Functional Characterization of Autographa californica Multiple Nucleopolyhedrovirus ORF43 and Phenotypic Changes of ORF43-Knockout Mutant

Xue Ying Tao; Jae Young Choi; Yong Wang; Jong Yul Roh; Joo Hyun Lee; Qin Liu; Jong Bin Park; Jae Su Kim; Woojin Kim; Yeon Ho Je

doi:10.1007/s12275-013-3058-0

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Research Support, Non-U.S. Gov't
Functional Characterization of Autographa californica Multiple Nucleopolyhedrovirus ORF43 and Phenotypic Changes of ORF43-Knockout Mutant: Xue Ying Tao ¹, Jae Young Choi ², Yong Wang ³, Jong Yul Roh ⁴, Joo Hyun Lee ¹, Qin Liu ¹, Jong Bin Park ², Jae Su Kim ⁵, Woojin Kim ², Yeon Ho Je ^1,2; Journal of Microbiology 2013;51(4):515-521
DOI: https://doi.org/10.1007/s12275-013-3058-0
Published online: August 30, 2013

¹Department of Agricultural Biotechnology, College of Agriculture & Life Science, Seoul National University, Seoul 151-742, Republic of Korea, ²Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea, ³School of Life Science, Lanzhou University, 99 Dong Ganxi Road, Lanzhou 730000, P. R. China, ⁴Division of Medical Entomology, Korea National Institute of Health, Chungbuk 363-951, Republic of Korea, ⁵Department of Agricultural Biology, College of Agricultural Life Science, Chonbuk National University, Jeonju 561-756, Republic of Korea¹Department of Agricultural Biotechnology, College of Agriculture & Life Science, Seoul National University, Seoul 151-742, Republic of Korea, ²Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea, ³School of Life Science, Lanzhou University, 99 Dong Ganxi Road, Lanzhou 730000, P. R. China, ⁴Division of Medical Entomology, Korea National Institute of Health, Chungbuk 363-951, Republic of Korea, ⁵Department of Agricultural Biology, College of Agricultural Life Science, Chonbuk National University, Jeonju 561-756, Republic of Korea

Corresponding author: Yeon Ho Je , Tel: +82-2-880-4706,

Received: 24 January 2013 • Accepted: 1 April 2013

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Abstract

ORF43 (ac43) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a highly conserved baculovirus gene of unknown function. To investigate the role of ac43 in the baculovirus lifecycle, we constructed an ac43-deleted mutant AcMNPV, Ac43KO. After transfection into Spodoptera frugiperda cells, Ac43KO produced polyhedra much larger in size than those of wild-type AcMNPV. Interestingly, some of the nucleocapsids were singly enveloped in the polyhedrin matrix while the nucleocapsids of AcMNPV are known to be multiply enveloped. Furthermore, Ac43KO led to a defect in the transcription and expression of polyhedrin, which resulted in reduced occlusion body production. However, Ac43KO did not affect production of budded virus as there was no remarkable difference in budded virus titer. These results suggest that ac43 plays an important role in the expression of polyhedrin, the morphogenesis of occlusion body, and the assembly of virions occluded in occlusion bodies.

Keywords: baculovirus; nucleopolyhedrovirus; Autographa californica NPV; ac43; virus morphology

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Functional Characterization of Autographa californica Multiple Nucleopolyhedrovirus ORF43 and Phenotypic Changes of ORF43-Knockout Mutant

J. Microbiol. 2013;51(4):515-521. Published online August 30, 2013

DOI: https://doi.org/10.1007/s12275-013-3058-0

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