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Development of a Chimeric Strain of Porcine Reproductive and Respiratory Syndrome Virus with an Infectious Clone and a Korean Dominant Field Strain
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Research Support, Non-U.S. Gov't
Development of a Chimeric Strain of Porcine Reproductive and Respiratory Syndrome Virus with an Infectious Clone and a Korean Dominant Field Strain
Jung-Ah Lee , Nak-Hyung Lee , Sang-Won Lee , Seung-Yong Park , Chang-Seon Song , In-Soo Choi , Joong-Bok Lee
Journal of Microbiology 2014;52(4):345-349
DOI: https://doi.org/10.1007/s12275-014-4074-4
Published online: March 29, 2014
Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of KoreaLaboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea
Corresponding author:  Joong-Bok Lee , Tel: +82-2-450-3714, 
Received: 5 February 2014   • Revised: 11 March 2014   • Accepted: 13 March 2014
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Abstract

The K418 chimeric virus of porcine reproductive and respiratory syndrome virus (PRRSV) was engineered by replacing the genomic region containing structure protein genes of an infectious clone of PRRSV, FL12, with the same region obtained from a Korean dominant field strain, LMY. The K418 reached 106 TCID50/ml of viral titer with similar growth kinetics to those of parental strains and had a cross-reactive neutralizing antibody response to field serum from the entire country. The chimeric clone pK418 can be used as a practical tool for further studying the molecular characteristics of PRRSV proteins through genetic manipulation. Furthermore, successful construction of the K418 will allow for the development of customized vaccine candidates against PRRSV, which has evolved rapidly in Korea.

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    Development of a Chimeric Strain of Porcine Reproductive and Respiratory Syndrome Virus with an Infectious Clone and a Korean Dominant Field Strain
    J. Microbiol. 2014;52(4):345-349.   Published online March 29, 2014
    DOI: https://doi.org/10.1007/s12275-014-4074-4
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