Journal Article
- ZntR positively regulates T6SS4 expression in Yersinia pseudotuberculosis
-
Tietao Wang , Keqi Chen , Fen Gao , Yiwen Kang , Muhammad Tausif Chaudhry , Zhuo Wang , Yao Wang , Xihui Shen
-
J. Microbiol. 2017;55(6):448-456. Published online March 10, 2017
-
DOI: https://doi.org/10.1007/s12275-017-6540-2
-
-
52
View
-
0
Download
-
19
Crossref
-
Abstract
-
The type VI secretion system (T6SS) is a widespread and versatile protein secretion system found in most Gram- negative bacteria. Studies of T6SS have mainly focused on its role in virulence toward host cells and inter-bacterial inter-actions, but studies have also shown that T6SS4 in Yersinia pseudotuberculosis participates in the acquisition of zinc ions to alleviate the accumulation of hydroxyl radicals induced by multiple stressors. Here, by comparing the gene expression patterns of wild-type and zntR mutant Y. pseudotubercu-losis cells using RNA-seq analysis, T6SS4 and 17 other bio-logical processes were found to be regulated by ZntR. T6SS4 was positively regulated by ZntR in Y. pseudotuberculosis, and further investigation demonstrated that ZntR regulates T6SS4 by directly binding to its promoter region. T6SS4 ex-pression is regulated by zinc via ZntR, which maintains in-tracellular zinc homeostasis and controls the concentration of reactive oxygen species to prevent bacterial death under oxidative stress. This study provides new insights into the regulation of T6SS4 by a zinc-dependent transcriptional regu-lator, and it provides a foundation for further investigation of the mechanism of zinc transport by T6SS.
-
Citations
Citations to this article as recorded by

- Two-Component Signaling System RegAB Represses Pseudomonas syringae pv. actinidiae T3SS by Directly Binding to the promoter of hrpRS1
Mengsi Zhang, Mingming Yang, Xiaoxue Zhang, Shuying Li, Shuaiwu Wang, Alex Muremi Fulano, Yongting Meng, Xihui Shen, Li-li Huang, Yao Wang
Journal of Integrative Agriculture.2024;[Epub] CrossRef - Genome-wide phenotypic profiling of transcription factors and identification of novel targets to control the virulence of Vibrio vulnificus
Dayoung Sung, Garam Choi, Minji Ahn, Hokyung Byun, Tae Young Kim, Hojun Lee, Zee-Won Lee, Ji Yong Park, Young Hyun Jung, Ho Jae Han, Sang Ho Choi
Nucleic Acids Research.2024;[Epub] CrossRef - Pb2+ biosorption by Serratia marcescens CCMA 1010 and its relation with zntR gene expression and ZntA efflux pump regulation
Jorge Dias Carlier, Gustavo Magno dos Reis Ferreira, Rosane Freitas Schwan, Cristina Ferreira da Silva, Maria Clara Costa
Environmental Advances.2024; 15: 100479. CrossRef - OxyR-regulated T6SS functions in coordination with siderophore to resist oxidative stress
Changfu Li, Zhiyan Wei, Xinquan He, Haiyang He, Yuqi Liu, Yuxin Zuo, He Xiao, Yao Wang, Xihui Shen, Lingfang Zhu, Olaya Rendueles
Microbiology Spectrum.2024;[Epub] CrossRef - A σE-mediated temperature gauge orchestrates type VI secretion system, biofilm formation and cell invasion in pathogen Pseudomonas plecoglossicida
Yibei Zhang, Yuping Huang, Haoyuan Ding, Jiabao Ma, Xinyu Tong, Yuanxing Zhang, Zhen Tao, Qiyao Wang
Microbiological Research.2023; 266: 127220. CrossRef - Impact of lead (Pb2+) on the growth and biological activity of Serratia marcescens selected for wastewater treatment and identification of its zntR gene—a metal efflux regulator
Gustavo Magno dos Reis Ferreira, Josiane Ferreira Pires, Luciana Silva Ribeiro, Jorge Dias Carlier, Maria Clara Costa, Rosane Freitas Schwan, Cristina Ferreira Silva
World Journal of Microbiology and Biotechnology.2023;[Epub] CrossRef -
MlrA, a MerR family regulator in
Vibrio cholerae
, senses the anaerobic signal in the small intestine of the host to promote bacterial intestinal colonization
Jialin Wu, Yutao Liu, Wendi Li, Fan Li, Ruiying Liu, Hao Sun, Jingliang Qin, Xiaohui Feng, Di Huang, Bin Liu
Gut Microbes.2022;[Epub] CrossRef - Nutritional immunity: the battle for nutrient metals at the host–pathogen interface
Caitlin C. Murdoch, Eric P. Skaar
Nature Reviews Microbiology.2022; 20(11): 657. CrossRef - The transcriptional regulator Zur regulates the expression of ZnuABC and T6SS4 in response to stresses in Yersinia pseudotuberculosis
Ran Cai, Fen Gao, Junfeng Pan, Xinwei Hao, Zonglan Yu, Yichen Qu, Jialin Li, Dandan Wang, Yao Wang, Xihui Shen, Xingyu Liu, Yantao Yang
Microbiological Research.2021; 249: 126787. CrossRef - T6SS Mediated Stress Responses for Bacterial Environmental Survival and Host Adaptation
Kai-Wei Yu, Peng Xue, Yang Fu, Liang Yang
International Journal of Molecular Sciences.2021; 22(2): 478. CrossRef -
Yersiniabactin contributes to overcoming zinc restriction during
Yersinia pestis
infection of mammalian and insect hosts
Sarah L. Price, Viveka Vadyvaloo, Jennifer K. DeMarco, Amanda Brady, Phoenix A. Gray, Thomas E. Kehl-Fie, Sylvie Garneau-Tsodikova, Robert D. Perry, Matthew B. Lawrenz
Proceedings of the National Academy of Sciences.2021;[Epub] CrossRef - Roles of Type VI Secretion System in Transport of Metal Ions
Xiaobing Yang, Hai Liu, Yanxiong Zhang, Xihui Shen
Frontiers in Microbiology.2021;[Epub] CrossRef - Beyond dueling: roles of the type VI secretion system in microbiome modulation, pathogenesis and stress resistance
Jinshui Lin, Lei Xu, Jianshe Yang, Zhuo Wang, Xihui Shen
Stress Biology.2021;[Epub] CrossRef - Coordinated regulation of anthranilate metabolism and bacterial virulence by the GntR family regulator MpaR in Pseudomonas aeruginosa
Tietao Wang, Yihang Qi, Zhihan Wang, Jingru Zhao, Linxuan Ji, Jun Li, Zhao Cai, Liang Yang, Min Wu, Haihua Liang
Molecular Microbiology.2020; 114(5): 857. CrossRef - RovC - a novel type of hexameric transcriptional activator promoting type VI secretion gene expression
Vanessa Knittel, Pooja Sadana, Stephanie Seekircher, Anne-Sophie Stolle, Britta Körner, Marcel Volk, Cy M. Jeffries, Dmitri I. Svergun, Ann Kathrin Heroven, Andrea Scrima, Petra Dersch, Joan Mecsas
PLOS Pathogens.2020; 16(9): e1008552. CrossRef - The type VI secretion system protein AsaA in Acinetobacter baumannii is a periplasmic protein physically interacting with TssM and required for T6SS assembly
Lei Li, Yi-Nuo Wang, Hong-Bing Jia, Ping Wang, Jun-Fang Dong, Juan Deng, Feng-Min Lu, Qing-Hua Zou
Scientific Reports.2019;[Epub] CrossRef - Confirmed and Potential Roles of Bacterial T6SSs in the Intestinal Ecosystem
Can Chen, Xiaobing Yang, Xihui Shen
Frontiers in Microbiology.2019;[Epub] CrossRef - The stringent response factor, RelA, positively regulates T6SS4 expression through the RovM/RovA pathway in Yersinia pseudotuberculosis
Xiaobing Yang, Yunhong Song, Qingyun Dai, Hongyun Zhang, Li Song, Zhuo Wang, Junfeng Pan, Yao Wang
Microbiological Research.2019; 220: 32. CrossRef - Type VI Secretion Systems Present New Insights on Pathogenic Yersinia
Xiaobing Yang, Junfeng Pan, Yao Wang, Xihui Shen
Frontiers in Cellular and Infection Microbiology.2018;[Epub] CrossRef
Research Support, Non-U.S. Gov't
- Roles of RpoS in Yersinia pseudotuberculosis stress survival, motility, biofilm formation and type VI secretion system expression
-
Jingyuan Guan , Xiao Xiao , Shengjuan Xu , Fen Gao , Jianbo Wang , Tietao Wang , Yunhong Song , Junfeng Pan , Xihui Shen , Yao Wang
-
J. Microbiol. 2015;53(9):633-642. Published online August 27, 2015
-
DOI: https://doi.org/10.1007/s12275-015-0099-6
-
-
46
View
-
0
Download
-
44
Crossref
-
Abstract
-
RpoS (σS), the stationary phase/stress σ factor, controls the
expression of a large number of genes involved in cellular
responses to a variety of stresses. However, the role of RpoS
appears to differ in different bacteria. While RpoS is an important
regulator of flagellum biosynthesis, it is associated
with biofilm development in Edwardsiella tarda. Biofilms
are dense communities formed by bacteria and are important
for microbe survival under unfavorable conditions. The type
VI secretion system (T6SS) discovered recently is reportedly
associated with several phenotypes, ranging from biofilm
formation to stress sensing. For example, Vibrio anguillarum
T6SS was proposed to serve as a sensor for extracytoplasmic
signals and modulates RpoS expression and stress response.
In this study, we investigated the physiological roles of RpoS
in Yersinia pseudotuberculosis, including bacterial survival
under stress conditions, flagella formation, biofilm development
and T6SS expression. We found that RpoS is important
in resistance to multiple stressors–including H2O2, acid,
osmotic and heat shock–in Y. pseudotuberculosis. In addition,
our study showed that RpoS not only modulates the expression
of T6SS but also regulates flagellum formation by
positively controlling the flagellar master regulatory gene
flhDC, and affects the formation of biofilm on Caenorhabditis
elegans by regulating the synthesis of exopolysaccharides.
Taken together, these results show that RpoS plays a central
role in cell fitness under several adverse conditions in Y.
pseudotuberculosis.
-
Citations
Citations to this article as recorded by

- Biofilm-mediated infections by multidrug-resistant microbes: a comprehensive exploration and forward perspectives
Mai M. Zafer, Gamal A. Mohamed, Sabrin R. M. Ibrahim, Soumya Ghosh, Charné Bornman, Mahmoud A. Elfaky
Archives of Microbiology.2024;[Epub] CrossRef - The role of the type VI secretion system in the stress resistance of plant-associated bacteria
Rui Yin, Juanli Cheng, Jinshui Lin
Stress Biology.2024;[Epub] CrossRef - OxyR-regulated T6SS functions in coordination with siderophore to resist oxidative stress
Changfu Li, Zhiyan Wei, Xinquan He, Haiyang He, Yuqi Liu, Yuxin Zuo, He Xiao, Yao Wang, Xihui Shen, Lingfang Zhu, Olaya Rendueles
Microbiology Spectrum.2024;[Epub] CrossRef - Genome sequence of Leclercia adecarboxylata QDSM01 with multiple plant growth promoting properties
Wenjing Chen, Zhigang Wang, Weihui Xu, Yunlong Hu
Plant Growth Regulation.2024; 102(2): 445. CrossRef - Function and Global Regulation of Type III Secretion System and Flagella in Entomopathogenic Nematode Symbiotic Bacteria
Xiyin Huang, Chen Li, Ke Zhang, Kunyan Li, Jiajie Xie, Yuyuan Peng, Meifang Quan, Yunjun Sun, Yibo Hu, Liqiu Xia, Shengbiao Hu
International Journal of Molecular Sciences.2024; 25(14): 7579. CrossRef - RpoS sigma factor mediates adaptation and virulence in Vibrio mimicus
Ziyan Jiang, Anting Chen, Zhen Chen, Jingwen Xu, Xiaojian Gao, Qun Jiang, Xiaojun Zhang
International Journal of Biological Macromolecules.2024; 279: 135307. CrossRef - The Missing Pieces: The Role of Secretion Systems in Campylobacter jejuni Virulence
Amber D. Gabbert, Jennifer L. Mydosh, Prabhat K. Talukdar, Lisa M. Gloss, Jason E. McDermott, Kerry K. Cooper, Geremy C. Clair, Michael E. Konkel
Biomolecules.2023; 13(1): 135. CrossRef - Transcriptional organization and regulation of the Pseudomonas putida K1 type VI secretion system gene cluster
Patricia Bernal, Cristina Civantos, Daniel Pacheco-Sánchez, José M. Quesada, Alain Filloux, María A. Llamas
Microbiology
.2023;[Epub] CrossRef - Regulation of type VI secretion systems at the transcriptional, posttranscriptional and posttranslational level
Julia Takuno Hespanhol, Luize Nóbrega-Silva, Ethel Bayer-Santos
Microbiology
.2023;[Epub] CrossRef - RpoS-Regulated Genes and Phenotypes in the Phytopathogenic Bacterium Pectobacterium atrosepticum
Olga Petrova, Elizaveta Semenova, Olga Parfirova, Ivan Tsers, Natalia Gogoleva, Yuri Gogolev, Yevgeny Nikolaichik, Vladimir Gorshkov
International Journal of Molecular Sciences.2023; 24(24): 17348. CrossRef - DegS protease regulates the motility, chemotaxis, and colonization of Vibrio cholerae
Mei Zou, Kaiying Wang, Jiajun Zhao, Huifang Lu, Hui Yang, Meirong Huang, Lu Wang, Guangli Wang, Jian Huang, Xun Min
Frontiers in Microbiology.2023;[Epub] CrossRef - Roles of Hcp2, a Hallmark of T6SS2 in Motility, Adhesive Capacity, and Pathogenicity of Vibrio alginolyticus
Shuilong Wu, Jufen Tang, Bei Wang, Jia Cai, Jichang Jian
Microorganisms.2023; 11(12): 2893. CrossRef - The GacS/A-Rsm Pathway Positively Regulates Motility and Flagella Synthesis in Azotobacter vinelandii
Liliana López-Pliego, Norarizbeth Lara-Flores, Dalia Molina-Romero, Gabriela May-Compañ, Ricardo Carreño-López, Cinthia E. Núñez, Miguel Castañeda
Current Microbiology.2022;[Epub] CrossRef - Sigma factor RpoS positively affects the spoilage activity of Shewanella baltica and negatively regulates its adhesion effect
Caili Zhang, Jiaqi Chen, Xiaoming Pan, Haimei Liu, Yanlong Liu
Frontiers in Microbiology.2022;[Epub] CrossRef - Exploring the Function of Quorum Sensing Regulated Biofilms in Biological Wastewater Treatment: A Review
Sania Sahreen, Hamid Mukhtar, Kálmán Imre, Adriana Morar, Viorel Herman, Sundas Sharif
International Journal of Molecular Sciences.2022; 23(17): 9751. CrossRef - A c-di-GMP Signaling Cascade Controls Motility, Biofilm Formation, and Virulence in Burkholderia thailandensis
Zhuo Wang, Xiaorong Xie, Daohan Shang, Laigong Xie, Yueyue Hua, Li Song, Yantao Yang, Yao Wang, Xihui Shen, Lei Zhang, Gladys Alexandre
Applied and Environmental Microbiology.2022;[Epub] CrossRef - Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of pathogenic Enterobacter cloacae from Macrobrachium rosenbergii
Xiaojian Gao, Qieqi Qian, Yujie Zhu, Zhen Chen, Jingwen Xu, Wenjing Xu, Qun Jiang, Jun Wang, Xiaojun Zhang
Frontiers in Microbiology.2022;[Epub] CrossRef - Cpx-signalling facilitates Hms-dependent biofilm formation by Yersinia pseudotuberculosis
Dharmender K. Gahlot, Sun N. Wai, David L. Erickson, Matthew S. Francis
npj Biofilms and Microbiomes.2022;[Epub] CrossRef - MomL inhibits bacterial antibiotic resistance through the starvation stringent response pathway
Qin Dou, Jin Yuan, Rilei Yu, Jiahui Yang, Jiayi Wang, Yuxiang Zhu, Jing Zhong, Hongan Long, Zhiqing Liu, Xianghong Wang, Yuying Li, Yichen Xiao, Jiazhen Liang, Xiao‐Hua Zhang, Yan Wang
mLife.2022; 1(4): 428. CrossRef - Bacterial Stress Responses as Potential Targets in Overcoming Antibiotic Resistance
Jirapat Dawan, Juhee Ahn
Microorganisms.2022; 10(7): 1385. CrossRef - The GntR-like transcriptional regulator HutC involved in motility, biofilm-forming ability, and virulence in Vibrio parahaemolyticus
Yangyang Li, Weidong Sun, Quan Wang, Ying Yu, Ying Wan, Kai Zhou, Rong Guo, Xiangan Han, Zhaoguo Chen, Weihuan Fang, Wei Jiang
Microbial Pathogenesis.2022; 167: 105546. CrossRef - The transcriptional regulator Zur regulates the expression of ZnuABC and T6SS4 in response to stresses in Yersinia pseudotuberculosis
Ran Cai, Fen Gao, Junfeng Pan, Xinwei Hao, Zonglan Yu, Yichen Qu, Jialin Li, Dandan Wang, Yao Wang, Xihui Shen, Xingyu Liu, Yantao Yang
Microbiological Research.2021; 249: 126787. CrossRef - RpoS Activates the Prodigionsin Production by Activating the Transcription of the RpoS-Dependent Pig Gene Cluster in Serratia marcescens FS14
Baoling Yang, Fenglian Chu, Haixia Li, Weiwu Wang, Tingting Ran, Dongqing Xu
Indian Journal of Microbiology.2021; 61(3): 355. CrossRef - Beyond dueling: roles of the type VI secretion system in microbiome modulation, pathogenesis and stress resistance
Jinshui Lin, Lei Xu, Jianshe Yang, Zhuo Wang, Xihui Shen
Stress Biology.2021;[Epub] CrossRef -
Role of RpoS in stress resistance, biofilm formation and quorum sensing of
Shewanella baltica
C. Zhang, C. Wang, A.‐N. Jatt, H. Liu, Y. Liu
Letters in Applied Microbiology.2021; 72(3): 307. CrossRef - Roles of Type VI Secretion System in Transport of Metal Ions
Xiaobing Yang, Hai Liu, Yanxiong Zhang, Xihui Shen
Frontiers in Microbiology.2021;[Epub] CrossRef - Microbial biofilm ecology, in silico study of quorum sensing receptor-ligand interactions and biofilm mediated bioremediation
Biji Balan, Amit S. Dhaulaniya, Diksha A. Varma, Kushneet K. Sodhi, Mohit Kumar, Manisha Tiwari, Dileep Kumar Singh
Archives of Microbiology.2021; 203(1): 13. CrossRef - Baicalin, a natural antimicrobial and anti-biofilm agent
Mahdi Asghari Ozma, Ehsaneh Khodadadi, Farzaneh Pakdel, Fadhil S. Kamounah, Mehdi Yousefi, Bahman Yousefi, Mohammad Asgharzadeh, Khudaverdi Ganbarov, Hossein Samadi Kafil
Journal of Herbal Medicine.2021; 27: 100432. CrossRef - RovC - a novel type of hexameric transcriptional activator promoting type VI secretion gene expression
Vanessa Knittel, Pooja Sadana, Stephanie Seekircher, Anne-Sophie Stolle, Britta Körner, Marcel Volk, Cy M. Jeffries, Dmitri I. Svergun, Ann Kathrin Heroven, Andrea Scrima, Petra Dersch, Joan Mecsas
PLOS Pathogens.2020; 16(9): e1008552. CrossRef - Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s
Ping Wang, Jun-Fang Dong, Ren-Qing Li, Lei Li, Qing-Hua Zou
Virulence.2020; 11(1): 1716. CrossRef -
Differential Gene Expression Patterns of
Yersinia pestis
and
Yersinia pseudotuberculosis
during Infection and Biofilm Formation in the Flea Digestive Tract
Iman Chouikha, Daniel E. Sturdevant, Clayton Jarrett, Yi-Cheng Sun, B. Joseph Hinnebusch, Seth Bordenstein
mSystems.2019;[Epub] CrossRef - Confirmed and Potential Roles of Bacterial T6SSs in the Intestinal Ecosystem
Can Chen, Xiaobing Yang, Xihui Shen
Frontiers in Microbiology.2019;[Epub] CrossRef - Combined Transcriptome and Proteome Analysis of RpoS Regulon Reveals Its Role in Spoilage Potential of Pseudomonas fluorescens
Xiaoxiang Liu, Jun Xu, Junli Zhu, Peng Du, Aihua Sun
Frontiers in Microbiology.2019;[Epub] CrossRef - The type VI secretion system protein AsaA in Acinetobacter baumannii is a periplasmic protein physically interacting with TssM and required for T6SS assembly
Lei Li, Yi-Nuo Wang, Hong-Bing Jia, Ping Wang, Jun-Fang Dong, Juan Deng, Feng-Min Lu, Qing-Hua Zou
Scientific Reports.2019;[Epub] CrossRef - Anti-bacterial activity of baicalin against APEC through inhibition of quorum sensing and inflammatory responses
Lu-Yuan Peng, Meng Yuan, Zong-Mei Wu, Ke Song, Chun-Lei Zhang, Qiang An, Fang Xia, Jia-Lin Yu, Peng-Fei Yi, Ben-Dong Fu, Hai-Qing Shen
Scientific Reports.2019;[Epub] CrossRef - The stringent response factor, RelA, positively regulates T6SS4 expression through the RovM/RovA pathway in Yersinia pseudotuberculosis
Xiaobing Yang, Yunhong Song, Qingyun Dai, Hongyun Zhang, Li Song, Zhuo Wang, Junfeng Pan, Yao Wang
Microbiological Research.2019; 220: 32. CrossRef - Xanthomonas citri T6SS mediates resistance to Dictyostelium predation and is regulated by an ECF σ factor and cognate Ser/Thr kinase
Ethel Bayer‐Santos, Lídia dos Passos Lima, Lucas de Moraes Ceseti, Camila Yuri Ratagami, Eliane Silva de Santana, Aline Maria da Silva, Chuck Shaker Farah, Cristina Elisa Alvarez‐Martinez
Environmental Microbiology.2018; 20(4): 1562. CrossRef - Role of RpoS in stress resistance, quorum sensing and spoilage potential of Pseudomonas fluorescens
Xiaoxiang Liu, Lei Ji, Xu Wang, Jianrong Li, Junli Zhu, Aihua Sun
International Journal of Food Microbiology.2018; 270: 31. CrossRef - Type VI Secretion Systems Present New Insights on Pathogenic Yersinia
Xiaobing Yang, Junfeng Pan, Yao Wang, Xihui Shen
Frontiers in Cellular and Infection Microbiology.2018;[Epub] CrossRef - The alternative sigma factor RpoQ regulates colony morphology, biofilm formation and motility in the fish pathogen Aliivibrio salmonicida
Miriam Khider, Nils Peder Willassen, Hilde Hansen
BMC Microbiology.2018;[Epub] CrossRef -
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in
Pseudomonas aeruginosa
Luke P. Allsopp, Thomas E. Wood, Sophie A. Howard, Federica Maggiorelli, Laura M. Nolan, Sarah Wettstadt, Alain Filloux
Proceedings of the National Academy of Sciences.2017; 114(29): 7707. CrossRef - A starvation-induced regulator, RovM, acts as a switch for planktonic/biofilm state transition in Yersinia pseudotuberculosis
Ruoxi Zhao, Yunhong Song, Qingyun Dai, Yiwen Kang, Junfeng Pan, Lingfang Zhu, Lei Zhang, Yao Wang, Xihui Shen
Scientific Reports.2017;[Epub] CrossRef - ZntR positively regulates T6SS4 expression in Yersinia pseudotuberculosis
Tietao Wang, Keqi Chen, Fen Gao, Yiwen Kang, Muhammad Tausif Chaudhry, Zhuo Wang, Yao Wang, Xihui Shen
Journal of Microbiology.2017; 55(6): 448. CrossRef - Transcriptomic and Phenotypic Analysis Reveals New Functions for the Tat Pathway in Yersinia pseudotuberculosis
Ummehan Avican, Michael Beckstette, Ann Kathrin Heroven, Moa Lavander, Petra Dersch, Åke Forsberg, P. J. Christie
Journal of Bacteriology.2016; 198(20): 2876. CrossRef