Journal of Microbiology

Letter

Proposal of Flavihumibacter fluvii sp. nov. as a replacement name for the effectively published but invalidated epithet Flavihumibacter fluminis Park et al. 2022: Miri S. Park , Hyeonuk Sa , Ilnam Kang , Jang-Cheon Cho; J. Microbiol. 2023;61(6):649-651. Published online June 12, 2023; DOI: https://doi.org/10.1007/s12275-023-00057-1

56 View
0 Download
1 Web of Science
1 Crossref

The name Flavihumibacter fluminis Park et al. 2022, which was effectively published but invalidated, is an illegitimate homonymic epithet of Flavihumibacter fluminis Guo et al. 2023. The low 16S rRNA gene sequence similarity and genomic relatedness between the type strains IMCC34837T and RY-1T of the two homonymic species indicated that they are different species. To avoid further confusion, we propose a new name Flavihumibacter fluvii sp. nov. to replace the effectively published but invalidated homonymic epithet Flavihumibacter fluminis Park et al. 2022.

Citations

Citations to this article as recorded by

Validation List no. 214. Valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, Markus Göker
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef

Journal Articles

Identification and Characterization of HEPN‑MNT Type II TA System from Methanothermobacter thermautotrophicus ΔH: Wonho Choi , Anoth Maharjan , Hae Gang Im , Ji-Young Park , Jong-Tae Park , Jung-Ho Park; J. Microbiol. 2023;61(4):411-421. Published online April 18, 2023; DOI: https://doi.org/10.1007/s12275-023-00041-9

55 View
0 Download
1 Web of Science
1 Crossref

Abstract

Toxin-antitoxin (TA) systems are widespread in bacteria and archaea plasmids and genomes to regulate DNA replication, gene transcr!ption, or protein translation. Higher eukaryotic and prokaryotic nucleotide-binding (HEPN) and minimal nucleotidyltransferase (MNT) domains are prevalent in prokaryotic genomes and constitute TA pairs. However, three gene pairs (MTH304/305, 408/409, and 463/464) of Methanothermobacter thermautotropicus ΔH HEPN-MNT family have not been studied as TA systems. Among these candidates, our study characterizes the MTH463/MTH464 TA system. MTH463 expression inhibited Escherichia coli growth, whereas MTH464 did not and blocked MTH463 instead. Using site-directed MTH463 mutagenesis, we determined that amino acids R99G, H104A, and Y106A from the R[ɸX]4-6H motif are involved with MTH463 cell toxicity. Furthermore, we established that purified MTH463 could degrade MS2 phage RNA, whereas purified MTH464 neutralized MTH463 activity in vitro. Our results indicate that the endonuclease toxin MTH463 (encoding a HEPN domain) and its cognate antitoxin MTH464 (encoding the MNT domain) may act as a type II TA system in M. thermautotropicus ΔH. This study provides initial and essential information studying TA system functions, primarily archaea HEPN-MNT family.

Citations

Citations to this article as recorded by

Extensive Genomic Rearrangement of Catalase-Less Cyanobloom-Forming Microcystis aeruginosa in Freshwater Ecosystems
Minkyung Kim, Jaejoon Jung, Wonjae Kim, Yerim Park, Che Ok Jeon, Woojun Park
Journal of Microbiology.2024; 62(11): 933. CrossRef

Extracellular vesicles derived from Lactobacillus plantarum restore chemosensitivity through the PDK2-mediated glucose metabolic pathway in 5-FU-resistant colorectal cancer cells: JaeJin An , Eun-Mi Ha; J. Microbiol. 2022;60(7):735-745. Published online July 4, 2022; DOI: https://doi.org/10.1007/s12275-022-2201-1

62 View
0 Download
18 Web of Science
16 Crossref

Abstract

Metabolic abnormalities are one of the main hallmarks of cancer and are associated with chemoresistance. Therefore, targeting the metabolic reprogramming of cancer cells has the potential to overcome chemoresistance. Probiotic-derived extracellular vesicles (EVs) play important roles in biological function and intracellular communication. However, the inhibitory effect of Lactobacillus plantarum-derived EVs (LpEVs) on colorectal cancer (CRC) cells has not yet been elucidated. This study clearly revealed that increased glycolysis in 5-fluorouracil (5-FU)-resistant CRC cells (CRC/5FUR) is directly related to chemoresistance and that the metabolic shift reversed by LpEVs inhibits cancer cell proliferation and eventually leads to apoptosis. Pyruvate dehydrogenase kinase 2 (PDK2), one of the crucial enzymes for enhancing glycolysis, was upregulated in CRC/5FUR cells. In our study, LpEVs sensitized CRC/5FUR cells to 5-FU by attenuating PDK2 expression in p53-p21-dependent metabolic signaling, thereby circumventing 5-FU resistance. We demonstrated the effect of cellular responses to 5-FU by modifying the PDK2 expression level in both 5-FU-sensitive parental CRC and 5- FU resistant CRC cell lines. Finally, we revealed that the PDK2 signaling pathway can potentially be targeted using LpEVs treatment to overcome chemoresistant CRC, thereby providing a potential strategy for CRC treatment by intervening in tumor metabolism.

Citations

Citations to this article as recorded by

Effect of probiotic extracellular vesicles and their applications on health and disease
Guangzhao Wang, Yang Wang, Kangliang Sheng, Yongzhong Wang
Journal of the Science of Food and Agriculture.2025;[Epub] CrossRef
Incorporation of recombinant proteins into extracellular vesicles by Lactococcus cremoris
Tina Vida Plavec, Kristina Žagar Soderžnik, Giulia Della Pelle, Špela Zupančič, Robert Vidmar, Aleš Berlec
Scientific Reports.2025;[Epub] CrossRef
The benefits of Lactiplantibacillus plantarum: From immunomodulator to vaccine vector
Joshua Tobias, Stefan Heinl, Kristina Dendinovic, Ajša Ramić, Anna Schmid, Catherine Daniel, Ursula Wiedermann
Immunology Letters.2025; 272: 106971. CrossRef
Interconnections within the tumor microenvironment: extracellular vesicles as critical players of metabolic reprogramming in tumor cells
Carol Costa Encarnação, Giselle Marianne Faria, Victor Aguiar Franco, Luiz Gabriel Xavier Botelho, João Alfredo Moraes, Mariana Renovato-Martins
Journal of Cancer Metastasis and Treatment.2024;[Epub] CrossRef
Review of METTL3 in colorectal cancer: From mechanisms to the therapeutic potential
Lexuan Zhang, Zhenwei Mao, Kai Yin, Shengjun Wang
International Journal of Biological Macromolecules.2024; 277: 134212. CrossRef
Extracellular Vesicles from Lacticaseibacillus Paracasei PC-H1 Inhibit HIF-1α-Mediated Glycolysis of Colon Cancer
Yangqian Shi, Chunliang Zhang, Wanyu Cao, Luyi Li, Kaili Liu, Hanyue Zhu, Fikadu Balcha, Yong Fang
Future Microbiology.2024; 19(3): 227. CrossRef
Role of probiotic extracellular vesicles in inter-kingdom communication and current technical limitations in advancing their therapeutic utility
Rahul Sanwlani, Kyle Bramich, Suresh Mathivanan
Extracellular Vesicles and Circulating Nucleic Acids.2024; : 609. CrossRef
Beneficial microbiome and diet interplay in early-onset colorectal cancer
Zhengyuan Zhou, Linda Kleis, Ana Depetris-Chauvin, Stefanie Jaskulski, Victoria Damerell, Karin B Michels, Biljana Gigic, Ute Nöthlings, Gianni Panagiotou
EMBO Molecular Medicine.2024; 17(1): 9. CrossRef
Deciphering the role of host-gut microbiota crosstalk via diverse sources of extracellular vesicles in colorectal cancer
Yun Song, Min Shi, Yugang Wang
Molecular Medicine.2024;[Epub] CrossRef
Targeting the gut and tumor microbiome in cancer treatment resistance
Sona Ciernikova, Aneta Sevcikova, Michal Mego
American Journal of Physiology-Cell Physiology.2024; 327(6): C1433. CrossRef
Lactobacillus plantarum Metabolites Elicit Anticancer Effects by Inhibiting Autophagy-Related Responses
Sihyun Jeong, Yuju Kim, Soyeong Park, Doyeon Lee, Juho Lee, Shwe Phyu Hlaing, Jin-Wook Yoo, Sang Hoon Rhee, Eunok Im
Molecules.2023; 28(4): 1890. CrossRef
Extracellular Vesicles of Probiotics: Shedding Light on the Biological Activity and Future Applications
Paweł Krzyżek, Beatrice Marinacci, Irene Vitale, Rossella Grande
Pharmaceutics.2023; 15(2): 522. CrossRef
Isolation and Characterization of Cow-, Buffalo-, Sheep- and Goat-Milk-Derived Extracellular Vesicles
Monisha Samuel, Rahul Sanwlani, Mohashin Pathan, Sushma Anand, Ella L. Johnston, Ching-Seng Ang, Maria Kaparakis-Liaskos, Suresh Mathivanan
Cells.2023; 12(20): 2491. CrossRef
Gut microbiota in colorectal cancer development and therapy
Chi Chun Wong, Jun Yu
Nature Reviews Clinical Oncology.2023; 20(7): 429. CrossRef
Phytochemicals targeting glycolysis in colorectal cancer therapy: effects and mechanisms of action
Lu Zhan, Fangting Su, Qiang Li, Yueqiang Wen, Feng Wei, Zhelin He, Xiaoyan Chen, Xiang Yin, Jian Wang, Yilin Cai, Yuxia Gong, Yu Chen, Xiao Ma, Jinhao Zeng
Frontiers in Pharmacology.2023;[Epub] CrossRef
Crosstalk between gut microbiota and RNA N6-methyladenosine modification in cancer
Hao Su, Henley Cheung, Harry Cheuk-Hay Lau, Hongyan Chen, Xiaoting Zhang, Na Qin, Yifei Wang, Matthew Tak Vai Chan, William Ka Kei Wu, Huarong Chen
FEMS Microbiology Reviews.2023;[Epub] CrossRef

Characterization of a cold-adapted debranching enzyme and its role in glycogen metabolism and virulence of Vibrio vulnificus MO6-24/O: Ah-Reum Han , Haeyoung Kim , Jong-Tae Park , Jung-Wan Kim; J. Microbiol. 2022;60(4):375-386. Published online February 14, 2022; DOI: https://doi.org/10.1007/s12275-022-1507-3

59 View
0 Download
4 Web of Science
5 Crossref

Abstract

Vibrio vulnificus MO6-24/O has three genes annotated as debranching enzymes or pullulanase genes. Among them, the gene encoded by VVMO6_03032 (vvde1) shares a higher similarity at the amino acid sequence level to the glycogen debranching enzymes, AmyX of Bacillus subtilis (40.5%) and GlgX of Escherichia coli (55.5%), than those encoded by the other two genes. The vvde1 gene encoded a protein with a molecular mass of 75.56 kDa and purified Vvde1 efficiently hydrolyzed glycogen and pullulan to shorter chains of maltodextrin and maltotriose (G3), respectively. However, it hydrolyzed amylopectin and soluble starch far less efficiently, and β-cyclodextrin (β-CD) only rarely. The optimal pH and temperature of Vvde1 was 6.5 and 25°C, respectively. Vvde1 was a cold-adapted debranching enzyme with more than 60% residual activity at 5°C. It could maintain stability for 2 days at 25°C and 1 day at 35°C, but it destabilized drastically at 40°C. The Vvde1 activity was inhibited considerably by Cu2+, Hg2+, and Zn2+, while it was slightly enhanced by Co2+, Ca2+, Ni2+, and Fe2+. The vvde1 knock-out mutant accumulated more glycogen than the wild-type in media supplemented with 1.0% maltodextrin; however, the side chain length distribution of glycogen was similar to that of the wild-type except G3, which was much more abundant in the mutant. Therefore, Vvde1 seemed to debranch glycogen with the degree of polymerization 3 (DP3) as the specific target branch length. Virulence of the pathogen against Caenorhabditis elegans was attenuated significantly by the vvde1 mutation. These results suggest that Vvde1 might be a unique glycogen debranching enzyme that is involved in both glycogen utilization and shaping of glycogen molecules, and contributes toward virulence of the pathogen.

Citations

Citations to this article as recorded by

Characterization of glycogen-related glycoside hydrolase glgX and glgB from Klebsiella pneumoniae and their roles in biofilm formation and virulence
Xinyue Liu, Jialin Li, Ruibing Wu, Liping Bai
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Function of the mdxR gene encoding a novel regulator for carbohydrate metabolism and sporulation in Bacillus subtilis 168
Tianshi Wang, Jung-Wan Kim
Archives of Microbiology.2023;[Epub] CrossRef
Identification of a novel cyclomaltodextrinase annotated as a neopullulanase in the genome of Bacillus cereus
Bo-Ram Park, Davoodbasha MubarakAli, Jung-Wan Kim
Archives of Microbiology.2023;[Epub] CrossRef
Functional conservation of specialized ribosomes bearing genome-encoded variant rRNAs in Vibrio species
Younkyung Choi, Eunkyoung Shin, Minho Lee, Ji-Hyun Yeom, Kangseok Lee, Bashir Sajo Mienda
PLOS ONE.2023; 18(12): e0289072. CrossRef
Functional characterization of maltodextrin glucosidase for maltodextrin and glycogen metabolism in Vibrio vulnificus MO6-24/O
Hye-Young Kim, MubarakAli Davoodbasha, Jung-Wan Kim
Archives of Microbiology.2022;[Epub] CrossRef

Vibrio vulnificus PlpA facilitates necrotic host cell death induced by the pore forming MARTX toxin: Changyi Cho , Sanghyeon Choi , Myung Hee Kim , Byoung Sik Kim; J. Microbiol. 2022;60(2):224-233. Published online February 1, 2022; DOI: https://doi.org/10.1007/s12275-022-1448-x

56 View
0 Download
7 Web of Science
6 Crossref

Abstract

Opportunistic pathogen Vibrio vulnificus causes severe systemic infection in humans with high mortality. Although multiple exotoxins have been characterized in V. vulnificus, their interactions and potential synergistic roles in pathogen-induced host cell death have not been investigated previously. By employing a series of multiple exotoxin deletion mutants, we investigated whether specific exotoxins of the pathogen functioned together to achieve severe and rapid necrotic cell death. Human epithelial cells treated with V. vulnificus with a plpA deletion background exhibited an unusually prolonged cell blebbing, suggesting the importance of PlpA, a phospholipase A2, in rapid necrotic cell death by this pathogen. Additional deletion of the rtxA gene encoding the multifunctional autoprocessing repeats-in-toxin (MARTX) toxin did not result in necrotic cell blebs. However, if the rtxA gene was engineered to produce an effector-free MARTX toxin, the cell blebbing was observed, indicating that the pore forming activity of the MARTX toxin is sufficient, but the MARTX toxin effector domains are not necessary, for the blebbing. When a recombinant PlpA was treated on the blebbed cells, the blebs were completely disrupted. Consistent with this, MARTX toxin-pendent rapid release of cytosolic lactate dehydrogenase was significantly delayed in the plpA deletion background. Mutations in other exotoxins such as elastase, cytolysin/hemolysin, and/or extracellular metalloprotease did not affect the bleb formation or disruption. Together, these findings indicate that the pore forming MARTX toxin and the phospholipase A2, PlpA, cooperate sequentially to achieve rapid necrotic cell death by inducing cell blebbing and disrupting the blebs, respectively.

Citations

Citations to this article as recorded by

Genome-wide phenotypic profiling of transcription factors and identification of novel targets to control the virulence of Vibrio vulnificus
Dayoung Sung, Garam Choi, Minji Ahn, Hokyung Byun, Tae Young Kim, Hojun Lee, Zee-Won Lee, Ji Yong Park, Young Hyun Jung, Ho Jae Han, Sang Ho Choi
Nucleic Acids Research.2024;[Epub] CrossRef
Vibrio-infecting bacteriophages and their potential to control biofilm
Ana Cevallos-Urena, Jeong Yeon Kim, Byoung Sik Kim
Food Science and Biotechnology.2023; 32(12): 1719. CrossRef
Pathogenic Mechanism of Vibrio Vulnificus Infection
Kun Lu, Yang Li, Rui Chen, Hua Yang, Yong Wang, Wei Xiong, Fang Xu, Qijun Yuan, Haihui Liang, Xian Xiao, Renqiang Huang, Zhipeng Chen, Chunou Tian, Songqing Wang
Future Microbiology.2023; 18(6): 373. CrossRef
Functional conservation of specialized ribosomes bearing genome-encoded variant rRNAs in Vibrio species
Younkyung Choi, Eunkyoung Shin, Minho Lee, Ji-Hyun Yeom, Kangseok Lee, Bashir Sajo Mienda
PLOS ONE.2023; 18(12): e0289072. CrossRef
Complex regulatory networks of virulence factors in Vibrio vulnificus
Garam Choi, Sang Ho Choi
Trends in Microbiology.2022; 30(12): 1205. CrossRef
MARTX toxin of Vibrio vulnificus induces RBC phosphatidylserine exposure that can contribute to thrombosis
Han Young Chung, Yiying Bian, Kyung-Min Lim, Byoung Sik Kim, Sang Ho Choi
Nature Communications.2022;[Epub] CrossRef

Alcohol dehydrogenase 1 and NAD(H)-linked methylglyoxal oxidoreductase reciprocally regulate glutathione-dependent enzyme activities in Candida albicans: Sa-Ouk Kang , Min-Kyu Kwak; J. Microbiol. 2021;59(1):76-91. Published online December 23, 2020; DOI: https://doi.org/10.1007/s12275-021-0552-7

54 View
0 Download
2 Web of Science
2 Crossref

Abstract

Glutathione reductase (Glr1) activity controls cellular glutathione and reactive oxygen species (ROS). We previously demonstrated two predominant methylglyoxal scavengers– NAD(H)-linked methylglyoxal oxidoreductase (Mgd1) and alcohol dehydrogenase 1 (Adh1)–in glutathione-depleted γ- glutamyl cysteinyl synthetase-disrupted Candida albicans. However, experimental evidence for Candida pathophysiology lacking the enzyme activities of Mgd1 and Adh1 on glutathione- dependent redox regulation remains unclear. Herein, we have aimed to demonstrate that glutathione-dependent enzyme activities coupled with cellular ROS changes is regulated by methylglyoxal accumulation in Δmgd1/Δadh1 double disruptants. Δmgd1/Δadh1 showed severe growth defects and G1-phase cell cycle arrest. The observed complementary and reciprocal methylglyoxal-oxidizing and methylglyoxalreducing activities between Δmgd1 and Δadh1 were not always exhibited in Δmgd1/Δadh1. Although intracellular accumulation of methylglyoxal and pyruvate was shown in all disruptants, to a greater or lesser degree, methylglyoxal was particularly accumulated in the Δmgd1/Δadh1 double disruptant. While cellular ROS significantly increased in Δmgd1 and Δadh1 as compared to the wild-type, Δmgd1/Δadh1 underwent a decrease in ROS in contrast to Δadh1. Despite the experimental findings underlining the importance of the undergoing unbalanced redox state of Δmgd1/Δadh1, glutathione- independent antioxidative enzyme activities did not change during proliferation and filamentation. Contrary to the significantly lowered glutathione content and Glr1 enzyme activity, the activity staining-based glutathione peroxidase activities concomitantly increased in this mutant. Additionally, the enhanced GLR1 transcript supported our results in Δmgd1/Δadh1, indicating that deficiencies of both Adh1 and Mgd1 activities stimulate specific glutathione-dependent enzyme activities. This suggests that glutathione-dependent redox regulation is evidently linked to C. albicans pathogenicity under the control of methylglyoxal-scavenging activities.

Citations

Citations to this article as recorded by

Role of methylglyoxal and redox homeostasis in microbe-mediated stress mitigation in plants
Sampurna Garai, Bidisha Bhowal, Mayank Gupta, Sudhir K Sopory, Sneh L. Singla-Pareek, Ashwani Pareek, Charanpreet Kaur
Plant Science.2024; 338: 111922. CrossRef
Roles of alcohol dehydrogenase 1 in the biological activities of Candida albicans
Ziqi Wang, Qi Zhang, Haoying Zhang, Yuanyuan Lu
Critical Reviews in Microbiology.2024; : 1. CrossRef

Analyses of DNA double-strand break repair pathways in tandem arrays of HXT genes of Saccharomyces cerevisiae: Ju-Hee Choi , Ye-Seul Lim , Min-Ku Kim , Sung-Ho Bae; J. Microbiol. 2020;58(11):957-966. Published online October 30, 2020; DOI: https://doi.org/10.1007/s12275-020-0461-1

52 View
0 Download
4 Web of Science
4 Crossref

Abstract

Eukaryotic genomes contain numerous homologous repeat sequences including redundant genes with divergent homology that can be potential recombination targets. Recombination between divergent sequences is rare but poses a substantial threat to genome stability. The hexose transporter (HXT) gene family shares high sequence similarities at both protein and DNA levels, and some members are placed close together in tandem arrays. In this study, we show that spontaneous interstitial deletions occur at significantly high rates in HXT gene clusters, resulting in chimeric HXT sequences that contain a single junction point. We also observed that DNA double-strand breaks created between HXT genes produce primarily interstitial deletions, whereas internal cleavage of the HXT gene resulted in gene conversions as well as deletion products. Interestingly, interstitial deletions were less constrained by sequence divergence than gene conversion. Moreover, recombination-defective mutations differentially affected the survival frequency. Mutations that impair single-strand annealing (SSA) pathway greatly reduced the survival frequency by 10–1,000-fold, whereas disruption of Rad51-dependent homologous recombination exhibited only modest reduction. Our results indicate that recombination in the tandemly repeated HXT genes occurs primarily via SSA pathway.

Citations

Citations to this article as recorded by

Deletion of IRC19 Causes Defects in DNA Double-Strand Break Repair Pathways in Saccharomyces cerevisiae
Ju-Hee Choi, Oyungoo Bayarmagnai, Sung-Ho Bae
Journal of Microbiology.2024; 62(9): 749. CrossRef
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris
Xiang Wang, Yi Li, Zhehao Jin, Xiangjian Liu, Xiang Gao, Shuyuan Guo, Tao Yu
Synthetic and Systems Biotechnology.2023; 8(3): 445. CrossRef
Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms
Jucan Gao, Cuifang Ye, Jintao Cheng, Lihong Jiang, Xinghao Yuan, Jiazhang Lian
ACS Synthetic Biology.2022; 11(2): 547. CrossRef
Effects of the loss of mismatch repair genes on single-strand annealing between divergent sequences in Saccharomyces cerevisiae
Ye-Seul Lim, Ju-Hee Choi, Kyu-Jin Ahn, Min-Ku Kim, Sung-Ho Bae
Journal of Microbiology.2021; 59(4): 401. CrossRef

IgG and IgM responses to human papillomavirus L1 virus-like particle as a function of dosing schedule and vaccine formulation: Min-Hye Park , Ji Won You , Hyoung Jin Kim , Hong-Jin Kim; J. Microbiol. 2019;57(9):821-827. Published online August 27, 2019; DOI: https://doi.org/10.1007/s12275-019-9308-z

52 View
0 Download
4 Web of Science
4 Crossref

Abstract

Most commercialized virus-like particle (VLP) vaccines use aluminum salt as adjuvant, even though VLPs provoke adequate antibody responses without adjuvant. We do not have detailed knowledge of how adjuvant affects the profile of anti- VLP antibodies. Meanwhile, there is evidence that differences between vaccination protocols influence the glycosylation of antibodies, which may alter their effector functions. In the present study a murine model was used to investigate the effects of dosing schedule and adjuvant on the antibody profiles and glycosylation levels of antigen-specific antibody responses to human papillomavirus type 16 L1 (HPV16 L1) VLPs. Mice received subcutaneously 2,000 ng of antigen divided into 4 or 7 doses. The HPV16 L1 VLPs elicited > 4 log10 anti-HPV16 L1 IgG titers without adjuvant, and aluminum hydroxide as adjuvant increased IgG titers 1.3- to 4-fold and reduced the anti-HPV16 L1 IgG2a / anti-HPV16 L1 IgG1 ratio value (use of aluminum hydroxide reduced the ratio of the IgG2a). Immunization with HPV16 L1 VLPs in combination with Freund’s adjuvant enhanced IgG titers 5- to 12- fold. Seven-dose immunization markedly increased anti- HPV16 L1 IgM titers compared to four-dose immunization, as well as increasing the proportion of glycosylated antibodies. Our results suggest that antibody glycosylation can be controlled immunologically, and IgG and IgM profiles and glycosylation profiles of the vaccine-induced antibodies can be used as indicators reflecting the vaccine characteristics. These
results
indicate that the HPV16 L1 VLP dosing schedule can affect the quality of antigen-specific antibody responses. We suggest that dosing schedules should be noted in vaccination protocols for VLP-based vaccines.

Citations

Citations to this article as recorded by

Human papillomavirus vaccines: organisation and experience of preclinical studies
A. S. Korovkin, T. N. Nikitina, T. Yu. Kozlova, D. V. Gorenkov, A. R. Volgin
Biological Products. Prevention, Diagnosis, Treatment.2024; 24(3): 243. CrossRef
Chimeric Hepatitis B core virus-like particles harboring SARS-CoV2 epitope elicit a humoral immune response in mice
Sima Sazegari, Malihe Akbarzadeh Niaki, Alireza Afsharifar, Ali Niazi, Abdollah Derakhshandeh, Maryam Moradi Vahdat, Farshad Hemmati, Mohammad Hadi Eskandari
Microbial Cell Factories.2023;[Epub] CrossRef
Anti-JMH alloantibody in inherited JMH-negative patients leads to immunogenic destruction of JMH-positive RBCs
Zhaohu Yuan, Yaming Wei, Xiaojie Chen, Shufei He, Kui Cai, Minglu Zhong, Huiying Huang, Xinxin Tong, Zhen Liu, Xuexin Yang
Clinical and Experimental Immunology.2021; 205(2): 182. CrossRef
Prevalence of antibodies against a cyclic peptide mimicking the FG loop of the human papillomavirus type 16 capsid among Tunisian women
Elham Hassen, Devendra Bansal, Randa Ghdira, Anouar Chaieb, Hedi Khairi, Abdelfattah Zakhama, Sami Remadi, Johan Hoebeke, Ali A. Sultan, Lotfi Chouchane
Journal of Translational Medicine.2020;[Epub] CrossRef

Analysis of IE62 mutations found in Varicella-Zoster virus vaccine strains for transactivation activity: Hyemin Ko , Gwang Myeong Lee , Ok Sarah Shin , Moon Jung Song , Chan Hee Lee , Young Eui Kim , Jin-Hyun Ahn; J. Microbiol. 2018;56(6):441-448. Published online June 1, 2018; DOI: https://doi.org/10.1007/s12275-018-8144-x

48 View
0 Download
2 Crossref

Abstract

Live attenuated vaccine strains have been developed for Varicella- Zoster virus (VZV). Compared to clinically isolated strains, the vaccine strains contain several non-synonymous mutations in open reading frames (ORFs) 0, 6, 31, 39, 55, 62, and 64. In particular, ORF62, encoding an immediate-early (IE) 62 protein that acts as a transactivator for viral gene expression, contains six non-synonymous mutations, but whether these mutations affect transactivation activity of IE62 is not understood. In this study, we investigated the role of non-synonymous vaccine-type mutations (M99T, S628G, R958G, V1197A, I1260V, and L1275S) of IE62 in Suduvax, a vaccine strain isolated in Korea, for transactivation activity. In reporter assays, Suduvax IE62 showed 2- to 4-fold lower transactivation activity toward ORF4, ORF28, ORF29, and ORF68 promoters than wild-type IE62. Introduction of individual M99T, S628G, R958G, or V1197A/ I1260V/L1275S mutations into wild-type IE62 did not affect transactivation activity. However, the combination of M99T within the N-terminal Sp transcription factor binding region and V1197A/I1260V/L1275S within the C-terminal serineenriched acidic domain (SEAD) significantly reduced the transactivation activity of IE62. The M99T/V1197A/I1260V/ L1275S mutant IE62 did not show considerable alterations in intracellular distribution and Sp3 binding compared to wild-type IE62, suggesting that other alteration(s) may be responsible for the reduced transactivation activity. Collectively, our results suggest that acquisition of mutations in both Met 99 and the SEAD of IE62 is responsible for the reduced transactivation activity found in IE62 of the VZV vaccine strains and contributes to attenuation of the virus.

Citations

Citations to this article as recorded by

Heightened incidence of adverse events associated with a live attenuated varicella vaccine strain that lacks critical genetic polymorphisms in open reading frame 62
Ye Ji Kim, Doyeop Oh, Jaehoon Kim, Jeongtae Son, Jae Yun Moon, Ye Kyung Kim, Bin Ahn, Kyu Ri Kang, Daechan Park, Hyun Mi Kang
Clinical Microbiology and Infection.2024; 30(11): 1466. CrossRef
Whole Transcriptome Analyses Reveal Differential mRNA and microRNA Expression Profiles in Primary Human Dermal Fibroblasts Infected with Clinical or Vaccine Strains of Varicella Zoster Virus
Soo-Jin Oh, Sooyeon Lim, Moon Jung Song, Jin Hyun Ahn, Chan Hee Lee, Ok Sarah Shin
Pathogens.2019; 8(4): 183. CrossRef

Review

[Minireview] Antibiotic resistance of pathogenic Acinetobacter species and emerging combination therapy: Bora Shin , Woojun Park; J. Microbiol. 2017;55(11):837-849. Published online October 27, 2017; DOI: https://doi.org/10.1007/s12275-017-7288-4

50 View
0 Download
38 Crossref

Abstract

The increasing antibiotic resistance of Acinetobacter species in both natural and hospital environments has become a serious problem worldwide in recent decades. Because of both intrinsic and acquired antimicrobial resistance (AMR) against last-resort antibiotics such as carbapenems, novel therapeutics are urgently required to treat Acinetobacter-associated infectious diseases. Among the many pathogenic Acinetobacter species, A. baumannii has been reported to be resistant to all classes of antibiotics and contains many AMR genes, such as blaADC (Acinetobacter-derived cephalosporinase). The AMR of pathogenic Acinetobacter species is the result of several different mechanisms, including active efflux pumps, mutations in antibiotic targets, antibiotic modification, and low antibiotic membrane permeability. To overcome the limitations of existing drugs, combination theraphy that can increase the activity of antibiotics should be considered in the treatment of Acinetobacter infections. Understanding the molecular mechanisms behind Acinetobacter AMR resistance will provide vital information for drug development and therapeutic strategies using combination treatment. Here, we summarize the classic mechanisms of Acinetobacter AMR, along with newly-discovered genetic AMR factors and currently available antimicrobial adjuvants that can enhance drug efficacy in the treatment of A. baumannii infections.

Citations

Citations to this article as recorded by

Disruption of bacterial interactions and community assembly in Babesia-infected Haemaphysalis longicornis following antibiotic treatment
Myriam Kratou, Apolline Maitre, Lianet Abuin-Denis, Elianne Piloto-Sardiñas, Ivan Corona-Guerrero, Ana Laura Cano-Argüelles, Alejandra Wu-Chuang, Timothy Bamgbose, Consuelo Almazan, Juan Mosqueda, Dasiel Obregón, Lourdes Mateos-Hernández, Mourad Ben Said,
BMC Microbiology.2024;[Epub] CrossRef
A 19-year longitudinal study to characterize carbapenem-nonsusceptible Acinetobacter isolated from patients with bloodstream infections and the contribution of conjugative plasmids to carbapenem resistance and virulence
Pek Kee Chen, Yi-Tzu Lee, Chia-Ying Liu, Tran Thi Dieu Thuy, Kieu Anh, Jiunn-Jong Wu, Chun-Hsing Liao, Yu-Tsung Huang, Yu-Chen Chen, Cheng-Yen Kao
Journal of Microbiology, Immunology and Infection.2024; 57(2): 288. CrossRef
MOLECULAR ANALYSIS OF THE MCR-1 GENE IN PSEUDOMONAS AERUGINOSA AND ACINETOBACTER BAUMANII STRAINS
Ömer Akgül
Ankara Universitesi Eczacilik Fakultesi Dergisi.2024; 48(3): 21. CrossRef
Effect of Phenylalanine–Arginine Beta-Naphthylamide on the Values of Minimum Inhibitory Concentration of Quinolones and Aminoglycosides in Clinical Isolates of Acinetobacter baumannii
Stefany Plasencia-Rebata, Saul Levy-Blitchtein, Juana del Valle-Mendoza, Wilmer Silva-Caso, Isaac Peña-Tuesta, William Vicente Taboada, Fernando Barreda Bolaños, Miguel Angel Aguilar-Luis
Antibiotics.2023; 12(6): 1071. CrossRef
A comprehensive genomic analysis provides insights on the high environmental adaptability of Acinetobacter strains
Yang Zhao, Hua-Mei Wei, Jia-Li Yuan, Lian Xu, Ji-Quan Sun
Frontiers in Microbiology.2023;[Epub] CrossRef
Acinetobacter baumannii in blood-borne and central nervous system infections in intensive care unit children: molecular and genetic characteristics and clinical significance
Zulfirya Z. Sadeeva, Irina E. Novikova, Natalia M. Alyabyeva, Anna V. Lazareva, Tatiana M. Komyagina, Olga V. Karaseva, Marina G. Vershinina, Andrey P. Fisenko
Russian Journal of Infection and Immunity.2023; 13(2): 289. CrossRef
Herbal Products and Their Active Constituents Used Alone and in Combination with Antibiotics against Multidrug-Resistant Bacteria
Anna Herman, Andrzej P. Herman
Planta Medica.2023; 89(02): 168. CrossRef
A Systematic Review of Culture-Based Methods for Monitoring Antibiotic-Resistant Acinetobacter, Aeromonas, and Pseudomonas as Environmentally Relevant Pathogens in Wastewater and Surface Water
Erin G. Milligan, Jeanette Calarco, Benjamin C. Davis, Ishi M. Keenum, Krista Liguori, Amy Pruden, Valerie J. Harwood
Current Environmental Health Reports.2023; 10(2): 154. CrossRef
Gold nanoparticle-DNA aptamer-assisted delivery of antimicrobial peptide effectively inhibits Acinetobacter baumannii infection in mice
Jaeyeong Park, Eunkyoung Shin, Ji-Hyun Yeom, Younkyung Choi, Minju Joo, Minho Lee, Je Hyeong Kim, Jeehyeon Bae, Kangseok Lee
Journal of Microbiology.2022; 60(1): 128. CrossRef
In vitro study to evaluate the antimicrobial activity of various multifunctional cosmetic ingredients and chlorphenesin on bacterial species at risk in the cosmetic industry
Benjamin Youenou, Amandine Chauviat, Chrisse Ngari, Valérie Poulet, Sylvie Nazaret
Journal of Applied Microbiology.2022; 132(2): 933. CrossRef
Profiles of Microbial Community and Antibiotic Resistome in Wild Tick Species
Nana Wei, Jinmiao Lu, Yi Dong, Shibo Li, Jack A. Gilbert
mSystems.2022;[Epub] CrossRef
Conventional and Real-Time PCR Targeting blaOXA Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Acinetobacter baumannii Clinical Strains
Dagmara Depka, Agnieszka Mikucka, Tomasz Bogiel, Mateusz Rzepka, Patryk Zawadka, Eugenia Gospodarek-Komkowska
Antibiotics.2022; 11(4): 455. CrossRef
In vitro synergistic activity of colistin and teicoplanin combination against multidrug-resistant Acinetobacter spp
Osama Mohamed Samy Mohamed Rady, Laila El-Attar, Amira Amine
The Journal of Antibiotics.2022; 75(3): 181. CrossRef
Biogenic silver nanoparticle (Bio‐AgNP) has an antibacterial effect against carbapenem‐resistant Acinetobacter baumannii with synergism and additivity when combined with polymyxin B
Suzane Olachea Allend, Marcelle Oliveira Garcia, Kamila Furtado da Cunha, Déborah Trota Farias de Albernaz, Mirian Elert da Silva, Rodrigo Yudi Ishikame, Luciano Aparecido Panagio, Gerson Nakazaro, Guilherme Fonseca Reis, Daniela Brayer Pereira, Daiane Dr
Journal of Applied Microbiology.2022; 132(2): 1036. CrossRef
RapidResa Polymyxin Acinetobacter NP® Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii
Maxime Bouvier, Mustafa Sadek, Stefano Pomponio, Fernando D’Emidio, Laurent Poirel, Patrice Nordmann
Antibiotics.2021; 10(5): 558. CrossRef
Rapid detection of carbapenemase-producing Pseudomonas spp. using the NitroSpeed-Carba NP test
Mustafa Sadek, Laurent Poirel, Patrice Nordmann
Diagnostic Microbiology and Infectious Disease.2021; 99(3): 115280. CrossRef
Gain and loss of antibiotic resistant genes in multidrug resistant bacteria: One Health perspective
Misung Kim, Jaeeun Park, Mingyeong Kang, Jihye Yang, Woojun Park
Journal of Microbiology.2021; 59(6): 535. CrossRef
OXA-23 and OXA-40 producing carbapenem-resistant Acinetobacter baumannii in Central Illinois
Janak Koirala, Isha Tyagi, Lohitha Guntupalli, Sameena Koirala, Udita Chapagain, Christopher Quarshie, Sami Akram, Vidya Sundareshan, Sajan Koirala, Jerry Lawhorn, Yohei Doi, Michael Olson
Diagnostic Microbiology and Infectious Disease.2020; 97(1): 114999. CrossRef
Rapid Polymyxin/Pseudomonas NP test for rapid detection of polymyxin susceptibility/resistance in Pseudomonas aeruginosa
Mustafa Sadek, Camille Tinguely, Laurent Poirel, Patrice Nordmann
European Journal of Clinical Microbiology & Infectious Diseases.2020; 39(9): 1657. CrossRef
Stress responses linked to antimicrobial resistance in Acinetobacter species
Bora Shin, Chulwoo Park, Woojun Park
Applied Microbiology and Biotechnology.2020; 104(4): 1423. CrossRef
Carbapenemases: Transforming Acinetobacter baumannii into a Yet More Dangerous Menace
Maria Soledad Ramirez, Robert A. Bonomo, Marcelo E. Tolmasky
Biomolecules.2020; 10(5): 720. CrossRef
Pharmacokinetics, Safety, and Tolerability of Intravenous Durlobactam and Sulbactam in Subjects with Renal Impairment and Healthy Matched Control Subjects
John O’Donnell, Richard A. Preston, Grigor Mamikonyan, Emily Stone, Robin Isaacs
Antimicrobial Agents and Chemotherapy.2019;[Epub] CrossRef
Efficient Delivery of Antisense Oligonucleotides by an Amphipathic Cell-Penetrating Peptide in Acinetobacter baumannii
Zhou Chen, Dan Nie, Yue Hu, Mingkai Li, Zheng Hou, Xinggang Mao, Xiaoxing Luo, Xiaoyan Xue
Current Drug Delivery.2019; 16(8): 728. CrossRef
Restoring the activity of the antibiotic aztreonam using the polyphenol epigallocatechin gallate (EGCG) against multidrug-resistant clinical isolates of Pseudomonas aeruginosa
Jonathan W. Betts, Michael Hornsey, Paul G. Higgins, Kai Lucassen, Julia Wille, Francisco J. Salguero, Harald Seifert, Roberto M. La Ragione
Journal of Medical Microbiology .2019; 68(10): 1552. CrossRef
Antibiotic-resistant clones in Gram-negative pathogens: presence of global clones in Korea
Kwan Soo Ko
Journal of Microbiology.2019; 57(3): 195. CrossRef
Alternative fate of glyoxylate during acetate and hexadecane metabolism in Acinetobacter oleivorans DR1
Chulwoo Park, Bora Shin, Woojun Park
Scientific Reports.2019;[Epub] CrossRef
Nationwide surveillance of antimicrobial resistance among clinically important Gram-negative bacteria, with an emphasis on carbapenems and colistin: Results from the Surveillance of Multicenter Antimicrobial Resistance in Taiwan (SMART) in 2018
Yu-Lin Lee, Min-Chi Lu, Pei-Lan Shao, Po-Liang Lu, Yen-Hsu Chen, Shu-Hsing Cheng, Wen-Chien Ko, Chi-Ying Lin, Ting-Shu Wu, Muh-Yong Yen, Lih-Shinn Wang, Chang-Pan Liu, Wen-Sen Lee, Zhi-Yuan Shi, Yao-Shen Chen, Fu-Der Wang, Shu-Hui Tseng, Chao-Nan Lin, Yu-
International Journal of Antimicrobial Agents.2019; 54(3): 318. CrossRef
The use of polymyxins to treat carbapenem resistant infections in neonates and children
Reenu Thomas, Sithembiso Velaphi, Sally Ellis, A. Sarah Walker, Joseph F. Standing, Paul Heath, Mike Sharland, Daniele Dona’
Expert Opinion on Pharmacotherapy.2019; 20(4): 415. CrossRef
Plasmid-mediated mcr-1 gene in Acinetobacter baumannii and Pseudomonas aeruginosa: first report from Pakistan
Fareeha Hameed, Muhammad Asif Khan, Hafsah Muhammad, Tahir Sarwar, Hazrat Bilal, Tayyab Ur Rehman
Revista da Sociedade Brasileira de Medicina Tropical.2019;[Epub] CrossRef
Identification of factors needed by a clinical isolate of Acinetobacter baumannii to resist antibacterial compounds
Celena M. Gwin, Natalia Prakash, Nathan W. Rigel
BIOS.2019; 90(3): 149. CrossRef
A Resazurin Reduction-Based Assay for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii and Pseudomonas aeruginosa
Mathilde Lescat, Laurent Poirel, Camille Tinguely, Patrice Nordmann, Nathan A. Ledeboer
Journal of Clinical Microbiology.2019;[Epub] CrossRef
Expansion of antibacterial spectrum of xanthorrhizol against Gram-negatives in combination with PMBN and food-grade antimicrobials
Man Su Kim, Ha-Rim Kim, Haebom Kim, Soo-Keun Choi, Chang-Hwan Kim, Jae-Kwan Hwang, Seung-Hwan Park
Journal of Microbiology.2019; 57(5): 405. CrossRef
Carbapenem-resistant Acinetobacter baumannii in patients with burn injury: A systematic review and meta-analysis
William Gustavo Lima, Geisa Cristina Silva Alves, Cristina Sanches, Simone Odília Antunes Fernandes, Magna Cristina de Paiva
Burns.2019; 45(7): 1495. CrossRef
Performances of the Rapid Polymyxin Acinetobacter and Pseudomonas Tests for Colistin Susceptibility Testing
Mathilde Lescat, Laurent Poirel, Aurélie Jayol, Patrice Nordmann
Microbial Drug Resistance.2019; 25(4): 520. CrossRef
In vitro activities of ceftazidime/avibactam alone or in combination with antibiotics against multidrug-resistant Acinetobacter baumannii isolates
Emel Mataracı Kara, Mesut Yılmaz, Berna Özbek Çelik
Journal of Global Antimicrobial Resistance.2019; 17: 137. CrossRef
Zoonotic Diseases and Phytochemical Medicines for Microbial Infections in Veterinary Science: Current State and Future Perspective
Bora Shin, Woojun Park
Frontiers in Veterinary Science.2018;[Epub] CrossRef
A formidable foe: carbapenem-resistant Acinetobacter baumannii and emerging nonantibiotic therapies
Richard R. Watkins
Expert Review of Anti-infective Therapy.2018; 16(8): 591. CrossRef
Plasma and Intrapulmonary Concentrations of ETX2514 and Sulbactam following Intravenous Administration of ETX2514SUL to Healthy Adult Subjects
Keith A. Rodvold, Mark H. Gotfried, Robin D. Isaacs, John P. O'Donnell, Emily Stone
Antimicrobial Agents and Chemotherapy.2018;[Epub] CrossRef

Journal Articles

Corynebacterium defluvii sp. nov., isolated from Sewage: Qiu-Li Yu , Zheng-Fei Yan , Feng-Hua Tian , Chuan-Wen Jia , Chang-Tian Li; J. Microbiol. 2017;55(6):435-439. Published online April 20, 2017; DOI: https://doi.org/10.1007/s12275-017-6592-3

53 View
0 Download
4 Crossref

Abstract

A Gram-positive, aerobic, non-motile, rod-shapeds, cata-lase-positive, and oxidase-negative strain, designated Y49T, was isolated from sewage collected from Jilin Agricultural University, China. It grew at 20–40°C (optimum at 30°C), at pH 6.0–8.0 (optimum at 7.0) and at 0–1.0% sodium chlo-ride (optimum at 0%). The major isoprenoid quinone was menaquinone-8 (MK-8) and the polar lipids were diphos-phatidylglycerol, phosphatidylglycerol, phosphatidylmethy-lethanolamine, four unidentified lipids, and two unidenti-fied aminolipids. The peptidoglycan was meso-diaminopi-melic acid. The cell-wall sugars were galactose, arabinose, and glucose. The fatty acids were C9:0, C16:0, C16:1 ω9c, C17:1 ω9c, C18:3 ω6c (6,9,12), C18:1 ω9c, and C18:0. The DNA G+C content was 51.4 mol%. Based on the 16S rRNA gene se-quence analysis, the nearest phylogenetic neighbors of strain Y49T were Corynebacterium efficiens DSM 44549T (97.5%), Corynebacterium callunae DSM 20147T (97.2%), Coryne-bacterium deserti GIMN 1.010T (96.8%), Corynebacterium glutamicum ATCC 13032T (96.4%), and other species belong-ing to this genus (92.3–95.4%). The DNA-DNA relatedness value between strain Y49T and C. efficiens DSM 44549T, C. callunae DSM 20147T, C. deserti GIMN1.010T, and C. gluta-micum ATCC 13032T was 25.5±2.0%, 21.1±1.0%, 16.5±0.5%, and 13.5±0.9%, respectively. Based on the phylogenetic an-alysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain Y49T represents a novel species of the genus Corynebacterium, for which the name Corynebacterium defluvii sp nov. is proposed. The type strain is Y49T (= KCTC 39731T =CGMCC 1.15506T).

Citations

Citations to this article as recorded by

Corynebacterium kalidii sp. nov, an endophyte from a shoot of the halophyte Kalidium cuspidatum
Jia-Yi Feng, Lian Xu, Shu-Kun Tang, Ji-Quan Sun
Archives of Microbiology.2022;[Epub] CrossRef
Corynebacterium zhongnanshanii sp. nov. isolated from trachea of Marmota himalayana, Corynebacterium lujinxingii sp. nov. and Corynebacterium wankanglinii sp. nov. from human faeces
Gui Zhang, Jing Yang, Xin-He Lai, Dong Jin, Shan Lu, Zhihong Ren, Tian Qin, Ji Pu, Yajun Ge, Yanpeng Cheng, Caixin Yang, Xianglian Lv, Yifan Jiao, Ying Huang, Jianguo Xu
International Journal of Systematic and Evolutionary Microbiology .2021;[Epub] CrossRef
Corynebacterium glutamicum Mechanosensing: From Osmoregulation to L-Glutamate Secretion for the Avian Microbiota-Gut-Brain Axis
Yoshitaka Nakayama
Microorganisms.2021; 9(1): 201. CrossRef
Taxonomic status of Corynebacterium diphtheriae biovar Belfanti and proposal of Corynebacterium belfantii sp. nov
Melody Dazas, Edgar Badell, Annick Carmi-Leroy, Alexis Criscuolo, Sylvain Brisse
International Journal of Systematic and Evolutionary Microbiology.2018; 68(12): 3826. CrossRef

Deinococcus rubellus sp. nov., bacteria isolated from the muscle of antarctic fish: Seok-Gwan Choi , Seon Hwa Jeon , Jae-Bong Lee , Eun Sun Joo , Sangyong Lim , Hee-Young Jung , Myung Kyum Kim; J. Microbiol. 2016;54(12):796-801. Published online November 26, 2016; DOI: https://doi.org/10.1007/s12275-016-6390-3

53 View
0 Download
1 Crossref

Abstract

Two new bacterial strains designated as Ant6T and Ant18 were isolated from the muscle of a fish which had been caught in the Antarctic Ocean. Both strains are Gram-stain-positive, catalase positive, oxidase negative, aerobic, and coccoid bacteria. Phylogenetic analysis based on the 16S rRNA gene sequences of strains Ant6T and Ant18 revealed that the strains Ant6T and Ant18 belong to the genus Deinococcus in the family Deinococcaceae in the class Deinococci. The highest degrees of sequence similarities of strains Ant6T and Ant18 were found with Deinococcus alpinitundrae LMG 24283T by 96.4% and 96.8%, respectively. Strain Ant6T exhibited a high level of DNA- DNA hybridization values with strain Ant18 (82 ± 0.6%). Chemotaxonomic data revealed that the predominant fatty acids were C17􍾙:􍾙0 cyclo, 16:0, and feature 3 (C16:1 ω6c/ω7c) for both strains. A complex polar lipid profile consisted of major amounts of unknown phosphoglycolipids (PGL) and unknown aminophospholipid (APL). Based on the phylogenetic, phenotypic, and chemotaxonomic data, strains Ant6T (=KEMB 9004-169T =JCM 31434T) and Ant18 (=KEMB 9004- 170) should be classified as a new species, for which the name Deinococcus rubellus sp. nov. is proposed.

Citations

Citations to this article as recorded by

Complete genome sequence of Deinococcus rubellus Ant6 isolated from the fish muscle in the Antarctic Ocean
Surajit De Mandal, Sathiyaraj Srinivasan, Junhyun Jeon
Frontiers in Bioengineering and Biotechnology.2023;[Epub] CrossRef

Research Support, Non-U.S. Gov't

Adjuvant Efficacy of mOMV against Avian Influenza Virus Infection in Mice: Byeong-Jae Lee , Sang-Ho Lee , Min-Suk Song , Philippe Noriel Q. Pascua , Hyeok-il Kwon , Su-Jin Park , Eun-Ha Kim , Arun Decano , Se Mi Kim , Gyo Jin Lim , Doo-Jin Kim , Kyu-Tae Chang , Sang-Hyun Kim , Young Ki Choi; J. Microbiol. 2013;51(5):682-688. Published online October 31, 2013; DOI: https://doi.org/10.1007/s12275-013-3411-3

27 View
0 Download
1 Scopus

Abstract: Highly pathogenic avian influenza H5N1 viruses are found chiefly in birds and have caused severe disease and death in infected humans. Development of influenza vaccines capable of inducing heterosubtypic immunity against a broad range of influenza viruses is the best option for the preparedness, since vaccination remains the principal method in controlling influenza viral infections. Here, a mOMV-adjuvanted recombinant H5N2 (rH5N2) whole virus antigen vaccine with A/Environment/Korea/W149/06(H5N1)-derived H5 HA and A/Chicken/Korea/ma116/04(H9N2)-derived N2 NA in the backbone of A/Puerto Rico/8/34(H1N1) was prepared and generated by reverse genetics. Groups of mice were vaccinated by a prime-boost regime with the rH5N2 vaccine (1.75 μg of HA with/without 10 μg mOMV or aluminum hydroxide adjuvant for comparison). At two weeks post-immunizations, vaccinated mice were challenged with lethal doses of 103.5 EID50/ml of H5N1 or H9N2 avian influenza viruses, and were monitored for 15 days. Both mOMV- and alum-adjuvant vaccine groups had high survival rates after H5N1 infection and low levels of body weight changes compared to control groups. Interestingly, the mOMV-adjuvanted group induced better cross-reactive antibody responses serologically and promoted cross-protectivity against H5N1 and H9N2 virus challenges. Our results suggest that mOMV could be used as a vaccine adjuvant in the development of effective vaccines used to control influenza A virus transmission.

Research Support, U.S. Gov't, Non-P.H.S.

Combined Effect of Microbial and Chemical Control Agents on Subterranean Termites: Maureen S. Wright , Alan R. Lax; J. Microbiol. 2013;51(5):578-583. Published online September 14, 2013; DOI: https://doi.org/10.1007/s12275-013-2628-5

43 View
0 Download
5 Crossref

Abstract

Termite mortality was measured when fungi were combined with bacteria or a chemical termiticide to determine whether a synergistic effect occurred. The fungus Beauveria bassiana was combined with the non-repellant chemical termiticide imidacloprid. Of the three B. bassiana strains tested one, B. bassiana ATCC 90519, was sufficiently pathogenic on its own that the advantage of a supplementary chemical treatment was marginal. The mortality caused by another fungal strain, B. bassiana ATCC 26037, was improved in combination with imidacloprid at both of the tested chemical concentrations over the first 14 days. The remaining fungal strain, B. bassiana ATCC 90518, demonstrated an overall mortality rate in combination with imidacloprid of 82.5%, versus a rate of 65.0% for the fungus alone. The fungus Isaria fumosorosea (Ifr) was combined with the bacterium Bacillus thuringiensis (Bt). On day 5, Ifr, Bt, and the combined treatment at a 106 spores or cells/ml dosage caused 8.8%, 22.5%, and 15.0% mortality, respectively. The Bt and combined mortality rates are not significantly different. Control mortality on day 5 was 5.0%. On day 13 the combined 106 treatment mortality rate was 91.3%, which was significantly higher than all other treatments: control at 17.5%, Ifr at 36.3% and Bt at 35.0%. When Ifr and Bt were applied at a 109 spores or cells/ml dosage, Ifr alone caused a mortality rate of 97.5% as early as day 5. The combination with Bt could not significantly increase the effectiveness of this dosage. These data demonstrate the potential for synergistic effects of fungal and chemical treatment methods, thereby broadening the use of microbial control agents and reducing the quantity of chemical agents necessary to effect control.

Citations

Citations to this article as recorded by

RNAi-mediated silencing of transferrin promotes entomopathogens lethality in Odontotermes formosanus (Shiraki)
Zhiqiang Wang, Yujingyun Zhou, Fang Tang
Pesticide Biochemistry and Physiology.2024; 205: 106149. CrossRef
Termite management by entomopathogenic fungi: Recent advances and future prospects
Ali Hassan, Zhiqiang Li, Xuguo Zhou, Jianchu Mo, Qiuying Huang
Current Research in Biotechnology.2024; 7: 100183. CrossRef
Termites and Chinese agricultural system: applications and advances in integrated termite management and chemical control
Farhan Ahmad, Hatem Fouad, Shi‐You Liang, Yin Hu, Jian‐Chu Mo
Insect Science.2021; 28(1): 2. CrossRef
Influence of Zwitterionic Buffer Effects with Thermal Modification Treatments of Wood on Symbiotic Protists in Reticulitermes grassei Clément
Sónia Duarte, Lina Nunes, Davor Kržišnik, Miha Humar, Dennis Jones
Insects.2021; 12(2): 139. CrossRef
The potential ofIsariaspp. as a bioinsecticide for the biological control ofNasutitermes corniger
Rosineide da Silva Lopes, Geiziquele de Lima, Maria Tereza dos Santos Correia, Antonio Félix da Costa, Elza Áurea de Luna Alves Lima, Vera Lúcia de Menezes Lima
Biocontrol Science and Technology.2017; 27(9): 1038. CrossRef

Research Support, Non-U.S. Gov't

Saccharomyces cerevisiae Cmr1 Protein Preferentially Binds to UV-Damaged DNA In Vitro: Do-Hee Choi , Sung-Hun Kwon , Joon-Ho Kim , Sung-Ho Bae; J. Microbiol. 2012;50(1):112-118. Published online February 27, 2012; DOI: https://doi.org/10.1007/s12275-012-1597-4

42 View
0 Download
13 Scopus

Abstract: DNA metabolic processes such as DNA replication, recombination, and repair are fundamentally important for the maintenance of genome integrity and cell viability. Although a large number of proteins involved in these pathways have been extensively studied, many proteins still remain to be identified. In this study, we isolated DNA-binding proteins from Saccharomyces cerevisiae using DNA-cellulose columns. By analyzing the proteins using mass spectrometry, an uncharacterized protein, Cmr1/YDL156W, was identified. Cmr1 showed sequence homology to human Damaged-DNA binding protein 2 in its C-terminal WD40 repeats. Consistent with this finding, the purified recombinant Cmr1 protein was found to be intrinsically associated with DNA-binding activity and exhibited higher affinity to UV-damaged DNA substrates. Chromatin isolation experiments revealed that Cmr1 localized in both the chromatin and supernatant fractions, and the level of Cmr1 in the chromatin fraction increased when yeast cells were irradiated with UV. These
results
suggest that Cmr1 may be involved in DNA-damage responses in yeast.

First
Prev
Page of 2
Next
Last

Search