Journal of Microbiology

Journal Article

Isolation of Synthetic Lethal Mutations in the rsm1-null Mutant of Fission Yeast: DongGeRaMi Moon , Yun-Sun Park , Cha-Yeon Kim , Jin Ho Yoon; J. Microbiol. 2010;48(5):701-705. Published online November 3, 2010; DOI: https://doi.org/10.1007/s12275-010-0353-x

Abstract: To identify mutations in genes that are genetically linked to rsm1, we performed a synthetic lethal genetic screen in the fission yeast, Schizosaccharomyces pombe. Four mutations that showed synthetic lethality in combination with the rsm1null allele were isolated from approximately 320,000 colonies and defined in three complementation groups. One mutant (SLrsm1) exhibited a significant accumulation of poly(A)+ RNA in the nucleus under synthetic lethal conditions, while the rest had no mRNA export defects. In addition, some genes (spmex67, rae1, or mlo3) required for mRNA export complemented the growth defects of the identified mutants. These results suggest that the isolated mutants contain mutations in genes that are involved in mRNA export and/or pre-mRNA retention.

Subcellular Localization of Catalase Encoded by the ctt1^+ Gene in Schizosaccharomyces pombe: Sang-il Lee , Joon Lee , Jung-Hye Roe; J. Microbiol. 2000;38(3):156-159.

Abstract: The ctt1^+ gene in Schizosaccharomyces pombe encodes a catalase responsible for H_2O_2 -resistance of this organism as judged by the H_2O_2 -sensitive phenotype of the ctt1[delta] mutant. In this study, we investigated the subcellular localization of the Ctt1 gene product. In wild type cells catalase activity was detected in the organelle fraction as well as in the cytosol. The ctt1[delta] mutant contained no catalase activity, indicating that both cytosolic and organellar catalases are the products of a single ctt1^+ gene. Western blot analysis revealed two catalase bands, both of which disappeared in the ctt1[delta] mutant. The major, faster-migrating band existed in the cytosol whereas the minor, slower-migrating band appeared to be located in organelles, most likely in peroxisomes. These results suggest that the ctt1^++ gene product targeted to the peroxisome is a modified form of the one in the cytosol.

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