Journal of Microbiology

Journal Article

Aeration Effects on Metabolic Events during Sporulation of Bacillus thuringiensis: Mohammad H. Sarrafzadeh , Sabine Schorr-Galindo , Hyun-Joon La , Hee-Mock Oh; J. Microbiol. 2014;52(7):597-603. Published online June 28, 2014; DOI: https://doi.org/10.1007/s12275-014-3547-9

78 View
0 Download
6 Crossref

The metabolism of Bacillus thuringiensis during its sporulation process was investigated under different concentrations of oxygen. At the beginning of sporulation, the aeration conditions were regulated to obtain different oxygen transfer rates (OTR) in four separate fermentations, representing interrupted, limited, non-limited, and saturated oxygenation, respectively. A higher OTR resulted in a higher pH, up to about 9 in the case of saturated oxygenation, while the interrupted oxygenation resulted in a significantly acidic culture. In contrast, the absence of oxygen resulted in rapid sporangia lysis and caused acidification of the medium, indicating a distinctly different sporangia composition and different metabolism. The bacterium also showed different CO2 production rates during sporulation, although amaximum point was observed in every case.With a higher OTR, the maximal value was observed after a longer time and at a lower value (40, 26, and 13 mmol/L/h for limited, non-limited, and saturated cases, respectively). Despite the exhaustion of glucose prior to the sporulation phase, the interrupted oxygenation resulted in acetate, lactate, and citrate in the medium with a maximum concentration of 4.8, 1.3, and 5.0 g/L, respectively. Notwithstanding, while the metabolic events differed visibly in the absence of oxygen, once sporulation was triggered, it was completed, even in the case of an interrupted oxygen supply.

Citations

Citations to this article as recorded by

High cell density cultivation of Bacillus subtilis NCIM 2063: Modeling, optimization and a scale-up procedure
Sandra Stamenkovic-Stojanovic, Ivana Karabegovic, Bojana Danilovic, Stojan Mancic, Miodrag Lazic
Journal of the Serbian Chemical Society.2023; 88(11): 1103. CrossRef
Effect of the volumetric oxygen mass transfer coefficient on producing δ-endotoxins by Bacillus thuringiensis in culture medium based on forage palm
Túlio Alexandre Freire da Silva, Lívia Santos de Freitas, Larita Veruska José Bezerra da Silva, José Manoel Wanderley Duarte Neto, Gilvanda Ribeiro da Silva, Liane Maria de Almeida Castro Maranhão, Cynthia Araújo de Lacerda, José de Paula Oliveira, Raquel
Biocatalysis and Agricultural Biotechnology.2021; 32: 101960. CrossRef
Review on biopesticide production by Bacillus thuringiensis subsp. kurstaki since 1990: Focus on bioprocess parameters
Wafa Jallouli, Fatma Driss, Luc Fillaudeau, Souad Rouis
Process Biochemistry.2020; 98: 224. CrossRef
Solid-state fermentation of Bacillus thuringiensis var kurstaki HD-73 maintains higher biomass and spore yields as compared to submerged fermentation using the same media
Jorge Lima-Pérez, Marcos López-Pérez, Gustavo Viniegra-González, Octavio Loera
Bioprocess and Biosystems Engineering.2019; 42(9): 1527. CrossRef
Modeling of Fermentation Process of Bacillus Thuringiensis as a Sporulating Bacterium
Soroush Soleymani, Mohammad-Hossein Sarrafzadeh, Navid Mostoufi
Chemical Product and Process Modeling.2019;[Epub] CrossRef
Optimal strategies for bioremediation of nitrate-contaminated groundwater and microalgae biomass production
Fariba Rezvani, Mohammad-Hossein Sarrafzadeh, Seong-Hyun Seo, Hee-Mock Oh
Environmental Science and Pollution Research.2018; 25(27): 27471. CrossRef

Research Support, Non-U.S. Gov't

NOTE] Construction and Characterisation of an Antifungal Recombinant Bacillus thuringiensis with an Expanded Host Spectrum: Qin Liu , Jong Yul Roh , Yong Wang , Jae Young Choi , Xue Ying Tao , Jae Su Kim , Yeon Ho Je; J. Microbiol. 2012;50(5):874-877. Published online November 4, 2012; DOI: https://doi.org/10.1007/s12275-012-2201-7

36 View
0 Download
6 Scopus

Abstract: A novel antifungal Bacillus thuringiensis strain 19–22, ssp. kurstaki (H3a3b3c), was characterised. This strain included cry1Aa, cry1Ab, cry1Ac, and cry1D, which have high insecticidal activities against lepidopteran larvae other than Spodoptera exigua. To expand the host spectrum, a cry1E gene whose product is active against S. exigua was introduced into the isolate. The transformant successfully expressed the Cry1E protein without any loss of its original antifungal activities. These results indicate that this recombinant strain exhibits dual activities and may be used as an integrated control agent to control plant diseases and insect pests.

Journal Article

Chitinase Production by Bacillus thuringiensis and Bacillus licheniformis: Their Potential in Antifungal Biocontrol: Eman Zakaria Gomaa; J. Microbiol. 2012;50(1):103-111. Published online February 27, 2012; DOI: https://doi.org/10.1007/s12275-012-1343-y

55 View
0 Download
108 Crossref

Abstract

Thirty bacterial strains were isolated from the rhizosphere of plants collected from Egypt and screened for production of chitinase enzymes. Bacillus thuringiensis NM101-19 and Bacillus licheniformis NM120-17 had the highest chitinolytic activities amongst those investigated. The production of chitinase by B. thuringiensis and B. licheniformis was optimized using colloidal chitin medium amended with 1.5% colloidal chitin, with casein as a nitrogen source, at 30°C after five days of incubation. An enhancement of chitinase production by the two species was observed by addition of sugar substances and dried fungal mats to the colloidal chitin media. The optimal conditions for chitinase activity by B. thuringiensis and B. licheniformis were at 40°C, pH 7.0 and pH 8.0, respectively. Na+, Mg2+, Cu2+, and Ca2+ caused enhancement of enzyme activities whereas they were markedly inhibited by Zn2+, Hg2+, and Ag+. In vitro, B. thuringiensis and B. licheniformis chitinases had potential for cell wall lysis of many phytopathogenic fungi tested. The addition of B. thuringiensis chitinase was more effective than that of B. licheniformis in increasing the germination of soybean seeds infected with various phytopathogenic fungi.

Citations

Citations to this article as recorded by

Antagonism of Bacillus velezensis ZGE166 Against the Pathogenic Fungi Causing Corm Rot Disease in Saffron (Crocus sativus L.)
Yingqiu Guo, Li Tian, Xinyu Zhu, Shu Liu, Lili Wang, Wankui Li
Microbial Ecology.2025;[Epub] CrossRef
Chitinase-binding gene BvCHiB enhances resistance of Arabidopsis to verticillium wilt through JA and SA pathways
Yujing Liu
Highlights in Science, Engineering and Technology.2025; 129: 213. CrossRef
Effect of Pseudomonas protegens EMM-1 Against Rhizopus oryzae in Interactions with Mexican Autochthonous Red Maize
Bruce Manuel Morales-Barron, Violeta Larios-Serrato, Yolanda Elizabeth Morales-García, Verónica Quintero-Hernández, Paulina Estrada-de los Santos, Jesús Muñoz-Rojas
Life.2025; 15(4): 554. CrossRef
Biocontrol Potential of Streptomyces sp. C2-4 against Ceratobasidium cereale Caused Sharp Eyespot in Wheat
Miju Jo, Sunmin An, Da Som Kim, Won-Jae Chi, Sook-Young Park
Research in Plant Disease.2025; 31(1): 125. CrossRef
Process optimisation for improved chitinase production from marine isolate Bacillus haynesii and bioethanol production with Saccharomyces cerevisiae
Vishnupriya Govindaraj, Dinesh Kumar Anandan, Se-Kwon Kim, Ritu Raval, Keyur Raval
Brazilian Journal of Microbiology.2025; 56(2): 835. CrossRef
The Potential of Beneficial Microbes for Sustainable Alternative Approaches to Control Phytopathogenic Diseases
Ramadan Bakr, Ali Abdelmoteleb, Vianey Mendez-Trujillo, Daniel Gonzalez-Mendoza, Omar Hewedy
Microbiology Research.2025; 16(5): 105. CrossRef
Evaluation of Bacillus isolates as a biological control agents against soilborne phytopathogenic fungi
Derya Maral-Gül, Rengin Eltem
International Microbiology.2024; 28(S1): 75. CrossRef
Chitinase: a potent biocatalyst and its diverse applications
Gaytri Mahajan, Vibhuti Sharma, Reena Gupta
Biocatalysis and Biotransformation.2024; 42(2): 85. CrossRef
Bacillus cereus sensu lato antimicrobial arsenal: An overview
Louis Morandini, Simon Caulier, Claude Bragard, Jacques Mahillon
Microbiological Research.2024; 283: 127697. CrossRef
Effective biological control of Lentil (Lens culinaris) Fusarium wilt and plant growth promotion through native Rhizobacteria
Javeria Ayub, Ayesha Tahir, Umer Iqbal, Huma Ayub, Muhammad Zeeshan Hyder, Amna Kiyani, Fauzia Yusuf Hafeez, Muhammad Kashif Ilyas, Abdul Ghafoor, Tayyaba Yasmin
Physiological and Molecular Plant Pathology.2024; 129: 102203. CrossRef
Bacillus endophytic strains control Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici in tomato cv. Perinha
Guilherme Caldieraro Viana, Leonardo Oliveira Médici, Marcia Soares Vidal, José Ivo Baldani
Brazilian Journal of Microbiology.2024; 55(4): 4019. CrossRef
Exploring chitin: novel pathways and structures as promising targets for biopesticides
Malkiet Kaur, Manju Nagpal, Gitika Arora Dhingra, Ankit Rathee
Zeitschrift für Naturforschung C.2024; 79(5-6): 125. CrossRef
Chitinolytic microorganisms for biological control of plant pathogens: A Comprehensive review and meta-analysis
Sristi Das, Suvasri Dutta, Saibal Ghosh, Abhishek Mukherjee
Crop Protection.2024; 185: 106888. CrossRef
Improved Expression of a Thermostable GH18 Bacterial Chitinase in Two Different Escherichia coli Strains and Its Potential Use in Plant Protection and Biocontrol of Phytopathogenic Fungi
Aymen Ezzine, Safa Ben Hadj Mohamed, Sofiane Bezzine, Yosra Aoudi, Mohamed Rabeh Hajlaoui, Laura Baciou, Issam Smaali
Molecular Biotechnology.2024; 66(9): 2635. CrossRef
A highly active Chitinase-A of Serratia ficaria isolated from Pieris brassicae (Lepidoptera: Pieridae)
Merve Almula Bakirdogen, Gozde Busra Eroglu
Crop Protection.2024; 179: 106623. CrossRef
Bacillus genus industrial applications and innovation: First steps towards a circular bioeconomy
Leonardo Wedderhoff Herrmann, Luiz Alberto Junior Letti, Rafaela de Oliveira Penha, Vanete Thomaz Soccol, Cristine Rodrigues, Carlos Ricardo Soccol
Biotechnology Advances.2024; 70: 108300. CrossRef
In Vitro Antagonistic Activity of Plant Growth Promoting Rhizobacteria Against Aggressive Biotypes of the Green Mold
Baran Mis, Kemal Karaca, Rengin Eltem
Journal of Basic Microbiology.2024;[Epub] CrossRef
Flue-cured tobacco intercropping with insectary floral plants improves rhizosphere soil microbial communities and chemical properties of flue-cured tobacco
Jin Zhong, Wenze Pan, Shenglan Jiang, Yanxia Hu, Guangyuan Yang, Ke Zhang, Zhenyuan Xia, Bin Chen
BMC Microbiology.2024;[Epub] CrossRef
Insights into the whole genome sequence of Bacillus thuringiensis NBAIR BtPl, a strain toxic to the melon fruit fly, Zeugodacus cucurbitae
Nekkanti Aarthi, Vinod K. Dubey, Arakalagud N. Shylesha, Aditya Kukreti, Jagadeesh Patil, Keerthi M. Chandrashekara, Kandan Aravindaram, Ruqiya Seegenahalli, Nanditha Shivakumar, Manjunatha Channappa
Current Genetics.2024;[Epub] CrossRef
Marine chitinase AfChi: green defense management against Colletotrichum gloeosporioides and anthracnose
Rajesh K.M., Keyur Raval, Ritu Raval
AMB Express.2024;[Epub] CrossRef
Biocontrol of fungal phytopathogens by Bacillus pumilus
Jakub Dobrzyński, Zuzanna Jakubowska, Iryna Kulkova, Paweł Kowalczyk, Karol Kramkowski
Frontiers in Microbiology.2023;[Epub] CrossRef
PENGARUH pH, SUHU DAN JENIS SUBSTRAT TERHADAP AKTIVITAS KITINASE Bacillus sp. RNT9
Satrio Adil Pamungkas, Indun Dewi Puspita, Ustadi Ustadi
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology.2023; 19(1): 29. CrossRef
The Prospect of Hydrolytic Enzymes from Bacillus Species in the Biological Control of Pests and Diseases in Forest and Fruit Tree Production
Henry B. Ajuna, Hyo-In Lim, Jae-Hyun Moon, Sang-Jae Won, Vantha Choub, Su-In Choi, Ju-Yeol Yun, Young Sang Ahn
International Journal of Molecular Sciences.2023; 24(23): 16889. CrossRef
Characterization of chitinolytic bacteria newly isolated from the termite Microcerotermes sp. and their biocontrol potential against plant pathogenic fungi
Kittipong Chanworawit, Pachara Wangsoonthorn, Pinsurang Deevong
Bioscience, Biotechnology, and Biochemistry.2023; 87(9): 1077. CrossRef
Enhanced production of N-acetyl-glucosaminidase by marine Aeromonas caviae CHZ306 in bioreactor
Flávio Augusto Cardozo, Valker Feitosa, Carlos Miguel Nóbrega Mendonça, Francisco Vitor Santos da Silva, Attilio Converti, Ricardo Pinheiro de Souza Oliveira, Adalberto Pessoa
Brazilian Journal of Microbiology.2023; 54(3): 1533. CrossRef
Gas Chromatography–Mass Spectrometry Profiling of Volatile Metabolites Produced by Some Bacillus spp. and Evaluation of Their Antibacterial and Antibiotic Activities
Moldir Koilybayeva, Zhanserik Shynykul, Gulbaram Ustenova, Krzysztof Waleron, Joanna Jońca, Kamilya Mustafina, Akerke Amirkhanova, Yekaterina Koloskova, Raushan Bayaliyeva, Tamila Akhayeva, Mereke Alimzhanova, Aknur Turgumbayeva, Gulden Kurmangaliyeva, Ai
Molecules.2023; 28(22): 7556. CrossRef
Thermostable Chitinase Producing Bacterium from Ijen Hot Spring – Indonesia: Isolation, Identification, and Characterization
Ruth Chrisnasari, Liony Priscilla Sutanto, Dian Paulina, Alicia Wahjudi, Tjandra Pantjajani, R.H. Setyobudi, A. Yaro, I. Zekker, M. Zahoor, T. Turkadze
E3S Web of Conferences.2023; 374: 00032. CrossRef
Isolation, biochemical characterization, and genome sequencing of two high‐quality genomes of a novel chitinolytic Jeongeupia species
Nathanael D. Arnold, Daniel Garbe, Thomas B. Brück
MicrobiologyOpen.2023;[Epub] CrossRef
Approach for quick exploration of highly effective broad-spectrum biocontrol strains based on PO8 protein inhibition
Mei Gu, Jiayun Fu, Honglin Yan, Xiaofeng Yue, Shancang Zhao, Qi Zhang, Peiwu Li
npj Science of Food.2023;[Epub] CrossRef
Antifungal Activity of Partially Purified Bacterial Chitinase Against Alternaria alternata
Neslihan Dikbaş, Sevda Uçar, Elif Tozlu, Merve Şenol Kotan, Recep Kotan
Erwerbs-Obstbau.2023; 65(4): 761. CrossRef
Characterization of the chemical fungicides-responsive and bacterial pathogen-preventing Bacillus licheniformis in rice spikelet
Chengfang Zhan, Mengchen Wu, Hongda Fang, Xiaoyu Liu, Jiuyue Pan, Xiaoyan Fan, Mengcen Wang, Haruna Matsumoto
Food Quality and Safety.2023;[Epub] CrossRef
Macrolactin A mediated biocontrol of Fusarium oxysporum and Rhizoctonia solani infestation on Amaranthus hypochondriacus by Bacillus subtilis BS-58
Chitra Pandey, Deepti Prabha, Yogesh Kumar Negi, Dinesh Kumar Maheshwari, Shrivardhan Dheeman, Monika Gupta
Frontiers in Microbiology.2023;[Epub] CrossRef
Formation of recombinant bifunctional fusion protein: A newer approach to combine the activities of two enzymes in a single protein
Patel Nilpa, Kapadia Chintan, R. Z. Sayyed, Hesham El Enshasy, Hala El Adawi, Alaa Alhazmi, Atiah H. Almalki, Shafiul Haque, Rahul Datta
PLOS ONE.2022; 17(4): e0265969. CrossRef
Efficiency and mechanisms of action of pelletized compost loaded with Bacillus velezensis CE 100 for controlling tomato Fusarium wilt
Seo Hyun Hwang, Chaw Ei Htwe Maung, Jun Su Noh, Woon Seon Baek, Jeong-Yong Cho, Kil Yong Kim
Biological Control.2022; 176: 105088. CrossRef
Purification, characterization, and antifungal activity of Bacillus cereus strain NK91 chitinase from rhizospheric soil samples of Himachal Pradesh, India
Nirja Thakur, Amarjit K Nath, Anjali Chauhan, Rakesh Gupta
Biotechnology and Applied Biochemistry.2022; 69(5): 1830. CrossRef
Macrolactin a Mediated Biocontrol of Two Important Fungal Pathogens of Amaranthus Hypochondriacus by Bacillus Subtilis BS-58
Chitra Pandey, Deepti Prabha, Yogesh Kumar Negi, D. K. Maheshwari
SSRN Electronic Journal.2022;[Epub] CrossRef
Morphological and structural characterization of chitin as a substrate for the screening, production, and molecular characterization of chitinase by Bacillus velezensis
Digvijay Dahiya, Akhil Pilli, Pratap Raja Reddy Chirra, Vinay Sreeramula, Nitish Venkateswarlu Mogili, Seenivasan Ayothiraman
Environmental Science and Pollution Research.2022; 29(57): 86550. CrossRef
Recent Advances and Technologies in Chitinase Production Under Solid-State Fermentation
Mini K. Paul, Umesh B.T, Jyothis Mathew
Biosciences Biotechnology Research Asia.2022; 19(4): 815. CrossRef
Effect of soil management systems on the rhizosphere bacterial community structure of tobacco: Continuous cropping vs. paddy-upland rotation
Peng Wang, Shen Yan, Wenshui Zhang, Xiaodan Xie, Mingjie Li, Tianbao Ren, Li Gu, Zhongyi Zhang
Frontiers in Plant Science.2022;[Epub] CrossRef
Broad-spectrum resistance mechanism of serine protease Sp1 in Bacillus licheniformis W10 via dual comparative transcriptome analysis
Lina Yang, Chun Yan, Shuai Peng, Lili Chen, Junjie Guo, Yihe Lu, Lianwei Li, Zhaolin Ji
Frontiers in Microbiology.2022;[Epub] CrossRef
Isolation of Chitinolytic Bacteria from European Sea Bass Gut Microbiota Fed Diets with Distinct Insect Meals
Fábio Rangel, Rafaela A. Santos, Marta Monteiro, Ana Sofia Lavrador, Laura Gasco, Francesco Gai, Aires Oliva-Teles, Paula Enes, Cláudia R. Serra
Biology.2022; 11(7): 964. CrossRef
Current Perspectives on Chitinolytic Enzymes and Their Agro-Industrial Applications
Vikram Poria, Anuj Rana, Arti Kumari, Jasneet Grewal, Kumar Pranaw, Surender Singh
Biology.2021; 10(12): 1319. CrossRef
Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats
Delfini CD, Villegas LB, Martínez MA, Baigorí MD
Current Microbiology.2021; 78(7): 2787. CrossRef
Bacilli as sources of agrobiotechnology: recent advances and future directions
Zerihun T. Dame, Mahfuz Rahman, Tofazzal Islam
Green Chemistry Letters and Reviews.2021; 14(2): 246. CrossRef
A Novel Antidipteran Bacillus thuringiensis Strain: Unusual Cry Toxin Genes in a Highly Dynamic Plasmid Environment
Nancy Fayad, Zakaria Kambris, Laure El Chamy, Jacques Mahillon, Mireille Kallassy Awad, Karyn N. Johnson
Applied and Environmental Microbiology.2021;[Epub] CrossRef
Quantitative single molecule RNA-FISH and RNase-free cell wall digestion in Neurospora crassa
Bradley M. Bartholomai, Amy S. Gladfelter, Jennifer J. Loros, Jay C. Dunlap
Fungal Genetics and Biology.2021; 156: 103615. CrossRef
Antifungal Activity of Bacillus velezensis CE 100 against Anthracnose Disease (Colletotrichum gloeosporioides) and Growth Promotion of Walnut (Juglans regia L.) Trees
Vantha Choub, Henry B. Ajuna, Sang-Jae Won, Jae-Hyun Moon, Su-In Choi, Chaw Ei Htwe Maung, Chul-Woo Kim, Young Sang Ahn
International Journal of Molecular Sciences.2021; 22(19): 10438. CrossRef
Key parameters optimization of chitosan production from Aspergillus terreus using apple waste extract as sole carbon source
Alireza Habibi, Salar Karami, Kambiz Varmira, Malihe Hadadi
Bioprocess and Biosystems Engineering.2021; 44(2): 283. CrossRef
Chitinases production: A robust enzyme and its industrial applications
Rahul Vikram Singh, Krishika Sambyal, Anjali Negi, Shubham Sonwani, Ritika Mahajan
Biocatalysis and Biotransformation.2021; 39(3): 161. CrossRef
Microbial chitinases: properties, enhancement and potential applications
Eman Zakaria Gomaa
Protoplasma.2021; 258(4): 695. CrossRef
Drought Exposed Burkholderia seminalis JRBHU6 Exhibits Antimicrobial Potential Through Pyrazine-1,4-Dione Derivatives Targeting Multiple Bacterial and Fungal Proteins
Jay Kishor Prasad, Priyanka Pandey, Richa Anand, Richa Raghuwanshi
Frontiers in Microbiology.2021;[Epub] CrossRef
Pectin lyase enhances cotton resistance to Verticillium wilt by inducing cell apoptosis of Verticillium dahliae
Jing Zhang, Xinru Yu, Chaojun Zhang, Qiong Zhang, Ying Sun, Heqin Zhu, Canming Tang
Journal of Hazardous Materials.2021; 404: 124029. CrossRef
Bacillus licheniformis PR2 Controls Fungal Diseases and Increases Production of Jujube Fruit under Field Conditions
Jun-Hyeok Kwon, Sang-Jae Won, Jae-Hyun Moon, Uk Lee, Yun-Serk Park, Chaw Ei Htwe Maung, Henry B. Ajuna, Young Sang Ahn
Horticulturae.2021; 7(3): 49. CrossRef
Bacillus subtilis and B. licheniformis Isolated from Heterorhabditis indica Infected Apple Root Borer (Dorysthenes huegelii) Suppresses Nematode Production in Galleria mellonella
Akanksha Upadhyay, Sharad Mohan
Acta Parasitologica.2021; 66(3): 989. CrossRef
Dissecting the Environmental Consequences of Bacillus thuringiensis Application for Natural Ecosystems
Maria E. Belousova, Yury V. Malovichko, Anton E. Shikov, Anton A. Nizhnikov, Kirill S. Antonets
Toxins.2021; 13(5): 355. CrossRef
Current Insights on Vegetative Insecticidal Proteins (Vip) as Next Generation Pest Killers
Tahira Syed, Muhammad Askari, Zhigang Meng, Yanyan Li, Muhammad Abid, Yunxiao Wei, Sandui Guo, Chengzhen Liang, Rui Zhang
Toxins.2020; 12(8): 522. CrossRef
Comparative bioefficacy of Bacillus and Pseudomonas chitinase against Helopeltis theivora in tea (Camellia sinensis (L.) O.Kuntze
M. Suganthi, S. Arvinth, P. Senthilkumar
Physiology and Molecular Biology of Plants.2020; 26(10): 2053. CrossRef
Curing piglets from diarrhea and preparation of a healthy microbiome with Bacillus treatment for industrial animal breeding
Shousong Yue, Zhentian Li, Fuli Hu, Jean-François Picimbon
Scientific Reports.2020;[Epub] CrossRef
Bacillus Thuringiensis – Nowy PotencjaŁ Aplikacyjny
Aleksandra Gęsicka, Agata Henschke, Zuzanna Barańska, Agnieszka Wolna-Maruwka
Postępy Mikrobiologii - Advancements of Microbiology.2020; 59(4): 357. CrossRef
Bioconversion of Colloidal Chitin Using Novel Chitinase from Glutamicibacter uratoxydans Exhibiting Anti-fungal Potential by Hydrolyzing Chitin Within Fungal Cell Wall
Tayyaba Asif, Urooj Javed, Syeda Bushra Zafar, Asma Ansari, Shah Ali Ul Qader, Afsheen Aman
Waste and Biomass Valorization.2020; 11(8): 4129. CrossRef
Biocontrol potential of chitinases produced by newly isolated Chitinophaga sp. S167
Sonia Sharma, Shiv Kumar, Anjali Khajuria, Puja Ohri, Rajinder Kaur, Ramandeep Kaur
World Journal of Microbiology and Biotechnology.2020;[Epub] CrossRef
Screening of an Alkaline CMCase-Producing Strain and the Optimization of its Fermentation Condition
Junmei Zhou, Lianghong Yin, Chenbin Wu, Sijia Wu, Jidong Lu, Hailing Fang, Yongchang Qian
Current Pharmaceutical Biotechnology.2020; 21(13): 1304. CrossRef
Screening of Bacillus thuringiensis strains to identify new potential biocontrol agents against Sclerotinia sclerotiorum and Plutella xylostella in Brassica campestris L.
Meiling Wang, Lili Geng, Xiaoxiao Sun, Changlong Shu, Fuping Song, Jie Zhang
Biological Control.2020; 145: 104262. CrossRef
Chitinases of Bacillus thuringiensis: Phylogeny, Modular Structure, and Applied Potentials
Sheila A. Martínez-Zavala, Uriel E. Barboza-Pérez, Gustavo Hernández-Guzmán, Dennis K. Bideshi, José E. Barboza-Corona
Frontiers in Microbiology.2020;[Epub] CrossRef
Isolation and Identification of Plant Growth-Promoting Bacteria Highly Effective in Suppressing Root Rot in Fava Beans
Mona M. G. Saad, Mahrous Kandil, Youssef M. M. Mohammed
Current Microbiology.2020; 77(9): 2155. CrossRef
Accelerating the Morphogenetic Cycle of the Viral Vector Aedes aegypti Larvae for Faster Larvicidal Bioassays
José Domingos Fontana, Rafael Lopes Ferreira, Tatiana Zuccolotto, Cibelle de Borba Dallagassa, Leonardo Pellizzari Wielewski, Barbara Maria Santano Chalcoski, Mario Antonio Navarro da Silva, Vinicius Sobrinho Richardi, Jonas Golart, Cynara de Melo Rodoval
BioMed Research International.2020;[Epub] CrossRef
Isolation of a novel rhizobacteria having multiple plant growth promoting traits and antifungal activity against certain phytopathogens
Madhurankhi Goswami, Suresh Deka
Microbiological Research.2020; 240: 126516. CrossRef
Long-term continuously monocropped peanut significantly changed the abundance and composition of soil bacterial communities
Mingna Chen, Hu Liu, Shanlin Yu, Mian Wang, Lijuan Pan, Na Chen, Tong Wang, Xiaoyuan Chi, Binghai Du
PeerJ.2020; 8: e9024. CrossRef
Lipopeptide mediated biocontrol activity of endophytic Bacillus subtilis against fungal phytopathogens
Dibya Jyoti Hazarika, Gunajit Goswami, Trishnamoni Gautom, Assma Parveen, Pompi Das, Madhumita Barooah, Robin Chandra Boro
BMC Microbiology.2019;[Epub] CrossRef
Microbial metabolomics: essential definitions and the importance of cultivation conditions for utilizing Bacillus species as bionematicides
I. Horak, G. Engelbrecht, P.J. Jansen Rensburg, S. Claassens
Journal of Applied Microbiology.2019; 127(2): 326. CrossRef
Chitinases As The Key To The Interaction Between Plants And Microorganisms
Anna Kisiel, Katarzyna Jęckowska
Postępy Mikrobiologii - Advancements of Microbiology.2019; 58(3): 317. CrossRef
Biochemical characterization of chitinase A from Bacillus licheniformis DSM8785 expressed in Pichia pastoris KM71H
Gheorghita Menghiu, Vasile Ostafe, Radivoje Prodanovic, Rainer Fischer, Raluca Ostafe
Protein Expression and Purification.2019; 154: 25. CrossRef
Overview of the Antimicrobial Compounds Produced by Members of the Bacillus subtilis Group
Simon Caulier, Catherine Nannan, Annika Gillis, Florent Licciardi, Claude Bragard, Jacques Mahillon
Frontiers in Microbiology.2019;[Epub] CrossRef
Repertoire of the Bacillus thuringiensis Virulence Factors Unrelated to Major Classes of Protein Toxins and Its Role in Specificity of Host-Pathogen Interactions
Yury V. Malovichko, Anton A. Nizhnikov, Kirill S. Antonets
Toxins.2019; 11(6): 347. CrossRef
Unraveling the Optimal Culture Condition for the Antifungal Activity and IAA Production of Phylloplane Serratia plymuthica
Siti Nur Aisyah, Jefri Maldoni, Irma Sulastri, Weni Suryati, Yuli Marlisa, Lissa Herliana, Lily Syukriani, Renfiyeni Renfiyeni, Jamsari Jamsari
Plant Pathology Journal.2019; 18(1): 31. CrossRef
Inhibitory effect and possible mechanism of a Pseudomonas strain QBA5 against gray mold on tomato leaves and fruits caused by Botrytis cinerea
Pan Gao, Jiaxing Qin, Delong Li, Shanyue Zhou, Ya-Wen He
PLOS ONE.2018; 13(1): e0190932. CrossRef
Chitinases—Potential Candidates for Enhanced Plant Resistance towards Fungal Pathogens
Manish Kumar, Amandeep Brar, Monika Yadav, Aakash Chawade, V. Vivekanand, Nidhi Pareek
Agriculture.2018; 8(7): 88. CrossRef
Improved antifungal activity of barley derived chitinase I gene that overexpress a 32 kDa recombinant chitinase in Escherichia coli host
Nida Toufiq, Bushra Tabassum, Muhammad Umar Bhatti, Anwar Khan, Muhammad Tariq, Naila Shahid, Idrees Ahmad Nasir, Tayyab Husnain
Brazilian Journal of Microbiology.2018; 49(2): 414. CrossRef
Isolation and Evaluation of New Antagonist Bacillus Strains for the Control of Pathogenic and Mycotoxigenic Fungi of Fig Orchards
Özlem Öztopuz, Gülseren Pekin, Ro Dong Park, Rengin Eltem
Applied Biochemistry and Biotechnology.2018; 186(3): 692. CrossRef
Microbial and viral chitinases: Attractive biopesticides for integrated pest management
Francesca Berini, Chen Katz, Nady Gruzdev, Morena Casartelli, Gianluca Tettamanti, Flavia Marinelli
Biotechnology Advances.2018; 36(3): 818. CrossRef
Improvement of Chitinase Production by Bacillus thuringiensis NM101-19 for Antifungal Biocontrol through Physical Mutation
E. Z. Gomaa, O. M. El-Mahdy
Microbiology.2018; 87(4): 472. CrossRef
Bacterial chitinases and their application in biotechnology
Anna Kisiel, Ewa Kępczyńska
Postępy Mikrobiologii - Advancements of Microbiology.2017; 56(3): 306. CrossRef
Combating Fusarium Infection Using Bacillus-Based Antimicrobials
Noor Khan, Maskit Maymon, Ann Hirsch
Microorganisms.2017; 5(4): 75. CrossRef
Screening and Characterisation of Chitinolytic Microorganisms with Potential to Control White Root Disease of Hevea brasiliensis
Nor Afiqah Maiden, Aizat Shamin Noran, Mohd Adi Faiz Ahmad Fauzi, Safiah Atan
Journal of Rubber Research.2017; 20(3): 182. CrossRef
Antifungal Screening of Bioprotective Isolates against Botrytis cinerea, Fusarium pallidoroseum and Fusarium moniliforme
Antoinette De Senna, Amanda Lathrop
Fermentation.2017; 3(4): 53. CrossRef
Simultaneous fermentative chitinase and β-1,3 glucanase production from Streptomyces philanthi RM-1-1-38 and their antifungal activity against rice sheath blight disease
Sawai Boukaew, Wanida Petlamul, Wasana Suyotha, Poonsuk Prasertsan
BioTechnologia.2017; 97(4): 271. CrossRef
Bacillus thuringiensis: a successful insecticide with new environmental features and tidings
Gholamreza Salehi Jouzani, Elena Valijanian, Reza Sharafi
Applied Microbiology and Biotechnology.2017; 101(7): 2691. CrossRef
Enhancement of Exochitinase Production by Bacillus licheniformis AT6 Strain and Improvement of N-Acetylglucosamine Production
Mohamed Amine Aounallah, Imen Ben Slimene-Debez, Kais Djebali, Dorra Gharbi, Majdi Hammami, Sana Azaiez, Ferid Limam, Olfa Tabbene
Applied Biochemistry and Biotechnology.2017; 181(2): 650. CrossRef
Chitinase from Pseudomonas fluorescens and its insecticidal activity against Helopeltis theivora
M. Suganthi, P. Senthilkumar, S. Arvinth, K. N. Chandrashekara
The Journal of General and Applied Microbiology.2017; 63(4): 222. CrossRef
Silver nanoparticles as an antimicrobial agent: A case study on Staphylococcus aureus and Escherichia coli as models for Gram-positive and Gram-negative bacteria
Eman Zakaria Gomaa
The Journal of General and Applied Microbiology.2017; 63(1): 36. CrossRef
Optimised production of chitinase from a novel mangrove isolate, Bacillus pumilus MCB-7 using response surface methodology
K.S. Rishad, Sharrel Rebello, Vinod Kumar Nathan, S. Shabanamol, M.S. Jisha
Biocatalysis and Agricultural Biotechnology.2016; 5: 143. CrossRef
A new chitinase-D from a plant growth promoting Serratia marcescens GPS5 for enzymatic conversion of chitin
Papa Rao Vaikuntapu, Samudrala Rambabu, Jogi Madhuprakash, Appa Rao Podile
Bioresource Technology.2016; 220: 200. CrossRef
Bacillus thuringiensis C25 which is rich in cell wall degrading enzymes efficiently controls lettuce drop caused by Sclerotinia minor
Anupama Shrestha, Razia Sultana, Jong-Chan Chae, Kangmin Kim, Kui-Jae Lee
European Journal of Plant Pathology.2015; 142(3): 577. CrossRef
Isolation of a Chitinolytic Bacillus licheniformis S213 Strain Exerting a Biological Control Against Phoma medicaginis Infection
Imen Ben Slimene, Olfa Tabbene, Dorra Gharbi, Bacem Mnasri, Jean Marie Schmitter, Maria-Camino Urdaci, Ferid Limam
Applied Biochemistry and Biotechnology.2015; 175(7): 3494. CrossRef
Characterization of regulatory regions involved in the inducible expression of chiB in Bacillus thuringiensis
Chi-Chu Xie, Jin Shi, Hai-Yun Jia, Peng-Fei Li, Yang Luo, Jun Cai, Yue-Hua Chen
Archives of Microbiology.2015; 197(1): 53. CrossRef
YvoA and CcpA Repress the Expression of chiB in Bacillus thuringiensis
Kun Jiang, Li-na Li, Jin-hua Pan, Ting-ting Wang, Yue-hua Chen, Jun Cai, S.-J. Liu
Applied and Environmental Microbiology.2015; 81(19): 6548. CrossRef
Ecology of Bacillaceae
Ines Mandic-Mulec, Polonca Stefanic, Jan Dirk van Elsas, Patrick Eichenberger, Adam Driks
Microbiology Spectrum.2015;[Epub] CrossRef
Chitinase biotechnology: Production, purification, and application
Yuriy Mihaylov Stoykov, Atanas Ivanov Pavlov, Albert Ivanov Krastanov
Engineering in Life Sciences.2015; 15(1): 30. CrossRef
Efficient biosynthesis of a chitinase from Halobacterium salinarum expressed in Escherichia coli
Fatima Moscoso, Myriam Sieira, Alberto Domínguez, Francisco J. Deive, Maria A. Longo, Maria A. Sanromán
Journal of Chemical Technology & Biotechnology.2014; 89(11): 1653. CrossRef
Potential use and mode of action of the new strainBacillus thuringiensisUM96 for the biological control of the grey mould phytopathogenBotrytis cinerea
Sofía Martínez-Absalón, Daniel Rojas-Solís, Rocío Hernández-León, Cristina Prieto-Barajas, Ma. del Carmen Orozco-Mosqueda, Juan José Peña-Cabriales, Shohei Sakuda, Eduardo Valencia-Cantero, Gustavo Santoyo
Biocontrol Science and Technology.2014; 24(12): 1349. CrossRef
Isolation and characterization of an antifungal protein from Bacillus licheniformis HS10
Zhixin Wang, Yunpeng Wang, Li Zheng, Xiaona Yang, Hongxia Liu, Jianhua Guo
Biochemical and Biophysical Research Communications.2014; 454(1): 48. CrossRef
Dual silencing of long and short Amblyomma americanum acidic chitinase forms weakens the tick cement cone stability
Tae K. Kim, Jenny Curran, Albert Mulenga
Journal of Experimental Biology.2014;[Epub] CrossRef
Partial Purification of Bacterial Chitinase as Biocontrol of Leaf Blight Disease on Oil Palm
Muhammad Asril, Nisa Rachmania Mubarik, Aris Tri Wahyudi
Research Journal of Microbiology.2014; 9(6): 265. CrossRef
Characterization and evaluation of Staphylococcus sp. strain LZ16 for the biological control of rice blast caused by Magnaporthe oryzae
Qin Yu, Zhu Liu, Derun Lin, Wei Zhang, Qun Sun, Jianqing Zhu, Min Lin
Biological Control.2013; 65(3): 338. CrossRef
Development of an Industrial Microbial System for Chitinolytic Enzymes Production
F. Moscoso, L. Ferreira, M.A. Fernández de Dios, F.J. Deive, M.A. Longo, M.A. Sanromán
Industrial & Engineering Chemistry Research.2013; 52(30): 10046. CrossRef
Bacillus thuringiensiscolonises plant roots in a phylogeny-dependent manner
J. Cristian Vidal-Quist, Hilary J. Rogers, Eshwar Mahenthiralingam, Colin Berry
FEMS Microbiology Ecology.2013; 86(3): 474. CrossRef
Comparative Genome Analysis of Enterobacter cloacae
Wing-Yee Liu, Chi-Fat Wong, Karl Ming-Kar Chung, Jing-Wei Jiang, Frederick Chi-Ching Leung, Jingfa Xiao
PLoS ONE.2013; 8(9): e74487. CrossRef
Antifungal activity of the lipopeptides produced by Bacillus amyloliquefaciens anti-CA against Candida albicans isolated from clinic
Bo Song, Yan-Jun Rong, Ming-Xin Zhao, Zhen-Ming Chi
Applied Microbiology and Biotechnology.2013; 97(16): 7141. CrossRef

Research Support, Non-U.S. Gov'ts

Cloning and Molecular Characterization of a Novel Rolling-Circle Replicating Plasmid, pK1S-1, from Bacillus thuringiensis subsp. kurstaki K1: Ming Shun Li , Jong Yul Roh , Xueying Tao , Zi Niu Yu , Zi Duo Liu , Qin Liu , Hong Guang Xu , Hee Jin Shim , Yang-Su Kim , Yong Wang , Jae Young Choi , Yeon Ho Je; J. Microbiol. 2009;47(4):466-472. Published online September 9, 2009; DOI: https://doi.org/10.1007/s12275-009-0020-2

56 View
0 Download
4 Scopus

Abstract: Bacillus thuringiensis, an entomopathogenic bacterium belonging to the B. cereus group, harbors numerous extra-chromosomal DNA molecules whose sizes range from 2 to 250 kb. In this study, we used a plasmid capture system (PCS) to clone three small plasmids from B. thuringiensis subsp. kurstaki K1 which were not found in B. thuringiensis subsp. kurstaki HD-1, and determined the complete nucleotide sequence of plasmid pK1S-1 (5.5 kb). Of the six putative open reading frames (ORF2-ORF7) in pK1S-1, ORF2 (MobK1) showed approximately 90% aa identity with the Mob-proteins of pGI2 and pTX14-2, which are rolling circle replicating group VII (RCR group VII) plasmids from B. thuringiensis. In addition, a putative origin of transfer (oriT) showed 95.8% identity with those of pGI2 and pTX14-2. ORF3 (RepK1) showed relatively low aa identity (17.8~25.2%) with the Rep protein coded by RCR plasmids, however. The putative double- strand origin of replication (dso) and single-strand origin of replication (sso) of pK1S-1 exhibited approximately 70% and 64% identities with those of pGI2 and pTX14-2. ORF6 and 7 showed greater than 50% similarities with alkaline serine protease, which belongs to the subtilase family. The other 2 ORFs were identified as hypothetical proteins. To determine the replicon of pK1S-1, seven subclones were contructed in the B. thuringiensis ori-negative pHT1K vector and were electroporated into a plasmid cured B. thuringiensis strain. The 1.6 kb region that included the putative ORF3 (Rep1K), dso and ORF4, exhibited replication ability. These findings identified pK1S-1 as a new RCR group VII plasmid, and determined its replication region.

A Highly Pathogenic Strain of Bacillus thuringiensis serovar kurstaki in Lepidopteran Pests: Hatice Kati , Kazim Sezen , Remziye Nalcacioglu , Zihni Demirbag; J. Microbiol. 2007;45(6):553-557.; DOI: https://doi.org/2609 [pii]

50 View
0 Download

Abstract: In order to detect and identify the most toxic Bacillus thuringiensis strains against pests, we isolated a B. thuringiensis strain (Bn1) from Balaninus nucum (Coleoptera: Curculionidae), the most damaging hazelnut pest. Bn1 was characterized via morphological, biochemical, and molecular techniques. The isolate was serotyped, and the results showed that Bn1 was the B. thuringiensis serovar, kurstaki (H3abc). The scanning electron microscopy indicated that Bn1 has crystals with cubic and bipyramidal shapes. The Polymerase Chain Reactions (PCRs) revealed the presence of the cry1 and cry2 genes. The presence of Cry1 and Cry2 proteins in the Bn1 isolate was confirmed via SDS-PAGE, at approximately 130 kDa and 65 kDa, respectively. The bioassays conducted to determine the insecticidal activity of the Bn1 isolate were conducted with four distinct insects, using spore-crystal mixtures. We noted that Bn1 has higher toxicity as compared with the standard B. thuringiensis subsp. kurstaki (HD-1). The highest observed mortality was 90% against Malacosoma neustria and Lymantria dispar larvae. Our results show that the B. thuringiensis isolate (Bn1) may prove valuable as a significant microbial control agent against lepidopteran pests.

Expression of a Recombinant Cry1Ac Crystal Protein Fused with a Green Fluorescent Protein in Bacillus thuringiensis subsp. kurstaki Cry-B: Jong Yul Roh , In Hee Lee , Ming Shun Li , Jin Hee Chang , Jae Young Choi , Kyung Saeng Boo , Yeon Ho Je; J. Microbiol. 2004;42(4):340-345.; DOI: https://doi.org/2101 [pii]

52 View
0 Download

Abstract: To investigate the co-expression and crystallization of a fusion gene between the Bacillus thuringiensis crystal protein and a foreign protein in B. thuringiensis, the expression of the Cry1Ac fused with green fluorescent protein (GFP) genes in a B. thuringiensis Cry-B strain was examined. The cry1Ac gene was cloned in the B. thuringiensis-E. coli shuttle vector, pHT3101, under the control of the native cry1Ac gene promoter, while the GFP gene was inserted into the XhoI site upstream of the proteolytic cleavage site, in the middle region of the cry1Ac gene (pProAc-GFP). The B. thuringiensis Cry-B strain carrying pProAc-GFP (ProAc-GFP/CB) did not produce any inclusion bodies. However, the transformed strain expressed fusion protein forms although the expression level was relatively low. Furthermore, an immunoblot analysis using GFP and Cry1Ac antibodies showed that the fusion protein was not a single species, but rather multiple forms. In addition, the N-terminal fragment of Cry1Ac and a non-fused GFP were also found in the B. thuringiensis Cry-B strain after autolysis. The sporulated cells before autolysis and the spore-crystal mixture after autolysis of ProAc-GFP/CB exhibited insecticidal activities against Plutella xylostella larvae. Accordingly, the current results suggest that a fusion crystal protein produced by the transfomant, ProAc-GFP/CB, can be functionally expressed but easily degraded in B. thuringiensis.

Biosynthesis of Poly-β-Hydrozyalkanoates by Bacillus thuringiensis R-510: Lee, Kang Tae , Kim, Jeong Yoon , Rhee, young Ha , Bae, Kyung Sook , Kim, Young Baek; J. Microbiol. 1995;33(1):59-65.

69 View
0 Download

Abstract: Synthesis and accumulation of Poly-β-Hydrozyalkanoates (PHA) in Bacillus thuringiensis R-510 isolated from soil were investigatd. This organism was resistant to relatively higj concentration of propionate and had a capability of accumulatinf copolymers consisting of 3-hydroxybutyrate(3HB) and 3-hydroxyvalerate(3HV) when the medium was supplemented with propionate as a precursor, The PHA content maximally reached up to 44.5% of dry cell weight in the presence of 0.1% propionate. The molar fraction of 3HV in the copolymer was increased from 19.4 to 80,2 mol% by adding 0.05 to 0.5% propionate to glucose medium. The addition of propionate during exponential or stationary phase of cell growth was less effective for the enhancement of 3HV content in the copolymer, although cell mass and PHA content were not affected by the time of propionate addition. PHB homopolymer and copolymer produced by B. thuringiensis R-510 were measured to have number average molecular weights in the range of 53,000 to 65,000. Polydispersity indices were between 1.5 and 2.2. Some of the produced polymers had bimodal molecular weight distribution.

Biosynthesis of polyhydroxybutyrate and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by bacillus thuringiensis R-510: Park, Sang Kyu , Lee, Kang Tae , Kim, Young Baek , Rhee, Young Ha; J. Microbiol. 1997;35(2):127-133.

53 View
0 Download

Abstract: Biosynthesis of polyhydroxybutyrate and copolymer consisting of 3-hydroxybutyrate and 3-hydroxyvalerate [poly(3HB-co-3HV)] by Bacillus thuringiensis R-510 grown with glucose or with mixtures of glucose and propionate was investigated. n-Alkanoic acids other than propionate were not precursors of 3HV units. The fraction of 3HV unit in the copolymer increased from 0 to 84 mol% of 3HV. Polymer yield decreased as the fraction of propionate was increased but the molecular weight distribution was not affected by the composition of carbon substrate. The minimum melting temperature (around 65℃) of poly (3HB-co-3HV) copolymers was observed for the polymer bearing approximately 35 mol% of 3HV. Polyhydroxyalkanoates production by this organism was not dependent on nutritional limitation, but remarkably influenced by dissolved oxygen concentration in the culture medium. Low level of dissolved oxygen concentration prevented spore formation in the cells and stimulated the synthesis of polyhydroxyalkanoate. The composition of poly (3HB-co-3HV) produced by B. thuringiensis R-510 lyhydroxyalkanoate. The composition of poly(3HB-co-3HV) produced by B. thuringiensis R-510 varied according to the growth time. However, there was no evidence that polymers isolated from cells were mixtures of immiscible polymers.

First
Prev
Page of 1
Next
Last

Search