NOTE] Construction and Characterisation of an Antifungal Recombinant Bacillus thuringiensis with an Expanded Host Spectrum

Qin Liu; Jong Yul Roh; Yong Wang; Jae Young Choi; Xue Ying Tao; Jae Su Kim; Yeon Ho Je

doi:10.1007/s12275-012-2201-7

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NOTE] Construction and Characterisation of an Antifungal Recombinant Bacillus thuringiensis with an Expanded Host Spectrum: Qin Liu ¹, Jong Yul Roh ², Yong Wang ³, Jae Young Choi ¹, Xue Ying Tao ¹, Jae Su Kim ⁴, Yeon Ho Je ^1,5; Journal of Microbiology 2012;50(5):874-877
DOI: https://doi.org/10.1007/s12275-012-2201-7
Published online: November 4, 2012

¹Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul 151-742, Republic of Korea, ²Division of Medical Entomology, Korea National Institute of Health, Chungbuk 363-951, Republic of Korea, ³School of Life Science, Lanzhou University, 99 Dong Ganxi Road, Lanzhou 730000, P. R. China, ⁴Department of Agricultural Biology, College of Agricultural Life Science, Chonbuk National University, Jeonju 561-756, Republic of Korea, ⁵Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea¹Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul 151-742, Republic of Korea, ²Division of Medical Entomology, Korea National Institute of Health, Chungbuk 363-951, Republic of Korea, ³School of Life Science, Lanzhou University, 99 Dong Ganxi Road, Lanzhou 730000, P. R. China, ⁴Department of Agricultural Biology, College of Agricultural Life Science, Chonbuk National University, Jeonju 561-756, Republic of Korea, ⁵Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea

Corresponding author: Yeon Ho Je , Tel: +82-2-880-4706,

Received: 16 April 2012 • Accepted: 20 July 2012

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Abstract

A novel antifungal Bacillus thuringiensis strain 19–22, ssp. kurstaki (H3a3b3c), was characterised. This strain included cry1Aa, cry1Ab, cry1Ac, and cry1D, which have high insecticidal activities against lepidopteran larvae other than Spodoptera exigua. To expand the host spectrum, a cry1E gene whose product is active against S. exigua was introduced into the isolate. The transformant successfully expressed the Cry1E protein without any loss of its original antifungal activities. These results indicate that this recombinant strain exhibits dual activities and may be used as an integrated control agent to control plant diseases and insect pests.

Keywords: Bacillus thuringiensis; antifungal activity; cry1E; insecticidal activity

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NOTE] Construction and Characterisation of an Antifungal Recombinant Bacillus thuringiensis with an Expanded Host Spectrum

J. Microbiol. 2012;50(5):874-877. Published online November 4, 2012

DOI: https://doi.org/10.1007/s12275-012-2201-7

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