Detection of Viruses in Farmed Rainbow Trout (Oncorhynchus mykiss) in Korea by RT-LAMP Assay

Rungkarn Suebsing; Jeong-Ho Kim; Seok Ryel Kim; Myung-Ae Park; Myung-Joo Oh

doi:10.1007/s12275-011-1209-8

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Research Support, Non-U.S. Gov't
Detection of Viruses in Farmed Rainbow Trout (Oncorhynchus mykiss) in Korea by RT-LAMP Assay: Rungkarn Suebsing ¹, Jeong-Ho Kim ¹, Seok Ryel Kim ², Myung-Ae Park ², Myung-Joo Oh ³; Journal of Microbiology 2011;49(5):741-746.
DOI: https://doi.org/10.1007/s12275-011-1209-8
Published online: November 9, 2011

¹Faculty of Marine Bioscience & Technology, Gangneung-Wonju National University, Gangneung 210-702, Republic of Korea, ²National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea, ³Department of Aqualife Medicine, Chonnam National University, Yosu 550-749, Republic of Korea

Corresponding author: Myung-Joo Oh , Tel: +82-61-659-7173,

Received: 21 April 2011 • Accepted: 2 June 2011

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Abstract

The viral diseases have been the serious problem in salmonid farming, and rainbow trout is not an exception. In this study, routine surveys were conducted for detecting of viruses in farmed rainbow trout (Oncorhynchus mykiss) in Korea during 2009-2010. Head kidneys from individual fish were employed for virus detection by using a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV) were the target viruses in this study. 53.5% (46/86) were found to be IPNV-positive, while IHNV and VHSV showed RT-LAMP negative during examination for 2 years. Ten IPNV-positive samples were randomly selected for viral isolation and the cells showing CPEs were subjected to RT-LAMP, RT-PCR, and direct sequencing. Phylogenetic analysis showed that the rainbow trout isolate has high similarity homologies with the VR-299 strain, as previously described.

Keywords: IPNV;IHNV;VHSV;rainbow trout;RT-LAMP

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Detection of Viruses in Farmed Rainbow Trout (Oncorhynchus mykiss) in Korea by RT-LAMP Assay

J. Microbiol. 2011;49(5):741-746. Published online November 9, 2011

DOI: https://doi.org/10.1007/s12275-011-1209-8

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