Review
- Role of Rab GTPases in Bacteria Escaping from Vesicle Trafficking of Host Cells
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Huiling Xu, Shengnan Wang, Xiaozhou Wang, Pu Zhang, Qi Zheng, ChangXi Qi, Xiaoting Liu, Muzi Li, Yongxia Liu, Jianzhu Liu
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J. Microbiol. 2024;62(8):581-590. Published online August 30, 2024
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DOI: https://doi.org/10.1007/s12275-024-00162-9
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262
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Most bacteria will use their toxins to interact with the host cell, causing damage to the cell and then escaping from it. When bacteria enter the cell, they will be transported via the endosomal pathway. Rab GTPases are involved in bacterial transport as major components of endosomes that bind to their downstream effector proteins. The bacteria manipulate some Rab GTPases, escape the cell, and get to survive. In this review, we will focus on summarizing the many processes of how bacteria manipulate Rab GTPases to control their escape.
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- Rab5-mediated phagocytosis restricts Spiroplasma eriocheiris infection in crabs through a ubiquitination-dependent mechanism
Yubo Ma, Yu Yao, Xin Yin, Zhenyu Yu, Jing Yan, Yaqin Wang, Wei Gu, Xuguang Li, Jun Zhou, Qingguo Meng
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Journal Article
- Cyanobacterial biodiversity of semiarid public drinking water supply reservoirs assessed via next-generation DNA sequencing technology
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Adriana Sturion Lorenzi , Mathias Ahii Chia , Fabyano Alvares Cardoso Lopes , Genivaldo Gueiros Z. Silva , Robert A. Edwards , Maria do Carmo Bittencourt-Oliveira
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J. Microbiol. 2019;57(6):450-460. Published online May 27, 2019
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DOI: https://doi.org/10.1007/s12275-019-8349-7
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222
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Next-generation DNA sequencing technology was applied
to generate molecular data from semiarid reservoirs during
well-defined seasons. Target sequences of 16S-23S rRNA ITS
and cpcBA-IGS were used to reveal the taxonomic groups of
cyanobacteria present in the samples, and genes coding for
cyanotoxins such as microcystins (mcyE), saxitoxins (sxtA),
and cylindrospermopsins (cyrJ) were investigated. The presence
of saxitoxins in the environmental samples was evaluated
using ELISA kit. Taxonomic analyses of high-throughput
DNA sequencing data showed the dominance of the genus
Microcystis in Mundaú reservoir. Furthermore, it was the
most abundant genus in the dry season in Ingazeira reservoir.
In the rainy season, 16S-23S rRNA ITS analysis revealed that
Cylindrospermopsis raciborskii comprised 46.8% of the cyanobacterial
community in Ingazeira reservoir, while the cpcBAIGS
region revealed that C. raciborskii (31.8%) was the most
abundant taxon followed by Sphaerospermopsis aphanizomenoides
(17.3%) and Planktothrix zahidii (16.6%). Despite
the presence of other potential toxin-producing genera, the
detected sxtA gene belonged to C. raciborskii, while the mcyE
gene belonged to Microcystis in both reservoirs. The detected
mcyE gene had good correlation with MC content, while the
amplification of the sxtA gene was related to the presence of
STX. The cyrJ gene was not detected in these samples. Using
DNA analyses, our results showed that the cyanobacterial
composition of Mundaú reservoir was similar in successive
dry seasons, and it varied between seasons in Ingazeira reservoir.
In addition, our data suggest that some biases of analysis
influenced the cyanobacterial communities seen in
the NGS output of Ingazeira reservoir.
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Medina O Kadiri, Solomon Isagba, Jeffrey U Ogbebor, Osasere A. Omoruyi, Timothy E. Unusiotame-Owolagba, Adriana Sturion Lorenzi, Maria do Carmo Bittencourt-Oliveira, Mathias Ahii Chia
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Review
- MINIREVIEW] The Potential Hazards of Aspergillus sp. in Foods and Feeds, and the Role of Biological Treatment: A Review
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Sheikh Imranudin Sheikh-Ali , Akil Ahmad , Siti-Hamidah Mohd-Setapar , Zainul Akmal Zakaria , Norfahana Abdul-Talib , Aidee Kamal Khamis , Md Enamul Hoque
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J. Microbiol. 2014;52(10):807-818. Published online October 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-4294-7
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The contamination of food and feed by Aspergillus has become
a global issue with a significant worldwide economic
impact. The growth of Aspergillus is unfavourable to the
development of food and feed industries, where the problems
happen mostly due to the presence of mycotoxins, which is a
toxic metabolite secreted by most Aspergillus groups. Moreover,
fungi can produce spores that cause diseases, such as
allergies and asthma, especially to human beings. High temperature,
high moisture, retarded crops, and poor food storage
conditions encourage the growth of mold, as well as
the development of mycotoxins. A variety of chemical, biological,
and physical strategies have been developed to control
the production of mycotoxins. A biological approach,
using a mixed culture comprised of Saccharomyces cerevisiae
and Lactobacillus rhamnosus resulted in the inhibition of the
growth of fungi when inoculated into fermented food. The
results
reveal that the mixed culture has a higher potential
(37.08%) to inhibit the growth of Aspergillus flavus (producer
of Aflatoxin) compared to either single culture, L. rhamnosus
NRRL B-442 and S. cerevisiae, which inhibit the growth by
63.07% and 64.24%, respectively.
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Research Support, Non-U.S. Gov't
- Molecular Characterization of Atoxigenic Aspergillus flavus Isolates Collected in China
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Dandan Wei , Lu Zhou , Jonathan Nimal Selvaraj , Chushu Zhang , Fuguo Xing , Yueju Zhao , Yan Wang , Yang Liu
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J. Microbiol. 2014;52(7):559-565. Published online May 30, 2014
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DOI: https://doi.org/10.1007/s12275-014-3629-8
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228
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Aspergillus flavus strains were isolated from peanut fields of Liaoning, Shandong, Hubei and Guangdong Provinces in China, and identified through phenotypic and molecular approaches. Of the 323 A. flavus strains isolated, 76 strains did not produce aflatoxins detectable by UPLC. The incidence of atoxigenic A. flavus strains decreased with increase in temperature and increased with increase in latitude in different geographical locations. Amplification of all the aflatoxin genes in the aflatoxin gene cluster in the atoxigenic isolates showed that there were 25 deletion patterns (A-Y), with 22 deletion patterns identified for the first time. Most of the atoxigenic A. flavus isolates with gene deletions (97%) had deletions in at least one of the four genes (aflT, nor-1, aflR, and hypB), indicating that these four genes could be targeted for rapid identification of atoxigenic strains. The atoxigenic isolates with gene deletions, especially the isolates with large deletions, are potential candidates for aflatoxin control.
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Journal Article
- Comparative Analysis of Superantigen Genes in Staphylococcus xylosus and Staphylococcus aureus Isolates Collected from a Single Mammary Quarter of Cows with Mastitis
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Karol Fijałkowski , Magdalena Struk , Jolanta Karakulska , Aleksandra Paszkowska , Stefania Giedrys-Kalemba , Helena Masiuk , Danuta Czernomysy-Furowicz , Paweł Nawrotek
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J. Microbiol. 2014;52(5):366-372. Published online April 11, 2014
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DOI: https://doi.org/10.1007/s12275-014-3436-2
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The purpose of this study was to analyze and compare genes encoding superantigens (SAgs) in Staphylococcus xylosus and Staphylococcus aureus isolates collected simultaneously from milk of the same cows with clinical mastitis. Genes encoding staphylococcal enterotoxins and enterotoxin-like proteins (sea-selu), toxic shock syndrome toxin 1 (tst-1) and exfolia-tive toxins (eta and etd) were investigated. It was found that among 30 isolates of S. xylosus, 16 (53.3%) harbored from 1 to 10 SAg genes. In total, in 16 SAg positive S. xylosus, 11 different enterotoxin genes were detected: sec, sed, seg, seh, sei, selm, seln, selo, selp, ser, selu and one etd gene encoding exfoliative toxin D. The most prevalent genes were ser, selu, and selo. Among all the positive isolates of S. xylosus, a total of 14 different SAg gene combinations were detected. One combination was repeated in 3 isolates, whereas the rest were detected only once. However, in the case of S. aureus all the 30 isolates harbored the same combination of SAg genes: seg, sei, selm, seln, selo and on the basis of PFGE analysis all belonged to the same clonal type. Also noteworthy was the observation that SAg genes detected in S. aureus have also been found in S. xylosus. The findings of this study further extend previous observations that SAg genes are present not only in S. aureus but also in coagulase-negative staphy-lococci, including S. xylosus. Therefore, taking into account that the SAg genes are encoded on mobile genetic elements it is possible that these genes can be transferred between different species of coexisting staphylococci.
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Reviews
- REVIEW] When a Virus is not a Parasite: The Beneficial Effects of Prophages
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Joseph Bondy-Denomy , Alan R. Davidson
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J. Microbiol. 2014;52(3):235-242. Published online March 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-4083-3
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Abstract
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Most organisms on the planet have viruses that infect them. Viral infection may lead to cell death, or to a symbiotic relationship where the genomes of both virus and host replicate together. In the symbiotic state, both virus and cell potentially experience increased fitness as a result of the other. The viruses that infect bacteria, called bacteriophages (or phages), well exemplify the symbiotic relationships that can develop between viruses and their host. In this review, we will discuss the many ways that prophages, which are phage genomes integrated into the genomes of their hosts, influence bacterial behavior and virulence.
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- MINIREVIEW] Toxin-producing Cyanobacteria in Freshwater: A Review of the Problems, Impact on Drinking Water Safety, and Efforts for Protecting Public Health
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Melissa Y. Cheung , Song Liang , Jiyoung Lee
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J. Microbiol. 2013;51(1):1-10. Published online March 2, 2013
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DOI: https://doi.org/10.1007/s12275-013-2549-3
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Abstract
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Cyanobacteria have adapted to survive in a variety of environments and have been found globally. Toxin-producing cyanobacterial harmful algal blooms (CHABs) have been increasing in frequency worldwide and pose a threat to drinking and recreational water. In this study, the prevalence, impact of CHABs and mitigation efforts were reviewed, focusing on the Lake Erie region and Ohio’s inland lakes that have been impacted heavily as an example so that the findings can be transferrable to other parts of the world that face the similar problems due to the CHABs in their freshwater environments. This paper provides a basic introduction to CHABs and their toxins as well as an overview of public health implications including exposure routes, health effects, and drinking water issues, algal bloom advisory practices in Ohio, toxin measurements results in Ohio public water supplies, and mitigation efforts.
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Environmental Health.2022;[Epub] CrossRef - Occurrence of cyanobacteria in water used for food production: A review
Mulalo Mutoti, Jabulani Gumbo, Afam Israel Obiefuna Jideani
Physics and Chemistry of the Earth, Parts A/B/C.2022; 125: 103101. CrossRef - Detection and Occurrence of Microcystins and Nodularins in Lake Manatee and Lake Washington-Two Floridian Drinking Water Systems
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Frontiers in Water.2022;[Epub] CrossRef - Evaluation of the Role of Probiotics As a New Strategy to Eliminate Microbial Toxins: a Review
Abdolamir Ghadaksaz, Somayeh Mousavi Nodoushan, Hamid Sedighian, Elham Behzadi, Abbas Ali Imani Fooladi
Probiotics and Antimicrobial Proteins.2022; 14(2): 224. CrossRef - Octocrylene: From Sunscreens to the Degradation Pathway during Chlorination Processes: Formation of Byproducts and Their Ecotoxicity Assessment
Antonio Medici, Lorenzo Saviano, Antonietta Siciliano, Giovanni Libralato, Marco Guida, Lucio Previtera, Giovanni Di Fabio, Armando Zarrelli
Molecules.2022; 27(16): 5286. CrossRef - Health impact assessment of air pollution in an area of the largest coal mine in Brazil
Laiz Coutelle Honscha, Julia Oliveira Penteado, Valério de Sá Gama, Alícia da Silva Bonifácio, Priscila Aikawa, Marina dos Santos, Paulo Roberto Martins Baisch, Ana Luíza Muccillo-Baisch, Flavio Manoel Rodrigues da Silva Júnior
Environmental Science and Pollution Research.2022; 29(10): 14176. CrossRef - Comparative genomic analysis of five freshwater cyanophages and reference-guided metagenomic data mining
Kang Du, Feng Yang, Jun-Tao Zhang, Rong-Cheng Yu, Ziqing Deng, Wei-Fang Li, Yuxing Chen, Qiong Li, Cong-Zhao Zhou
Microbiome.2022;[Epub] CrossRef - Prediction of Microcystis Occurrences and Analysis Using Machine Learning in High-Dimension, Low-Sample-Size and Imbalanced Water Quality Data
Masaya Mori, Roberto Gonzalez Flores, Yoshihiro Suzuki, Kei Nukazawa, Toru Hiraoka, Hirofumi Nonaka
Harmful Algae.2022; 117: 102273. CrossRef - Near‐term phytoplankton forecasts reveal the effects of model time step and forecast horizon on predictability
Whitney M. Woelmer, R. Quinn Thomas, Mary E. Lofton, Ryan P. McClure, Heather L. Wander, Cayelan C. Carey
Ecological Applications.2022;[Epub] CrossRef - Identifying the influence of zebra and quagga mussels on sedimentary phosphorus dynamics in western Lake Erie
Fasong Yuan, Robert A. Krebs, Amanda N. Wagner
Hydrobiologia.2021; 848(8): 1897. CrossRef - Effects of harmful algal blooms on regulated disinfection byproducts: Findings from five utility case studies
Katherine Foreman, Deborah Vacs Renwick, Morgan McCabe, Adam Cadwallader, Hannah Holsinger, Charlene Kormondy, Ryan Albert
AWWA Water Science.2021;[Epub] CrossRef - Rapid detection and quantification of microcystins in surface water by an impedimetric immunosensor
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Sensors and Actuators B: Chemical.2021; 348: 130687. CrossRef - Florida’s Harmful Algal Bloom (HAB) Problem: Escalating Risks to Human, Environmental and Economic Health With Climate Change
Cynthia Ann Heil, Amanda Lorraine Muni-Morgan
Frontiers in Ecology and Evolution.2021;[Epub] CrossRef - Killing effect of deinoxanthins on cyanobloom-forming Microcystis aeruginosa: Eco-friendly production and specific activity of deinoxanthins
Wonjae Kim, Minkyung Kim, Minyoung Hong, Woojun Park
Environmental Research.2021; 200: 111455. CrossRef - Design and Preliminary Testing of an In-Field Passive Treatment System for Removing Phosphorus from Surface Water
George Carleton, Jessica Glowczewski, Teresa Cutright
Applied Sciences.2021; 11(9): 3743. CrossRef - A machine learning approach for early warning of cyanobacterial bloom outbreaks in a freshwater reservoir
Yongeun Park, Han Kyu Lee, Jae-Ki Shin, Kangmin Chon, SungHwan Kim, Kyung Hwa Cho, Jin Hwi Kim, Sang-Soo Baek
Journal of Environmental Management.2021; 288: 112415. CrossRef - Harmful Cyanobacterial Blooms (HCBs): innovative green bioremediation process based on anti-cyanobacteria bioactive natural products
Soukaina El Amrani Zerrifi, Richard Mugani, El Mahdi Redouane, Fatima El Khalloufi, Alexandre Campos, Vitor Vasconcelos, Brahim Oudra
Archives of Microbiology.2021; 203(1): 31. CrossRef - Uncovering Research Trends of Phycobiliproteins Using Bibliometric Approach
Hui Teng Tan, Fatimah Md. Yusoff, Yam Sim Khaw, Siti Aqlima Ahmad, Noor Azmi Shaharuddin
Plants.2021; 10(11): 2358. CrossRef - Controlling Eutrophication via Surface Aerators in Irregular-Shaped Urban Ponds
Aimin Hao, Sohei Kobayashi, Dong Xia, Qi Mi, Ning Yan, Mengyao Su, Aishou Lin, Min Zhao, Yasushi Iseri
Water.2021; 13(23): 3360. CrossRef - Acute health effects associated with satellite-determined cyanobacterial blooms in a drinking water source in Massachusetts
Jianyong Wu, Elizabeth D. Hilborn, Blake A. Schaeffer, Erin Urquhart, Megan M. Coffer, Cynthia J. Lin, Andrey I. Egorov
Environmental Health.2021;[Epub] CrossRef - Acclimation of an algal consortium to sequester nutrients from anaerobic digestate
Qichen Wang, Jessa Cherones, Brendan Higgins
Bioresource Technology.2021; 342: 125921. CrossRef - Complete sequence and structure of the genome of the harmful algal bloom-forming cyanobacterium Planktothrix agardhii NIES-204T and detailed analysis of secondary metabolite gene clusters
Yohei Shimura, Takatomo Fujisawa, Yuu Hirose, Naomi Misawa, Yu Kanesaki, Yasukazu Nakamura, Masanobu Kawachi
Harmful Algae.2021; 101: 101942. CrossRef - Inhibition of endoplasmic reticulum stress-related autophagy attenuates MCLR-induced apoptosis in zebrafish testis and mouse TM4 cells
Zaiwei Wu, Fang Wang, Liwen Hu, Jianrong Zhang, Daojun Chen, Sujuan Zhao
Ecotoxicology and Environmental Safety.2021; 221: 112438. CrossRef - Expanding ecological assessment by integrating microorganisms into routine freshwater biomonitoring
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Water Research.2021; 191: 116767. CrossRef - Recent trends in the detection of freshwater cyanotoxins with a critical note on their occurrence in Asia
Prabir Kumar Kulabhusan, Katrina Campbell
Trends in Environmental Analytical Chemistry.2021; 32: e00150. CrossRef - Acute cyanotoxin poisoning reveals a marginal effect on mouse gut microbiome composition but indicates metabolic shifts related to liver and gut inflammation
Molly C. Mills, Morgan V. Evans, Seungjun Lee, Thomas Knobloch, Christopher Weghorst, Jiyoung Lee
Ecotoxicology and Environmental Safety.2021; 215: 112126. CrossRef - Quantifying Scales of Spatial Variability of Cyanobacteria in a Large, Eutrophic Lake Using Multiplatform Remote Sensing Tools
Samantha L. Sharp, Alexander L. Forrest, Keith Bouma-Gregson, Yufang Jin, Alicia Cortés, S. Geoffrey Schladow
Frontiers in Environmental Science.2021;[Epub] CrossRef - Climate Change and Emerging Food Safety Issues: A Review
Ramona A. Duchenne-Moutien, Hudaa Neetoo
Journal of Food Protection.2021; 84(11): 1884. CrossRef - Assessing Impacts of Changes in External Nutrient Loadings on a Temperate Chinese Drinking Water Reservoir
Qingchuan Chou, Anders Nielsen, Tobias K. Andersen, Fenjuan Hu, Weiyu Chen, Xiaolin Zhang, Te Cao, Leyi Ni, Erik Jeppesen, Dennis Trolle
Frontiers in Environmental Science.2021;[Epub] CrossRef - Diatomite-enhanced coagulation for algal removal in polluted raw water: performance optimization and pilot-scale study
Chao Jiang, Wei Ding, Weitang Zhu, Liang Zhu, Xiangyang Xu
Environmental Science and Pollution Research.2021; 28(36): 50204. CrossRef - The Application of Potassium Permanganate to Treat Cyanobacteria-Laden Water: a Review
Kayla Piezer, Lei Li, Youchul Jeon, Anusha Kadudula, Youngwoo Seo
Process Safety and Environmental Protection.2021; 148: 400. CrossRef - LC and NMR Studies for Identification and Characterization of Degradation Byproducts of Olmesartan Acid, Elucidation of Their Degradation Pathway and Ecotoxicity Assessment
Giovanni Luongo, Antonietta Siciliano, Giovanni Libralato, Sara Serafini, Lorenzo Saviano, Lucio Previtera, Giovanni Di Fabio, Armando Zarrelli
Molecules.2021; 26(6): 1769. CrossRef - Quantifying the Human Health Benefits of Using Satellite Information to Detect Cyanobacterial Harmful Algal Blooms and Manage Recreational Advisories in U.S. Lakes
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GeoHealth.2020;[Epub] CrossRef - Synthesis of new tetronamides displaying inhibitory activity against bloom‐forming cyanobacteria
Jaime AM Acosta, Milandip Karak, Luiz CA Barbosa, John Boukouvalas, Andrea Straforini, Giuseppe Forlani
Pest Management Science.2020; 76(2): 779. CrossRef - Analysis of Covalently Bound Microcystins in Sediments and Clam Tissue in the Sacramento–San Joaquin River Delta, California, USA
Melissa Bolotaolo, Tomofumi Kurobe, Birgit Puschner, Bruce G Hammock, Matt J. Hengel, Sarah Lesmeister, Swee J. Teh
Toxins.2020; 12(3): 178. CrossRef - Concise Synthesis of (+)‐[13C4]‐Anatoxin‐a by Dynamic Kinetic Resolution of a Cyclic Iminium Ion
Jacob J. Lacharity, Artur K. Mailyan, Karen Y. Chen, Armen Zakarian
Angewandte Chemie.2020; 132(28): 11460. CrossRef - Enhanced resistance of co-existing toxigenic and non-toxigenic Microcystis aeruginosa to pyrogallol compared with monostrains
Yunni Gao, Jing Lu, Philip T. Orr, Ann Chuang, Hannah M. Franklin, Michele A. Burford
Toxicon.2020; 176: 47. CrossRef - Drinking water treatment residuals from cyanobacteria bloom-affected areas: Investigation of potential impact on agricultural land application
Yuehan Ai, Seungjun Lee, Jiyoung Lee
Science of The Total Environment.2020; 706: 135756. CrossRef - Relationship between cyanobacterial bloom impacted drinking water sources and hepatocellular carcinoma incidence rates
Tyler Gorham, Elisabeth Dowling Root, Yuanyuan Jia, C.K. Shum, Jiyoung Lee
Harmful Algae.2020; 95: 101801. CrossRef - Microcystis Bloom in an Urban Lake after River Water Diversion—A Case Study
Xiaoyan Chen, Dong Bai, Chunlei Song, Yiyong Zhou, Xiuyun Cao
Water.2020; 12(6): 1811. CrossRef - Cyanobacterial blooms modify food web structure and interactions in western Lake Erie
Ruth D. Briland, Joshua P. Stone, Manjunath Manubolu, Jiyoung Lee, Stuart A. Ludsin
Harmful Algae.2020; 92: 101586. CrossRef - Evaluation of alum-based water treatment residuals used to adsorb reactive phosphorus
George Carleton, Teresa J. Cutright
Water Science and Engineering.2020; 13(3): 181. CrossRef - Exposure to microcystin among coastal residents during a cyanobacteria bloom in Florida
Adam M. Schaefer, Luke Yrastorza, Nicole Stockley, Kathi Harvey, Nancy Harris, Robert Grady, James Sullivan, Malcolm McFarland, John S. Reif
Harmful Algae.2020; 92: 101769. CrossRef - Suppression of water-bloom cyanobacterium Microcystis aeruginosa by algaecide hydrogen peroxide maximized through programmed cell death
Tingru Zhou, Huansheng Cao, Jie Zheng, Fei Teng, Xuejian Wang, Kai Lou, Xihui Zhang, Yi Tao
Journal of Hazardous Materials.2020; 393: 122394. CrossRef - Identifying the Influence of Land Cover and Human Population on Chlorophyll a Concentrations Using a Pseudo-Watershed Analytical Framework
Luke Moslenko, Kevin Blagrave, Alessandro Filazzola, Arnab Shuvo, Sapna Sharma
Water.2020; 12(11): 3215. CrossRef - Impact of Hydrogen Peroxide and Copper Sulfate on the Delayed Release of Microcystin
Arash Zamyadi, Katherine E. Greenstein, Caitlin M. Glover, Craig Adams, Erik Rosenfeldt, Eric C. Wert
Water.2020; 12(4): 1105. CrossRef - Concise Synthesis of (+)‐[13C4]‐Anatoxin‐a by Dynamic Kinetic Resolution of a Cyclic Iminium Ion
Jacob J. Lacharity, Artur K. Mailyan, Karen Y. Chen, Armen Zakarian
Angewandte Chemie International Edition.2020; 59(28): 11364. CrossRef - Harnessing the biocatalytic potential of iron- and α-ketoglutarate-dependent dioxygenases in natural product total synthesis
Christian R. Zwick, Hans Renata
Natural Product Reports.2020; 37(8): 1065. CrossRef - Environmental factors controlling phytoplankton dynamics in a large floodplain river with emphasis on cyanobacteria
Shawn M. Giblin, Gretchen A. Gerrish
River Research and Applications.2020; 36(7): 1137. CrossRef - Identification and enumeration of cyanobacteria species using a deep neural network
Sang-Soo Baek, JongCheol Pyo, Yakov Pachepsky, Yongeun Park, Mayzonee Ligaray, Chi-Yong Ahn, Young-Hyo Kim, Jong Ahn Chun, Kyung Hwa Cho
Ecological Indicators.2020; 115: 106395. CrossRef - Monitoring cyanobacteria occurrence in freshwater reservoirs using semi-analytical algorithms and orbital remote sensing
Henrique Dantas Borges, Rejane Ennes Cicerelli, Tati de Almeida, Henrique L. Roig, Diogo Olivetti
Marine and Freshwater Research.2020; 71(5): 569. CrossRef - Microcystis toxin-mediated tumor promotion and toxicity lead to shifts in mouse gut microbiome
Jiyoung Lee, Seungjun Lee, Alba Mayta, Igor Mrdjen, Christopher Weghorst, Thomas Knobloch
Ecotoxicology and Environmental Safety.2020; 206: 111204. CrossRef - Effect of Zinc on Microcystis aeruginosa UTEX LB 2385 and Its Toxin Production
Jose L. Perez, Tinchun Chu
Toxins.2020; 12(2): 92. CrossRef - Ecogenomics of the Marine Benthic Filamentous Cyanobacterium Adonisia
Juline M. Walter, Felipe H. Coutinho, Luciana Leomil, Paulo I. Hargreaves, Mariana E. Campeão, Verônica V. Vieira, Beatriz S. Silva, Giovana O. Fistarol, Paulo S. Salomon, Tomoo Sawabe, Sayaka Mino, Masashi Hosokawa, Hideaki Miyashita, Fumito Maruyama, Ma
Microbial Ecology.2020; 80(2): 249. CrossRef - Analysis of multiclass cyanotoxins (microcystins, anabaenopeptins, cylindrospermopsin and anatoxins) in lake waters using on-line SPE liquid chromatography high-resolution Orbitrap mass spectrometry
Audrey Roy-Lachapelle, Sung Vo Duy, Gabriel Munoz, Quoc Tuc Dinh, Emmanuelle Bahl, Dana F. Simon, Sébastien Sauvé
Analytical Methods.2019; 11(41): 5289. CrossRef - Design, synthesis and algicides activities of thiourea derivatives as the novel scaffold aldolase inhibitors
Shan Xiao, Lin Wei, Zongqin Hong, Li Rao, Yanliang Ren, Jian Wan, Lingling Feng
Bioorganic & Medicinal Chemistry.2019; 27(5): 805. CrossRef - Freshwater Supply to Metropolitan Shanghai: Issues of Quality from Source to Consumers
Maotian Li, Jing Chen, Brian Finlayson, Zhongyuan Chen, Michael Webber, Jon Barnett, Mark Wang
Water.2019; 11(10): 2176. CrossRef - Harmful algal blooms and liver diseases: focusing on the areas near the four major rivers in South Korea
Seungjun Lee, Jinnam Kim, Boseung Choi, Gijung Kim, Jiyoung Lee
Journal of Environmental Science and Health, Part C.2019; 37(4): 356. CrossRef - Characterization of Cyanophages in Lake Erie: Interaction Mechanisms and Structural Damage of Toxic Cyanobacteria
Xuewen Jiang, Chanhee Ha, Seungjun Lee, Jinha Kwon, Hanna Cho, Tyler Gorham, Jiyoung Lee
Toxins.2019; 11(8): 444. CrossRef - Nutrients, eutrophication and harmful algal blooms along the freshwater to marine continuum
Wayne A. Wurtsbaugh, Hans W. Paerl, Walter K. Dodds
WIREs Water.2019;[Epub] CrossRef - N-Halamine Derivatized Nanoparticles with Selective Cyanocidal Activity: Potential for Targeted Elimination of Harmful Cyanobacterial Blooms
Giji Sadhasivam, Chen Gelber, Varda Zakin, Shlomo Margel, Orr H. Shapiro
Environmental Science & Technology.2019; 53(15): 9160. CrossRef - Dynamics of cyanobacteria blooms are linked to the hydrology of shallow Florida lakes and provide insight into possible impacts of climate change
Karl E. Havens, Gaohua Ji, John R. Beaver, Rolland S. Fulton, Catherine E. Teacher
Hydrobiologia.2019; 829(1): 43. CrossRef - Microcystin-LR in peripheral circulation worsens the prognosis partly through oxidative stress in patients with hepatocellular carcinoma
Feifei Lei, Xu Lei, Rugui Li, Huabing Tan
Clinical and Experimental Medicine.2019; 19(2): 235. CrossRef - Economic impact of harmful algal blooms on human health: a systematic review
Christian R. C. Kouakou, Thomas G. Poder
Journal of Water and Health.2019; 17(4): 499. CrossRef - Influence of land use on trophic state indexes in northeast Brazilian river basins
Olandia Ferreira Lopes, Felizardo Adenilson Rocha, Lucas Farias de Sousa, Daniela Mariano Lopes da Silva, Andrique Figueiredo Amorim, Ronaldo Lima Gomes, André Luiz Sampaio da Silva Junior, Raildo Mota de Jesus
Environmental Monitoring and Assessment.2019;[Epub] CrossRef - Harmful algal bloom and associated health risks among users of Lake Victoria freshwater: Ukerewe Island, Tanzania
Geofrey J. Mchau, Edna Makule, Revocatus Machunda, Yun Yun Gong, Martin Kimanya
Journal of Water and Health.2019; 17(5): 826. CrossRef - Oriented Functionalization of Magnetic Beads with in Vivo Biotinylated Nanobodies for Rapid MALDI-TOF MS Ultrasensitive Quantitation of Microcystins in Biological Samples
Macarena Pírez-Schirmer, Beatriz M. Brena, Gualberto González-Sapienza
Analytical Chemistry.2019; 91(15): 9925. CrossRef - Alternate succession of aggregate-forming cyanobacterial genera correlated with their attached bacteria by co-pathways
Cong-Min Zhu, Jun-Yi Zhang, Rui Guan, Lauren Hale, Ning Chen, Ming Li, Zu-Hong Lu, Qin-Yu Ge, Yun-Feng Yang, Ji-Zhong Zhou, Ting Chen
Science of The Total Environment.2019; 688: 867. CrossRef - Identifying mechanisms of environmental decision-making: How ideology and geographic proximity influence public support for managing agricultural runoff to curb harmful algal blooms
Tian Guo, Erik C. Nisbet, Jay F. Martin
Journal of Environmental Management.2019; 241: 264. CrossRef - What determines the public’s support for water quality regulations to mitigate agricultural runoff?
Tian Guo, Devin Gill, Thomas H. Johengen, Bradley L. Cardinale
Environmental Science & Policy.2019; 101: 323. CrossRef - Relating Urban Biodiversity to Human Health With the ‘Holobiont’ Concept
Jacob G. Mills, Justin D. Brookes, Nicholas J. C. Gellie, Craig Liddicoat, Andrew J. Lowe, Harrison R. Sydnor, Torsten Thomas, Philip Weinstein, Laura S. Weyrich, Martin F. Breed
Frontiers in Microbiology.2019;[Epub] CrossRef - Biochemical and morphological biomarkers of the liver damage in the Neotropical fish, Piaractus mesopotamicus, injected with crude extract of cyanobacterium Radiocystis fernandoi
Driele Tavares, Marcelo Gustavo Paulino, Ana Paula Terezan, João Batista Fernandes, Alessandra Giani, Marisa Narciso Fernandes
Environmental Science and Pollution Research.2018; 25(16): 15349. CrossRef - Algal Blooms and Cyanotoxins in Jordan Lake, North Carolina
Daniel Wiltsie, Astrid Schnetzer, Jason Green, Mark Vander Borgh, Elizabeth Fensin
Toxins.2018; 10(2): 92. CrossRef - Heterologous expression and characterization of a novel serine protease from Daphnia magna: A possible role in susceptibility to toxic cyanobacteria
Jacqueline Lange, Fatih Demir, Pitter F. Huesgen, Ulrich Baumann, Eric von Elert, Christian Pichlo
Aquatic Toxicology.2018; 205: 140. CrossRef - High-Spatial Resolution Monitoring of Phycocyanin and Chlorophyll-a Using Airborne Hyperspectral Imagery
Jong Cheol Pyo, Mayzonee Ligaray, Yong Sung Kwon, Myoung-Hwan Ahn, Kyunghyun Kim, Hyuk Lee, Taegu Kang, Seong Been Cho, Yongeun Park, Kyung Hwa Cho
Remote Sensing.2018; 10(8): 1180. CrossRef - Seasonal Changes in Cyanobacterial Diversity of a Temperate Freshwater Paldang Reservoir (Korea) Explored by using Pyrosequencing
Thangavelu Boopathi, Hui Wang, Man-Duck Lee, Jang-Seu Ki
Environmental Biology Research.2018; 36(3): 424. CrossRef - Magnetic activated carbon@ iron oxide@manganese oxide composite as an adsorbent for preconcentration of microcystin –LR in surface water, tap water, water and wastewater
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Environmental Nanotechnology, Monitoring & Management.2018; 10: 199. CrossRef - Further Understanding of Degradation Pathways of Microcystin-LR by an Indigenous Sphingopyxis sp. in Environmentally Relevant Pollution Concentrations
Qin Ding, Kaiyan Liu, Kai Xu, Rongli Sun, Juan Zhang, Lihong Yin, Yuepu Pu
Toxins.2018; 10(12): 536. CrossRef - Solar Photocatalysis Using Fixed-Film TiO2 for Microcystins from Colonial Microcystis aeruginosa
Ciera M. Kinley, Maas Hendrikse, Alyssa J. Calomeni, Tyler D. Geer, John H. Rodgers
Water, Air, & Soil Pollution.2018;[Epub] CrossRef - Bloom-forming cyanobacteria and cyanotoxins in Argentina: A growing health and environmental concern
Anabella Aguilera, Signe Haakonsson, María Victoria Martin, Graciela L. Salerno, Ricardo O. Echenique
Limnologica.2018; 69: 103. CrossRef - Short Total Synthesis of [15N5]-Cylindrospermopsins from 15NH4Cl Enables Precise Quantification of Freshwater Cyanobacterial Contamination
Artur K. Mailyan, Joanna L. Chen, Weiwei Li, Arturo A. Keller, Shawn M. Sternisha, Brian G. Miller, Armen Zakarian
Journal of the American Chemical Society.2018; 140(18): 6027. CrossRef - Restoration of Nature or Special Interests? A Political Economy Analysis of the Four Major Rivers Restoration Project in South Korea
Hyojong Song, Michael J. Lynch
Critical Criminology.2018; 26(2): 251. CrossRef - The Effect of Organic Carbon Addition on the Community Structure and Kinetics of Microcystin-Degrading Bacterial Consortia
Derek Manheim, Yuen-Ming Cheung, Sunny Jiang
Water.2018; 10(11): 1523. CrossRef - Fresh produce and their soils accumulate cyanotoxins from irrigation water: Implications for public health and food security
Seungjun Lee, Xuewen Jiang, Manjunath Manubolu, Ken Riedl, Stuart A. Ludsin, Jay F. Martin, Jiyoung Lee
Food Research International.2017; 102: 234. CrossRef - Chlorophyll-A Prediction of Lakes with Different Water Quality Patterns in China Based on Hybrid Neural Networks
Xue Li, Jian Sha, Zhong-Liang Wang
Water.2017; 9(7): 524. CrossRef - Is the future of large shallow lakes blue-green? Comparing the response of a catchment-lake model chain to climate predictions
Fabien Cremona, Sirje Vilbaste, Raoul-Marie Couture, Peeter Nõges, Tiina Nõges
Climatic Change.2017; 141(2): 347. CrossRef - Disturbance‐mediated Cyanobacterial Harmful Algal Bloom (CHAB) formation in a small Ohio reservoir
Nathan Ruhl, Nicole Sullivan
Lakes & Reservoirs: Science, Policy and Management for Sustainable Use.2017; 22(2): 161. CrossRef - Natural Abenquines and Their Synthetic Analogues Exert Algicidal Activity against Bloom-Forming Cyanobacteria
Amalyn Nain-Perez, Luiz Cláudio Almeida Barbosa, Célia Regina Álvares Maltha, Giuseppe Forlani
Journal of Natural Products.2017; 80(4): 813. CrossRef - Sustainable Methods for Decontamination of Microcystin in Water Using Cold Plasma and UV with Reusable TiO2 Nanoparticle Coating
Xuewen Jiang, Seungjun Lee, Chulkyoon Mok, Jiyoung Lee
International Journal of Environmental Research and Public Health.2017; 14(5): 480. CrossRef - Cyanobacterial Toxins in Freshwater and Food: Important Sources of Exposure to Humans
Jiyoung Lee, Seungjun Lee, Xuewen Jiang
Annual Review of Food Science and Technology.2017; 8(1): 281. CrossRef - Spatiotemporal variability and environmental factors of harmful algal blooms (HABs) over western Lake Erie
Di Tian, Gengxin Xie, Jing Tian, Kuo-Hsin Tseng, C. K. Shum, Jiyoung Lee, Song Liang, Lucas J. Stal
PLOS ONE.2017; 12(6): e0179622. CrossRef - Host-genotype dependent gut microbiota drives zooplankton tolerance to toxic cyanobacteria
Emilie Macke, Martijn Callens, Luc De Meester, Ellen Decaestecker
Nature Communications.2017;[Epub] CrossRef - A perspective of chemical treatment for cyanobacteria control toward sustainable freshwater development
Jae-Hoon Huh, Ji-Whan Ahn
Environmental Engineering Research.2017; 22(1): 1. CrossRef - Alterations in transcription and protein expressions of HCC-related genes in HepG2 cells caused by microcystin-LR
Xiaoyu Li, Xiangyang Zhang, Wenjie Xie, Chune Zhou, Yao Li, Xiuhua Zhang
Toxicology in Vitro.2017; 40: 115. CrossRef - Ten-year survey of cyanobacterial blooms in Ohio’s waterbodies using satellite remote sensing
Tyler Gorham, Yuanyuan Jia, C.K. Shum, Jiyoung Lee
Harmful Algae.2017; 66: 13. CrossRef - Cyanobacterial Toxins of the Laurentian Great Lakes, Their Toxicological Effects, and Numerical Limits in Drinking Water
Todd Miller, Lucas Beversdorf, Chelsea Weirich, Sarah Bartlett
Marine Drugs.2017; 15(6): 160. CrossRef - Como as cidades podem favorecer ou dificultar a promoção da saúde de seus moradores?
Laís Fajersztajn, Mariana Veras, Paulo Hilário Nascimento Saldiva
Estudos Avançados.2016; 30(86): 7. CrossRef - A Rational Design, Synthesis, Biological Evaluation and Structure–Activity Relationship Study of Novel Inhibitors against Cyanobacterial Fructose-1,6-bisphosphate Aldolase
Xinya Han, Xiuyun Zhu, Shuaihua Zhu, Lin Wei, Zongqin Hong, Li Guo, Haifeng Chen, Bo Chi, Yan Liu, Lingling Feng, Yanliang Ren, Jian Wan
Journal of Chemical Information and Modeling.2016; 56(1): 73. CrossRef - Understanding the key ecological traits of cyanobacteria as a basis for their management and control in changing lakes
Evanthia Mantzouki, Petra M. Visser, Myriam Bormans, Bas W. Ibelings
Aquatic Ecology.2016; 50(3): 333. CrossRef - Health impacts from cyanobacteria harmful algae blooms: Implications for the North American Great Lakes
Wayne W. Carmichael, Gregory L. Boyer
Harmful Algae.2016; 54: 194. CrossRef - Hyperspectral remote sensing of cyanobacterial pigments as indicators of the iron nutritional status of cyanobacteria-dominant algal blooms in eutrophic lakes
Guangyu Chi, Jian Ma, Yi Shi, Xin Chen
Ecological Indicators.2016; 71: 609. CrossRef - Turbulence increases the risk of microcystin exposure in a eutrophic lake (Lake Taihu) during cyanobacterial bloom periods
Jian Zhou, Boqiang Qin, Xiaoxia Han, Lin Zhu
Harmful Algae.2016; 55: 213. CrossRef - Toxic cyanobacteria and drinking water: Impacts, detection, and treatment
Xuexiang He, Yen-Ling Liu, Amanda Conklin, Judy Westrick, Linda K. Weavers, Dionysios D. Dionysiou, John J. Lenhart, Paula J. Mouser, David Szlag, Harold W. Walker
Harmful Algae.2016; 54: 174. CrossRef - Anticancer compounds from cyanobacterium Lyngbya species: a review
Shasank S. Swain, Rabindra N. Padhy, Pawan K. Singh
Antonie van Leeuwenhoek.2015; 108(2): 223. CrossRef - Cyanobacteria blooms and non-alcoholic liver disease: evidence from a county level ecological study in the United States
Feng Zhang, Jiyoung Lee, Song Liang, CK Shum
Environmental Health.2015;[Epub] CrossRef - Effects of intermediate metabolite carboxylic acids of TCA cycle on Microcystis with overproduction of phycocyanin
Shijie Bai, Jingcheng Dai, Ming Xia, Jing Ruan, Hehong Wei, Dianzhen Yu, Ronghui Li, Hongmei Jing, Chunyuan Tian, Lirong Song, Dongru Qiu
Environmental Science and Pollution Research.2015; 22(7): 5531. CrossRef - Detection of the cyanobacterial toxin, microcystin-LR, using a novel recombinant antibody-based optical-planar waveguide platform
Caroline Murphy, Edwina Stack, Svetlana Krivelo, Daniel A. McPartlin, Barry Byrne, Charles Greef, Michael J. Lochhead, Greg Husar, Shauna Devlin, Christopher T. Elliott, Richard J. O’Kennedy
Biosensors and Bioelectronics.2015; 67: 708. CrossRef - Human Health-Related Ecosystem Services of Avian-Dense Coastal Wetlands Adjacent to a Western Lake Erie Swimming Beach
Chris L. Rea, Michael S. Bisesi, William Mitsch, Rebecca Andridge, Jiyoung Lee
EcoHealth.2015; 12(1): 77. CrossRef - Impact of Environmental Factors on the Regulation of Cyanotoxin Production
Thangavelu Boopathi, Jang-Seu Ki
Toxins.2014; 6(7): 1951. CrossRef - Neurotoxic action of microcystin-LR is reflected in the transcriptional stress response of Caenorhabditis elegans
Nadine Saul, Shumon Chakrabarti, Stephen R. Stürzenbaum, Ralph Menzel, Christian E.W. Steinberg
Chemico-Biological Interactions.2014; 223: 51. CrossRef - Female zebrafish (Danio rerio) are more vulnerable than males to microcystin-LR exposure, without exhibiting estrogenic effects
Qin Qiao, Wanjing Liu, Kang Wu, Ting Song, Jun Hu, Xiao Huang, Jing Wen, Liang Chen, Xuezhen Zhang
Aquatic Toxicology.2013; 142-143: 272. CrossRef
Research Support, Non-U.S. Gov'ts
- Heat- and Cold-Shock Responses in Fusarium graminearum 3 Acetyl- and 15 Acetyl-Deoxynivalenol Chemotypes
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Vladimir Vujanovic , Yit Kheng Goh , Prasad Daida
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J. Microbiol. 2012;50(1):97-102. Published online February 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1381-5
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146
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23
Scopus
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Abstract
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Fusarium graminearum Schwabe is the primary cause of
Fusarium head blight (FHB) in North America. Chemically
distinct F. graminearum sub-populations can be identified
based on the type or composition of deoxynivalenol (DON)
mycotoxin derivatives, including 3-acetyl (3-ADON) and
15-acetyl (15-ADON). The evaluation of randomly selected
3-ADON and 15-ADON isolates, collected from spring
wheat throughout Canada, was performed using thin layer
chromatography (TLC), high-performance liquid chromatography
(HPLC), ice-nucleation activity (INA), and heat and
cold tolerance tests conducted within a temperature range
of -70°C to 65°C. The results indicated that the 3-ADON
sub-population, which is responsible for the highest disease
severity and has rapidly displaced the 15-ADON sub-population,
produces more DON and zearalenone (ZEA) than
the 15-ADON sub-population when exposed to heat and
cold. Following exposures (1 and 2 h) to extremely high or
low temperatures, 3-ADON isolates exhibited faster mycelial
growth than 15-ADON isolates. In addition, the warmest
temperature at which INA activity occurred was in 3-ADON
(-3.6°C) vs. 15-ADON (-5.1°C). Taken together, these features
suggest that the newly emerging 3-ADON sub-population
is more resilient than the resident 15-ADON sub-population.
Overall, the differences between the two sub-populations
could provide new insights into FHB epidemiology and if
validated under field conditions, may provide important
information for predicting future FHB epidemics.
- Predicting Genetic Traits and Epitope Analysis of apxIVA in Actinobacillus pleuropneumoniae
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Min-Kyoung Shin , Seung-Bin Cha , Won-Jung Lee , Han Sang Yoo
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J. Microbiol. 2011;49(3):462-468. Published online June 30, 2011
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DOI: https://doi.org/10.1007/s12275-011-0449-y
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166
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7
Crossref
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Abstract
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Actinobacillus pleuropneumoniae causes a severe hemorrhagic pneumonia in pigs. Fifteen serotypes of A. pleuropneumoniae express four different Apx toxins that belong to the pore-forming repeats-in-toxin (RTX) group of toxins. ApxIV, which is conserved and up-regulated in vivo, could be an excellent candidate for the development of a protective cross-serotype immunity vaccine, and could aid in the differential diagnosis of diseases caused by A. pleuropneumoniae. We identified and sequenced apxIVA from A. pleuropneumoniae serotype 2 isolated in Korea (Kor-ApxIVA). The Kor-ApxIVA was closely related to Switzerland (AF021919), China (CP000687), and China (GQ332268), showing 98.6%, 98.4%, and 97.2% amino acid homology, respectively. The level of amino acid homology, however, was higher than the nucleotide homology. The structural characteristics of ApxIVA showed RTX proteins, including N-terminal hydrophobic domains, signature sequences for potential acylation sites, and repeated glycine-rich nonapeptides in the C-terminal region of the protein. Thirty glycine-rich nonapeptides with the consensus sequence, L/V-X-G-G-X-G-N/D-D-X, were found in the C-terminus of the Kor-ApxIVA. In addition, the Kor-ApxIVA was predicted for the linear B-cell epitopes and conserved domains with determined peptide sequences. This genetic analysis of the Kor-ApxIVA might be an important foundation for future biological and functional research on ApxIVA.
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Citations
Citations to this article as recorded by

- Review of advanced research on swine Actinobacillus pleuropneumoniae vaccine development strategy
Adehanom Baraki Tesfaye, Rui Han, Zhengyu Tao, Liuchao You, Jiayao Zhu, Pengcheng Gao, Lei Fu, Yuefeng Chu
Frontiers in Immunology.2025;[Epub] CrossRef - Rapid detection of Actinobacillus pleuropneumoniae targeting the apxIVA gene for diagnosis of contagious porcine pleuropneumonia in pigs by polymerase spiral reaction
R. Sarkar, P. Roychoudhury, S. Kumar, S. Dutta, N. Konwar, P. K. Subudhi, T. K. Dutta
Letters in Applied Microbiology.2022; 75(2): 442. CrossRef - Development ofActinobacillus pleuropneumoniaeApxI, ApxII, and ApxIII-specific ELISA methods for evaluation of vaccine efficiency
Myunghwan Jung, Hokeun Won, Min-Kyoung Shin, Myung Whan Oh, Soojin Shim, Injoong Yoon, Han Sang Yoo
Journal of Veterinary Science.2019;[Epub] CrossRef - Carbon source utilisation and evaluation of the Biolog system in the identification of Actinobacillus pleuropneumoniae
László Makrai, Rita Sárközi, László Fodor
Acta Veterinaria Hungarica.2019; 67(3): 327. CrossRef - So sánh khả năng dự đoán chất lượng nước sông Đồng Nai bằng mạng nơ ron nhân tạo và lý thuyết xám
Nguyễn Hiền Thân, Chế Đình Lý, Phạm Văn Tất
Can Tho University, Journal of Science.2017; Môi trường: 120. CrossRef - Prevalence and Characterization ofActinobacillus pleuropneumoniaeIsolated from Korean Pigs
Ki-Eun Lee, Hwan-Won Choi, Ha-Hyun Kim, Jae-Young Song, Dong-Kun Yang
Journal of Bacteriology and Virology.2015; 45(1): 19. CrossRef - Identification and Characterization of the Insecticidal Toxin “Makes Caterpillars Floppy” in Photorhabdus temperata M1021 Using a Cosmid Library
Ihsan Ullah, Eun-Kyung Jang, Min-Sung Kim, Jin-Ho Shin, Gun-Seok Park, Abdur Khan, Sung-Jun Hong, Byung-Kwon Jung, JungBae Choi, YeongJun Park, Yunyoung Kwak, Jae-Ho Shin
Toxins.2014; 6(7): 2024. CrossRef
- Use of Clostridium septicum Alpha Toxins for Isolation of Various Glycosylphosphatidylinositol-Deficient Cells
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Dong-Jun Shin , Hyon E. Choy , Yeongjin Hong
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J. Microbiol. 2005;43(3):266-271.
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DOI: https://doi.org/2214 [pii]
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Abstract
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In eukaryotic cells, various proteins are anchored to the plasma membrane through glycosylphosphatidylinositol (GPI). To study the biosynthetic pathways and modifications of GPI, various mutant cells have been isolated from the cells of Chinese hamster ovaries (CHO) supplemented with several exogenous genes involved in GPI biosynthesis using aerolysin, a toxin secreted from gram-negative bacterium Aeromonas hydrophila. Alpha toxin from Gram-positive bacterium Clostridium septicum is homologous to large lobes (LL) of aerolysin, binds GPI-anchored proteins and possesses a cell-destroying mechanism similar to aerolysin. Here, to determine whether alpha toxins can be used as an isolation tool of GPI-mutants, like aerolysin, CHO cells stably transfected with several exogenous genes involved in GPI biosynthesis were chemically mutagenized and cultured in a medium containing alpha toxins. We isolated six mutants highly resistant to alpha toxins and deficient in GPI biosynthesis. By genetic complementation, we determined that one mutant cell was defective of the second subunit of dolichol phosphate mannose synthase (DPM2) and other five cells were of a putative catalytic subunit of inositol acyltransferase (PIG-W). Therefore, C. septicum alpha toxins are a useful screening probe for the isolation of various GPI-mutant cells.