Detection of Viruses in Farmed Rainbow Trout (Oncorhynchus mykiss) in Korea by RT-LAMP Assay

Rungkarn Suebsing; Jeong-Ho Kim; Seok Ryel Kim; Myung-Ae Park; Myung-Joo Oh

doi:10.1007/s12275-011-1209-8

Articles

Page Path: HOME > J. Microbiol > Volume 49(5); 2011 > Article

Research Support, Non-U.S. Gov't
Detection of Viruses in Farmed Rainbow Trout (Oncorhynchus mykiss) in Korea by RT-LAMP Assay: Rungkarn Suebsing ¹, Jeong-Ho Kim ¹, Seok Ryel Kim ², Myung-Ae Park ², Myung-Joo Oh ³; Journal of Microbiology 2011;49(5):741-746.
DOI: https://doi.org/10.1007/s12275-011-1209-8
Published online: November 9, 2011

¹Faculty of Marine Bioscience & Technology, Gangneung-Wonju National University, Gangneung 210-702, Republic of Korea, ²National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea, ³Department of Aqualife Medicine, Chonnam National University, Yosu 550-749, Republic of Korea

Corresponding author: Myung-Joo Oh , Tel: +82-61-659-7173,

Received: 21 April 2011 • Accepted: 2 June 2011

prev next

239 Views
0 Download
7 Crossref
10 Scopus

Full Article

Download PDF

Abstract

The viral diseases have been the serious problem in salmonid farming, and rainbow trout is not an exception. In this study, routine surveys were conducted for detecting of viruses in farmed rainbow trout (Oncorhynchus mykiss) in Korea during 2009-2010. Head kidneys from individual fish were employed for virus detection by using a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV) were the target viruses in this study. 53.5% (46/86) were found to be IPNV-positive, while IHNV and VHSV showed RT-LAMP negative during examination for 2 years. Ten IPNV-positive samples were randomly selected for viral isolation and the cells showing CPEs were subjected to RT-LAMP, RT-PCR, and direct sequencing. Phylogenetic analysis showed that the rainbow trout isolate has high similarity homologies with the VR-299 strain, as previously described.

Keywords: IPNV;IHNV;VHSV;rainbow trout;RT-LAMP

Figure & Data

References

Citations

Citations to this article as recorded by

The development and initial evaluation of a multiplex RT-PCR and high-throughput liquid chip assay for the simultaneous detection of five salmonid virus diseases
Yu Chen, Shuai Gao, Hao Xu, Songqi Zhu, Guixiang Tong, Xiaoyu Chen, Xinxian Wei, Hong Liu, Xiaocong Zheng
Aquaculture Reports.2024; 36: 102163. CrossRef
Comprehensive review on infectious pancreatic necrosis virus
Irtifa Fayaz, Raja Aadil Hussain Bhat, Ritesh Shantilal Tandel, Pragyan Dash, Suresh Chandra, Maneesh Kumar Dubey, Parvaiz Ahmad Ganie
Aquaculture.2023; 574: 739737. CrossRef
A brief review of novel nucleic acid test biosensors and their application prospects for salmonids viral diseases detection
Xiaofei Liu, Songyin Qiu, Haiping Fang, Lin Mei, Hongli Jing, Chunyan Feng, Shaoqiang Wu, Xiangmei Lin
Journal of Semiconductors.2023; 44(2): 023103. CrossRef
Piscine orthoreovirus: Biology and distribution in farmed and wild fish
Mark P. Polinski, Niccoló Vendramin, Argelia Cuenca, Kyle A. Garver
Journal of Fish Diseases.2020; 43(11): 1331. CrossRef
Infectious hematopoietic necrosis virus: advances in diagnosis and vaccine development
Chean Yeah Yong, Hui Kian Ong, Hooi Chia Tang, Swee Keong Yeap, Abdul Rahman Omar, Kok Lian Ho, Wen Siang Tan
PeerJ.2019; 7: e7151. CrossRef
Loop-mediated isothermal amplification (LAMP) assays for detection and identification of aquaculture pathogens: current state and perspectives
Gouranga Biswas, Masahiro Sakai
Applied Microbiology and Biotechnology.2014; 98(7): 2881. CrossRef
A one‐step molecular biology method for simple and rapid detection of grass carp Ctenopharyngodon idella reovirus (GCRV) HZ08 strain
W. W. Zeng, Q. Wang, Y. Y. Wang, D. H. Xu, S. Q. Wu
Journal of Fish Biology.2013; 82(5): 1545. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Detection of Viruses in Farmed Rainbow Trout (Oncorhynchus mykiss) in Korea by RT-LAMP Assay

J. Microbiol. 2011;49(5):741-746. Published online November 9, 2011

DOI: https://doi.org/10.1007/s12275-011-1209-8

Related articles