Journal of Microbiology

Research Article

LasB activation in Pseudomonas aeruginosa: Quorum sensing-mediated release of an auto-activation inhibitor: Cheol Seung Lee, Xi-Hui Li, Chae-Ran Jeon, Joon-Hee Lee; J. Microbiol. 2025;63(2):e2411005. Published online February 27, 2025; DOI: https://doi.org/10.71150/jm.2411005

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Abstract PDF: Pseudomonas aeruginosa secretes three major proteases: elastase B (LasB), protease IV (PIV), and elastase A (LasA), which play crucial roles in infection and pathogenesis. These proteases are activated sequentially from LasB in a proteolytic cascade, and LasB was previously thought to undergo auto-activation. However, our previous study suggested that LasB cannot auto-activate independently but requires additional quorum sensing (QS)-dependent factors for activation, as LasB remained inactive in QS-deficient P. aeruginosa (QS^-) even under artificial overexpression. In this study, we provide evidence for the existence of a LasB inhibitor in QS^- mutants: inactive LasB overexpressed in QS^- strains was in its processed form and could be reactivated upon purification; when full-length LasB was overexpressed in Escherichia coli, a heterologous bacterium lacking both LasB activators and inhibitors, the protein underwent normal processing and activation; and purified active LasB was significantly inhibited by culture supernatant (CS) from QS^- strains but not by CS from QS⁺ strains. These findings demonstrate that a LasB inhibitor exists in QS^- strains, and in its absence, LasB can undergo auto-activation without requiring an activator. Based on these results, we propose an updated hypothesis: the QS-dependent LasB activator functions by removing the LasB inhibitor rather than acting directly on LasB itself, thus preventing premature LasB activation until QS response is initiated.

Journal Articles

Application of fast expectation-maximization microbial source tracking to discern fecal contamination in rivers exposed to low fecal inputs: Youfen Xu , Ganghua Han , Hongxun Zhang , Zhisheng Yu , Ruyin Liu; J. Microbiol. 2022;60(6):594-601. Published online April 18, 2022; DOI: https://doi.org/10.1007/s12275-022-1651-9

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Community-based microbial source tracking (MST) can be used to determine fecal contamination from multiple sources in the aquatic environment. However, there is little scientific information on its application potential in water environmental management. Here, we compared SourceTracker and Fast Expectation-maximization Microbial Source Tracking (FEAST) performances on environmental water bodies exposed to low fecal pollution and evaluated treatment effects of fecal pollution in the watershed utilizing community-based MST. Our results showed that FEAST overall outperformed SourceTracker in sensitivity and stability, and was able to discern multi-source fecal contamination (mainly chicken feces) in ambient water bodies exposed to low fecal inputs. Consistent with our previous PCR/qPCR-based MST assays, FEAST analysis indicates that fecal pollution has been significantly mitigated through comprehensive environmental treatment by the local government. This study suggests that FEAST can be a powerful tool for accurately evaluating the contribution of multi-source fecal contamination in environmental water, facilitating environmental management.

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Faecal source apportionment using molecular methods: A proof of concept using the FEAST algorithm
Laura T. Kelly, Jack Sissons, Lucy Thompson, John K. Pearman
Water Research.2024; 266: 122365. CrossRef
Novel Microbial Engraftment Trajectories Following Microbiota Transplant Therapy in Ulcerative Colitis
Daphne Moutsoglou, Aneesh Syal, Sharon Lopez, Elizabeth C Nelson, Lulu Chen, Amanda J Kabage, Monika Fischer, Alexander Khoruts, Byron P Vaughn, Christopher Staley
Journal of Crohn's and Colitis.2024;[Epub] CrossRef
Computational methods and challenges in analyzing intratumoral microbiome data
Qi Wang, Zhaoqian Liu, Anjun Ma, Zihai Li, Bingqiang Liu, Qin Ma
Trends in Microbiology.2023; 31(7): 707. CrossRef
Response and recovery mechanisms of river microorganisms to gradient concentrations of estrogen
Dan Qin, Yan Li, Nengwang Chen, Anyi Hu, Chang-Ping Yu
Frontiers in Microbiology.2023;[Epub] CrossRef
Improving the Identification of Fecal Contamination in Recreational Water through the Standardization and Normalization of Microbial Source Tracking
Megan N. Jamison, John J. Hart, David C. Szlag
ACS ES&T Water.2022; 2(12): 2305. CrossRef

Regulator of ribonuclease activity modulates the pathogenicity of Vibrio vulnificus: Jaejin Lee , Eunkyoung Shin , Jaeyeong Park , Minho Lee , Kangseok Lee; J. Microbiol. 2021;59(12):1133-1141. Published online November 9, 2021; DOI: https://doi.org/10.1007/s12275-021-1518-5

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Abstract

RraA, a protein regulator of RNase E activity, plays a unique role in modulating the mRNA abundance in Escherichia coli. The marine pathogenic bacterium Vibrio vulnificus also possesses homologs of RNase E (VvRNase E) and RraA (VvRraA1 and VvRraA2). However, their physiological roles have not yet been investigated. In this study, we demonstrated that VvRraA1 expression levels affect the pathogenicity of V. vulnificus. Compared to the wild-type strain, the VvrraA1-deleted strain (ΔVvrraA1) showed decreased motility, invasiveness, biofilm formation ability as well as virulence in mice; these phenotypic changes of ΔVvrraA1 were restored by the exogenous expression of VvrraA1. Transcriptomic analysis indicated that VvRraA1 expression levels affect the abundance of a large number of mRNA species. Among them, the halflives of mRNA species encoding virulence factors (e.g., smcR and htpG) that have been previously shown to affect VvrraA1 expression-dependent phenotypes were positively correlated with VvrraA1 expression levels. These findings suggest that VvRraA1 modulates the pathogenicity of V. vulnificus by regulating the abundance of a subset of mRNA species.

Citations

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Identification of the global regulatory roles of RraA via the integrative transcriptome and proteome in Vibrio alginolyticus
Huizhen Chen, Qian Gao, Bing Liu, Ying Zhang, Jianxiang Fang, Songbiao Wang, Youqi Chen, Chang Chen, Nicolas E. Buchler
mSphere.2024;[Epub] CrossRef
Comparative Transcriptomic Analysis of Flagellar-Associated Genes in Salmonella Typhimurium and Its rnc Mutant
Seungmok Han, Ji-Won Byun, Minho Lee
Journal of Microbiology.2024; 62(1): 33. CrossRef
Eco-Evolutionary Drivers of Vibrio parahaemolyticus Sequence Type 3 Expansion: Retrospective Machine Learning Approach
Amy Marie Campbell, Chris Hauton, Ronny van Aerle, Jaime Martinez-Urtaza
JMIR Bioinformatics and Biotechnology.2024; 5: e62747. CrossRef
Relaxed Cleavage Specificity of Hyperactive Variants of Escherichia coli RNase E on RNA I
Dayeong Bae, Hana Hyeon, Eunkyoung Shin, Ji-Hyun Yeom, Kangseok Lee
Journal of Microbiology.2023; 61(2): 211. CrossRef
Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef

iTRAQ-facilitated proteomic analysis of Bacillus cereus via degradation of malachite green: Bobo Wang , Jing Lu , Junfang Zheng , Zhisheng Yu; J. Microbiol. 2021;59(2):142-150. Published online February 1, 2021; DOI: https://doi.org/10.1007/s12275-021-0441-0

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Abstract

The wide use of malachite green (MG) as a dye has caused substantial concern owing to its toxicity. Bacillus cereus can against the toxic effect of MG and efficiently decolourise it. However, detailed information regarding its underlying adaptation and degradation mechanisms based on proteomic data is scarce. In this study, the isobaric tags for relative and absolute quantitation (iTRAQ)-facilitated quantitative method was applied to analyse the molecular mechanisms by which B. cereus degrades MG. Based on this analysis, 209 upregulated proteins and 198 downregulated proteins were identified with a false discovery rate of 1% or less during MG biodegradation. Gene ontology and KEGG analysis determined that the differentially expressed proteins were enriched in metabolic processes, catalytic activity, antioxidant activity, and responses to stimuli. Furthermore, real-time qPCR was utilised to further confirm the regulated proteins involved in benzoate degradation. The proteins BCE_4076 (Acetyl-CoA acetyltransferase), BCE_5143 (Acetyl-CoA acetyltransferase), BCE_5144 (3-hydroxyacyl-CoA dehydrogenase), BCE_4651 (Enoyl-CoA hydratase), and BCE_5474 (3-hydroxyacyl-CoA dehydrogenase) involved in the benzoate degradation pathway may play an important role in the biodegradation of MG by B. cereus. The results of this study not only provide a comprehensive view of proteomic changes in B. cereus upon MG loading but also shed light on the mechanism underlying MG biodegradation by B. cereus.

Citations

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Engineering globins for efficient biodegradation of malachite green: two case studies of myoglobin and neuroglobin
Jiao Liu, Jia-Kun Xu, Hong Yuan, Xiao-Juan Wang, Shu-Qin Gao, Ge-Bo Wen, Xiang-Shi Tan, Ying-Wu Lin
RSC Advances.2022; 12(29): 18654. CrossRef

The putative polysaccharide synthase AfCps1 regulates Aspergillus fumigatus morphogenesis and conidia immune response in mouse bone marrow-derived macrophages: Sha Wang , Anjie Yuan , Liping Zeng , Sikai Hou , Meng Wang , Lei Li , Zhendong Cai , Guowei Zhong; J. Microbiol. 2021;59(1):64-75. Published online November 17, 2020; DOI: https://doi.org/10.1007/s12275-021-0347-x

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Abstract

Aspergillus fumigatus is a well-known opportunistic pathogen that causes invasive aspergillosis (IA) infections with high mortality in immunosuppressed individuals. Morphogenesis, including hyphal growth, conidiation, and cell wall biosynthesis is crucial in A. fumigatus pathogenesis. Based on a previous random insertional mutagenesis library, we identified the putative polysaccharide synthase gene Afcps1 and its paralog Afcps2. Homologs of the cps gene are commonly found in the genomes of most fungal and some bacterial pathogens. Afcps1/cpsA is important in sporulation, cell wall composition, and virulence. However, the precise regulation patterns of cell wall integrity by Afcps1/cpsA and further effects on the immune response are poorly understood. Specifically, our in-depth study revealed that Afcps1 affects cell-wall stability, showing an increased resistance of ΔAfcps1 to the chitinmicrofibril destabilizing compound calcofluor white (CFW) and susceptibility of ΔAfcps1 to the β-(1,3)-glucan synthase inhibitor echinocandin caspofungin (CS). Additionally, deletion of Afcps2 had a normal sporulation phenotype but caused hypersensitivity to Na+ stress, CFW, and Congo red (CR). Specifically, quantitative analysis of cell wall composition using high-performance anion exchange chromatography- pulsed amperometric detector (HPAEC-PAD) analysis revealed that depletion of Afcps1 reduced cell wall glucan and chitin contents, which was consistent with the downregulation of expression of the corresponding biosynthesis genes. Moreover, an elevated immune response stimulated by conidia of the ΔAfcps1 mutant in marrow-derived macrophages (BMMs) during phagocytosis was observed. Thus, our study provided new insights into the function of polysaccharide synthase Cps1, which is necessary for the maintenance of cell wall stability and the adaptation of conidia to the immune response of macrophages in A. fumigatus.

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Study on the metabolic changes and regulatory mechanism of Aspergillus flavus conidia germination
Sifan Jia, Chong Li, Yu An, Desheng Qi, Erik F. Y. Hom
Microbiology Spectrum.2024;[Epub] CrossRef
Chitin Biosynthesis in Aspergillus Species
Veronica S. Brauer, André M. Pessoni, Mateus S. Freitas, Marinaldo P. Cavalcanti-Neto, Laure N. A. Ries, Fausto Almeida
Journal of Fungi.2023; 9(1): 89. CrossRef
Evidencing New Roles for the Glycosyl-Transferase Cps1 in the Phytopathogenic Fungus Botrytis cinerea
Matthieu Blandenet, Isabelle R. Gonçalves, Christine Rascle, Jean-William Dupuy, François-Xavier Gillet, Nathalie Poussereau, Mathias Choquer, Christophe Bruel
Journal of Fungi.2022; 8(9): 899. CrossRef

Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulatessensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology: Kui Wu , Yangyun Zheng , Qingping Wu , Haiying Chen , Songzhe Fu , Biao Kan , Yongyan Long , Xiansheng Ni , Junling Tu; J. Microbiol. 2019;57(12):1105-1114. Published online November 4, 2019; DOI: https://doi.org/10.1007/s12275-019-9379-x

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Abstract

In order to adapt to different environments, Vibrio parahaemolyticus employed a complicated quorum sensing system to orchestrate gene expression and diverse colony morphology patterns. In this study, the function of the putative quorum sensing signal synthase gene cqsA (VPA0711 in V. parahaemolyticus strain RIMD2210633 genome) was investigated. The cloning and expression of V. parahaemolyticus cqsA in Escherichia coli system induced the production of a new quorum sensing signal that was found in its culture supernatant. The signal was purified by high performance liquid chromatography
methods
and determined to be 3-hydroxyundecan- 4-one by indirect and direct mass spectra assays. The deletion of cqsA in RIMD2210633 changed V. parahaemolyticus colony morphology from the classical ‘fried-egg’ shape (thick and opaque in the center, while thin and translucent in the edge) of the wild-type colony to a ‘pancake’ shape (no significant difference between the centre and the edge) of the cqsAdeleted colony. This morphological change could be restored by complementary experiment with cqsA gene or the signal extract. In addition, the expression of opaR, a well-known quorum sensing regulatory gene, could be up-regulated by cqsA deletion. Our results suggested that V. parahaemolyticus used cqsA to produce 3-hydroxyundecan-4-one signal and thereby regulated colony morphology and other quorum sensing-associated behaviors.

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Antimicrobial resistance, virulence factors and phylogenetic profiles of Vibrio parahaemolyticus in the eastern coast of Shenzhen
Xian Qiang Lian, Guo Dong Liu, Miao Fen Huang, Qiu Hua Fan, Zi Dan Lin
Frontiers in Microbiology.2024;[Epub] CrossRef
Quorum sensing signal synthases enhance Vibrio parahaemolyticus swarming motility
Fuwen Liu, Fei Wang, Yixuan Yuan, Xiaoran Li, Xiaojun Zhong, Menghua Yang
Molecular Microbiology.2023; 120(2): 241. CrossRef
Regulation of Virulence Factors Expression During the Intestinal Colonization of Vibrio parahaemolyticus
Jingyu Wang, Yuming Zhan, Han Sun, Xiaodan Fu, Qing Kong, Changliang Zhu, Haijin Mou
Foodborne Pathogens and Disease.2022; 19(3): 169. CrossRef
Supplementation of ex situ produced bioflocs improves immune response against AHPND in Pacific whiteleg shrimp (Litopenaeus vannamei) postlarvae
Magdalena Lenny Situmorang, Umaporn Uawisetwathana, Sopacha Arayamethakorn, Nitsara Karoonuthaisiri, Wanilada Rungrassamee, Haniswita Haniswita, Peter Bossier, Gede Suantika
Applied Microbiology and Biotechnology.2022; 106(9-10): 3751. CrossRef
A novel finding of intra-genus inhibition of quorum sensing in Vibrio bacteria
Huong Thanh Hoang, Thuy Thu Thi Nguyen, Ha Minh Do, Thao Kim Nu Nguyen, Hai The Pham
Scientific Reports.2022;[Epub] CrossRef
CqsA-introduced quorum sensing inhibits type VI secretion system 2 through an OpaR-dependent pathway in Vibrio parahaemolyticus
Kui Wu, Yongyan Long, Qian Liu, Wei Wang, Guoyin Fan, Hui Long, Yangyun Zheng, Xiansheng Ni, Shengen Chen, Haiying Chen, Shufen Shuai
Microbial Pathogenesis.2022; 162: 105334. CrossRef
CqsA inhibits the virulence of Vibrio harveyi to the pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)
Yaqiu Zhang, Yiqin Deng, Juan Feng, Zhixun Guo, Can Mao, Haoxiang Chen, Ziyang Lin, Jianmei Hu, Youlu Su
Aquaculture.2021; 535: 736346. CrossRef
Identification of LuxR Family Regulators That Integrate Into Quorum Sensing Circuit in Vibrio parahaemolyticus
Xiaojun Zhong, Ranran Lu, Fuwen Liu, Jinjie Ye, Junyang Zhao, Fei Wang, Menghua Yang
Frontiers in Microbiology.2021;[Epub] CrossRef
Adaptations of Vibrio parahaemolyticus to Stress During Environmental Survival, Host Colonization, and Infection
Gururaja Perumal Pazhani, Goutam Chowdhury, Thandavarayan Ramamurthy
Frontiers in Microbiology.2021;[Epub] CrossRef
Vibrio alginolyticus influences quorum sensing-controlled phenotypes of acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus
Panida Paopradit, Natta Tansila, Komwit Surachat, Pimonsri Mittraparp-arthorn
PeerJ.2021; 9: e11567. CrossRef
Dynamics and Microevolution of Vibrio parahaemolyticus Populations in Shellfish Farms
Songzhe Fu, Qingyao Wang, Yixiang Zhang, Qian Yang, Jingwei Hao, Ying Liu, Bo Pang, Michael S. Rappe
mSystems.2021;[Epub] CrossRef

Two novel synthetic peptides inhibit quorum sensing-dependent biofilm formation and some virulence factors in Pseudomonas aeruginosa PAO1: Mostafa N. Taha , Amal E. Saafan , A. Ahmedy , Eman El Gebaly , Ahmed S. Khairalla; J. Microbiol. 2019;57(7):618-625. Published online June 27, 2019; DOI: https://doi.org/10.1007/s12275-019-8548-2

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Abstract

Quorum sensing (QS) regulates virulence factor expression in Pseudomonas aeruginosa. Inhibiting the QS-controlled virulence factors without inhibiting the growth of P. aeruginosa is a promising approach for overcoming the widespread resistance of P. aeruginosa. This study was proposed to investigate the effects of two novel synthetic peptides on the biofilm development and virulence factor production of P. aeruginosa. The tested strain was P. aeruginosa PAO1. The results indicated that both of the synthetic peptides (LIVRHK and LIVRRK) inhibited (P < 0.05) the formation of biofilms and the production of virulence factors, including pyocyanin, protease, and rhamnolipids, without inhibiting the growth of PAO1. Additionally, we detected transcriptional changes related to QS and found a significant reduction in the levels of gene expression of lasI, lasR, rhlI, and rhlR. This study demonstrates that LIVRRK and LIVRHK are novel synthetic peptides that can act as potent inhibitors of QS-regulated virulence factors in P. aeruginosa. Moreover, these synthetic peptides have potential applications in the treatment of biofilmrelated diseases. Both peptides may be able to control chronic infections and biofilm-associated problems of P. aeruginosa.

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Antibiofilm activity of marine microbial natural products: potential peptide- and polyketide-derived molecules from marine microbes toward targeting biofilm-forming pathogens
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A Systematic Hierarchical Virtual Screening Model for RhlR Inhibitors Based on PCA, Pharmacophore, Docking, and Molecular Dynamics
Jiarui Du, Jiahao Li, Juqi Wen, Jun Liu, Haichuan Xiao, Antian Zhang, Dongdong Yang, Pinghua Sun, Haibo Zhou, Jun Xu
International Journal of Molecular Sciences.2024; 25(14): 8000. CrossRef
Antimicrobial peptides fight against Pseudomonas aeruginosa at a sub-inhibitory concentration via anti-QS pathway
Li Li, Jiaxin Li, Xiaodan Yu, Ruipin Cao, Meiling Hong, Zuxian Xu, Jian Ren Lu, Yinglu Wang, Hu Zhu
Bioorganic Chemistry.2023; 141: 106922. CrossRef
The Role of Quorum Sensing Molecules in Bacterial–Plant Interactions
Jan Majdura, Urszula Jankiewicz, Agnieszka Gałązka, Sławomir Orzechowski
Metabolites.2023; 13(1): 114. CrossRef
Peptide LQLY3-1, a novel Vibrio harveyi quorum sensing inhibitor produced by Lactococcus lactis LY3-1
Yangrui Wang, Mengtong Sun, Xiaoling Cui, Yongyue Gao, Xinran Lv, Jianrong Li, Fengling Bai, Xuepeng Li, Defu Zhang, Kai Zhou
LWT.2022; 170: 114093. CrossRef
Design and assessment of novel synthetic peptides to inhibit quorum sensing-dependent biofilm formation in Pseudomonas aeruginosa
Fatemeh Aflakian, Mehrnaz Rad, Gholamreza Hashemitabar, Milad Lagzian, Mohammad Ramezani
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Zoya Peerzada, Ashish M. Kanhed, Krutika B. Desai
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A Bacterial Isolate Capable of Quenching Both Diffusible Signal Factor- and N-Acylhomoserine Lactone-Family Quorum Sensing Signals Shows Much Enhanced Biocontrol Potencies
Huishan Wang, Qiqi Lin, Lingling Dong, Wenting Wu, Zhibing Liang, Zhangyong Dong, Huijuan Ye, Lisheng Liao, Lian-Hui Zhang
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Jyoti Vishwakarma, Bhumika Waghela, Berness Falcao, Sirisha L. Vavilala
Applied Biochemistry and Biotechnology.2022; 194(2): 671. CrossRef
Molecular Characteristics, Antimicrobial Resistance, and Biofilm Formation of Pseudomonas aeruginosa Isolated from Patients with Aural Infections in Shanghai, China
Feifei Yang, Chunhong Liu, Jian Ji, Wenjun Cao, Baixing Ding, Xiaogang Xu
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Molecular engineering of antimicrobial peptides: microbial targets, peptide motifs and translation opportunities
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Type VI secretion system of Pseudomonas aeruginosa is associated with biofilm formation but not environmental adaptation
Lihua Chen, Yaru Zou, Asmaa Abbas Kronfl, Yong Wu
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A common evolutionary pathway for maintaining quorum sensing in Pseudomonas aeruginosa: Bai-min Lai , Hui-cong Yan , Mei-zhen Wang , Na Li , Dong-sheng Shen; J. Microbiol. 2018;56(2):83-89. Published online February 2, 2018; DOI: https://doi.org/10.1007/s12275-018-7286-1

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Abstract

In the bacterium Pseudomonas aeruginosa, the synthesis and secretion of extracellular protease is a typical cooperative behavior regulated by quorum sensing. However, this type of cooperative behavior is easily exploited by other individuals who do not synthesize public goods, which is known as the “tragedy of the commons”. Here P. aeruginosa was inoculated into casein media with different nitrogen salts added. In casein broth, protease (a type of public good) is necessary for bacterial growth. After 30 days of sequential transfer, some groups propagated stably and avoided “tragedy of the commons”. The evolved cooperators who continued to synthesize protease were isolated from these stable groups. By comparing the characteristics of quorum sensing in these cooperators, an identical evolutionary pattern was found. A variety of cooperative behaviors regulated by quorum sensing, such as the synthesis and secretion of protease and signals, were significantly reduced during the process of evolution. Such reductions improved the efficiency of cooperation, helping to prevent cheating. In addition, the production of pyocyanin, which is regulated by the RhlIR system, increased during the process of evolution, possibly due to its role in stabilizing the cooperation. This study contributes towards our understanding of the evolution of quorum sensing of P. aeruginosa.

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Diesel degradation capability and environmental robustness of strain Pseudomonas aeruginosa WS02
Penghong Luo, Yankui Tang, Jiahua Lu, Lu Jiang, Yiting Huang, Qiming Jiang, Xuemin Chen, Tianfu Qin, Holly Alice Shiels
Journal of Environmental Management.2024; 351: 119937. CrossRef
Biofilm control on metallic materials in medical fields from the viewpoint of materials science – from the fundamental aspects to evaluation
Hideyuki Kanematsu, Dana M. Barry, Hajime Ikegai, Yoshimitsu Mizunoe
International Materials Reviews.2023; 68(3): 247. CrossRef
To cheat or not to cheat: cheatable and non-cheatable virulence factors in Pseudomonas aeruginosa
Katya Dafne Guadarrama-Orozco, Caleb Perez-Gonzalez, Kokila Kota, Miguel Cocotl-Yañez, Jesús Guillermo Jiménez-Cortés, Miguel Díaz-Guerrero, Mariel Hernández-Garnica, Julia Munson, Frederic Cadet, Luis Esaú López-Jácome, Ángel Yahir Estrada-Velasco, Ana M
FEMS Microbiology Ecology.2023;[Epub] CrossRef
Exoprotease exploitation and social cheating in a Pseudomonas aeruginosa environmental lysogenic strain with a noncanonical quorum sensing system
Daniel Huelgas-Méndez, Daniel Cazares, Luis David Alcaraz, Corina Diana Ceapã, Miguel Cocotl-Yañez, Toya Shotaro, Toshinari Maeda, Ana María Fernández-Presas, Oswaldo Tostado-Islas, Ana Lorena González-Vadillo, Aldo Limones-Martínez, Carlos Eduardo Hernan
FEMS Microbiology Ecology.2023;[Epub] CrossRef
3-Phenylpropan-1-Amine Enhanced Susceptibility of Serratia marcescens to Ofloxacin by Occluding Quorum Sensing
Lujun Yin, Ping-Ping Zhang, Wei Wang, Shi Tang, Shi-Ming Deng, Ai-Qun Jia, Gyanu Lamichhane
Microbiology Spectrum.2022;[Epub] CrossRef
A deep insight into the suppression mechanism of Sedum alfredii root exudates on Pseudomonas aeruginosa based on quorum sensing
Min Zhu, Yusheng Yang, Meizhen Wang, Xiaoxiao Li, Ruifang Han, Qianqian Chen, Dongsheng Shen, Jiali Shentu
Ecotoxicology and Environmental Safety.2021; 217: 112240. CrossRef
Tobramycin Adaptation Enhances Policing of Social Cheaters in Pseudomonas aeruginosa
Rhea G. Abisado, John H. Kimbrough, Brielle M. McKee, Vaughn D. Craddock, Nicole E. Smalley, Ajai A. Dandekar, Josephine R. Chandler, Rebecca E. Parales
Applied and Environmental Microbiology.2021;[Epub] CrossRef
Quercus infectoria gall extracts reduce quorum sensing-controlled virulence factors production and biofilm formation in Pseudomonas aeruginosa recovered from burn wounds
Akhter Ahmed Ahmed, Fraidoon Abdulqadir Salih
BMC Complementary and Alternative Medicine.2019;[Epub] CrossRef
Seeding Public Goods Is Essential for Maintaining Cooperation in Pseudomonas aeruginosa
Daniel Loarca, Dánae Díaz, Héctor Quezada, Ana Laura Guzmán-Ortiz, Abril Rebollar-Ruiz, Ana María Fernández Presas, Jimena Ramírez-Peris, Rafael Franco-Cendejas, Toshinari Maeda, Thomas K. Wood, Rodolfo García-Contreras
Frontiers in Microbiology.2019;[Epub] CrossRef
Pyocyanin Restricts Social Cheating in Pseudomonas aeruginosa
Paulina Castañeda-Tamez, Jimena Ramírez-Peris, Judith Pérez-Velázquez, Christina Kuttler, Ammar Jalalimanesh, Miguel Á. Saucedo-Mora, J. Guillermo Jiménez-Cortés, Toshinari Maeda, Yael González, María Tomás, Thomas K. Wood, Rodolfo García-Contreras
Frontiers in Microbiology.2018;[Epub] CrossRef

Mutation of the cyclic di-GMP phosphodiesterase gene in Burkholderia lata SK875 attenuates virulence and enhances biofilm formation: Hae-In Jung , Yun-Jung Kim , Yun-Jung Lee , Hee-Soo Lee , Jung-Kee Lee , Soo-Ki Kim; J. Microbiol. 2017;55(10):800-808. Published online September 28, 2017; DOI: https://doi.org/10.1007/s12275-017-7374-7

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Abstract

Burkholderia sp. is a gram-negative bacterium that commonly exists in the environment, and can cause diseases in plants, animals, and humans. Here, a transposon mutant library of a Burkholderia lata isolate from a pig with swine respiratory disease in Korea was screened for strains showing attenuated virulence in Caenorhabditis elegans. One such mutant was obtained, and the Tn5 insertion junction was mapped to rpfR, a gene encoding a cyclic di-GMP phosphodiesterase that functions as a receptor. Mutation of rpfR caused a reduction in growth on CPG agar and swimming motility as well as a rough colony morphology on Congo red agar. TLC analysis showed reduced AHL secretion, which was in agreement with the results from plate-based and bioluminescence assays. The mutant strain produced significantly more biofilm detected by crystal violet staining than the parent strain. SEM of the mutant strain clearly showed that the overproduced biofilm contained a filamentous structure. These results suggest that the cyclic di-GMP phosphodiesterase RpfR plays an important role in quorum sensing modulation of the bacterial virulence and biofilm formation.

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Biocontrol of bacterial wilt disease in tomato using Bacillus subtilis strain R31
Yunhao Sun, Yutong Su, Zhen Meng, Jie Zhang, Li Zheng, Shuang Miao, Di Qin, Yulan Ruan, Yanhui Wu, Lina Xiong, Xun Yan, Zhangyong Dong, Ping Cheng, Mingwei Shao, Guohui Yu
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D-Galactose as an autoinducer 2 inhibitor to control the biofilm formation of periodontopathogens: Eun-Ju Ryu , Jaehyun Sim , Jun Sim , Julian Lee , Bong-Kyu Choi; J. Microbiol. 2016;54(9):632-637. Published online August 31, 2016; DOI: https://doi.org/10.1007/s12275-016-6345-8

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Abstract

Autoinducer 2 (AI-2) is a quorum sensing molecule to which bacteria respond to regulate various phenotypes, including virulence and biofilm formation. AI-2 plays an important role in the formation of a subgingival biofilm composed mostly of Gram-negative anaerobes, by which periodontitis is initiated. The aim of this study was to evaluate D-galactose as an inhibitor of AI-2 activity and thus of the biofilm formation of periodontopathogens. In a search for an AI-2 receptor of Fusobacterium nucleatum, D-galactose binding protein (Gbp, Gene ID FN1165) showed high sequence similarity with the ribose binding protein (RbsB), a known AI-2 receptor of Aggregatibacter actinomycetemcomitans. D-Galactose was evaluated for its inhibitory effect on the AI-2 activity of Vibrio harveyi BB152 and F. nucleatum, the major coaggregation bridge organism, which connects early colonizing commensals and late pathogenic colonizers in dental biofilms. The inhibitory effect of D-galactose on the biofilm formation of periodontopathogens was assessed by crystal violet staining and confocal laser scanning microscopy in the absence or presence of AI-2 and secreted molecules of F. nucleatum. D-Galactose significantly inhibited the AI-2 activity of V. harveyi and F. nucleatum. In addition, D-galactose markedly inhibited the biofilm formation of F. nucleatum, Porphyromonas gingivalis, and Tannerella forsythia induced by the AI-2 of F. nucleatum without affecting bacterial growth. Our
results
demonstrate that the Gbp may function as an AI-2 receptor and that galactose may be used for prevention of the biofilm formation of periodontopathogens by targeting AI-2 activity.

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Research Support, Non-U.S. Gov'ts

D101 is critical for the function of AttJ, a repressor of quorum quenching system in Agrobacterium tumefaciens: Chao Wang , Chunlan Yan , Yong-Gui Gao , Lian-Hui Zhang; J. Microbiol. 2015;53(9):623-632. Published online August 1, 2015; DOI: https://doi.org/10.1007/s12275-015-5100-x

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Abstract: The quorum quenching system of Agrobacterium tumefaciens is specifically activated upon entering the stationary phase. Evidence has shown that this system includes two key components: the IclR-type transcriptional factor AttJ (also named as BlcR) and the AHL-lactonase AttM (also named as BlcC). At exponential phase, AttJ binds to the promoter region of attM and thus suppresses the expression of attM. At stationary phase, however, the small molecule SSA directly binds to AttJ and relieves its inhibition of AttJ and thereby triggers the expression of attM. While the regulation of AttM has been extensively investigated, little is known about the regulation of AttJ. In this study, we demonstrated the D101 amino acid of AttJ is essential for the AttJ function. In vitro, the variant protein of AttJD101H appeared to be readily aggregated. In vivo, the D101H mutation in AttJ entirely abolished the inhibitory activity of AttJ and overexpressed attM in A. tumefaciens A6. In addition, D101H mutation led to an overexpression of attJ, indicating an auto-regulatory mechanism for the attJ regulation. Put together, these findings demonstrate that D101 is an important amino acid for the transcription activity of AttJ and the transcription of attJ is regulated by a negative feedback loop. These results expand previous biochemical characterization of AttJ and provide new mechanistic insights into the regulation of quorum quenching in A. tumefaciens.

Note] Inhibition of quorum sensing in Pseudomonas aeruginosa by two herbal essential oils from Apiaceae family: Ehsan Sepahi , Saeed Tarighi , Farajollah Shahriari Ahmadi , Abdolreza Bagheri; J. Microbiol. 2015;53(2):176-180. Published online January 5, 2015; DOI: https://doi.org/10.1007/s12275-015-4203-8

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Abstract

Ferula (Ferula asafoetida L.) and Dorema (Dorema aucheri Bioss.) both from Apiaceae family were tested for their antiquorum sensing (QS) activity against Pseudomonas aeruginosa. Both essential oils exhibited anti-QS activity at 25 μg/ml of concenteration. At this concenteration Ferula fully abolished and Dorema reduced the violacein production by C. violaceum. Pyocyanin, pyoverdine, elastase and biofilm production were decreased in Ferula oil treatments. Dorema oil reduced pyoverdine and elastase production, while pyocyanin and biofilm production were not affacted. Expresion analysis of QS-dependent genes confirmed our phenotypic data. Our data introduced native Dorema and Ferula plants as novel QS and virulence inhibitors.

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Inhibitory Effects of 4-Hydroxy-2,5-Dimethyl-3(2H)-Furanone (HDMF) on Acyl-Homoserine Lactone-Mediated Virulence Factor Production and Biofilm Formation in Pseudomonas aeruginosa PAO1: Sung-Chan Choi , Can Zhang , Sooyoung Moon , Young-Sook Oh; J. Microbiol. 2014;52(9):734-742. Published online August 2, 2014; DOI: https://doi.org/10.1007/s12275-014-4060-x

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Abstract

4-Hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF), a nonhalogenated furanone found in a variety of fruits, has been shown to have antimicrobial activity. However, few studies have focused on its inhibitory effect on bacterial quorum sensing (QS) at levels below the non-inhibitory concentration. In this study, 0.1 μM HDMF decreased the production of QS signal molecules and inhibited QS-controlled biofilm formation by Pseudomonas aeruginosa PAO1 without causing growth inhibition. In the presence of 0.1 and 1.0 μM HDMF, biofilm production by PAO1 was reduced by 27.8 and 42.6%, respectively, compared to that by untreated control cells. HDMF (1.0 μM) also significantly affected virulence factor expression (regulated by the las, rhl, and pqs system), resulting in a significant reduction in the production of LasA protease (53.8%), rhamnolipid (40.9%), and pyocyanin (51.4%). This HDMF-dependent inhibition of virulence factor expression was overcome by increasing the levels of two QS signal molecules of P. aeruginosa, N-(3-oxo-dodecanoyl)-L-homoserine lactone and N-butyryl-L-homoserine lactone, suggesting reversible competitive inhibition between HDMF and these molecules. The results of this study indicate that HDMF has great potential as an inhibitor of QS, and that it may be of value as a therapeutic agent and in biofilm control, without increasing selective pressure for resistance development.

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A Functional and Phylogenetic Comparison of Quorum Sensing Related Genes in Brucella melitensis 16M: Aniel Jessica Leticia Brambila-Tapia , Ernesto Pérez-Rueda; J. Microbiol. 2014;52(8):709-715. Published online July 4, 2014; DOI: https://doi.org/10.1007/s12275-014-3570-x

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Abstract

A quorum-sensing (QS) system is involved in Brucella melitensis survival inside the host cell. Two transcriptional regulators identified in B. melitensis, BlxR and VjbR, regulate the expression of virB, an operon required for bacterial intracellular persistence. In this work, 628 genes affected by VjbR and 124 by BlxR were analyzed to gain insights into their functional and taxonomical distributions among the Bacteria and Archaea cellular domains. In this regard, the Cluster of Orthologous Groups (COG) genes and orthologous genes in 789 nonredundant bacterial and archaeal genomes were obtained and compared against a group of randomly selected genes. From these analyses, we found 71 coaffected genes between VjbR and BlxR. In the COG comparison, VjbR activated genes associated with intracellular trafficking, secretion and vesicular transport and defense mechanisms, while BlxR affected genes related to energy production and conversion (with an equal effect) and translation, ribosomal structure and biogenesis, posttranslational modifications and carbohydrate and amino acid metabolism (with a negative effect). When the taxonomical distribution of orthologous genes was evaluated, the VjbR- and BlxRrelated genes presented more orthologous genes in Crenarchaeota (Archaea), Firmicutes, and Tenericutes and fewer genes in Proteobacteria than expected by chance. These findings suggest that QS system exert a fine-tuning modulation of gene expression, by which VjbR activates genes related to infection persistence and defense, while BlxR represses general bacterial metabolism for intracellular adaptations. Finally, these affected genes present a degree of presence among Bacteria and Archaea genomes that is different from that expected by chance.

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Brucella mediates autophagy, inflammation, and apoptosis to escape host killing
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Review

REVIEW] Mechanisms of Synergy in Polymicrobial Infections: Justine L. Murray , Jodi L. Connell , Apollo Stacy , Keith H. Turner , Marvin Whiteley; J. Microbiol. 2014;52(3):188-199. Published online March 1, 2014; DOI: https://doi.org/10.1007/s12275-014-4067-3

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Abstract

Communities of microbes can live almost anywhere and contain many different species. Interactions between members of these communities often determine the state of the habitat in which they live. When these habitats include sites on the human body, these interactions can affect health and disease. Polymicrobial synergy can occur during infection, in which the combined effect of two or more microbes on disease is worse than seen with any of the individuals alone. Powerful genomic methods are increasingly used to study microbial communities, including metagenomics to reveal the members and genetic content of a community and metatranscriptomics to describe the activities of community members. Recent efforts focused toward a mechanistic understanding of these interactions have led to a better appreciation of the precise bases of polymicrobial synergy in communities containing bacteria, eukaryotic microbes, and/or viruses. These studies have benefited from advances in the development of in vivo models of polymicrobial infection and modern techniques to profile the spatial and chemical bases of intermicrobial communication. This review describes the breadth of mechanisms microbes use to interact in ways that impact pathogenesis and techniques to study polymicrobial communities.

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Mass spectrometric metabolomic imaging of biofilms on corroding steel surfaces using laser ablation and solvent capture by aspiration
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The Upper Respiratory Tract as a Microbial Source for Pulmonary Infections in Cystic Fibrosis. Parallels from Island Biogeography
Katrine L. Whiteson, Barbara Bailey, Megan Bergkessel, Douglas Conrad, Laurence Delhaes, Ben Felts, J. Kirk Harris, Ryan Hunter, Yan Wei Lim, Heather Maughan, Robert Quinn, Peter Salamon, James Sullivan, Brandie D. Wagner, Paul B. Rainey
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Reducing the bioactivity of Tannerella forsythia lipopolysaccharide by Porphyromonas gingivalis
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