Journal of Microbiology

Journal Articles

The Salmonella enterica EnvE is an Outer Membrane Lipoprotein and Its Gene Expression Leads to Transcriptional Repression of the Virulence Gene msgA: Sinyeon Kim, Yong Heon Lee; J. Microbiol. 2024;62(11):1013-1022. Published online November 15, 2024; DOI: https://doi.org/10.1007/s12275-024-00183-4

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Abstract: The envE gene of Salmonella enterica serovar Typhimurium is encoded within Salmonella Pathogenicity Island-11 (SPI-11) and is located immediately upstream of the virulence gene msgA (macrophage survival gene A) in the same transcriptional orientation. To date, the characteristics and roles of envE remain largely unexplored. In this study, we show that EnvE, a predicted lipoprotein, is localized on the outer membrane using sucrose gradient ultracentrifugation. Under oxidative stress conditions, envE transcription is suppressed, while msgA transcription is induced, indicating an inverse correlation between the mRNA levels of the two neighboring genes. Importantly, inactivation of envE leads to constitutive transcription of msgA regardless of the presence of oxidative stress. Moreover, trans-complementation of the envE mutant with a plasmid-borne envE fails to prevent the induction of msgA transcription, suggesting that envE functions as a cis-regulatory element rather than a trans-acting factor. We further show that both inactivation and complementation of envE confer wild-type levels of resistance to oxidative stress by ensuring the expression of msgA. Our data suggest that the S. enterica envE gene encodes an outer membrane lipoprotein, and its transcription represses msgA expression in a cis-acting manner, probably by transcriptional interference, although the exact molecular details are yet unclear.

Anti-inflammatory and anti-oxidative effect of Korean propolis on Helicobacter pylori-induced gastric damage in vitro: Moon-Young Song , Da-Young Lee , Eun-Hee Kim; J. Microbiol. 2020;58(10):878-885. Published online September 2, 2020; DOI: https://doi.org/10.1007/s12275-020-0277-z

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Helicobacter pylori, present in the stomach lining, is a Gramnegative bacterium that causes various gastrointestinal diseases, including gastritis and peptic ulcers. Propolis is a natural resinous substance collected from a variety of plants, and contains several natural bioactive substances. The aim of this study was to investigate the anti-inflammatory and antioxidative effects of Korean propolis on H. pylori-induced damage in the human adenocarcinoma gastric cell line. The propolis used in this study was obtained from the Korea Beekeeping Association in South Korea. The expression of pro-inflammatory interleukins (ILs), such as IL-8, IL-12, IL-1β, tumor necrosis factor alpha, cyclooxygenase-2, and inducible nitric oxide synthase, which was increased after H. pylori infection, significantly decreased in a dose-dependent manner upon pretreatment with Korean propolis, because of the suppression of mitogen-activated protein kinases and nuclear factor κB pathway. The anti-oxidative activity of propolis was assessed using the 2,2-diphenyl-1-picrylhydrazyl hydrate free radical assay. Korean propolis showed significant anti-oxidative effects via reactive oxygen species scavenging. In addition, pretreatment with Korean propolis upregulated the expression of anti-oxidant enzymes through Nrf2 signaling activation. These findings indicate that the use of Korean propolis, which has anti-inflammatory and anti-oxidative effects, can be promising for the prevention of H. pylori-induced gastric damage.

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Network Pharmacology and Experimental Validation Reveal Therapeutic Potential of Propolis in UV-Induced Allergic Dermatitis
Liyuan Cheng, Jie Wang, Yicong Wang, Jingjing Li, Wenchao Yang
Foods.2025; 14(6): 996. CrossRef
Propolis as an autophagy modulator in relation to its roles in redox balance and inflammation regulation
R. Lesmana, S. Tandean, A. Christoper, A.A. Suwantika, N. Wathoni, R. Abdulah, J. Fearnley, V. Bankova, F. Zulhendri
Biomedicine & Pharmacotherapy.2024; 175: 116745. CrossRef
Exploring the Prospective Role of Propolis in Modifying Aging Hallmarks
Carla Scorza, Valeria Goncalves, Josef Finsterer, Fúlvio Scorza, Fernando Fonseca
Cells.2024; 13(5): 390. CrossRef
Development of a chitosan/propolis-based polymeric system: Characterization, biocompatibility, and modulation of transcription factor expression
Raquel Velázquez-Rodríguez, Amaury Pozos-Guillén, Martha Gabriela Chuc-Gamboa, Juan Valerio Cauich-Rodríguez, Héctor Flores-Reyes, Francisco Javier Tejeda-Nava, Fernando Javier Aguilar-Perez, Diana Maria Escobar Garcia
Journal of Bioactive and Compatible Polymers.2024; 39(5): 329. CrossRef
Therapeutic potential of propolis in alleviating inflammatory response and promoting wound healing in skin burn
Christian Oktavianus Manginstar, Trina Ekawati Tallei, Nurdjannah Jane Niode, Christina Leta Salaki, Sofia Safitri Hessel
Phytotherapy Research.2024; 38(2): 856. CrossRef
A review for non-antibiotic treatment of Helicobacter pylori: new insight
Neda Shadvar, Sousan Akrami, Seyyed-Mohammad-Amin Mousavi Sagharchi, Rafee Habib Askandar, Alireza Merati, Masoomeh Aghayari, Nikki Kaviani, Hamed Afkhami, Mojtaba Kashfi
Frontiers in Microbiology.2024;[Epub] CrossRef
Wound Healing, Anti-Inflammatory and Anti-Oxidant Activities, and Chemical Composition of Korean Propolis from Different Sources
Aman Dekebo, Chalshisa Geba, Daniel Bisrat, Jin Boo Jeong, Chuleui Jung
International Journal of Molecular Sciences.2024; 25(21): 11352. CrossRef
Effects of oxidative stress regulation in inflammation-associated gastric cancer progression treated using traditional Chinese medicines: A review
Bo Chen, Xinqian Dong, Jinlong Zhang, Wei Wang, Yujiao Song, Xitong Sun, Kangning Zhao, Zhen Sun
Medicine.2023; 102(46): e36157. CrossRef
Emodin Attenuates Inflammasome Activation Induced by Helicobacter pylori Infection through Inhibition of VacA Translocation
Thach Phan Van, Anh Duy Do
Microbiology and Biotechnology Letters.2023; 51(4): 507. CrossRef
Correlation between Chemical Profile of Georgian Propolis Extracts and Their Activity against Helicobacter pylori
Jarosław Widelski, Piotr Okińczyc, Katarzyna Suśniak, Anna Malm, Anna Bozhadze, Malkhaz Jokhadze, Izabela Korona-Głowniak
Molecules.2023; 28(3): 1374. CrossRef
Therapeutic effect of propolis nanoparticles on wound healing
Juan Yang, Yingjuan He, Sha Nan, Juan Li, Anjuan Pi, Lele Yan, Jinshan Xu, Yuhui Hao
Journal of Drug Delivery Science and Technology.2023; 82: 104284. CrossRef
Recent Update on the Anti-Inflammatory Activities of Propolis
Felix Zulhendri, Ronny Lesmana, Steven Tandean, Andreas Christoper, Kavita Chandrasekaran, Ilham Irsyam, Auliya A. Suwantika, Rizky Abdulah, Nasrul Wathoni
Molecules.2022; 27(23): 8473. CrossRef
Construction of a Cuprotosis-Related Gene-Based Model to Improve the Prognostic Evaluation of Patients with Gastric Cancer
Chunyan Han, Kai Zhang, XinKai Mo, Fu Wang
Journal of Immunology Research.2022; 2022: 1. CrossRef
Honey Bee Products: Preclinical and Clinical Studies of Their Anti-inflammatory and Immunomodulatory Properties
Hesham R. El-Seedi, Nehal Eid, Aida A. Abd El-Wahed, Mostafa E. Rateb, Hanan S. Afifi, Ahmed F. Algethami, Chao Zhao, Yahya Al Naggar, Sultan M. Alsharif, Haroon Elrasheid Tahir, Baojun Xu, Kai Wang, Shaden A. M. Khalifa
Frontiers in Nutrition.2022;[Epub] CrossRef
Plant-Based Polyphenols: Anti-Helicobacter pylori Effect and Improvement of Gut Microbiota
María Guerra-Valle, Patricio Orellana-Palma, Guillermo Petzold
Antioxidants.2022; 11(1): 109. CrossRef
DETERMINATION OF ANTI-UREASE ACTIVITY OF PROPOLIS FROM MARMARA REGION OF TURKEY
Zehra Can, Yakup Kara, Sevgi Kolaylı, İbrahim Çakmak
Uludağ Arıcılık Dergisi.2022; 22(1): 25. CrossRef
Nrf2 Downregulation Contributes to Epithelial-to-Mesenchymal Transition in Helicobacter pylori-Infected Cells
Sarah Bacon, Lornella Seeneevassen, Alison Fratacci, Faustine Rose, Camille Tiffon, Elodie Sifré, Maria M. Haykal, Maya M. Moubarak, Astrid Ducournau, Lucie Bruhl, Stéphane Claverol, Caroline Tokarski, Alina-Roxani Gouloumi, Ioannis S. Pateras, Thomas Dau
Cancers.2022; 14(17): 4316. CrossRef
Anti-Inflammatory Effect of Korean Propolis on Helicobacter pylori-Infected Gastric Mucosal Injury Mice Model
Moon-Young Song, Da-Young Lee, Young-Min Han, Eun-Hee Kim
Nutrients.2022; 14(21): 4644. CrossRef
Antifungal Properties of Chemically Defined Propolis from Various Geographical Regions
Marcin Ożarowski, Tomasz M. Karpiński, Rahat Alam, Małgorzata Łochyńska
Microorganisms.2022; 10(2): 364. CrossRef
The Antimicrobial Properties of Poplar and Aspen–Poplar Propolises and Their Active Components against Selected Microorganisms, including Helicobacter pylori
Jarosław Widelski, Piotr Okińczyc, Emil Paluch, Tomasz Mroczek, Jakub Szperlik, Magdalena Żuk, Zbigniew Sroka, Zuriyadda Sakipova, Ioanna Chinou, Krystyna Skalicka-Woźniak, Anna Malm, Izabela Korona-Głowniak
Pathogens.2022; 11(2): 191. CrossRef
Research Progress on Therapeutic Effect and Mechanism of Propolis on Wound Healing
Juan Yang, Anjuan Pi, Lele Yan, Juan Li, Sha Nan, Jing Zhang, Yuhui Hao, Juraj Majtan
Evidence-Based Complementary and Alternative Medicine.2022; 2022: 1. CrossRef
"GREEN SYNTHESIS OF NANO-SILVER/SODIUM ALGINATE/CARBOXYMETHYL XANTHAN GUM HYDROGEL AND EVALUATION OF ITS ANTI-INFLAMMATORY AND ANTI-Helicobacter pylori ACTIVITY "
NOURA Y. ELMEHBAD, NADIA A. MOHAMED, NAHED A. ABD EL-GHANY, MARWA M. ABDEL-AZIZ
Cellulose Chemistry and Technology.2022; 56(9-10): 983. CrossRef
Propolis-Loaded Chitosan Nanoparticles for the Treatment of Bacterial Diarrhea: In Vitro and In Vivo Evaluation
Jie Qiao, Jun Yang, Saman Jalili
Journal of Biomedical Nanotechnology.2022; 18(7): 1826. CrossRef
Steamed Ginger Extract Exerts Anti-inflammatory Effects in Helicobacter pylori-infected Gastric Epithelial Cells through Inhibition of NF-κB
Moon-Young Song, Da-Young Lee, Sang-Yong Park, Seul-A Seo, Jeong-Seung Hwang, Soo-Hyeon Heo, Eun-Hee Kim
Journal of Cancer Prevention.2021; 26(4): 289. CrossRef
Short‐term efficacy of a gel containing propolis extract, nanovitamin C and nanovitamin E on peri‐implant mucositis: A double‐blind, randomized, clinical trial
José González‐Serrano, Rosa María López‐Pintor, Julia Serrano, Jesús Torres, Gonzalo Hernández, Mariano Sanz
Journal of Periodontal Research.2021; 56(5): 897. CrossRef
Experimental Evidence for Therapeutic Potentials of Propolis
Priyanshu Bhargava, Debajit Mahanta, Ashish Kaul, Yoshiyuki Ishida, Keiji Terao, Renu Wadhwa, Sunil C. Kaul
Nutrients.2021; 13(8): 2528. CrossRef
Propolis and Its Gastroprotective Effects on NSAID-Induced Gastric Ulcer Disease: A Systematic Review
Porfirio Alonso Ruiz-Hurtado, Leticia Garduño-Siciliano, Pilar Domínguez-Verano, Daniela Balderas-Cordero, Gustavo Gorgua-Jiménez, Octavio Canales-Álvarez, María Margarita Canales-Martínez, Marco Aurelio Rodríguez-Monroy
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Molecular Insights into the Antistress Potentials of Brazilian Green Propolis Extract and Its Constituent Artepillin C
Ashish Kaul, Raviprasad Kuthethur, Yoshiyuki Ishida, Keiji Terao, Renu Wadhwa, Sunil C. Kaul
Molecules.2021; 27(1): 80. CrossRef

Impact of feeding regimens on the composition of gut microbiota and metabolite profiles of plasma and feces from Mongolian sheep: Bohui Wang , Yulong Luo , Rina Su , Duo Yao , Yanru Hou , Chang Liu , Rui Du , Ye Jin; J. Microbiol. 2020;58(6):472-482. Published online April 22, 2020; DOI: https://doi.org/10.1007/s12275-020-9501-0

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Abstract

Mongolian sheep are an indigenous ruminant raised for wool and meat production in China. The gut microbial community plays an important role in animal performance and metabolism. The objective of this study was to investigate the effects of two feeding regimens on the diversity and composition of gut microbiota and metabolite profiles of feces and plasma from Mongolian sheep. A total of 20 Mongolian sheep were assigned to one of two feeding regimens: free grazing (FG) and barn confinement (BC). When samples were collected, the average live weights of the sheep were 31.28 ± 1.56 kg and 34.18 ± 1.87 kg for the FG and BC groups, respectively. At the genus level, the FG group showed higher levels of Bacteroides, RC9_gut_group, Alistipes, Phocaeicola, Barnesiella, and Oscillibacter, and lower levels of Succinivibrio, Treponema, and Prevotella, compared to the BC group. The butyric acid content in feces was lower in the FG group (P < 0.05). Higher levels of palmitic acid, oleic acid, alpha-linolenic acid, L-carnitine, L-citrulline, and L-histidine, and lower levels of L-tyrosine, L-phenylalanine, and L-kynurenine were found in the plasma of the FG sheep. Moreover, there were substantial associations between several gut microbiota genera and alterations in feces and plasma metabolites especially those involved in the metabolism of butyric acid, linolenic acid, and L-tyrosine. Feeding regimens can not only influence the composition of gut microbiota, but also alter metabolic homeostasis in sheep.

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Research Support, Non-U.S. Gov't

NOTE] Envelope Diversity, Characteristics of V3 Region and Predicted Co-Receptor Usage of Human Immunodeficiency Viruses Infecting North Indians: Raiees Andrabi , Rajesh Kumar , Manju Bala , Ambili Nair , Prakash SS , Vandana Kushwaha , Kalpana Luthra; J. Microbiol. 2012;50(5):869-873. Published online November 4, 2012; DOI: https://doi.org/10.1007/s12275-012-2136-z

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Abstract: Subtypes of human immunodeficiency virus type 1 circulating in 21 north Indian patients were characterized based on the partial sequence of the gp120 envelope protein. A majority of viruses (85.7%, 18/21) were subtype C, while 14.3% (3/21) were subtype A. Sequence analysis revealed that the V3 region was highly conserved compared with V4 and V5. The predicted use of co-receptors indicated exclusive usage of R5, except for two subtype A viruses (AIIMS279 and AIIMS281). Our results demonstrate conservation within the V3 loop of subtype C viruses, and suggest the emergence of non-clade C viruses in the north Indian population.

Production and Characterization of Monoclonal Antibodies to Human Immunodeficiency Virus Type 1 gp120 Envelope Glycoprotein: Choi, Eui Yul , Ryu, Ji Yoon , Lee, Yoon , Ha, Sung Gil , Chung, So Young , Park, Sang Yeol , Nham, Sang Uk , Lee, Young Ik , Park, Jin Seu; J. Microbiol. 1998;36(1):59-65.

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Abstract: Monoclonal antibodies to human immunodeficiency virus type 1 envelope glycoprotein gp 120(HIV-1 gp120) were produced and characterized. For immunogen recombinant gp120 polypeptide expressed in bacteria was prepared and injected into mice. From two fusion experiments, twenty hybridomas secreting monoclonal antibodies against the recombinant gp120 were initially screened by immunodot blot analysis. Among the antibodies, 15 of them showed strong reactivities with the recombinant protein expressed in bacteria in Western blot and thus it was tested if these could react with the recombinant protein expressed in insect cells. All of the 15 antibodies immunostained the protein band with varing degrees of reactivities. Next, we tested whether the antibodies recognize authentic gp120 protein expressed in mammalian cells. COS-1 cells were tranfected with the cDNA encoding gp120 protein, and the transiently ecpressed protein were analyzed with the mAbs by Western blot analysis and immunofluorescence microscopy. Six of the monoclonal antibodies reacted with the protein band of authentic gp120 expressed in mammalian cells in the Western blot, and five stained the cell periphery of the transfected COS-1 cells in immunofluorescence. The mAbs described in this study should prove to be useful tools for the biochemical, immunological and structural analysis of HIV-1 gp120 envelope glycoprotein.

Characterization of an Attenuated Japanese Encephalitis Virus Adapted to African Green Nomkey Kidney Cells, Vero: Chung, Yong Ju , Hong, Sun Pyo , Moon, Sang Beom , Shin, Young Cheol , Kim, Soo Ok; J. Microbiol. 1998;36(3):189-195.

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Abstract: Live attenuated Japanese encephalitis (JE) virus SA14-14-2 produced in primary dog kidney cells (PDK) was adapted to African green monkey kidney cells, Vero. In an effort to gain insight into the molecular basis of the biological characteristics of the isolated SA14-14-2 (Vero) strain, the 1,500 nucleotide sequence encoding the envelope (E) gene which possesses major neutralizing epitopes was determined and dompared with the sequences of two other attenuated JE virus strains, SA14-14-2 (PHK) and SA14-14-2 (PDK). The amino acid sequence of the C-terminal region (a.a. 280-500) of the SA14-14-2 (Vero) E gene was found to be identical to those of strains SA14-14-2 (PHK) and SA14-14-2 (PDK), while the N-terminal region (a.a. 1-279) showed sequence variation. The distribution of mutations in the N-terminal region was nearly the same among the three attenuated strains, suggesting that the N-terminal sequences might be related with virus-host cell specificity. However, it was found that Lys and Val (a.a.138 and 176, respectively), known to be responsible for attenuation, are still conserved in SA14-14-2 (Vero). Animal testing showed that SA14-14-2 (Vero) has a neurovirulence phenotype similar to that of the parent SA14-14-2 (PDK) strain in suckling mice. The SA14-14-2 (Vero) grew very efficiently in Vero cells enough to support vaccine production. The growth characteristics of SA14-14-2 (Vero) in Vero cell and conservation of attenuation determinant of neurovirulence support that SA14-14-2 (Vero) could be developed as a new vaccine strain for human use.

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