Li , Qun Li , Shuai Chang , Yufeng Cao , Hui Jiang , Lingling Xie , Gang Fan , Song Wang
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J. Microbiol. 2022;60(11):1106-1112. Published online October 17, 2022
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DOI: https://doi.org/10.1007/s12275-022-2371-x
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Abstract
- Due to the evolutionary arms race between hosts and viruses,
viruses must adapt to host translation systems to rapidly synthesize
viral proteins. Highly expressed genes in hosts have a
codon bias related to tRNA abundance, the primary RNA translation
rate determinant. We calculated the relative synonymous
codon usage (RSCU) of three hepatitis viruses (HAV,
HBV, and HCV), SARS-CoV-2, 30 human tissues, and hepatocellular
carcinoma (HCC). After comparing RSCU between
viruses and human tissues, we calculated the codon adaptation
index (CAI) of viral and human genes. HBV and HCV
showed the highest correlations with HCC and the normal
liver, while SARS-CoV-2 had the strongest association with
lungs. In addition, based on HCC RSCU, the CAI of HBV and
HCV genes was the highest. HBV and HCV preferentially adapt
to the tRNA pool in HCC, facilitating viral RNA translation.
After an initial trigger, rapid HBV/HCV translation and replication
may change normal liver cells into HCC cells. Our
findings reveal a novel perspective on virus-mediated oncogenesis.
Research Support, Non-U.S. Gov't
- Factors Influencing Preferential Utilization of RNA Polymerase Containing Sigma-38 in Stationary-Phase Gene Expression in Escherichia coli
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Eun Young Kim , Min-Sang Shin , Joon Haeng Rhee , Hyon E. Choy
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J. Microbiol. 2004;42(2):103-110.
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DOI: https://doi.org/2037 [pii]
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Abstract
- In order to understand the molecular basis of selective expression of stationary-phase genes by RNA polymerase containing [sigma]^38 (E[sigma]^38) in Escherichia coli, we examined transcription from the stationaryphase promoters, katEP, bolAP, hdeABP, csgBAP, and mcbP, in vivo and in vitro. Although these promoters are preferentially recognized in vivo by E[sigma]^38, they are transcribed in vitro by both E[sigma]^38 and E[sigma]^70 containing the major exponential [sigma], [sigma]^70. In the presence of high concentrations of glutamate salts, however, only E[sigma]^38 was able to efficiently transcribe from these promoters, which supports the concept that the promoter selectivity of [sigma]^38 -containing RNA polymerase is observed only under specific reaction conditions. The examination of 6S RNA, which is encoded by the ssr1 gene in vivo, showed that it reduced E[sigma]^70 activity during the stationary phase, but this reduction of activity did not result in the elevation of E[sigma]^38 activity. Thus, the preferential expression of stationary-phase genes by E[sigma]^38 is unlikely the consequence of selective inhibition of E[sigma]^70 by 6S RNA.