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- Fluorescence change of Fusobacterium nucleatum due to Porphyromonas gingivalis
- Min-Ah Lee , Si-Mook Kang , Se-Yeon Kim , Ji-Soo Kim , Jin-Bom Kim , Seung-Hwa Jeong
- J. Microbiol. 2018;56(9):628-633. Published online August 23, 2018
- DOI: https://doi.org/10.1007/s12275-018-7515-7
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Abstract
- The aim of this study was to measure changes in the fluorescence of Fusobacterium nucleatum interacting with Porphyromonas gingivalis for excitation with blue light at 405-nm. P. gingivalis was mono- and co-cultivated in close proximity with F. nucleatum. The fluorescence of the bacterial colonies was photographed using a QLF-D (Quantitative Light-induced Fluorescence-Digital) Biluminator camera system with a 405 nm light source and a specific filter. The red, green and blue intensities of fluorescence images were analyzed using the image analysis software. A fluorescence spectrometer was used to detect porphyrin synthesized by each bacterium. F. nucleatum, which emitted green fluorescence in single cultures, showed intense red fluorescence when it was grown in close proximity with P. gingivalis. F. nucleatum co-cultivated with P. gingivalis showed the same pattern of fluorescence peaks as for protoporphyrin IX in the red part of the spectrum. We conclude that the green fluorescence of F. nucleatum can change to red fluorescence in the presence of adjacent co-cultured with P. gingivalis, indicating that the fluorescence character of each bacterium might depend on the presence of other bacteria.
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- Red/Orange Autofluorescence in Selected Candida Strains Exposed to 405 nm Laser Light
Rafał Wiench, Dariusz Paliga, Anna Mertas, Elżbieta Bobela, Anna Kuśka-Kiełbratowska, Sonia Bordin-Aykroyd, Aleksandra Kawczyk-Krupka, Kinga Grzech-Leśniak, Monika Lukomska-Szymanska, Edward Lynch, Dariusz Skaba
Dentistry Journal.2024; 12(3): 48. CrossRef - Autofluorescence Detection Method for Dental Plaque Bacteria Detection and Classification: Example of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Streptococcus mutans
Yung-Jhe Yan, Bo-Wen Wang, Chih-Man Yang, Ching-Yi Wu, Mang Ou-Yang
Dentistry Journal.2021; 9(7): 74. CrossRef - Fluorescence image and microbiological analysis of biofilm retained around healthy and inflamed orthodontic miniscrews
A.S. Garcez, L.C. Barros, M.R.U. Fernandes, D.N. Fujii, S.S. Suzuki, R. Nepomuceno
Photodiagnosis and Photodynamic Therapy.2020; 30: 101707. CrossRef
- Red/Orange Autofluorescence in Selected Candida Strains Exposed to 405 nm Laser Light
Research Support, Non-U.S. Gov't
- Development of Strain-specific PCR Primers Based on a DNA Probe Fu12 for the Identification of Fusobacterium nucleatum subsp. nucleatum ATCC 25586^T
- Hwa-Sook Kim , Soo Keun Song , So Young Yoo , Dong Chun Jin , Hwan Seon Shin , Chae Kwang Lim , Myung-Soo Kim , Jin-Soo Kim , Son-Jin Choe , Joong-Ki Kook
- J. Microbiol. 2005;43(4):331-336.
- DOI: https://doi.org/2257 [pii]
- 49 View
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Abstract
- The objective of this study was to assess the strain-specificity of a DNA probe, Fu12, for Fusobacterium nucleatum subsp. nucleatum ATCC 25586^T (F. nucleatum ATCC 25586^T), and to develop sets of strain-specific polymerase chain reaction (PCR) primers. Strain-specificity was tested against 16 strains of F. nucleatum and 3 strains of distinct Fusobacterium species. Southern blot hybridization revealed that the Fu12 reacted exclusively with the HindIII-digested genomic DNA of F. nucleatum ATCC 25586^T. The results of PCR revealed that three pairs of PCR primers, based on the nucleotide sequence of Fu12, generated the strain-specific amplicons from F. nucleatum ATCC 25586^T. These results suggest that the DNA probe Fu12 and the three pairs of PCR primers could be useful in the identification of F. nucleatum ATCC 25586^T, especially with regard to the determination of the authenticity of the strain.
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