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- Functional Characterization of Autographa californica Multiple Nucleopolyhedrovirus ORF43 and Phenotypic Changes of ORF43-Knockout Mutant
- Xue Ying Tao , Jae Young Choi , Yong Wang , Jong Yul Roh , Joo Hyun Lee , Qin Liu , Jong Bin Park , Jae Su Kim , Woojin Kim , Yeon Ho Je
- J. Microbiol. 2013;51(4):515-521. Published online August 30, 2013
- DOI: https://doi.org/10.1007/s12275-013-3058-0
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Abstract
- ORF43 (ac43) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a highly conserved baculovirus gene of unknown function. To investigate the role of ac43 in the baculovirus lifecycle, we constructed an ac43-deleted mutant AcMNPV, Ac43KO. After transfection into Spodoptera frugiperda cells, Ac43KO produced polyhedra much larger in size than those of wild-type AcMNPV. Interestingly, some of the nucleocapsids were singly enveloped in the polyhedrin matrix while the nucleocapsids of AcMNPV are known to be multiply enveloped. Furthermore, Ac43KO led to a defect in the transcription and expression of polyhedrin, which resulted in reduced occlusion body production. However, Ac43KO did not affect production of budded virus as there was no remarkable difference in budded virus titer. These results suggest that ac43 plays an important role in the expression of polyhedrin, the morphogenesis of occlusion body, and the assembly of virions occluded in occlusion bodies.
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Citations
Citations to this article as recorded by
- Successful Rescue of Synthetic AcMNPV with a ~17 kb Deletion in the C1 Region of the Genome
Yijia Guo, Hengrui Hu, Han Xiao, Fei Deng, Jiang Li, Manli Wang, Zhihong Hu
Viruses.2022; 14(12): 2780. CrossRef - Systematic analysis of nuclear localization of Autographa californica multiple nucleopolyhedrovirus proteins
Lihong He, Wei Shao, Jiang Li, Fei Deng, Hualin Wang, Zhihong Hu, Manli Wang
Journal of General Virology.2021;[Epub] CrossRef - The autographa californica multiple nucleopolyhedrovirus Ac12: A non-essential F box-like protein that interacts with cellular SKP1 component of the E3 ubiquitin ligase complex
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Zhong-Jian Guo, Meng-Han Yu, Xian-Yun Dong, Wei-Li Wang, Ting Tian, Xian-Yin Yu, Xu-Dong Tang
Scientific Reports.2017;[Epub] CrossRef - Disruption of the baculovirus core gene ac78 results in decreased production of multiple nucleocapsid-enveloped occlusion-derived virions and the failure of primary infection in vivo
Sai-Nan Li, Jin-Yu Wang, Mei-Jin Yuan, Kai Yang
Virus Research.2014; 191: 70. CrossRef - The Trichoplusia ni single nucleopolyhedrovirus tn79 gene encodes a functional sulfhydryl oxidase enzyme that is able to support the replication of Autographa californica multiple nucleopolyhedrovirus lacking the sulfhydryl oxidase ac92 gene
Stian A. Clem, Wenbi Wu, A. Lorena Passarelli
Virology.2014; 460-461: 207. CrossRef - Baculovirus nucleocapsid aggregation (MNPV vs SNPV): an evolutionary strategy, or a product of replication conditions?
George F. Rohrmann
Virus Genes.2014; 49(3): 351. CrossRef
- Successful Rescue of Synthetic AcMNPV with a ~17 kb Deletion in the C1 Region of the Genome
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