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- Genetic diversity of Clavispora lusitaniae isolated from Agave fourcroydes Lem, as revealed by DNA fingerprinting
- Daisy Pérez-Brito , Anuar Magaña-Alvarez , Patricia Lappe-Oliveras , Alberto Cortes-Velazquez , Claudia Torres-Calzada , Teófilo Herrera-Suarez , Alfonso Larqué-Saavedra , Raul Tapia-Tussell
- J. Microbiol. 2015;53(1):14-20. Published online January 4, 2015
- DOI: https://doi.org/10.1007/s12275-015-4373-4
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Abstract
- This study characterized Clavispora lusitaniae strains isolated from different stages of the processing and early fermentation of a henequen (Agave fourcroydes) spirit produced in Yucatan, Mexico using a molecular technique. Sixteen strains identified based on morphological features, obtained from different substrates, were typed molecularly. Nine different versions of the divergent D1/D2 domain of the large-subunit ribosomal DNA sequence were identified among the C. lusitaniae strains. The greatest degree of polymorphism was found in the 90-bp structural motif of the D2 domain. The MSP-PCR technique was able to differentiate 100% of the isolates. This study provides significant insight into the genetic diversity of the mycobiota present during the henequen fermentation process, especially that of C. lusitaniae, for which only a few studies in plants have been published. The applied MSP-PCR markers were very efficient in revealing polymorphisms between isolates of this species.
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Citations
Citations to this article as recorded by
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- Clavispora lusitaniae: From a saprophytic yeast to an emergent pathogen
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