MSK
Search
- Page Path
- HOME > Search
Research Support, Non-U.S. Gov't
- NOTE] Molecular Analysis of the fur (ferric uptake regulator) Gene of a Pathogenic Edwardsiella tarda Strain
- Fang Wang , Shuang Cheng , Kun Sun , Li Sun
- J. Microbiol. 2008;46(3):350-355. Published online July 5, 2008
- DOI: https://doi.org/10.1007/s12275-008-0038-x
- 33 View
- 0 Download
- 32 Scopus
-
Abstract
- The gene encoding the Edwardsiella tarda ferric uptake regulator (FurEt) was cloned from a pathogenic E. tarda strain isolated from diseased fish. FurEt shares 90% overall sequence identity with the Escherichia coli Fur (FurEc) and was able to complement the mutant phenotype of a furEc-defective E. coli strain. Mutational analysis indicated that C92S and C95S mutations inactivated FurEt whereas E112K mutation resulted in a superactive FurEt variant. FurEt negatively regulated its own expression; interruption of this regulation impaired bacterial growth, altered the production of certain outer membrane proteins, and attenuated bacterial virulence.
First
Prev
TOP
