Eukaryotic genomes contain numerous homologous repeat
sequences including redundant genes with divergent homology
that can be potential recombination targets. Recombination
between divergent sequences is rare but poses a substantial
threat to genome stability. The hexose transporter
(HXT) gene family shares high sequence similarities at both
protein and DNA levels, and some members are placed close
together in tandem arrays. In this study, we show that spontaneous
interstitial deletions occur at significantly high rates
in HXT gene clusters, resulting in chimeric HXT sequences
that contain a single junction point. We also observed that
DNA double-strand breaks created between HXT genes produce
primarily interstitial deletions, whereas internal cleavage
of the HXT gene resulted in gene conversions as well as deletion
products. Interestingly, interstitial deletions were less constrained
by sequence divergence than gene conversion. Moreover,
recombination-defective mutations differentially affected
the survival frequency. Mutations that impair single-strand
annealing (SSA) pathway greatly reduced the survival frequency
by 10–1,000-fold, whereas disruption of Rad51-dependent
homologous recombination exhibited only modest reduction.
Our results indicate that recombination in the tandemly
repeated HXT genes occurs primarily via SSA pathway.
Citations
Citations to this article as recorded by
Deletion of IRC19 Causes Defects in DNA Double-Strand Break Repair Pathways in Saccharomyces cerevisiae Ju-Hee Choi, Oyungoo Bayarmagnai, Sung-Ho Bae Journal of Microbiology.2024; 62(9): 749. CrossRef
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris Xiang Wang, Yi Li, Zhehao Jin, Xiangjian Liu, Xiang Gao, Shuyuan Guo, Tao Yu Synthetic and Systems Biotechnology.2023; 8(3): 445. CrossRef
Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms Jucan Gao, Cuifang Ye, Jintao Cheng, Lihong Jiang, Xinghao Yuan, Jiazhang Lian ACS Synthetic Biology.2022; 11(2): 547. CrossRef
Effects of the loss of mismatch repair genes on single-strand annealing between divergent sequences in Saccharomyces cerevisiae Ye-Seul Lim, Ju-Hee Choi, Kyu-Jin Ahn, Min-Ku Kim, Sung-Ho Bae Journal of Microbiology.2021; 59(4): 401. CrossRef
The increasing antibiotic resistance of Acinetobacter species
in both natural and hospital environments has become a serious
problem worldwide in recent decades. Because of both
intrinsic and acquired antimicrobial resistance (AMR) against
last-resort antibiotics such as carbapenems, novel therapeutics
are urgently required to treat Acinetobacter-associated infectious
diseases. Among the many pathogenic Acinetobacter
species, A. baumannii has been reported to be resistant to all
classes of antibiotics and contains many AMR genes, such as
blaADC (Acinetobacter-derived cephalosporinase). The AMR
of pathogenic Acinetobacter species is the result of several
different mechanisms, including active efflux pumps, mutations
in antibiotic targets, antibiotic modification, and low
antibiotic membrane permeability. To overcome the limitations
of existing drugs, combination theraphy that can increase
the activity of antibiotics should be considered in the
treatment of Acinetobacter infections. Understanding the
molecular mechanisms behind Acinetobacter AMR resistance
will provide vital information for drug development and
therapeutic strategies using combination treatment. Here,
we summarize the classic mechanisms of Acinetobacter AMR,
along with newly-discovered genetic AMR factors and currently
available antimicrobial adjuvants that can enhance drug
efficacy in the treatment of A. baumannii infections.
Citations
Citations to this article as recorded by
Disruption of bacterial interactions and community assembly in Babesia-infected Haemaphysalis longicornis following antibiotic treatment Myriam Kratou, Apolline Maitre, Lianet Abuin-Denis, Elianne Piloto-Sardiñas, Ivan Corona-Guerrero, Ana Laura Cano-Argüelles, Alejandra Wu-Chuang, Timothy Bamgbose, Consuelo Almazan, Juan Mosqueda, Dasiel Obregón, Lourdes Mateos-Hernández, Mourad Ben Said, BMC Microbiology.2024;[Epub] CrossRef
A 19-year longitudinal study to characterize carbapenem-nonsusceptible Acinetobacter isolated from patients with bloodstream infections and the contribution of conjugative plasmids to carbapenem resistance and virulence Pek Kee Chen, Yi-Tzu Lee, Chia-Ying Liu, Tran Thi Dieu Thuy, Kieu Anh, Jiunn-Jong Wu, Chun-Hsing Liao, Yu-Tsung Huang, Yu-Chen Chen, Cheng-Yen Kao Journal of Microbiology, Immunology and Infection.2024; 57(2): 288. CrossRef
MOLECULAR ANALYSIS OF THE MCR-1 GENE IN PSEUDOMONAS AERUGINOSA AND ACINETOBACTER BAUMANII STRAINS Ömer Akgül Ankara Universitesi Eczacilik Fakultesi Dergisi.2024; 48(3): 21. CrossRef
Effect of Phenylalanine–Arginine Beta-Naphthylamide on the Values of Minimum Inhibitory Concentration of Quinolones and Aminoglycosides in Clinical Isolates of Acinetobacter baumannii Stefany Plasencia-Rebata, Saul Levy-Blitchtein, Juana del Valle-Mendoza, Wilmer Silva-Caso, Isaac Peña-Tuesta, William Vicente Taboada, Fernando Barreda Bolaños, Miguel Angel Aguilar-Luis Antibiotics.2023; 12(6): 1071. CrossRef
A comprehensive genomic analysis provides insights on the high environmental adaptability of Acinetobacter strains Yang Zhao, Hua-Mei Wei, Jia-Li Yuan, Lian Xu, Ji-Quan Sun Frontiers in Microbiology.2023;[Epub] CrossRef
Acinetobacter baumannii in blood-borne and central nervous system infections in intensive care unit children: molecular and genetic characteristics and clinical significance Zulfirya Z. Sadeeva, Irina E. Novikova, Natalia M. Alyabyeva, Anna V. Lazareva, Tatiana M. Komyagina, Olga V. Karaseva, Marina G. Vershinina, Andrey P. Fisenko Russian Journal of Infection and Immunity.2023; 13(2): 289. CrossRef
Herbal Products and Their Active Constituents Used Alone and in Combination with Antibiotics against Multidrug-Resistant Bacteria Anna Herman, Andrzej P. Herman Planta Medica.2023; 89(02): 168. CrossRef
A Systematic Review of Culture-Based Methods for Monitoring Antibiotic-Resistant Acinetobacter, Aeromonas, and Pseudomonas as Environmentally Relevant Pathogens in Wastewater and Surface Water Erin G. Milligan, Jeanette Calarco, Benjamin C. Davis, Ishi M. Keenum, Krista Liguori, Amy Pruden, Valerie J. Harwood Current Environmental Health Reports.2023; 10(2): 154. CrossRef
Gold nanoparticle-DNA aptamer-assisted delivery of antimicrobial peptide effectively inhibits Acinetobacter baumannii infection in mice Jaeyeong Park, Eunkyoung Shin, Ji-Hyun Yeom, Younkyung Choi, Minju Joo, Minho Lee, Je Hyeong Kim, Jeehyeon Bae, Kangseok Lee Journal of Microbiology.2022; 60(1): 128. CrossRef
In vitro study to evaluate the antimicrobial activity of various multifunctional cosmetic ingredients and chlorphenesin on bacterial species at risk in the cosmetic industry Benjamin Youenou, Amandine Chauviat, Chrisse Ngari, Valérie Poulet, Sylvie Nazaret Journal of Applied Microbiology.2022; 132(2): 933. CrossRef
Profiles of Microbial Community and Antibiotic Resistome in Wild Tick Species Nana Wei, Jinmiao Lu, Yi Dong, Shibo Li, Jack A. Gilbert mSystems.2022;[Epub] CrossRef
Conventional and Real-Time PCR Targeting blaOXA Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Acinetobacter baumannii Clinical Strains Dagmara Depka, Agnieszka Mikucka, Tomasz Bogiel, Mateusz Rzepka, Patryk Zawadka, Eugenia Gospodarek-Komkowska Antibiotics.2022; 11(4): 455. CrossRef
In vitro synergistic activity of colistin and teicoplanin combination against multidrug-resistant Acinetobacter spp Osama Mohamed Samy Mohamed Rady, Laila El-Attar, Amira Amine The Journal of Antibiotics.2022; 75(3): 181. CrossRef
Biogenic silver nanoparticle (Bio‐AgNP) has an antibacterial effect against carbapenem‐resistant
Acinetobacter baumannii
with synergism and additivity when combined with polymyxin B
Suzane Olachea Allend, Marcelle Oliveira Garcia, Kamila Furtado da Cunha, Déborah Trota Farias de Albernaz, Mirian Elert da Silva, Rodrigo Yudi Ishikame, Luciano Aparecido Panagio, Gerson Nakazaro, Guilherme Fonseca Reis, Daniela Brayer Pereira, Daiane Dr Journal of Applied Microbiology.2022; 132(2): 1036. CrossRef
RapidResa Polymyxin Acinetobacter NP® Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii Maxime Bouvier, Mustafa Sadek, Stefano Pomponio, Fernando D’Emidio, Laurent Poirel, Patrice Nordmann Antibiotics.2021; 10(5): 558. CrossRef
Rapid detection of carbapenemase-producing Pseudomonas spp. using the NitroSpeed-Carba NP test Mustafa Sadek, Laurent Poirel, Patrice Nordmann Diagnostic Microbiology and Infectious Disease.2021; 99(3): 115280. CrossRef
Gain and loss of antibiotic resistant genes in multidrug resistant bacteria: One Health perspective Misung Kim, Jaeeun Park, Mingyeong Kang, Jihye Yang, Woojun Park Journal of Microbiology.2021; 59(6): 535. CrossRef
OXA-23 and OXA-40 producing carbapenem-resistant Acinetobacter baumannii in Central Illinois Janak Koirala, Isha Tyagi, Lohitha Guntupalli, Sameena Koirala, Udita Chapagain, Christopher Quarshie, Sami Akram, Vidya Sundareshan, Sajan Koirala, Jerry Lawhorn, Yohei Doi, Michael Olson Diagnostic Microbiology and Infectious Disease.2020; 97(1): 114999. CrossRef
Rapid Polymyxin/Pseudomonas NP test for rapid detection of polymyxin susceptibility/resistance in Pseudomonas aeruginosa Mustafa Sadek, Camille Tinguely, Laurent Poirel, Patrice Nordmann European Journal of Clinical Microbiology & Infectious Diseases.2020; 39(9): 1657. CrossRef
Stress responses linked to antimicrobial resistance in Acinetobacter species Bora Shin, Chulwoo Park, Woojun Park Applied Microbiology and Biotechnology.2020; 104(4): 1423. CrossRef
Carbapenemases: Transforming Acinetobacter baumannii into a Yet More Dangerous Menace Maria Soledad Ramirez, Robert A. Bonomo, Marcelo E. Tolmasky Biomolecules.2020; 10(5): 720. CrossRef
Pharmacokinetics, Safety, and Tolerability of Intravenous Durlobactam and Sulbactam in Subjects with Renal Impairment and Healthy Matched Control Subjects John O’Donnell, Richard A. Preston, Grigor Mamikonyan, Emily Stone, Robin Isaacs Antimicrobial Agents and Chemotherapy.2019;[Epub] CrossRef
Efficient Delivery of Antisense Oligonucleotides by an Amphipathic Cell-Penetrating Peptide in Acinetobacter baumannii Zhou Chen, Dan Nie, Yue Hu, Mingkai Li, Zheng Hou, Xinggang Mao, Xiaoxing Luo, Xiaoyan Xue Current Drug Delivery.2019; 16(8): 728. CrossRef
Restoring the activity of the antibiotic aztreonam using the polyphenol epigallocatechin gallate (EGCG) against multidrug-resistant clinical isolates of Pseudomonas aeruginosa
Jonathan W. Betts, Michael Hornsey, Paul G. Higgins, Kai Lucassen, Julia Wille, Francisco J. Salguero, Harald Seifert, Roberto M. La Ragione
Journal of Medical Microbiology
.2019; 68(10): 1552. CrossRef
Antibiotic-resistant clones in Gram-negative pathogens: presence of global clones in Korea Kwan Soo Ko Journal of Microbiology.2019; 57(3): 195. CrossRef
Alternative fate of glyoxylate during acetate and hexadecane metabolism in Acinetobacter oleivorans DR1 Chulwoo Park, Bora Shin, Woojun Park Scientific Reports.2019;[Epub] CrossRef
Nationwide surveillance of antimicrobial resistance among clinically important Gram-negative bacteria, with an emphasis on carbapenems and colistin: Results from the Surveillance of Multicenter Antimicrobial Resistance in Taiwan (SMART) in 2018 Yu-Lin Lee, Min-Chi Lu, Pei-Lan Shao, Po-Liang Lu, Yen-Hsu Chen, Shu-Hsing Cheng, Wen-Chien Ko, Chi-Ying Lin, Ting-Shu Wu, Muh-Yong Yen, Lih-Shinn Wang, Chang-Pan Liu, Wen-Sen Lee, Zhi-Yuan Shi, Yao-Shen Chen, Fu-Der Wang, Shu-Hui Tseng, Chao-Nan Lin, Yu- International Journal of Antimicrobial Agents.2019; 54(3): 318. CrossRef
The use of polymyxins to treat carbapenem resistant infections in neonates and children Reenu Thomas, Sithembiso Velaphi, Sally Ellis, A. Sarah Walker, Joseph F. Standing, Paul Heath, Mike Sharland, Daniele Dona’ Expert Opinion on Pharmacotherapy.2019; 20(4): 415. CrossRef
Plasmid-mediated mcr-1 gene in Acinetobacter baumannii and Pseudomonas aeruginosa: first report from Pakistan Fareeha Hameed, Muhammad Asif Khan, Hafsah Muhammad, Tahir Sarwar, Hazrat Bilal, Tayyab Ur Rehman Revista da Sociedade Brasileira de Medicina Tropical.2019;[Epub] CrossRef
Identification of factors needed by a clinical isolate of Acinetobacter baumannii to resist antibacterial compounds Celena M. Gwin, Natalia Prakash, Nathan W. Rigel BIOS.2019; 90(3): 149. CrossRef
A Resazurin Reduction-Based Assay for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii and Pseudomonas aeruginosa Mathilde Lescat, Laurent Poirel, Camille Tinguely, Patrice Nordmann, Nathan A. Ledeboer Journal of Clinical Microbiology.2019;[Epub] CrossRef
Expansion of antibacterial spectrum of xanthorrhizol against Gram-negatives in combination with PMBN and food-grade antimicrobials Man Su Kim, Ha-Rim Kim, Haebom Kim, Soo-Keun Choi, Chang-Hwan Kim, Jae-Kwan Hwang, Seung-Hwan Park Journal of Microbiology.2019; 57(5): 405. CrossRef
Carbapenem-resistant Acinetobacter baumannii in patients with burn injury: A systematic review and meta-analysis William Gustavo Lima, Geisa Cristina Silva Alves, Cristina Sanches, Simone Odília Antunes Fernandes, Magna Cristina de Paiva Burns.2019; 45(7): 1495. CrossRef
Performances of the Rapid Polymyxin Acinetobacter and Pseudomonas Tests for Colistin Susceptibility Testing Mathilde Lescat, Laurent Poirel, Aurélie Jayol, Patrice Nordmann Microbial Drug Resistance.2019; 25(4): 520. CrossRef
In vitro activities of ceftazidime/avibactam alone or in combination with antibiotics against multidrug-resistant Acinetobacter baumannii isolates Emel Mataracı Kara, Mesut Yılmaz, Berna Özbek Çelik Journal of Global Antimicrobial Resistance.2019; 17: 137. CrossRef
Zoonotic Diseases and Phytochemical Medicines for Microbial Infections in Veterinary Science: Current State and Future Perspective Bora Shin, Woojun Park Frontiers in Veterinary Science.2018;[Epub] CrossRef
A formidable foe: carbapenem-resistant Acinetobacter baumannii and emerging nonantibiotic therapies Richard R. Watkins Expert Review of Anti-infective Therapy.2018; 16(8): 591. CrossRef
Plasma and Intrapulmonary Concentrations of ETX2514 and Sulbactam following Intravenous Administration of ETX2514SUL to Healthy Adult Subjects Keith A. Rodvold, Mark H. Gotfried, Robin D. Isaacs, John P. O'Donnell, Emily Stone Antimicrobial Agents and Chemotherapy.2018;[Epub] CrossRef