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- An ethanol extract of Lysimachia mauritiana exhibits inhibitory activity against hepatitis E virus genotype 3 replication
- Seong Eun Jin , Jung-Eun Kim , Sun Yeou Kim , Bang Ju Park , Yoon-Jae Song
- J. Microbiol. 2017;55(12):984-988. Published online December 7, 2017
- DOI: https://doi.org/10.1007/s12275-017-7477-1
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Abstract
- Hepatitis E virus (HEV) is an etiological agent of acute hepatitis E, a self-limiting disease prevalent in developing countries. HEV can cause fulminant hepatic failure with high mortality rates in pregnant women, and genotype 3 is reported to trigger chronic hepatitis in immunocompromised individuals worldwide. Screening of plant extracts for compounds with potential anti-HEV effects led to the identification of a 70% ethanol extract of Lysimachia mauritiana (LME) that interferes with replication of the swine HEV genotype 3 replicon. Furthermore, LME significantly inhibited replication of HEV genotype 3 and expression of HEV ORF2 in infected cells without exerting cytotoxic effects. Collectively, our findings demonstrate the potential utility of LME in the development of novel antiviral drugs against HEV infection.
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Citations
Citations to this article as recorded by
- Host-targeting antivirals for chronic viral infections of the liver
Nicola Frericks, Mara Klöhn, Frauke Lange, Lilli Pottkämper, Arnaud Carpentier, Eike Steinmann
Antiviral Research.2025; 234: 106062. CrossRef - Seedling Growth of Lysimachia mauritiana Lam. by Cell Size and Nutrient Solution Concentration
Eun Ji Choi, Jun Young Kim, Gyeong Ho Jang, Soo Hoon Kim, Sang Im Oh, Hong Min Koh, SiU Hong, Mijeong Yoon, Chung Ho Ko
Flower Research Journal.2024; 32(2): 103. CrossRef - Lysimachia mauritiana Lam. Extract Alleviates Airway Inflammation Induced by Particulate Matter Plus Diesel Exhaust Particles in Mice
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European Journal of Integrative Medicine.2024; 72: 102419. CrossRef - HPLC Analysis of Polyphenolic Compounds in Lysimachia nummularia L. and Comparative Determination of Antioxidant Capacity
Felicia Suciu, Iuliana Stoicescu, Elena Lupu, Adina Musuc, Antoanela Popescu, Magdalena Mititelu, Adrian Roșca, Denisa-Elena Dumitrescu, Florin Badea, Aureliana Caraiane, Victoria Badea
Applied Sciences.2023; 13(4): 2159. CrossRef - Hepatitis E Virus Infection: Circulation, Molecular Epidemiology, and Impact on Global Health
Srinivas Reddy Pallerla, Dominik Harms, Reimar Johne, Daniel Todt, Eike Steinmann, Mathias Schemmerer, Jürgen J. Wenzel, Jörg Hofmann, James Wai Kuo Shih, Heiner Wedemeyer, C.-Thomas Bock, Thirumalaisamy P. Velavan
Pathogens.2020; 9(10): 856. CrossRef - Hepatitis E Virus Drug Development
Volker Kinast, Thomas L Burkard, Daniel Todt, Eike Steinmann
Viruses.2019; 11(6): 485. CrossRef
- Host-targeting antivirals for chronic viral infections of the liver
- Expression of Human Protease Inhibitor Nexin-I in Escherichia coli
- Ha, Sang Deuk , Kim, Ji Ha , Park, Hey Lyoun , Hyun, Hyung Hwan , Chung, Hyung Min , Kim Kwon, Yun Hee , Seo, Seong Yum , Ko, Jung Jae , Lee, Hyun Hwan
- J. Microbiol. 1998;36(4):283-288.
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Abstract
- Human protease inhibitor nexin-I(NX-I) cDNA(1.2Kb) was isolated from human lung cDNA library and expressed under the control of T7 promoter as a fused protein in Escherichia coli BL21 and E. coli GJ1158 by addition of IPTG and Nacl as inducers. For GJ1158, 300 mM NaCl was added for induction after the cell reached A_600=0.6. As a result, E. coli GJ1158 showed higher expression level than BL2l with lesser extent of inclusion bodies. The optimum concentration of NaCl exerting no induction effect but shortening the time to reach A_600=0.6 was 50 mM. All the results suggested that E. coli GJ1158 was a useful host for efficient expression of NX-I using NaCl as an inducer. The expressed NX-1 showed an inhibitory effect on thrombin activity. The expressed protein was purified by immobilized metal affinity column chromatography (IMAC) and characterized by digestion with enterokinase (EK).
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