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- Effects of digested Cheonggukjang on human microbiota assessed by in vitro fecal fermentation
- Vineet Singh , Nakwon Hwang , Gwangpyo Ko , Unno Tatsuya
- J. Microbiol. 2021;59(2):217-227. Published online February 1, 2021
- DOI: https://doi.org/10.1007/s12275-021-0525-x
- 53 View
- 0 Download
- 13 Web of Science
- 13 Crossref
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Abstract
- In vitro fecal fermentation is an assay that uses fecal microbes to ferment foods, the results of which can be used to evaluate the potential of prebiotic candidates. To date, there have been various protocols used for in vitro fecal fermentation- based assessments of food substances. In this study, we investigated how personal gut microbiota differences and external factors affect the results of in vitro fecal fermentation assays. We used Cheonggukjang (CGJ), a Korean traditional fermented soybean soup that is acknowledged as healthy functional diet. CGJ was digested in vitro using acids and enzymes, and then fermented with human feces anaerobically. After fecal fermentation, the microbiota was analyzed using MiSeq, and the amount of short chain fatty acids (SCFAs) were measured using GC-MS. Our results suggest that CGJ was effectively metabolized by fecal bacteria to produce SCFAs, and this process resulted in an increase in the abundance of Coprococcus, Ruminococcus, and Bifidobacterium and a reduction in the growth of Sutterella, an opportunistic pathogen. The metabolic activities predicted from the microbiota shifts indicated enhanced metabolism linked to methionine biosynthesis and depleted chondroitin sulfate degradation. Moreover, the amount of SCFAs and microbiota shifts varied depending on personal microbiota differences. Our findings also suggest that in vitro fecal fermentation of CGJ for longer durations may partially affect certain fecal microbes. Overall, the study discusses the usability of in vitro gastrointestinal digestion and fecal fermentation (GIDFF) to imitate the effects of diet-induced microbiome modulation and its impact on the host.
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Citations
Citations to this article as recorded by
- Significance of Soy-Based Fermented Food and Their Bioactive Compounds Against Obesity, Diabetes, and Cardiovascular Diseases
Sushmita Dwivedi, Vineet Singh, Kritika Sharma, Amani Sliti, Mamta Baunthiyal, Jae-Ho Shin
Plant Foods for Human Nutrition.2024; 79(1): 1. CrossRef - Fermented foods: Harnessing their potential to modulate the microbiota-gut-brain axis for mental health
Ramya Balasubramanian, Elizabeth Schneider, Eoin Gunnigle, Paul D. Cotter, John F. Cryan
Neuroscience & Biobehavioral Reviews.2024; 158: 105562. CrossRef - The nutrition and therapeutic potential of millets: an updated narrative review
Jinu Jacob, Veda Krishnan, Chris Antony, Masimukka Bhavyasri, C. Aruna, Kiran Mishra, Thirunavukkarasu Nepolean, Chellapilla Tara Satyavathi, Kurella B. R. S. Visarada
Frontiers in Nutrition.2024;[Epub] CrossRef - Effects of OsomeFood Clean Label plant-based meals on the gut microbiome
Dwiyanto Jacky, Chia Bibi, Look Melvin Chee Meng, Fong Jason, Tan Gwendoline, Lim Jeremy, Chong Chun Wie
BMC Microbiology.2023;[Epub] CrossRef - Fermented soybean foods and diabetes
Yoshitaka Hashimoto, Masahide Hamaguchi, Michiaki Fukui
Journal of Diabetes Investigation.2023; 14(12): 1329. CrossRef - Early response of the gut microbiome and serum metabolites to Cheonggukjang intake in healthy Korean subjects
Eun-Ji Song, Min Jung Kim, Chang Hwa Jung, Won-Hyong Chung, Young-Do Nam, Mi Young Lim
Journal of Functional Foods.2023; 101: 105420. CrossRef - Role, relevance, and possibilities of in vitro fermentation models in human dietary, and gut‐microbial studies
Vineet Singh, HyunWoo Son, GyuDae Lee, Sunwoo Lee, Tatsuya Unno, Jae‐Ho Shin
Biotechnology and Bioengineering.2022; 119(11): 3044. CrossRef - Evaluation of Prebiotics through an In Vitro Gastrointestinal Digestion and Fecal Fermentation Experiment: Further Idea on the Implementation of Machine Learning Technique
Hokyung Song, Dabin Jeon, Tatsuya Unno
Foods.2022; 11(16): 2490. CrossRef - Anti-diabetic prospects of dietary bio-actives of millets and the significance of the gut microbiota: A case of finger millet
Vineet Singh, GyuDae Lee, HyunWoo Son, Sliti Amani, Mamta Baunthiyal, Jae-Ho Shin
Frontiers in Nutrition.2022;[Epub] CrossRef - Current Perspectives on the Physiological Activities of Fermented Soybean-Derived Cheonggukjang
Il-Sup Kim, Cher-Won Hwang, Woong-Suk Yang, Cheorl-Ho Kim
International Journal of Molecular Sciences.2021; 22(11): 5746. CrossRef - Alleviation of Neuronal Cell Death and Memory Deficit with Chungkookjang Made with Bacillus amyloliquefaciens and Bacillus subtilis Potentially through Promoting Gut–Brain Axis in Artery-Occluded Gerbils
Ting Zhang, Myeong-Seon Ryu, Xuangao Wu, Hee-Jong Yang, Su Ji Jeong, Ji-Won Seo, Do-Yeon Jeong, Sunmin Park
Foods.2021; 10(11): 2697. CrossRef - The final fate of food: On the establishment of in vitro colon models
Saartje Hernalsteens, Song Huang, Hai Hua Cong, Xiao Dong Chen
Food Research International.2021; 150: 110743. CrossRef - In Vitro Simulation of Human Colonic Fermentation: A Practical Approach towards Models’ Design and Analytical Tools
Elena Veintimilla-Gozalbo, Andrea Asensio-Grau, Joaquim Calvo-Lerma, Ana Heredia, Ana Andrés
Applied Sciences.2021; 11(17): 8135. CrossRef
- Significance of Soy-Based Fermented Food and Their Bioactive Compounds Against Obesity, Diabetes, and Cardiovascular Diseases
Review
- Functional interplay between the oxidative stress response and DNA damage checkpoint signaling for genome maintenance in aerobic organisms
- Ji Eun Choi , Woo-Hyun Chung
- J. Microbiol. 2020;58(2):81-91. Published online December 23, 2019
- DOI: https://doi.org/10.1007/s12275-020-9520-x
- 45 View
- 0 Download
- 10 Web of Science
- 9 Crossref
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Abstract
- The DNA damage checkpoint signaling pathway is a highly conserved surveillance mechanism that ensures genome integrity by sequential activation of protein kinase cascades. In mammals, the main pathway is orchestrated by two central sensor kinases, ATM and ATR, that are activated in response to DNA damage and DNA replication stress. Patients lacking functional ATM or ATR suffer from ataxia-telangiectasia (A-T) or Seckel syndrome, respectively, with pleiotropic degenerative phenotypes. In addition to DNA strand breaks, ATM and ATR also respond to oxidative DNA damage and reactive oxygen species (ROS), suggesting an unconventional function as regulators of intracellular redox status. Here, we summarize the multiple roles of ATM and ATR, and of their orthologs in Saccharomyces cerevisiae, Tel1 and Mec1, in DNA damage checkpoint signaling and the oxidative stress response, and discuss emerging ideas regarding the possible mechanisms underlying the elaborate crosstalk between those pathways. This review may provide new insights into the integrated cellular strategies responsible for maintaining genome stability in eukaryotes with a focus on the yeast model organism.
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Citations
Citations to this article as recorded by- DSB-induced oxidative stress: Uncovering crosstalk between DNA damage response and cellular metabolism
Xinyu Li, Caini Yang, Hengyu Wu, Hongran Chen, Xing Gao, Sa Zhou, Tong-Cun Zhang, Wenjian Ma
DNA Repair.2024; 141: 103730. CrossRef - Effects of Stress on Biological Characteristics and Metabolism of Periodontal Ligament Stem Cells of Deciduous Teeth
Zhengyang Li, Jinyi Li, Shanshan Dai, Xuelong Su, Meiyue Ren, Shuyang He, Qingyu Guo, Fei Liu
International Dental Journal.2024;[Epub] CrossRef - Signification and Application of Mutator and Antimutator Phenotype-Induced Genetic Variations in Evolutionary Adaptation and Cancer Therapeutics
Woo-Hyun Chung
Journal of Microbiology.2023; 61(12): 1013. CrossRef - Metabolic Stress and Mitochondrial Dysfunction in Ataxia-Telangiectasia
Goutham Narayanan Subramanian, Abrey Jie Yeo, Magtouf Hnaidi Gatei, David John Coman, Martin Francis Lavin
Antioxidants.2022; 11(4): 653. CrossRef - The Rad9–Rad1–Hus1 DNA Repair Clamp is Found in Microsporidia
Anne Caroline Mascarenhas dos Santos, Alexander Thomas Julian, Jean-François Pombert, Emmanuelle Lerat
Genome Biology and Evolution.2022;[Epub] CrossRef - Novel insights into the mechanism of cell cycle kinases Mec1(ATR) and Tel1(ATM)
Elias A. Tannous, Peter M. Burgers
Critical Reviews in Biochemistry and Molecular Biology.2021; 56(5): 441. CrossRef - DNA damage checkpoint and repair: From the budding yeast Saccharomyces cerevisiae to the pathogenic fungus Candida albicans
Shuangyan Yao, Yuting Feng, Yan Zhang, Jinrong Feng
Computational and Structural Biotechnology Journal.2021; 19: 6343. CrossRef - Acute Toxicity and DNA Instability Induced by Exposure to Low Doses of Triclosan and Phthalate DEHP, and Their Combinations, in vitro
Nathalia de Assis Aguilar Duarte, Lindiane Eloisa de Lima, Flora Troina Maraslis, Michael Kundi, Emilene Arusievicz Nunes, Gustavo Rafael Mazzaron Barcelos
Frontiers in Genetics.2021;[Epub] CrossRef - The mechanism and prevention of mitochondrial injury after exercise
Mingzhe Li, Baoan Ning, Tianhui Wang
Journal of Physiology and Biochemistry.2021; 77(2): 215. CrossRef
- DSB-induced oxidative stress: Uncovering crosstalk between DNA damage response and cellular metabolism
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