Journal of Microbiology

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Antiviral effects of heme oxygenase-1 against canine coronavirus and canine influenza virus in vitro: Jae-Hyeong Kim, Dong-Hwi Kim, Kyu-Beom Lim, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Dong-Hun Lee, Do-Geun Kim, Hun-Young Yoon, In-Soo Choi; J. Microbiol. 2025;63(5):e2501029. Published online May 27, 2025; DOI: https://doi.org/10.71150/jm.2501029

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Abstract PDF Supplementary Material: Heme oxygenase-1 (HO-1) has antioxidant, anti-apoptotic, and anti-inflammatory properties. Emerging evidence shows that HO-1 also exhibits antiviral activity against severe acute respiratory syndrome coronavirus 2, human immunodeficiency virus, hepatitis B virus, and Ebola virus. Its antiviral effects are mediated not only by its enzymatic function but also through the modulation of interferon-related pathways, thereby inhibiting viral replication. In this study, we investigated the antiviral effects of HO-1 on canine coronavirus (CCoV) and canine influenza virus (CIV) H3N2 using cell-based assays. To determine whether HO-1 suppresses CCoV and CIV, cells were treated with hemin to induce HO-1 expression. Hemin treatment successfully induced HO-1 expression in A72 and Madin-Darby canine kidney cells, resulting in the suppression of CCoV and CIV replication. The canine HO-1 gene was cloned into an expression vector and transfected into cells to achieve transient overexpression. Recombinant canine HO-1 protein was expressed in Escherichia coli and purified using an expression vector. HO-1 overexpression suppressed CCoV and CIV replication in cells. Following viral infection, treatment with purified HO-1 protein led to a reduction in viral protein levels. Therefore, both HO-1 expression and exogenous protein treatment effectively inhibited CCoV and CIV replication. Elevated HO-1 protein levels consistently reduced viral RNA and protein expression in vitro. These findings suggest that HO-1 could serve as a potential therapeutic agent for managing viral infections in dogs.

Research Support, Non-U.S. Gov't

Development of a Chimeric Strain of Porcine Reproductive and Respiratory Syndrome Virus with an Infectious Clone and a Korean Dominant Field Strain: Jung-Ah Lee , Nak-Hyung Lee , Sang-Won Lee , Seung-Yong Park , Chang-Seon Song , In-Soo Choi , Joong-Bok Lee; J. Microbiol. 2014;52(4):345-349. Published online March 29, 2014; DOI: https://doi.org/10.1007/s12275-014-4074-4

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The K418 chimeric virus of porcine reproductive and respiratory syndrome virus (PRRSV) was engineered by replacing the genomic region containing structure protein genes of an infectious clone of PRRSV, FL12, with the same region obtained from a Korean dominant field strain, LMY. The K418 reached 106 TCID50/ml of viral titer with similar growth kinetics to those of parental strains and had a cross-reactive neutralizing antibody response to field serum from the entire country. The chimeric clone pK418 can be used as a practical tool for further studying the molecular characteristics of PRRSV proteins through genetic manipulation. Furthermore, successful construction of the K418 will allow for the development of customized vaccine candidates against PRRSV, which has evolved rapidly in Korea.

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Vaccines.2021; 9(11): 1258. CrossRef
Comparative Evaluation of Antibacterial Activity of Probiotics SK12 and SM18: An In Vitro Study
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International Journal of Clinical Pediatric Dentistry.2021; 13(6): 611. CrossRef
A chimeric porcine reproductive and respiratory syndrome virus (PRRSV)-2 vaccine is safe under international guidelines and effective both in experimental and field conditions
Hwi-Yeon Choi, So-Hyun Lee, So-Hyeun Ahn, Jong-Chul Choi, Ji-Yun Jeong, Beom-Joo Lee, Yeong-Lim Kang, Seong-Soo Hwang, Jung-Keun Lee, Sang-Won Lee, Seung-Yong Park, Chang-Seon Song, In-Soo Choi, Joong-Bok Lee
Research in Veterinary Science.2021; 135: 143. CrossRef
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Irfan Yaqoob Wani, Khushpreet Singh
Materials Today: Proceedings.2020; 33: 1706. CrossRef
In vitro and in vivo studies of deglycosylated chimeric porcine reproductive and respiratory syndrome virus as a vaccine candidate and its realistic revenue impact at commercial pig production level
Jung-ju Kim, Jung-Ah Lee, Hwi-yeon Choi, Jang-hyuck Han, Won Huh, Jae-Ho Pi, Jung-Keun Lee, Sangshin Park, Ki-hyun Cho, Joong-bok Lee
Vaccine.2017; 35(37): 4966. CrossRef
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Nadeem Shabir, Amina Khatun, Salik Nazki, Bumseok Kim, Eun-Jin Choi, Dong Sun, Kyoung-Jin Yoon, Won-Il Kim
Viruses.2016; 8(8): 240. CrossRef
Augmented immune responses in pigs immunized with an inactivated porcine reproductive and respiratory syndrome virus containing the deglycosylated glycoprotein 5 under field conditions
Jung-Ah Lee, Nak-Hyung Lee, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, In-Soo Choi, Sang-Won Lee
Clinical and Experimental Vaccine Research.2016; 5(1): 70. CrossRef
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Yamile Valencia González, José Carvalho-Camapum, Luis Augusto Lara-Valencia
DYNA.2015; 82(192): 221. CrossRef
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Paulo J. Venda Oliveira, Milton S. da Costa, João N. P. Costa, M. Fernanda Nobre
Journal of Materials in Civil Engineering.2015;[Epub] CrossRef
Live porcine reproductive and respiratory syndrome virus vaccines: Current status and future direction
Gourapura J. Renukaradhya, Xiang-Jin Meng, Jay G. Calvert, Michael Roof, Kelly M. Lager
Vaccine.2015; 33(33): 4069. CrossRef
Influence of biomineralization on the physico-mechanical profile of a tropical soil affected by erosive processes
Y. Valencia, J. Camapum, F. Araripe Torres
Soil Biology and Biochemistry.2014; 74: 98. CrossRef
Influences on the carbonate hydrochemistry of mound spring environments, Lake Eyre South region, South Australia
Mark N. Keppel, Vincent E.A. Post, Andrew J. Love, Jonathan D.A. Clarke, Adrian D. Werner
Chemical Geology.2012; 296-297: 50. CrossRef
Mound springs in the arid Lake Eyre South region of South Australia: A new depositional tufa model and its controls
Mark N. Keppel, Jonathan D.A. Clarke, Todd Halihan, Andrew J. Love, Adrian D. Werner
Sedimentary Geology.2011; 240(3-4): 55. CrossRef
Effect of ureolytic bacteria on concrete properties
Rafat Siddique, Navneet Kaur Chahal
Construction and Building Materials.2011; 25(10): 3791. CrossRef

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