Roles of RpoS in Yersinia pseudotuberculosis stress survival, motility, biofilm formation and type VI secretion system expression

Jingyuan Guan; Xiao Xiao; Shengjuan Xu; Fen Gao; Jianbo Wang; Tietao Wang; Yunhong Song; Junfeng Pan; Xihui Shen; Yao Wang

doi:10.1007/s12275-015-0099-6

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Roles of RpoS in Yersinia pseudotuberculosis stress survival, motility, biofilm formation and type VI secretion system expression: Jingyuan Guan ^1,2, Xiao Xiao ³, Shengjuan Xu ^1,2, Fen Gao ^1,3, Jianbo Wang ^1,2, Tietao Wang ³, Yunhong Song ³, Junfeng Pan ^1,2, Xihui Shen ^1,3, Yao Wang ^1,2; Journal of Microbiology 2015;53(9):633-642.
DOI: https://doi.org/10.1007/s12275-015-0099-6
Published online: August 27, 2015

¹State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China, ²Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China, ³Department of Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China¹State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China, ²Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China, ³Department of Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China

Corresponding author: Xihui Shen , Tel: +86-29-87081062,
Yao Wang , Tel: +86-29-87081062,

Received: 26 February 2015 • Revised: 16 July 2015 • Accepted: 22 July 2015

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Abstract

RpoS (σS), the stationary phase/stress σ factor, controls the expression of a large number of genes involved in cellular responses to a variety of stresses. However, the role of RpoS appears to differ in different bacteria. While RpoS is an important regulator of flagellum biosynthesis, it is associated with biofilm development in Edwardsiella tarda. Biofilms are dense communities formed by bacteria and are important for microbe survival under unfavorable conditions. The type VI secretion system (T6SS) discovered recently is reportedly associated with several phenotypes, ranging from biofilm formation to stress sensing. For example, Vibrio anguillarum T6SS was proposed to serve as a sensor for extracytoplasmic signals and modulates RpoS expression and stress response. In this study, we investigated the physiological roles of RpoS in Yersinia pseudotuberculosis, including bacterial survival under stress conditions, flagella formation, biofilm development and T6SS expression. We found that RpoS is important in resistance to multiple stressors–including H2O2, acid, osmotic and heat shock–in Y. pseudotuberculosis. In addition, our study showed that RpoS not only modulates the expression of T6SS but also regulates flagellum formation by positively controlling the flagellar master regulatory gene flhDC, and affects the formation of biofilm on Caenorhabditis elegans by regulating the synthesis of exopolysaccharides. Taken together, these results show that RpoS plays a central role in cell fitness under several adverse conditions in Y. pseudotuberculosis.

Keywords: Yersinia pseudotuberculosis;RpoS;T6SS;stress resistance;motility;biofilm

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Roles of RpoS in Yersinia pseudotuberculosis stress survival, motility, biofilm formation and type VI secretion system expression

J. Microbiol. 2015;53(9):633-642. Published online August 27, 2015

DOI: https://doi.org/10.1007/s12275-015-0099-6

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