Journal of Microbiology

Restriction and Transcription Maps of Mitochondrial DNA of Trimorphomyces papilionaceus: Jeoung, Won Jin , Hong, Soon Gyu , Kang, Young Won , Jung, Hack Sung; J. Microbiol. 1995;33(2):149-153.

Abstract: Mitochondrial DNA has been isolated from Trimorphomyces papilionaceus. By analyzing DNA fragments digested by restriction enzymes, a restriction site map has been constructured. The mtDNA of T. papilionaceus amounts to 48.5 kb in size and is circular in structure. Entire mitochondrial DNA was cloned in E coli plasmids and Northern blot hybridization was done using cloned and subcloned DNAs as probes. Based on hybridization results of mitochondrial RNA transcripts, a transcription map was prepared.

Sequence Analysis of the Cytochrome b Gene of Trimorphomyces papilionaceus Mitochondria: Kim, Young Hyun , Kang, Young Won , Jung, Hack Sung; J. Microbiol. 1995;33(1):5-9.

Abstract: The DNA sequence of the cytochrome b (cob) gene region of Trimorphomyces papilionaceus mitochondrial DNA has been determined. The cob gene is interrupted by an intron of 1267 bp, which has an open reading frame of 897 bp contiguous to to the upstream exon. The intron belongs to group IB and contains a reading frame with a GIY-10-YVG motif. The deduced amino acid sequence shows 52~60% homology with those of other reported fungi. The phylogenetic relationship of T. papilionaceus with Neurospora crassa, Podospora adserina, Aspergillus nidulans, Saccharomyces cerevisiae, Schizosaccharomyces pombe, and Homo sapiens were inferred through sequence alignment and homology comparison of amino acids deduced from cob genes. When Homo sapiens was set as an outgroup, two ascomycetous yeasts S. cerevisiae and S. pombe made one group and three ascomycetous fungi N. crassa, P. anserine, and a basidiomycetous fungus T. papilionaceus the other group, suggesting that the former group evolved first and then the latter group separated into ascomycetous and basidiomycetous fungi right after that.

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