Journal of Microbiology

Journal Article

Characterization and Comparative Genomic Analysis of vB_BceM_CEP1: A Novel Temperate Bacteriophage Infecting Burkholderia cepacia Complex: Momen Askoura, Eslam K Fahmy, Safya E Esmaeel, Wael A H Hegazy, Aliaa Abdelghafar; J. Microbiol. 2024;62(11):1035-1055. Published online November 18, 2024; DOI: https://doi.org/10.1007/s12275-024-00185-2

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The increasing prevalence of multidrug-resistant bacteria imminently threatens public health and jeopardizes nearly all aspects of modern medicine. The Burkholderia cepacia complex (Bcc) comprises Burkholderia cepacia and the related species of Gram-negative bacteria. Members of the Bcc group are opportunistic pathogens responsible for various chronic illnesses, including cystic fibrosis and chronic granulomatous disease. Phage therapy is emerging as a potential solution to combat the antimicrobial resistance crisis. In this study, a temperate phage vB_BceM_CEP1 was isolated from sewage and fully characterized. Transmission electron microscopy indicated that vB_BceM_CEP1 belongs to the family Peduoviridae. The isolated phage demonstrated enhanced environmental stability and antibiofilm potential. One-step growth analysis revealed a latent period of 30 min and an average burst size of 139 plaque-forming units per cell. The genome of vB_BceM_CEP1 consists of 32,486 bp with a GC content of 62.05%. A total of 40 open reading frames were annotated in the phage genome, and none of the predicted genes was annotated as tRNA. Notably, genes associated with antibiotic resistance, host virulence factors, and toxins were absent from the vB_BceM_CEP1 genome. Based on its unique phenotype and phylogeny, the isolated phage vB_BceM_CEP1 is classified as a new temperate phage with lytic activity. The findings of this study enhance our understanding of the diversity of Bcc phages.

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Bacteriophage therapy to combat MDR non-fermenting Gram-negative bacteria causing nosocomial infections: recent progress and challenges
Sunil Kumar, Razique Anwer, Anil Sharma, Mukesh Yadav, Nirmala Sehrawat
Naunyn-Schmiedeberg's Archives of Pharmacology.2025; 398(11): 15037. CrossRef

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NOTE] Diversity Analysis of Burkholderia cepacia Complex in the Water Bodies of West Lake, Hangzhou, China: Yuan Fang , Guan-lin Xie , Miao-miao Lou , Bin Li , Ibrahim Muhammad; J. Microbiol. 2011;49(2):309-314. Published online May 3, 2011; DOI: https://doi.org/10.1007/s12275-011-0267-2

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Abstract PDF

A survey of Burkholderia cepacia complex (Bcc) species was conducted in water bodies of West Lake in China. A total of 670 bacterial isolates were recovered on selective media. Out of them, 39.6% (265 isolates) were assigned to the following species: Burkholderia multivorans, Burkholderia cenocepacia recA lineage IIIA,
IIIB, Burkholderia stabilis, Burkholderia vietnamiensis, and Burkholderia seminalis while B. cenocepacia is documented as a dominant Bcc species in water of West Lake. In addition, all Bcc isolates tested were PCR negative for the cblA and esmR transmissibility marker genes except B. cenocepacia IIIB A8 which was positive for esmR genelater. The present study raises great concerns on the role of West Lake as a “reservoir” for potential Bcc pathogenic strains.

Citations

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Colony morphotype variation in Burkholderia: implications for success of applications and therapeutics
Pauline M. L. Coulon, Kirsty Agnoli, Garry S. A. Myers, Melissa M. Kendall
Journal of Bacteriology.2025;[Epub] CrossRef
Development of Droplet Digital PCR Assay for Detection of Seed-Borne Burkholderia glumae and B. gladioli Causing Bacterial Panicle Blight Disease of Rice
Jiannan Zhang, Jinyan Luo, Lei Chen, Temoor Ahmed, Saqer S. Alotaibi, Yanli Wang, Guochang Sun, Bin Li, Qianli An
Microorganisms.2022; 10(6): 1223. CrossRef
Opportunistic Pathogens of Recreational Waters with Emphasis on Antimicrobial Resistance—A Possible Subject of Human Health Concern
Joanna Stec, Urszula Kosikowska, Mariola Mendrycka, Dagmara Stępień-Pyśniak, Paulina Niedźwiedzka-Rystwej, Dominika Bębnowska, Rafał Hrynkiewicz, Joanna Ziętara-Wysocka, Ewelina Grywalska
International Journal of Environmental Research and Public Health.2022; 19(12): 7308. CrossRef
Burkholderia cepacia Complex Bacteria: a Feared Contamination Risk in Water-Based Pharmaceutical Products
Mariana Tavares, Mariya Kozak, Alexandra Balola, Isabel Sá-Correia
Clinical Microbiology Reviews.2020;[Epub] CrossRef
Environmental interactions are regulated by temperature in Burkholderia seminalis TC3.4.2R3
Priscila Jane Romano de Oliveira Gonçalves, Carmen C. Denman Hume, Almir José Ferreira, Sarina Tsui, Marcelo Brocchi, Brendan W. Wren, Welington Luiz Araujo
Scientific Reports.2019;[Epub] CrossRef
Diversity of culturable Gram-negative bacteria isolated from irrigation water of two rice crop regions in Southern Brazil
Maria Helena Lima Ribeiro Reche, Catiusca Reali, Michele Pittol, Danilo de Athayde Saul, Vera Regina Mussoi Macedo, Victor Hugo Valiati, Vilmar Machado, Lidia Mariana Fiuza
Environmental Monitoring and Assessment.2016;[Epub] CrossRef
Extensive cultivation of soil and water samples yields various pathogens in patients with cystic fibrosis but not Burkholderia multivorans
Charlotte Peeters, Eliza Depoorter, Jessy Praet, Peter Vandamme
Journal of Cystic Fibrosis.2016; 15(6): 769. CrossRef
PCR detection of Burkholderia multivorans in water and soil samples
Charlotte Peeters, Stijn Daenekindt, Peter Vandamme
BMC Microbiology.2016;[Epub] CrossRef
Spatial and temporal distributions and variations of nutrients in the West Lake, Hangzhou, after the implementation of integrated water management program (19852013)
YOU Aiju, WU Zhiying, HAN Zengcui, YANG Jun, HUA Lei
Journal of Lake Sciences.2015; 27(3): 371. CrossRef
Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde
Bin Li, Chang-Lin Shan, Qing Zhou, Yuan Fang, Yang-Li Wang, Fei Xu, Li-Rong Han, Muhammad Ibrahim, Long-Biao Guo, Guan-Lin Xie, Guo-Chang Sun
Marine Drugs.2013; 11(5): 1534. CrossRef
Diversity of potential pathogenicity and biofilm formation among Burkholderia cepacia complex water, clinical, and agricultural isolates in China
Muhammad Ibrahim, Qiaomei Tang, Yu Shi, Abdulwareth Almoneafy, Yuan Fang, Liuhu Xu, Wen Li, Bin Li, Guan-Lin Xie
World Journal of Microbiology and Biotechnology.2012; 28(5): 2113. CrossRef
Copper as an antibacterial agent for human pathogenic multidrug resistant Burkholderia cepacia complex bacteria
Muhammad Ibrahim, Fang Wang, Miao-miao Lou, Guan-lin Xie, Bin Li, Zhu Bo, Gou-qing Zhang, He Liu, Abdul Wareth
Journal of Bioscience and Bioengineering.2011; 112(6): 570. CrossRef

Journal Article

Burkholderia cepacia Complex Infection in a Cohort of Italian Patients with Cystic Fibrosis: Antonietta Lambiase , Valeria Raia , Stefania Stefani , Angela Sepe , Pasqualina Ferri , Paolo Buonpensiero , Fabio Rossano , Mariassunta Del Pezzo; J. Microbiol. 2007;45(3):275-279.; DOI: https://doi.org/2530 [pii]

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Abstract PDF: The aims of this study were to detect Burkholderia cepacia complex (Bcc) strains in a cohort of Cystic Fibrosis patients (n=276) and to characterize Bcc isolates by molecular techniques. The results showed that 11.23% of patients were infected by Bcc. Burkholderia cenocepacia lineage III-A was the most prevalent species (64.3%) and, of these, 10% was cblA positive and 50% esmR positive. Less than half of the strains were sensitive to ceftazidime, meropenem, piperacillin tazobactam, and trimethoprim-sulfamethoxazole. About half of the strains (41%) had homogeneous profiles, suggesting cross-transmission. The infection by B. cenocepacia was associated to a high rate of mortality (p=0.01).

Research Support, Non-U.S. Gov't

A Bacterium Belonging to the Burkholderia cepacia Complex Associated with Pleurotus ostreatus: Ricardo Yara , Walter Maccheroni Junior , Jorge Horii , Joao Lucio Azevedo; J. Microbiol. 2006;44(3):263-268.; DOI: https://doi.org/2387 [pii]

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Abstract PDF: Pleurotus ostreatus is a widely cultivated white-rot fungus. Owing to its considerable enzymatic versatility P. ostreatus has become the focus of increasing attention for its possible utility in biobleaching and bioremediation applications. Interactions between microorganisms can be an important factor in those processes. In this study, we describe the presence of a bacterial species associated with P. ostreatus strain G2. This bacterial species grew slowly (approximately 30 days) in theliquid and semi-solid media tested. When P. ostreatus was inoculated in solid media containing Tween 80 or Tween 20, bacterial microcolonies were detected proximal to the fungal colonies, and the relevant bacterium was identified via the analysis of a partial 16S rDNA sequence; it was determined to belong to the Burkholderia cepacia complex, but was not closely related to other fungus-isolated Burkholderiaceae. New specific primers were designed, and confirmed the presence of in vitro P. ostreatus cultures. This is the first time that a bacterial species belonging to the B. cepacia complex has been found associated with P. ostreatus.

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