Journal of Microbiology

Full article

Role of the LAMMER kinase LkhA in fungal development and aflatoxin production in Aspergillus flavus: Seong-Hwan Jeong, He-Jin Cho, Jae-Hyuk Yu, Hee-Moon Park, Hee-Soo Park; J. Microbiol. 2025;63(5):e2503007. Published online May 27, 2025; DOI: https://doi.org/10.71150/jm.2503007

166 View
8 Download

Abstract PDF: A well-conserved LAMMER kinase in yeast and filamentous fungi, is a dual-specificity kinase with multiple roles in fungal biology. In this study, we assessed the roles of LkhA in Aspergillus flavus, a toxigenic fungus that produces aflatoxin B1. lkhA deletion mutants exhibited defects in fungal growth, conidiophore development, and sclerotia formation. These mutants exhibited impaired tolerance to oxidative and cell wall stresses. Moreover, the absence of lkhA resulted in a decrease in aflatoxin B1 production. The kernel assay revealed that the lkhA deletion mutants exhibited reduced production of conidia and aflatoxin B1, implying that LkhA can affect fungal toxigenesis and pathogenicity. Taken together, these results demonstrate that LkhA is important for differentiation, mycotoxin production, and pathogenicity in A. flavus.

Journal Articles

LAMMER Kinase Governs the Expression and Cellular Localization of Gas2, a Key Regulator of Flocculation in Schizosaccharomyces pombe: Won-Hwa Kang , Yoon-Dong Park , Joo-Yeon Lim , Hee-Moon Park; J. Microbiol. 2024;62(1):21-31. Published online January 5, 2024; DOI: https://doi.org/10.1007/s12275-023-00097-7

76 View
0 Download

Abstract: It was reported that LAMMER kinase in Schizosaccharomyces pombe plays an important role in cation-dependent and galactose-specific flocculation. Analogous to other flocculating yeasts, when cell wall extracts of the Δlkh1 strain were treated to the wild-type strain, it displayed flocculation. Gas2, a 1,3-β-glucanosyl transferase, was isolated from the EDTA-extracted cell-surface proteins in the Δlkh1 strain. While disruption of the gas2+ gene was not lethal and reduced the flocculation activity of the Δlkh1 strain, the expression of a secreted form of Gas2, in which the GPI anchor addition sequences had been removed, conferred the ability to flocculate upon the WT strain. The Gas2-mediated flocculation was strongly inhibited by galactose but not by glucose. Immunostaining analysis showed that the cell surface localization of Gas2 was crucial for the flocculation of fission yeast. In addition, we identified the regulation of mbx2+ expression by Lkh1 using RT-qPCR. Taken together, we found that Lkh1 induces asexual flocculation by regulating not only the localization of Gas2 but also the transcription of gas2+ through Mbx2.

The putative C2H2 transcription factor RocA is a novel regulator of development and secondary metabolism in Aspergillus nidulans: Dong Chan Won , Yong Jin Kim , Da Hye Kim , Hee-Moon Park , Pil Jae Maeng; J. Microbiol. 2020;58(7):574-587. Published online April 22, 2020; DOI: https://doi.org/10.1007/s12275-020-0083-7

70 View
0 Download
3 Web of Science
2 Crossref

Abstract

Multiple transcriptional regulators play important roles in the coordination of developmental processes, including asexual and sexual development, and secondary metabolism in the filamentous fungus Aspergillus nidulans. In the present study, we characterized a novel putative C2H2-type transcription factor (TF), RocA, in relation to development and secondary metabolism. Deletion of rocA increased conidiation and caused defective sexual development. In contrast, the overexpression of rocA exerted opposite effects on both phenotypes. Additionally, nullifying rocA resulted in enhanced brlA expression and reduced nsdC expression, whereas its overexpression exerted the opposite effects. These results suggest that RocA functions as a negative regulator of asexual development by repressing the expression of brlA encoding a key asexual development activator, but as a positive regulator of sexual development by enhancing the expression of nsdC encoding a pivotal sexual development activator. Deletion of rocA increased the production of sterigmatocystin (ST), as well as the expression of its biosynthetic genes, aflR and stcU. Additionally, the expression of the biosynthetic genes for penicillin (PN), ipnA and acvA, and for terrequinone (TQ), tdiB and tdiE, was increased by rocA deletion. Thus, it appears that RocA functions as a negative transcriptional modulator of the secondary metabolic genes involved in ST, PN, and TQ biosynthesis. Taken together, we propose that RocA is a novel transcriptional regulator that may act either positively or negatively at multiple target genes necessary for asexual and sexual development and secondary metabolism.

Citations

Citations to this article as recorded by

srdA mutations suppress the rseA/cpsA deletion mutant conidiation defect in Aspergillus nidulans
Masahiro Ogawa, Ryouichi Fukuda, Ryo Iwama, Yasuji Koyama, Hiroyuki Horiuchi
Scientific Reports.2023;[Epub] CrossRef
Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in Chaetomium globosum
Shanshan Zhao, Kai Zhang, Congyu Lin, Ming Cheng, Jinzhu Song, Xin Ru, Zhengran Wang, Wan Wang, Qian Yang
International Journal of Molecular Sciences.2022; 23(23): 14849. CrossRef

Expression of sexual genes in Aspergillus fumigatus homogeneous culture produced by vegetative mass mating: Joo-Yeon Lim , Hee-Moon Park; J. Microbiol. 2019;57(8):688-693. Published online May 11, 2019; DOI: https://doi.org/10.1007/s12275-019-9094-7

74 View
0 Download
3 Web of Science
3 Crossref

Abstract

There are presently no studies on the genes for sexual development of Aspergillus fumigatus in situ using mating culture, primarily because of challenging experimental conditions that require a significantly long period of induction and produce developmentally heterogenous culture, harboring very few sexual organs. In order to overcome these challenges, we developed an efficient and convenient procedure called ‘vegetative mass mating (VeM)’ for study at a molecular level. The VeM method enabled production of a developmentally homogenous A. fumigatus culture, harboring many sexual organs in a plate within a short period of two weeks. Feasibility of the use of VeM for functional study of genes during A. fumigatus sexual development was evaluated by analyzing the transcription pattern of genes involved in pheromone signal transduction and regulation of sexual development. Here, we present for the first time, an in situ expression pattern of sexual genes during the mating process, induced by the VeM
method
, which will enable and promote the sexual development study of A. fumigatus at the molecular level.

Citations

Citations to this article as recorded by

The Gβ-like Protein AfCpcB Affects Sexual Development, Response to Oxidative Stress and Phagocytosis by Alveolar Macrophages in Aspergillus fumigatus
Joo-Yeon Lim, Yeon-Ju Kim, Hee-Moon Park
Journal of Fungi.2022; 8(1): 56. CrossRef
The LAMMER Kinase, LkhA, Affects Aspergillus fumigatus Pathogenicity by Modulating Reproduction and Biosynthesis of Cell Wall PAMPs
Joo-Yeon Lim, Yeon Ju Kim, Seul Ah Woo, Jae Wan Jeong, Yu-Ri Lee, Cheol-Hee Kim, Hee-Moon Park
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Global Sexual Fertility in the Opportunistic Pathogen Aspergillus fumigatus and Identification of New Supermater Strains
Sameira S. Swilaiman, Céline M. O’Gorman, Wenyue Du, Janyce A. Sugui, Joanne Del Buono, Matthias Brock, Kyung J. Kwon-Chung, George Szakacs, Paul S. Dyer
Journal of Fungi.2020; 6(4): 258. CrossRef

Research Support, Non-U.S. Gov'ts

Transcriptional Regulation of fksA, a β-1,3-Glucan Synthase Gene, by the APSES Protein StuA during Aspergillus nidulans Development: Bum-Chan Park , Yun-Hee Park , Soohyun Yi , Yu Kyung Choi , Eun-Hye Kang , Hee-Moon Park; J. Microbiol. 2014;52(11):940-947. Published online October 31, 2014; DOI: https://doi.org/10.1007/s12275-014-4517-y

71 View
0 Download
12 Crossref

Abstract

The temporal and spatial regulation of β-1,3-glucan synthesis plays an important role in morphogenesis during fungal growth and development. Northern blot analysis showed that the transcription of fksA, the gene encoding β-1,3-glucan synthase in Aspergillus nidulans, was cell-cycle-dependent and increased steadily over the duration of the vegetative period, but its overall expression during the asexual and sexual stages was fairly constant up until the time of transcription cessation. In an A. nidulans strain mutated in the eukaryotic bHLH-like APSES transcription factor stuA1, the transcriptional level of fksA, and consequently the content of alkali-insoluble cell wall β-glucan, significantly increased at the conidial chain formation and maturation stage. Electrophoretic mobility shift assays revealed that StuA was bound to StREs (StuA Response Elements) on the fksA promoter region. Promoter analysis with sGFP-fusion constructs also indicated the negative regulation of fksA expression by StuA, especially during asexual development. Taken together, these data suggest that StuA plays an important role in cell wall biogenesis during the development of A. nidulans, by controlling the transcription level of fksA.

Citations

Citations to this article as recorded by

Survival Factor A (SvfA) Contributes to Aspergillus nidulans Pathogenicity
Joo-Yeon Lim, Ye-Eun Jung, Hye-Eun Hwang, Cheol-Hee Kim, Nese Basaran-Akgul, Sri Harshini Goli, Steven P. Templeton, Hee-Moon Park
Journal of Fungi.2023; 9(2): 143. CrossRef
Potential utility of endophytic Bacillus altitudinis strain P32-3 as a biocontrol agent for the postharvest prevention of sweet potato black rot
Yong-Jing Zhang, Xiao-Ying Cao, Yu-Jie Chen, Hao Cong, Yi-Ming Wang, Ji-Hong Jiang, Lu-Dan Li
Biological Control.2023; 186: 105350. CrossRef
Survival factor SvfA plays multiple roles in differentiation and is essential for completion of sexual development in Aspergillus nidulans
Joo-Yeon Lim, Eun-Hye Kang, Yun-Hee Park, Jun-Ho Kook, Hee-Moon Park
Scientific Reports.2020;[Epub] CrossRef
Expression Analysis of Cell Wall-Related Genes in the Plant Pathogenic Fungus Drechslera teres
Aurélie Backes, Jean-Francois Hausman, Jenny Renaut, Essaid Ait Barka, Cédric Jacquard, Gea Guerriero
Genes.2020; 11(3): 300. CrossRef
Dynamic Transcriptomic and Phosphoproteomic Analysis During Cell Wall Stress in Aspergillus nidulans
Cynthia Chelius, Walker Huso, Samantha Reese, Alexander Doan, Stephen Lincoln, Kelsi Lawson, Bao Tran, Raj Purohit, Trevor Glaros, Ranjan Srivastava, Steven D. Harris, Mark R. Marten
Molecular & Cellular Proteomics.2020; 19(8): 1310. CrossRef
Molecular Dialogues between Early Divergent Fungi and Bacteria in an Antagonism versus a Mutualism
Olga A. Lastovetsky, Lev D. Krasnovsky, Xiaotian Qin, Maria L. Gaspar, Andrii P. Gryganskyi, Marcel Huntemann, Alicia Clum, Manoj Pillay, Krishnaveni Palaniappan, Neha Varghese, Natalia Mikhailova, Dimitrios Stamatis, T. B. K. Reddy, Chris Daum, Nicole Sh
mBio.2020;[Epub] CrossRef
The Basic-Region Helix-Loop-Helix Transcription Factor DevR Significantly Affects Polysaccharide Metabolism in Aspergillus oryzae
Miao Zhuang, Zhi-Min Zhang, Long Jin, Bao-Teng Wang, Yasuji Koyama, Feng-Jie Jin, Maia Kivisaar
Applied and Environmental Microbiology.2019;[Epub] CrossRef
The Dual-Specificity LAMMER Kinase Affects Stress-Response and Morphological Plasticity in Fungi
Joo-Yeon Lim, Hee-Moon Park
Frontiers in Cellular and Infection Microbiology.2019;[Epub] CrossRef
The APSES transcription factor Vst1 is a key regulator of development in microsclerotium‐ and resting mycelium‐producing Verticillium species
Jorge L. Sarmiento‐Villamil, Nicolás E. García‐Pedrajas, Lourdes Baeza‐Montañez, María D. García‐Pedrajas
Molecular Plant Pathology.2018; 19(1): 59. CrossRef
Essential APSES Transcription Factors for Mycotoxin Synthesis, Fungal Development, and Pathogenicity in Aspergillus flavus
Guangshan Yao, Feng Zhang, Xinyi Nie, Xiuna Wang, Jun Yuan, Zhenhong Zhuang, Shihua Wang
Frontiers in Microbiology.2017;[Epub] CrossRef
A Transcriptome Meta-Analysis Proposes Novel Biological Roles for the Antifungal Protein AnAFP in Aspergillus niger
Norman Paege, Sascha Jung, Paul Schäpe, Dirk Müller-Hagen, Jean-Paul Ouedraogo, Caroline Heiderich, Johanna Jedamzick, Benjamin M. Nitsche, Cees A. van den Hondel, Arthur F. Ram, Vera Meyer, Kap-Hoon Han
PLOS ONE.2016; 11(11): e0165755. CrossRef
Role of LAMMER Kinase in Cell Wall Biogenesis during Vegetative Growth ofAspergillus nidulans
Yu Kyung Choi, Eun-Hye Kang, Hee-Moon Park
Mycobiology.2014; 42(4): 422. CrossRef

Effects of Mutations in the WD40 Domain of α-COP on Its Interaction with the COPI Coatomer in Saccharomyces cerevisiae: Ki-Hyun Kim , Eun Kyung Kim , Ki Young Jeong , Yun-Hee Park , Hee-Moon Park; J. Microbiol. 2012;50(2):256-262. Published online April 27, 2012; DOI: https://doi.org/10.1007/s12275-012-1326-z

45 View
0 Download
4 Scopus

Abstract: Replacement of glycine 227 in the fifth WD40 motif of α-COP/Ret1p/Soo1p by charged or aromatic amino acids is responsible for the temperature-dependent osmo-sensitivity of Saccharomyces cerevisiae, while truncations of WD40 motifs exerted a reduction in cell growth rate and impairment in assembly of cell-wall associated proteins such as enolase and Gas1p. Yeast two-hybrid analysis revealed that the ret1-1/soo1-1 mutation of α-COP abolished the interaction with β- and ε-COP, respectively, and that the interaction between α-COP and β-COP relied on the WD40 domain of α-COP. Furthermore, although the WD40 domain is dispensable for interaction of α-COP with ε-COP, structural alterations in the WD40 domain could impair the interaction.

NOTE] Phycicoccus ochangensis sp. nov., Isolated from Soil of a Potato Cultivation Field: Hyangmi Kim , Hyun-Woo Oh , Doo-Sang Park , Kang Hyun Lee , Sung Uk Kim , Hee-Moon Park , Kyung Sook Bae; J. Microbiol. 2012;50(2):349-353. Published online April 27, 2012; DOI: https://doi.org/10.1007/s12275-012-1206-6

38 View
0 Download
5 Scopus

Abstract: Two novel, Gram-positive, motile, coccal bacteria, strains L1b-b9T and B5a-b5, were isolated from a potato cultivation field in Ochang, Korea. These isolates grew at 10–45°C, pH 5.0–10.0, and in the presence of 8% (w/v) NaCl. The diagnostic diamino acid in the cell-wall peptidoglycan was mesodiaminopimelic acid. The major menaquinone was MK-8(H4) and the main cellular fatty acids were iso-C14:0, iso-C15:0, and anteiso-C15:0. Polar lipids in strain L1b-b9T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and an unknown glyco-amino lipid. The G+C content of genomic DNA was 73.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strains L1b-b9T and B5a-b5 shared 99.36% similarity and formed a robust clade with the type species of the genus Phycicoccus. Strain L1b-b9T is related most closely to Phycicoccus cremeus V2M29T (97.52% 16S rRNA gene sequence similarity). On the basis of phylogenetic characteristics, the name Phycicoccus ochangensis sp. nov. is proposed for strain LIb-b9T (=KCTC 19694T =JCM 17595T).

Validation Study

Generation of Expression Vectors for High-Throughput Functional Analysis of Target Genes in Schizosaccharomyces pombe: Jiwon Ahn , Chung-Hae Choi , Chang-Mo Kang , Chun-Ho Kim , Hee-Moon Park , Kyung-Bin Song , Kwang-Lae Hoe , Misun Won , Kyung-Sook Chung; J. Microbiol. 2009;47(6):789-795. Published online February 4, 2010; DOI: https://doi.org/10.1007/s12275-009-0010-4

46 View
0 Download
6 Crossref

Abstract

An immediate challenge in the post-genomic era is to assign a biological functions to proteins unraveled by genome analysis. This report is based on studies conducted using Schizosaccharomyces pombe, a simple model organism, and presents various vector systems as tools for high-throughput functional analysis of human genes. We constructed S. pombe expression vectors for efficient cloning of genes via the Gateway system. We modified the pREP and pSLF series vectors, which are widely used for gene expression in S. pombe. The vectors constructed have a uniform backbone of S. pombe autonomously replicating sequence (ARS) elements with different selective markers, namely, ura4+ and Saccharomyces cerevisiae LEU2 complementing leu1. These vectors contain 3 different strengths of the inducible promoter nmt1, which affect the expression levels of the cloned open reading frames (ORFs). Further, target proteins can be fused with an N-terminal or C-terminal tag such as triple hemagglutinin (3× HA), enhanced green fluorescent protein (EGFP), or Discosoma red fluorescent protein (DsRed). We tested the feasibility of the constructed vectors by using 3 human genes, namely, RAB18, SCC-112, and PTEN. Proper expression of tagged RAB18 was confirmed by western blot analysis. Further, localization of RAB18, SCC112, and PTEN was demonstrated. The constructed vectors can be utilized for high-throughput functional analysis of heterologous genes.

Citations

Citations to this article as recorded by

Humanized yeast to model human biology, disease and evolution
Aashiq H. Kachroo, Michelle Vandeloo, Brittany M. Greco, Mudabir Abdullah
Disease Models & Mechanisms.2022;[Epub] CrossRef
Reciprocal relation between reporter gene transcription and translation efficiency in fission yeast
Suchita Srivastava, Satinderdeep Kaur, Hemant K. Verma, Suman Rani, Manisha Thakur, Swati Haldar, Jagmohan Singh
Plasmid.2021; 115: 102557. CrossRef
Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics
Bassem Al-Sady, Rachel A. Greenstein, Hana J. El-Samad, Sigurd Braun, Hiten D. Madhani, Juan Mata
PLOS ONE.2016; 11(8): e0159292. CrossRef
Genetic surgery in fungi: employing site-specific recombinases for genome manipulation
Sven Krappmann
Applied Microbiology and Biotechnology.2014; 98(5): 1971. CrossRef
From cradle to grave: high-throughput studies of aging in model organisms
Eric C. Spivey, Ilya J. Finkelstein
Mol. BioSyst..2014; 10(7): 1658. CrossRef
Development of episomal vectors carrying a nourseothricin‐resistance marker for use in minimal media for Schizosaccharomyces pombe
Jiwon Ahn, Misun Won, Mi‐Lang Kyun, Yong Sung Kim, Cho‐Rock Jung, Dong‐Su Im, Kyung‐Bin Song, Kyung‐Sook Chung
Yeast.2013; 30(6): 219. CrossRef

Research Support, Non-U.S. Gov'ts

Paenibacillus camelliae sp. nov., Isolated from Fermented Leaves of Camellia sinensis: Hyun-Woo Oh , Byung-Chun Kim , Kang Hyun Lee , Do Young Kim , Doo-Sang Park , Hee-Moon Park , Kyung Sook Bae; J. Microbiol. 2008;46(5):530-534. Published online October 31, 2008; DOI: https://doi.org/10.1007/s12275-008-0233-9

53 View
0 Download
15 Crossref

Abstract

A novel bacterium, strain b11s-2T was isolated from Pu’er tea. The isolate was Gram-positive, endosporeforming motile rod that grew at 15~42°C and pH 6.0~10.2. The DNA G+C content was 48.3 mol%, the predominant isoprenoid quinone was MK-7, and the predominant cellular fatty acid was anteiso-C15:0 (54.2%) followed by C16:0 (15.5%) and iso-C16:0 (8.2%). The polar lipid pattern of b11s-2T was characterized by the presence of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequence showed that the strain was affiliated within the Paenibacillaceae. The strain was most closely related to Paenibacillus granivorans A30T, with a similarity of 97.1%. Based on the phylogenetic and phenotypic characteristics of strain b11s-2T, the isolate is thought to represent a novel taxon in the genus Paenibacillus. The name Paenibacillus camelliae sp. nov. is proposed for the fermented tea isolate; the type strain is b11s-2T (= KCTC 13220T= CECT 7361T).

Citations

Citations to this article as recorded by

Microbial Succession and Interactions During the Manufacture of Fu Brick Tea
Meichun Xiang, Jun Chu, Wenjiao Cai, Haikun Ma, Weijing Zhu, Xiaoling Zhang, Jinwei Ren, Lizheng Xiao, Dongbo Liu, Xingzhong Liu
Frontiers in Microbiology.2022;[Epub] CrossRef
Production of theophylline via aerobic fermentation of pu-erh tea using tea-derived fungi
Binxing Zhou, Cunqiang Ma, Xiaoying Ren, Tao Xia, Xiaohong Li, Yang Wu
BMC Microbiology.2019;[Epub] CrossRef
Biodegradation of caffeine by whole cells of tea-derived fungi Aspergillus sydowii, Aspergillus niger and optimization for caffeine degradation
Binxing Zhou, Cunqiang Ma, Hongzhen Wang, Tao Xia
BMC Microbiology.2018;[Epub] CrossRef
Paenibacillus oryzae sp. nov., isolated from rice roots
Jun Zhang, Xiao-Tong Ma, Jun-Sheng Gao, Juan-Juan Zhao, Hua-Qun Yin, Cai-Wen Zhang, Rui-Jie Zhang, Xiao-Xia Zhang
International Journal of Systematic and Evolutionary Microbiology .2016; 66(12): 5000. CrossRef
Paenibacillus puernese sp. nov., a β-glucosidase-producing bacterium isolated from Pu’er tea
Dan-Dan Wang, Yeon-Ju Kim, Van-An Hoang, Ngoc-Lan Nguyen, Priyanka Singh, Chao Wang, Deok Chun-Yang
Archives of Microbiology.2016; 198(3): 211. CrossRef
Paenibacillus alba nov., Isolated from Peat Soil
Hyun-Sook Kim, Sathiyaraj Srinivasan, Sang-Seob Lee
Current Microbiology.2015; 70(6): 865. CrossRef
An Integrated Metagenomics/Metaproteomics Investigation of the Microbial Communities and Enzymes in Solid-state Fermentation of Pu-erh tea
Ming Zhao, Dong-lian Zhang, Xiao-qin Su, Shuang-mei Duan, Jin-qiong Wan, Wen-xia Yuan, Ben-ying Liu, Yan Ma, Ying-hong Pan
Scientific Reports.2015;[Epub] CrossRef
Paenibacillus yunnanensis sp. nov., isolated from Pu'er tea
Lili Niu, Tianyi Tang, Zhongliang Ma, Lei Song, Kegui Zhang, Yuanyuan Chen, Ziyi Hua, Xing Hu, Meng Zhao
International Journal of Systematic and Evolutionary Microbiology .2015; 65(Pt_11): 3806. CrossRef
Paenibacillus wenxiniae sp. nov., a nifH gene -harbouring endophytic bacterium isolated from maize
Jun-lian Gao, Fan-yang Lv, Xu-ming Wang, Tian-lei Qiu, Mei Yuan, Ji-wei Li, Yi Zhou, Jian-guang Sun
Antonie van Leeuwenhoek.2015; 108(5): 1015. CrossRef
Paenibacillus guangzhouensis sp. nov., an Fe(III)- and humus-reducing bacterium from a forest soil
Jibing Li, Qin Lu, Ting Liu, Shungui Zhou, Guiqin Yang, Yong Zhao
International Journal of Systematic and Evolutionary Microbiology .2014; 64(Pt_11): 3891. CrossRef
Structure and dynamics of the bacterial communities in fermentation of the traditional Chinese post-fermented pu-erh tea revealed by 16S rRNA gene clone library
Ming Zhao, Wei Xiao, Yan Ma, Tingting Sun, Wenxia Yuan, Na Tang, Donglian Zhang, Yongxia Wang, Yali Li, Hongjie Zhou, Xiaolong Cui
World Journal of Microbiology and Biotechnology.2013; 29(10): 1877. CrossRef
Paenibacillus catalpae sp. nov., isolated from the rhizosphere soil of Catalpa speciosa
Jian Zhang, Zi-Ting Wang, Hui-Min Yu, Yuchao Ma
International Journal of Systematic and Evolutionary Microbiology .2013; 63(Pt_5): 1776. CrossRef
Paenibacillus oceanisediminis sp. nov. isolated from marine sediment
Jina Lee, Na-Ri Shin, Mi-Ja Jung, Seong Woon Roh, Min-Soo Kim, Jung-Sook Lee, Keun Chul Lee, Young-Ok Kim, Jin-Woo Bae
International Journal of Systematic and Evolutionary Microbiology .2013; 63(Pt_2): 428. CrossRef
Paenibacillus xylaniclasticus sp. nov., a xylanolytic-cellulolytic bacterium isolated from sludge in an anaerobic digester
Chakrit Tachaapaikoon, Somboon Tanasupawat, Patthra Pason, Somphit Sornyotha, Rattiya Waeonukul, Khin Lay Kyu, Khanok Ratanakhanokchai
Journal of Microbiology.2012; 50(3): 394. CrossRef
List of new names and new combinations previously effectively, but not validly, published

International Journal of Systematic and Evolutionary Microbiology .2010; 60(11): 2509. CrossRef

Enhanced Secretion of Cell Wall Bound Enolase into Culture Medium by the soo1-1 Mutation of Saccharomyces cerevisiae: Ki-Hyun Kim , Hee-Moon Park; J. Microbiol. 2004;42(3):248-252.; DOI: https://doi.org/2080 [pii]

55 View
0 Download

Abstract: In order to identify the protein(s) secreted into culture medium by the soo1-1/ret1-1 mutation of Saccharomyces cerevisiae, proteins from the culture medium of cells grown at permissive (28^oC) and non-permissive temperatures (37^oC), were analyzed. Comparison of protein bands separated by SDS-PAGE identified a prominent band of 47-kDa band from a mutant grown at 37^oC. N-terminal amino acid sequencing of this 47-kDa protein showed high identity with enolases 1 and 2. Western blot analysis revealed that most of the cell wall-bound enolase was released into the culture medium of the mutant grown at 37^oC, some of which were separated as those with lower molecular weights. Our results, presented here, indicate the impairment of cell wall enolase biogenesis and assembly by the soo1-1/ret1-1 mutation of S. cerevisiae.

Genomic Organization of Penicillium chrysogenum chs4, a Class III Chitin Synthase Gene: Yoon-Dong Park , Myung-Sook Lee , Ji-Hoon Kim , Jun Namgung , Bum Chan Park , Kyung Sook Bae , Hee-Moon Park; J. Microbiol. 2000;38(4):230-238.

52 View
0 Download

Abstract: Class III chitin synthases in filamentous fungi are important for hyphal growth and differentiation of several filamentous fungi. A genomic clone containing the full gene encoding Chs4, a class III chitin synthase in Penicillium chrysogenum, was cloned by PCR screening and colony hybridization from the genomic library. Nucleotide sequence analysis and transcript mapping of chs4 revealed an open reading frame (ORF) that consisted of 5 exons and 4 introns and encoded a putative protein of 915 amino acids. Nucleotide sequence analysis of the 5?lanking region of the ORF revealed a potential TATA box and several binding sites for transcription activators. The putative transcription initiation site at ?6 position was identified by primer extension and the expression of the chs4 during the vegetative growth was confirmed by Northern blot analysis. Amino acid sequence analysis of the Chs4 revealed at least 5 transmembrane helices and several sites for post-translational modifications. Comparison of the amino acid sequence of Chs4 with those of other fungi showed a close relationship between P. chr ysogenum and genus Aspergillus.

Cell Cycle-dependent Expression of Chitin Synthase Genes in Aspergillus nidulans: Bum-Chan Park , Pil-Jae Maeng , Hee-Moon Park; J. Microbiol. 2001;39(1):74-78.

47 View
0 Download

Abstract: The transcription of the chitin synthase genes (chss) was cell cycle-regulated in Aspergillus nidulans and the expression pattern was classified into two groups. Group one, containing chsA and chsC, showed decreasing transcription level upon entry into the S-phase and no further variation during the remainder of the cell cycle. However, group two, containing chsB, chsD, and csmA, showed a sharp decrease of mRNA level upon entry into the G2-phase and an increase during the M-phase. Our results suggested that the chss, belonging to same group with the similar expression pattern during the cell cycle are functionally linked and that chsD may play a role in hyphal growth and development in A. nidulans.

Characterization of Cell Wall Proteins from the soo1-1/ret1-1 Mutant of Saccharomyces cerevisiae: Dong-Won Lee , Ki-Hyun Kim , Se-Chul Chun , Hee-Moon Park; J. Microbiol. 2002;40(3):219-223.

53 View
0 Download

Abstract: In order to investigate the function of Soo1p/[alpha]-COP during post-translational modification and intracellular transport of cell wall proteins in Saccharomyces cerevisiae, cell wall proteins from the soo1-1/ret1-1 mutant cells were analyzed. SDS-PAGE analysis of biotin labeled cell wall proteins suggested that the soo1-1 mutation impairs post-translational modification of cell wall proteins, such as N- and/or O-glycosylation. Analysis of cell wall proteins with antibodies against [beta]-1,3-glucan and [beta]-1,6-glucan revealed alteration of the linkage between cell wall proteins and [beta]-glucans in the soo1-1 mutant cells. Compositional sugar analysis of the cell wall proteins also suggested that the soo1-1 mutation impairs glycosylation of cell wall protein in the ER, which is crucial for the maintenance of cell wall integrity.

Erratum] Phycicoccus ochangensis sp. nov., Isolated from Soil of a Potato Cultivation Field: Hyangmi Kim , Hyun-Woo Oh , Doo-Sang Park , Kang Hyun Lee , Sung Uk Kim , Hee-Moon Park , Kyung Sook Bae Bae; J. Microbiol. 2012;50(5):893-893.

36 View
0 Download

Abstract: In the article by Kim et al. that appears in the Journal of Microbiology 2012; 50, 349-353. KCTC number on page 349 (abstract line 19) and page 353 (left paragraph line 32) should read as KCTC 19695 not KCTC 19694, and on page 353 (left paragraph line 33) should read as KCTC 19694 not KCTC 19695.

First
Prev
Page of 1
Next
Last

Search