Isolation of Synthetic Lethal Mutations in the rsm1-null Mutant of Fission Yeast

DongGeRaMi Moon; Yun-Sun Park; Cha-Yeon Kim; Jin Ho Yoon

doi:10.1007/s12275-010-0353-x

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Isolation of Synthetic Lethal Mutations in the rsm1-null Mutant of Fission Yeast: DongGeRaMi Moon ¹, Yun-Sun Park ², Cha-Yeon Kim ², Jin Ho Yoon ¹; Journal of Microbiology 2010;48(5):701-705.
DOI: https://doi.org/10.1007/s12275-010-0353-x
Published online: November 3, 2010

¹School of Biological Sciences and Chemistry, ²Department of Education, and Basic Sciences Research Institute, Sungshin Women's University, Seoul 136-742, Republic of Korea¹School of Biological Sciences and Chemistry, ²Department of Education, and Basic Sciences Research Institute, Sungshin Women's University, Seoul 136-742, Republic of Korea

Corresponding author: Jin Ho Yoon , Tel: +82-2-920-7675,

Received: 6 September 2010 • Accepted: 27 September 2010

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Abstract

To identify mutations in genes that are genetically linked to rsm1, we performed a synthetic lethal genetic screen in the fission yeast, Schizosaccharomyces pombe. Four mutations that showed synthetic lethality in combination with the rsm1null allele were isolated from approximately 320,000 colonies and defined in three complementation groups. One mutant (SLrsm1) exhibited a significant accumulation of poly(A)+ RNA in the nucleus under synthetic lethal conditions, while the rest had no mRNA export defects. In addition, some genes (spmex67, rae1, or mlo3) required for mRNA export complemented the growth defects of the identified mutants. These results suggest that the isolated mutants contain mutations in genes that are involved in mRNA export and/or pre-mRNA retention.

Keywords: rsm1;synthetic lethality;mRNA export;S. pombe

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Scientific Reports.2022;[Epub] CrossRef
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The Korean Journal of Microbiology.2014; 50(3): 249. CrossRef

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Isolation of Synthetic Lethal Mutations in the rsm1-null Mutant of Fission Yeast

J. Microbiol. 2010;48(5):701-705. Published online November 3, 2010

DOI: https://doi.org/10.1007/s12275-010-0353-x

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