Skip Navigation
Skip to contents

Journal of Microbiology : Journal of Microbiology

OPEN ACCESS
SEARCH
Search
Advanced Search
mobile menu button

Articles

Page Path
HOME > J. Microbiol > Volume 53(9); 2015 > Article
Research Support, Non-U.S. Gov't
Roles of RpoS in Yersinia pseudotuberculosis stress survival, motility, biofilm formation and type VI secretion system expression
Jingyuan Guan 1,2, Xiao Xiao 3, Shengjuan Xu 1,2, Fen Gao 1,3, Jianbo Wang 1,2, Tietao Wang 3, Yunhong Song 3, Junfeng Pan 1,2, Xihui Shen 1,3, Yao Wang 1,2
Journal of Microbiology 2015;53(9):633-642.
DOI: https://doi.org/10.1007/s12275-015-0099-6
Published online: August 27, 2015
1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China, 2Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China, 3Department of Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China
Corresponding author:  Xihui Shen , Tel: +86-29-87081062, 
Yao Wang , Tel: +86-29-87081062, 
Received: 26 February 2015   • Revised: 16 July 2015   • Accepted: 22 July 2015
prev next
  • 245 Views
  • 0 Download
  • 46 Crossref
  • 50 Scopus
Full Article

Download PDF Download PDF

Abstract

RpoS (σS), the stationary phase/stress σ factor, controls the expression of a large number of genes involved in cellular responses to a variety of stresses. However, the role of RpoS appears to differ in different bacteria. While RpoS is an important regulator of flagellum biosynthesis, it is associated with biofilm development in Edwardsiella tarda. Biofilms are dense communities formed by bacteria and are important for microbe survival under unfavorable conditions. The type VI secretion system (T6SS) discovered recently is reportedly associated with several phenotypes, ranging from biofilm formation to stress sensing. For example, Vibrio anguillarum T6SS was proposed to serve as a sensor for extracytoplasmic signals and modulates RpoS expression and stress response. In this study, we investigated the physiological roles of RpoS in Yersinia pseudotuberculosis, including bacterial survival under stress conditions, flagella formation, biofilm development and T6SS expression. We found that RpoS is important in resistance to multiple stressors–including H2O2, acid, osmotic and heat shock–in Y. pseudotuberculosis. In addition, our study showed that RpoS not only modulates the expression of T6SS but also regulates flagellum formation by positively controlling the flagellar master regulatory gene flhDC, and affects the formation of biofilm on Caenorhabditis elegans by regulating the synthesis of exopolysaccharides. Taken together, these results show that RpoS plays a central role in cell fitness under several adverse conditions in Y. pseudotuberculosis.

  • Cite this Article
    Cite this Article
    export Copy Download
    Close
    Download Citation
    Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.
    Format:
    • RIS — For EndNote, ProCite, RefWorks, and most other reference management software
    • BibTeX — For JabRef, BibDesk, and other BibTeX-specific software
    Include:
    • Citation for the content below
    Roles of RpoS in Yersinia pseudotuberculosis stress survival, motility, biofilm formation and type VI secretion system expression
    J. Microbiol. 2015;53(9):633-642.   Published online August 27, 2015
    DOI: https://doi.org/10.1007/s12275-015-0099-6
    Close
Related articles
Journal of Microbiology : Journal of Microbiology
© The Microbiological Society of Korea.
TOP