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- Development of a Method Based on Surface Enhanced Laser Desorption and Ionization Time of Flight Mass Spectrometry for Rapid Identification of Klebsiella pneumoniae
- Daiwen Xiao , Yongchang Yang , Hua Liu , Hua Yu , Yingjun Yan , Wenfang Huang , Wei Jiang , Weijin Liao , Qi Hu , Bo Huang
- J. Microbiol. 2009;47(5):646-650. Published online October 24, 2009
- DOI: https://doi.org/10.1007/s12275-009-0092-z
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Abstract
- A method based on surface enhanced laser desorption and ionization time of flight mass spectrometry (SELDI-TOF MS) was developed for the rapid identification of Klebsiella pneumoniae by directly applying bacterial colonies without further protein extraction. A total of 40 K. pneumoniae and 114 other related microorganisms isolated clinically were analyzed by SELDI-TOF MS. An identification model for K. pneumoniae was established by artificial neural networks (ANNs) with classification accuracy of 100%. The model was blindly tested with 43 K. pneumoniae and 53 control bacteria again. The results showed that the model was successful with accuracy of 96.9%, sensitivity of 100% and specificity of 94.3%. This strategy is potential for rapid identification of K. pneumoniae.
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- Direct common gram-negative bacterial identification from positive blood culture bottles by SELDI-TOF MS
Daiwen Xiao, Yongchang Yang, Wei Jiang, Hangfeng Zhang, Hua Liu, Hua Yu, Chunbao Xie, Min Zhong, Liang Chen, Wenfang Huang
Journal of Microbiological Methods.2014; 105: 116. CrossRef - Advances in mass spectrometry for the identification of pathogens
YenâPeng Ho, P. Muralidhar Reddy
Mass Spectrometry Reviews.2011; 30(6): 1203. CrossRef
- Direct common gram-negative bacterial identification from positive blood culture bottles by SELDI-TOF MS
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