Journal of Microbiology

Research Support, Non-U.S. Gov't

Detection of Viruses in Farmed Rainbow Trout (Oncorhynchus mykiss) in Korea by RT-LAMP Assay: Rungkarn Suebsing , Jeong-Ho Kim , Seok Ryel Kim , Myung-Ae Park , Myung-Joo Oh; J. Microbiol. 2011;49(5):741-746. Published online November 9, 2011; DOI: https://doi.org/10.1007/s12275-011-1209-8

Abstract: The viral diseases have been the serious problem in salmonid farming, and rainbow trout is not an exception. In this study, routine surveys were conducted for detecting of viruses in farmed rainbow trout (Oncorhynchus mykiss) in Korea during 2009-2010. Head kidneys from individual fish were employed for virus detection by using a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV) were the target viruses in this study. 53.5% (46/86) were found to be IPNV-positive, while IHNV and VHSV showed RT-LAMP negative during examination for 2 years. Ten IPNV-positive samples were randomly selected for viral isolation and the cells showing CPEs were subjected to RT-LAMP, RT-PCR, and direct sequencing. Phylogenetic analysis showed that the rainbow trout isolate has high similarity homologies with the VR-299 strain, as previously described.

Calcium in infectious hematopoietic necrosis virus (IHNY) infected fish cell lines: Kim, Nam Shik , Heo, Gnag Joon , Lee, Chang Hee; J. Microbiol. 1996;34(3):263-269.

Abstract: Infection of fish cells with IHNV resulted in gradual increase in cytosolic free Ca^2+ concentration ([Ca^2+]) in CHSE, gradual decrease in [Ca^2+] in FHM, and no significant change in RTG cells. The degree of [Ca^2+] increase or decrease was dependent on the amount of infectious virus, and these [Ca^2+] variations were maximal at 16 hours after virus infection (p.i.) in both cell lines. When the fish cells were infected with inactivated IHNV, evident variation in [Ca^2+] was not observed. Thus, infectivity of IHNV appears to correlate with changes in [Ca^2+] in virus-infected cells. These IHNV-induced [Ca^2+] changes were partially blocked by cycloheximide, but not affected by cordycepin. It seems to be that virus-induced Ca^2+ variations were more related with protein synthesis than RNA synthesis. Various Ca^2+ related drugs were used in search for the mechanisms of the [Ca^2+], changes following IHNV infection of CHSE cells. Decreasing extracellular Ca^2+ concentration or blocking Ca^2+ influx from extracellular media inhibited the IHNV-induced increase in [Ca^2+], in CHSE cells. Similar results were obtained with intracellular Ca^2+ sources are important in IHNV-induced [Ca^2+] increase in CHSE cells.

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