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Isolation of Synthetic Lethal Mutations in the rsm1-null Mutant of Fission Yeast
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Journal Article
Isolation of Synthetic Lethal Mutations in the rsm1-null Mutant of Fission Yeast
DongGeRaMi Moon 1, Yun-Sun Park 2, Cha-Yeon Kim 2, Jin Ho Yoon 1
Journal of Microbiology 2010;48(5):701-705
DOI: https://doi.org/10.1007/s12275-010-0353-x
Published online: November 3, 2010
1School of Biological Sciences and Chemistry, 2Department of Education, and Basic Sciences Research Institute, Sungshin Women's University, Seoul 136-742, Republic of Korea1School of Biological Sciences and Chemistry, 2Department of Education, and Basic Sciences Research Institute, Sungshin Women's University, Seoul 136-742, Republic of Korea
Corresponding author:  Jin Ho Yoon , Tel: +82-2-920-7675, 
Received: 6 September 2010   • Accepted: 27 September 2010
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Abstract

To identify mutations in genes that are genetically linked to rsm1, we performed a synthetic lethal genetic screen in the fission yeast, Schizosaccharomyces pombe. Four mutations that showed synthetic lethality in combination with the rsm1null allele were isolated from approximately 320,000 colonies and defined in three complementation groups. One mutant (SLrsm1) exhibited a significant accumulation of poly(A)+ RNA in the nucleus under synthetic lethal conditions, while the rest had no mRNA export defects. In addition, some genes (spmex67, rae1, or mlo3) required for mRNA export complemented the growth defects of the identified mutants. These results suggest that the isolated mutants contain mutations in genes that are involved in mRNA export and/or pre-mRNA retention.

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    Isolation of Synthetic Lethal Mutations in the rsm1-null Mutant of Fission Yeast
    J. Microbiol. 2010;48(5):701-705.   Published online November 3, 2010
    DOI: https://doi.org/10.1007/s12275-010-0353-x
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