Journal Article
	
	
				- The synthetic human beta-defensin-3 C15 peptide exhibits antimicrobial activity against Streptococcus mutans, both alone and in combination with dental disinfectants
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		Ki Bum Ahn , A Reum Kim , Kee-Yeon Kum , Cheol-Heui Yun , Seung Hyun Han 		
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			J. Microbiol. 2017;55(10):830-836.   Published online September 28, 2017		
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							DOI: https://doi.org/10.1007/s12275-017-7362-y
					
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						 Abstract Abstract PDF PDF
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		Streptococcus mutans is a major etiologic agent of human
dental caries that forms biofilms on hard tissues in the human
oral cavity, such as tooth and dentinal surfaces. Human
β-defensin-3 (HBD3) is a 45-amino-acid natural antimicrobial
peptide that has broad spectrum antimicrobial activity
against bacteria and fungi. A synthetic peptide consisting of
the C-terminal 15 amino acids of HBD3 (HBD3-C15) was
recently shown to be sufficient for its antimicrobial activity.
Thus, clinical applications of this peptide have garnered
attention. In this study, we investigated whether HBD3-C15
inhibits the growth of the representative cariogenic pathogen
Streptococcus mutans and its biofilm formation. HBD3-C15
inhibited bacterial growth, exhibited bactericidal activity,
and attenuated bacterial biofilm formation in a dose-dependent
manner. HBD3-C15 potentiated the bactericidal and
anti-biofilm activity of calcium hydroxide (CH) and chlorhexidine
digluconate (CHX), which are representative disinfectants
used in dental clinics, against S. mutans. Moreover,
HBD3-C15 showed antimicrobial activity by inhibiting biofilm
formation by S. mutans and other dentinophilic bacteria
such as Enterococcus faecalis and Streptococcus gordonii,
which are associated with dental caries and endodontic
infection, on human dentin slices. These effects were observed
for HBD3-C15 alone and for HBD3-C15 in combination with
CH or CHX. Therefore, we suggest that HBD3-C15 is a potential
alternative or additive disinfectant that can be used
for the treatment of oral infectious diseases, including dental
caries and endodontic infections.	
		
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 Research Support, Non-U.S. Gov'ts
	
	
				- Inhibitory Effect of Lactobacillus reuteri on Periodontopathic and Cariogenic Bacteria
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		Mi-Sun Kang , Jong-Suk Oh , Hyun-Chul Lee , Hoi-Soon Lim , Seok-Woo Lee , Kyu-Ho Yang , Nam-Ki Choi , Seon-Mi Kim 		
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			J. Microbiol. 2011;49(2):193-199.   Published online May 3, 2011		
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							DOI: https://doi.org/10.1007/s12275-011-0252-9
					
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						 Abstract Abstract PDF PDF
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		The interaction between Lactobacillus reuteri, a probiotic bacterium, and oral pathogenic bacteria have not been studied adequately. This study examined the effects of L. reuteri on the proliferation of periodontopathic bacteria including Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, and Tannerella forsythia, and on the formation of Streptococcus mutans biofilms. Human-derived L. reuteri strains (KCTC 3594 and KCTC 3678) and rat-derived L. reuteri KCTC 3679 were used. All strains exhibited significant inhibitory effects on the growth of periodontopathic bacteria and the formation of S. mutans
biofilms. These antibacterial activities of L. reuteri were attributed to the production of organic acids, hydrogen peroxide, and a bacteriocin-like compound. Reuterin, an antimicrobial factor, was produced only by L. reuteri KCTC 3594. In addition, L. reuteri inhibited the production of methyl mercaptan by F. nucleatum and P. gingivalis. Overall, these results suggest that L. reuteri may be useful as a probiotic agent for improving oral health.	
		
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				- Antibacterial Characteristics of Curcuma xanthorrhiza Extract on Streptococcus mutans Biofilm
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		Jung-Eun Kim , Hee-Eun Kim , Jae-Kwan Hwang , Ho-Jeong Lee , Ho-Keun Kwon , Baek-Il Kim 		
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			J. Microbiol. 2008;46(2):228-232.   Published online June 11, 2008		
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							DOI: https://doi.org/10.1007/s12275-007-0167-7
					
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						 Abstract Abstract PDF PDF
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		This study evaluated the antibacterial effects of a natural Curcuma xanthorrhiza extract (Xan) on a Streptococcus mutans biofilm by examining the bactericidal activity, inhibition of acidogenesis and morphological alteration. Xan was obtained from the roots of a medicinal plant in Indonesia, which has shown selective
<br><br>antibacterial effects on planktonic S. mutans. S. mutans biofilms were formed on slide glass over a 72 h period and treated with the following compounds for 5, 30, and 60 min: saline, 1% DMSO, 2 mg/ml chlorhexidine (CHX), and 0.1 mg/ml Xan. The Xan group exposed for 5 and 30 min showed significantly fewer colony forming units (CFU, 57.6 and 97.3%, respectively) than those exposed to 1% DMSO, the negative control group (P<0.05). These CFU were similar in number to those slides exposed to CHX, the positive control group. Xan showed similar bactericidal effect to that of CHX but the dose of Xan was one twentieth that of CHX. In addition, the biofilms treated with Xan and CHX maintained a neutral pH for 4 h, which indicates that Xan and CHX inhibit acid production. Scanning electron microscopy showed morphological changes in the cell wall and membrane of the Xan-treated biofilms; an uneven surface and a deformation in contour. Overall, natural Xan has strong bactericidal activity, inhibitory effects on acidogenesis, and alters the microstructure of S. mutans biofilm. In conclusion, Xan has potential in anti-S. mutans therapy for the prevention of dental caries.	
		
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				- Effect of Leuconostoc spp. on the Formation of Streptococcus mutans Biofilm
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		Mi-Sun Kang , In-Chol Kang , Seon-Mi Kim , Hyun-Chul Lee , Jong-Suk Oh 		
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			J. Microbiol. 2007;45(4):291-296. 		
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							DOI: https://doi.org/2570 [pii]
					
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						 Abstract Abstract PDF PDF
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		Insoluble glucans synthesized by Streptococcus mutans enhance the pathogenicity of oral biofilm by promoting the adherence and accumulation of cariogenic bacteria on the surface of the tooth. The objective of this study was to investigate the effect of Leuconostoc spp. on the in vitro formation of S. mutans biofilm. Three strains, Leuconostoc gelidum ATCC 49366, Leuconostoc mesenteroides ssp. cremoris ATCC 19254 and Leuconostoc mesenteroides ssp. mesenteroides ATCC 8293, were used in this study. They exhibited profound inhibitory effects on the formation of S. mutans biofilm and on the proliferation of S. mutans. The water-soluble polymers produced from sucrose were most strongly produced by L. gelidum, followed by L. mesenteroides ssp. cremoris and L. mesenteroides ssp. mesenteroides. The mean wet weights of the artificial biofilm of S. mutans were also significantly reduced as a result of the addition of the water-soluble polymers obtained from Leuconostoc cultures. According to the results of thin-layer chromatographic analysis, the hydrolysates of the water-soluble polymers produced by Leuconostoc were identical to those of dextran T-2000, forming predominately α-(1-6) glucose linkages. These results indicate that dextran-producing Leuconostoc strains are able to inhibit the formation of S. mutans biofilm in vitro.