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- Salmonella Typhimurium ST313 isolated in Brazil revealed to be more invasive and inflammatory in murine colon compared to ST19 strains
- Amanda Aparecida Seribelli , Tamara R. Machado Ribeiro , Patrick da Silva† , Isabela Mancini Martins , Felipe Pinheiro Vilela , Marta I. Cazentini Medeiros , Kamila Chagas Peronni , Wilson Araújo da Silva Junior , Cristiano Gallina Moreira , Juliana Pfrimer Falcão
- J. Microbiol. 2021;59(9):861-870. Published online August 12, 2021
- DOI: https://doi.org/10.1007/s12275-021-1082-z
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Abstract
- Salmonella Typhimurium (ST313) has caused an epidemic of invasive disease in sub-Saharan Africa and has been recently identified in Brazil. As the virulence of this ST is poorly understood, the present study aimed to (i) perform the RNAseq in vitro of S. Typhimurium STm30 (ST313) grown in Luria-Bertani medium at 37°C; (ii) compare it with the RNAseq of the S. Typhimurium SL1344 (ST19) and S. Typhimurium STm11 (ST19) strains under the same growing conditions; and (iii) examine the colonization capacity and expression of virulence genes and cytokines in murine colon. The STm30 (ST313) strain exhibited stronger virulence and was associated with a more inflammatory profile than the strains SL1344 (ST19) and STm11 (ST19), as demonstrated by transcriptome and in vivo assay. The expression levels of the hilA, sopD2, pipB, and ssaS virulence genes, other Salmonella pathogenicity islands SPI-1 and SPI-2 genes or effectors, and genes of the cytokines IL-1β, IFN-γ, TNF-α, IL-6, IL-17, IL-22, and IL-12 were increased during ST313 infection in C57BL/6J mice. In conclusion, S. Typhimurium STm30 (ST313) isolated from human feces in Brazil express higher levels of pathogenesis- related genes at 37°C and has stronger colonization and invasion capacity in murine colon due to its high expression levels of virulence genes, when compared with the S. Typhimurium SL1344 (ST19) and STm11 (ST19) strains. STm30 (ST313) also induces stronger expression of pro-inflammatory cytokines in this organ, suggesting that it causes more extensive tissue damage.
- Performance comparison of fecal preservative and stock solutions for gut microbiome storage at room temperature
- Chanhyeok Park , Kyeong Eui Yun , Jeong Min Chu , Ji Yeon Lee , Chang Pyo Hong , Young Do Nam , Jinuk Jeong , Kyudong Han , Yong Ju Ahn
- J. Microbiol. 2020;58(8):703-710. Published online June 25, 2020
- DOI: https://doi.org/10.1007/s12275-020-0092-6
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Abstract
- The gut microbiome, which is symbiotic within the human
body, assists in human digestion. It plays significant roles
in identifying intestinal disease as well as in maintaining a
healthy body with functional immune and metabolic activities.
To confirm the consistency of fecal intestinal microbial
research, it is necessary to study the changes in intestinal microbial
flora according to the fecal collection solution and
storage period. We collected fecal samples from three healthy
Korean adults. To examine the efficacy of fecal collection solution,
we used NBgene-Gut, OMNIgene-Gut, 70% ethanol
(Ethanol-70%), and RNAlater. The samples were stored for
up to two months at room temperature using three different
methods
, and we observed changes in microbial communities over time. We analyzed clusters of changes in the microbial flora by observing fecal stock solutions and metagenome sequencing performed over time. In particular, we confirmed the profiling of alpha and beta diversity and microbial classification according to the differences in intestinal environment among individuals. We also confirmed that the microbial profile remained stable for two months and that the microbial profile did not change significantly over time. In addition, our results suggest the possibility of verifying microbial profiling even for long-term storage of a single sample. In conclusion, collecting fecal samples using a stock solution rather than freezing feces seems to be relatively reproducible and stable for GUT metagenome analysis. Therefore, stock solution tubes in intestinal microbial research can be used without problems.
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