Journal of Microbiology

Research Support, Non-U.S. Gov'ts

NOTE] Antifungal Activity of Extracellular Hydrolases Produced by Autolysing Aspergillus nidulans Cultures: Melinda Szilágyi , Fruzsina Anton , Katalin Forgács , Jae-Hyuk Yu , István Pócsi , Tamás Emri; J. Microbiol. 2012;50(5):849-854. Published online November 4, 2012; DOI: https://doi.org/10.1007/s12275-012-2001-0

42 View
0 Download
8 Crossref

Carbon-starving Aspergillus nidulans cultures produce high activities of versatile hydrolytic enzymes and, among these, ChiB endochitinase and EngA β-1,3-endoglucanase showed significant antifungal activity against various fungal species. Double deletion of engA and chiB diminished the antifungal activity of the fermentation broths and increased conidiogenesis and long-term viability of A. nidulans, but decreased the growth rate on culture media containing weak carbon sources. Production of ChiB and EngA can influence fungal communities either directly due to their antifungal properties or indirectly through their effects on vegetative growth. Our data suggest saprophytic fungi as promising future candidates to develop novel biocontrol technologies.

Citations

Citations to this article as recorded by

Application and antagonistic mechanisms of atoxigenic Aspergillus strains for the management of fungal plant diseases
Suyan Wang, Yanxia Wang, Xinchi Shi, Daniela D. Herrera-Balandrano, Xin Chen, Fengquan Liu, Pedro Laborda, Irina S. Druzhinina
Applied and Environmental Microbiology.2024;[Epub] CrossRef
Identification and evaluation of Aspergillus tubingensis as a potential biocontrol agent against grey mould on tomato
Juan Zhao, Weicheng Liu, Dewen Liu, Caige Lu, Dianpeng Zhang, Huiling Wu, Dan Dong, Lingling Meng
Journal of General Plant Pathology.2018; 84(2): 148. CrossRef
Autolytic enzymes are responsible for increased melanization of carbon stressed Aspergillus nidulans cultures
Melinda Szilágyi, Fruzsina Anton, István Pócsi, Tamás Emri
Journal of Basic Microbiology.2018; 58(5): 440. CrossRef
Tricking Arthrinium malaysianum into Producing Industrially Important Enzymes Under 2-Deoxy D-Glucose Treatment
Soumya Mukherjee, Mathu Malar Chandrababunaidu, Arijit Panda, Suman Khowala, Sucheta Tripathy
Frontiers in Microbiology.2016;[Epub] CrossRef
γ-Glutamyl transpeptidase (GgtA) of Aspergillus nidulans is not necessary for bulk degradation of glutathione
Zsolt Spitzmüller, Nak-Jung Kwon, Melinda Szilágyi, Judit Keserű, Viktória Tóth, Jae-Hyuk Yu, István Pócsi, Tamás Emri
Archives of Microbiology.2015; 197(2): 285. CrossRef
Investigating Aspergillus nidulans secretome during colonisation of cork cell walls
Isabel Martins, Helga Garcia, Adélia Varela, Oscar Núñez, Sébastien Planchon, Maria Teresa Galceran, Jenny Renaut, Luís P.N. Rebelo, Cristina Silva Pereira
Journal of Proteomics.2014; 98: 175. CrossRef
Transcriptome changes initiated by carbon starvation in Aspergillus nidulans
Melinda Szilágyi, Márton Miskei, Zsolt Karányi, Béla Lenkey, István Pócsi, Tamás Emri
Microbiology.2013; 159(Pt_1): 176. CrossRef
Interactions between naturally occurring antifungal agents
Viktória Tóth, Melinda Szilágyi, Fruzsina Anton, Éva Leiter, I. Pócsi, T. Emri
Acta Biologica Hungarica.2013; 64(4): 510. CrossRef

Predicting the Chemical Composition and Structure of Aspergillus nidulans Hyphal Wall Surface by Atomic Force Microscopy: Hyun-uk Lee , Jong Bae Park , Haeseong Lee , Keon-Sang Chae , Dong-Min Han , Kwang-Yeop Jahng; J. Microbiol. 2010;48(2):243-248. Published online May 1, 2010; DOI: https://doi.org/10.1007/s12275-010-8094-4

41 View
0 Download
7 Scopus

Abstract: In fungi, cell wall plays an important role in growth and development. Major macromolecular constituents of the aspergilli cell wall are glucan, chitin, and protein. We examined the chemical composition and structure of the Aspergillus nidulans hyphal wall surface by an atomic force microscope (AFM). To determine the composition of the cell wall surface, the adhesion forces of commercially available β-glucan, chitin, and various proteins were compared to those of corresponding fractions prepared from the hyphal wall. In both setups, the adhesion forces of β-glucan, chitin, and protein were 25-50, 1000-3000, and 125-300 nN, respectively. Adhesion force analysis demonstrated that the cell surface of the apical tip region might contain primarily chitin and β-glucan and relatively a little protein. This analysis also showed the chemical composition of the hyphal surface of the mid-region would be different from that of the apical region. Morphological images obtained by the tapping mode of AFM revealed that the hyphal tip surface has moderate roughness.

Differential Expression of citA Gene Encoding the Mitochondrial Citrate Synthase of Aspergillus nidulans in Response to Developmental Status and Carbon Sources: In Sook Min , Ji Young Bang , Soon Won Seo , Cheong Ho Lee , Pil Jae Maeng; J. Microbiol. 2010;48(2):188-198. Published online May 1, 2010; DOI: https://doi.org/10.1007/s12275-010-0096-8

53 View
0 Download
8 Crossref

Abstract

As an extension of our previous studies on the mitochondrial citrate synthase of Aspergillus nidulans and cloning of its coding gene (citA), we analyzed differential expression of citA in response to the progress of development and change of carbon source. The cDNA consisted of 1,700 nucleotides and was predicted to encode a 474-amino acid protein. By comparing the cDNA sequence with the corresponding genomic sequence, we confirmed that citA gene contains 7 introns and that its transcription starts at position -26 (26-nucleotide upstream from the initiation codon). Four putative CreA binding motifs and three putative stress-response elements (STREs) were found within the 1.45-kb citA promoter region. The mode of citA expression was examined by both Northern blot and confocal microscopy using green fluorescent protein (sGFP) as a vital reporter. During vegetative growth and asexual development, the expression of citA was ubiqiutous throughout the whole fungal body including mycelia and conidiophores. During sexual development, the expression of citA was quite strong in cleistothecial shells, but significantly weak in the content of cleistothecia including ascospores. Acetate showed a strong inductive effect on citA expression, which is subjected to carbon catabolite repression (CCR) caused by glucose. The recombinant fusion protein CitA40::sGFP (sGFP containing the 40-amino acid N-terminal segment of CitA) was localized into mitochondria, which supports that a mitochondrial targeting signal is included within the 40-amino acid N-terminal segment of CitA.

Citations

Citations to this article as recorded by

Efficient carbon flux allocation towards D-pantothenic acid production via growth-decoupled strategy in Escherichia coli
Yihong Wang, Junping Zhou, Zheng Zhang, Lianggang Huang, Bo Zhang, Zhiqiang Liu, Yuguo Zheng
Bioresource Technology.2024; 411: 131325. CrossRef
Shining a light on the impact of antifungals on Aspergillus fumigatus subcellular dynamics through fluorescence imaging
I. S. R. Storer, L. E. Sastré-Velásquez, T. Easter, B. Mertens, A. Dallemulle, M. Bottery, R. Tank, M. Offterdinger, M. J. Bromley, N. van Rhijn, F. Gsaller, Andreas H. Groll
Antimicrobial Agents and Chemotherapy.2024;[Epub] CrossRef
Multiplex Genetic Engineering Exploiting Pyrimidine Salvage Pathway-Based Endogenous Counterselectable Markers
Lukas Birštonas, Alex Dallemulle, Manuel S. López-Berges, Ilse D. Jacobsen, Martin Offterdinger, Beate Abt, Maria Straßburger, Ingo Bauer, Oliver Schmidt, Bettina Sarg, Herbert Lindner, Hubertus Haas, Fabio Gsaller, Gustavo H. Goldman
mBio.2020;[Epub] CrossRef
A novel citrate synthase isoform contributes infection and stress resistance of the stripe rust fungus
Dan Li, Lijing Pang, Pu Yuan, Peijing Zheng, Baoyu Huai, Mohan Yao, Zhensheng Kang, Jie Liu
Environmental Microbiology.2018; 20(11): 4037. CrossRef
In silico characterization of the citrate synthase family in Mycobacterium tuberculosis / Mycobacterium tuberculosis’te sitrat sentaz ailesinin in silico karakterizasyonu
Sezer Okay
Turkish Journal of Biochemistry.2016; 41(2): 118. CrossRef
Three genes encoding citrate synthases in Saccharopolyspora erythraea are regulated by the global nutrient‐sensing regulators GlnR, DasR, and CRP
Cheng‐Heng Liao, Li‐li Yao, Bang‐Ce Ye
Molecular Microbiology.2014; 94(5): 1065. CrossRef
Gene Identification and Functional Analysis of Methylcitrate Synthase in Citric Acid-ProducingAspergillus nigerWU-2223L
Keiichi KOBAYASHI, Takasumi HATTORI, Yuki HONDA, Kohtaro KIRIMURA
Bioscience, Biotechnology, and Biochemistry.2013; 77(7): 1492. CrossRef
Utility of Aspergillus niger citrate synthase promoter for heterologous expression
Kashyap Dave, Narayan S. Punekar
Journal of Biotechnology.2011; 155(2): 173. CrossRef

Quantitative analysis of gene expression pattern in aspergillus nidulans mycelia by sequencing of 3'-directed cDNA clones: Park, Yoon Dong , Lee, Dong Whan , Lee, Seog Jae , Kim, Jong Hwa , Chae, Keon Sang; J. Microbiol. 1996;34(1):23-29.

52 View
0 Download

Abstract: Since sequencing of randomly selected cDNA clones has been known to be a powerful approach to obtain information on gene expression pattern in specific cells or tissues, we have analyzed a 3'-directed cDNA library of vegetative mycelia of A. nidulans by single-pass sequencing of hundreds of randomly selected clones. Sequencing of 292 cDNA clones yielded 209 gene signatures (GSs) probably representing highly or lesser expressed genes in the vegetative mycelia. Among the 209 GSs, 25 (79 cDNA clones) appeared more than once and 184 only once. One GS appeared at a highest frequency of 6 times, 2 GSs5 times, 4 GSs 4 times, a GSs 3 times and 16 GSs twice. About 6.6% GSs comprizing of 13 GSs showed alternative polyadenylation. Among 23 redundant GSs, three were common in both mycelia and sexual organs, and 22 were probably mycelia-specific. Out of 209 GSs, 36 were identified in GenBank showing of 70% or greater similaritis. Only six GSs were for A. nidulans genes, and 13 GSs were of DNA or genes encoding cytoplasmic or organellar proteins. This pattern is similar to those in the human HepG2 cell line and in human colonic mucosa, although very few genes for nuclear proteins and for protein synthesis were in A. nidulans.

First
Prev
Page of 1
Next
Last

Search