Journal of Microbiology

Journal Articles

Medium Chain Length Polyhydroxyalkanoate Production by Engineered Pseudomonas gessardii Using Acetate-formate as Carbon Sources: Woo Young Kim, Seung-Jin Kim, Hye-Rin Seo, Yoonyong Yang, Jong Seok Lee, Moonsuk Hur, Byoung-Hee Lee, Jong-Geol Kim, Min-Kyu Oh; J. Microbiol. 2024;62(7):569-579. Published online May 3, 2024; DOI: https://doi.org/10.1007/s12275-024-00136-x

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Production of medium chain length polyhydroxyalkanoate (mcl-PHA) was attempted using Pseudomonas gessardii NIBRBAC000509957, which was isolated from Sunchang, Jeollabuk-do, Republic of Korea (35°24'27.7"N, 127°09'13.0"E) and effectively utilized acetate and formate as carbon sources. We first evaluated the utilization of acetate as a carbon source, revealing optimal growth at 5 g/L acetate. Then, formate was supplied to the acetate minimal medium as a carbon source to enhance cell growth. After overexpressing the acetate and formate assimilation pathway enzymes, this strain grew at a significantly higher rate in the medium. As this strain naturally produces PHA, it was further engineered metabolically to enhance mcl-PHA production. The engineered strain produced 0.40 g/L of mcl-PHA with a biomass content of 30.43% in fed-batch fermentation. Overall, this strain can be further developed to convert acetate and formate into valuable products.

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Formate-driven photoautotrophic growth and biopolymer storage in anaerobic purple bacteria
Mohammad Adib Ghazali Abdul Rahman, Bronwyn Laycock, Steven Pratt, Damien J. Batstone
Bioresource Technology.2025; 434: 132753. CrossRef
Sulphide and oleic acid synergism in accelerating mcl-PHA biopolymer production in Pseudomonas aeruginosa MCC 5300 by modulating electron transport system
Raghavendra Paduvari, Divyashree Somashekara
Biochemistry and Biophysics Reports.2025; 44: 102286. CrossRef
Unlocking efficient polyhydroxyalkanoate production by Gram-positive Priestia megaterium using waste-derived feedstocks
Xinyi Bai, Libo Xu, Kang Li, Guangbao Zhang, Mengjun Zhang, Yi Huang
Microbial Cell Factories.2025;[Epub] CrossRef

Enhancement of the solubility of recombinant proteins by fusion with a short-disordered peptide: Jun Ren , Suhee Hwang , Junhao Shen , Hyeongwoo Kim , Hyunjoo Kim , Jieun Kim , Soyoung Ahn , Min-gyun Kim , Seung Ho Lee , Dokyun Na; J. Microbiol. 2022;60(9):960-967. Published online July 14, 2022; DOI: https://doi.org/10.1007/s12275-022-2122-z

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In protein biotechnology, large soluble fusion partners are widely utilized for increased yield and solubility of recombinant proteins. However, the production of additional large fusion partners poses an additional burden to the host, leading to a decreased protein yield. In this study, we identified two highly disordered short peptides that were able to increase the solubility of an artificially engineered aggregationprone protein, GFP-GFIL4, from 0.6% to 61% (D3-DP00592) and 46% (D4-DP01038) selected from DisProt database. For further confirmation, the peptides were applied to two insoluble E. coli proteins (YagA and YdiU). The peptides also enhanced solubility from 52% to 90% (YagA) and from 27% to 93% (YdiU). Their ability to solubilize recombinant proteins was comparable with strong solubilizing tags, maltosebinding protein (40 kDa) and TrxA (12 kDa), but much smaller (< 7 kDa) in size. For practical application, the two peptides were fused with a restriction enzyme, I-SceI, and they increased I-SceI solubility from 24% up to 75%. The highly disordered peptides did not affect the activity of I-SceI while I-SceI fused with MBP or TrxA displayed no restriction activity. Despite the small size, the highly disordered peptides were able to solubilize recombinant proteins as efficiently as conventional fusion tags and did not interfere with the function of recombinant proteins. Consequently, the identified two highly disordered peptides would have practical utility in protein biotechnology and industry.

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A review on computational models for predicting protein solubility
Teerapat Pimtawong, Jun Ren, Jingyu Lee, Hyang-Mi Lee, Dokyun Na
Journal of Microbiology.2025; 63(1): e:2408001. CrossRef
Synthetic intrinsically disordered protein fusion tags that enhance protein solubility
Nicholas C. Tang, Jonathan C. Su, Yulia Shmidov, Garrett Kelly, Sonal Deshpande, Parul Sirohi, Nikhil Peterson, Ashutosh Chilkoti
Nature Communications.2024;[Epub] CrossRef
Biosynthesis of Indigo Dyes and Their Application in Green Chemical and Visual Biosensing for Heavy Metals
Yan Guo, Shun-Yu Hu, Can Wu, Chao-Xian Gao, Chang-Ye Hui
ACS Omega.2024; 9(31): 33868. CrossRef
Functional small peptides for enhanced protein delivery, solubility, and secretion in microbial biotechnology
Hyang-Mi Lee, Thi Duc Thai, Wonseop Lim, Jun Ren, Dokyun Na
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Directed Evolution of Soluble α-1,2-Fucosyltransferase Using Kanamycin Resistance Protein as a Phenotypic Reporter for Efficient Production of 2'-Fucosyllactose
Jonghyeok Shin, Seungjoo Kim, Wonbeom Park, Kyoung Chan Jin, Sun-Ki Kim, Dae-Hyuk Kweon
Journal of Microbiology and Biotechnology.2022; 32(11): 1471. CrossRef
Effects of spray drying, freeze drying, and vacuum drying on physicochemical and nutritional properties of protein peptide powder from salted duck egg white
Tianyin Du, Jicheng Xu, Shengnan Zhu, Xinjun Yao, Jun Guo, Weiqiao Lv
Frontiers in Nutrition.2022;[Epub] CrossRef

Cr(VI) removal from aqueous solution by thermophilic denitrifying bacterium Chelatococcus daeguensis TAD1 in the presence of single and multiple heavy metals: Han Li , Shaobin Huang , Yongqing Zhang; J. Microbiol. 2016;54(9):602-610. Published online August 31, 2016; DOI: https://doi.org/10.1007/s12275-016-5295-5

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Abstract PDF

Cr(VI) pollution is increasing continuously as a result of ongoing industrialization. In this study, we investigated the thermophilic denitrifying bacterium Chelatococcus daeguensis TAD1, isolated from the biofilm of a biotrickling filter used in nitrogen oxides (NOX) removal, with respect to its ability to remove Cr(VI) from an aqueous solution. TAD1 was capable of reducing Cr(VI) from an initial concentration of 10 mg/L to non-detectable levels over a pH range of 7–9 and at a temperature range of 30–50°C. TAD1 simultaneously removed both Cr(VI) and NO3 −-N at 50°C, when the pH was 7 and the initial Cr(VI) concentration was 15 mg/L. The reduction of Cr(VI) to Cr(III) correlated with the growth metabolic activity of TAD1. The presence of other heavy metals (Cu, Zn, and Ni) inhibited the ability of TAD1 to remove Cr(VI). The metals each individually inhibited Cr(VI) removal, and the extent of inhibition increased in a cooperative manner in the presence of a combination of the metals. The addition of biodegradable cellulose acetate microspheres (an adsorption material) weakened the toxicity of the heavy metals; in their presence, the Cr(VI) removal efficiency returned to a high level. The feasibility and applicability of simultaneous nitrate removal and Cr(VI) reduction by strain TAD1 is promising, and may be an effective biological method for the clean-up of wastewater.

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Hexavalent Chromium Pollution and its Sustainable Management through Bioremediation
Anushka Paul, Sudeshna Dey, Deo Karan Ram, Alok Prasad Das
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Adsorption of Hg2+/Cr6+ by metal-binding proteins heterologously expressed in Escherichia coli
Shuting Hu, Zixiang Wei, Teng Liu, Xinyu Zuo, Xiaoqiang Jia
BMC Biotechnology.2024;[Epub] CrossRef
Growth-dependent cr(VI) reduction by Alteromonas sp. ORB2 under haloalkaline conditions: toxicity, removal mechanism and effect of heavy metals
G. Kiran Kumar Reddy, K. Kavibharathi, Anuroop Singh, Y. V. Nancharaiah
World Journal of Microbiology and Biotechnology.2024;[Epub] CrossRef
Hexavalent Cr, Its Toxicity and Removal Strategy: Revealing PGPB Potential in Its Remediation
Akanksha Gupta, Anubhuti Singh, Virendra Kumar Mishra
Water, Air, & Soil Pollution.2023;[Epub] CrossRef
Chromium Toxicity in Plants: Signaling, Mitigation, and Future Perspectives
Sajad Ali, Rakeeb A. Mir, Anshika Tyagi, Nazia Manzar, Abhijeet Shankar Kashyap, Muntazir Mushtaq, Aamir Raina, Suvin Park, Sandhya Sharma, Zahoor A. Mir, Showkat A. Lone, Ajaz A. Bhat, Uqab Baba, Henda Mahmoudi, Hanhong Bae
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A comprehensive review on chromium (Cr) contamination and Cr(VI)-resistant extremophiles in diverse extreme environments
Zeeshanur Rahman, Lebin Thomas, Siva P. K. Chetri, Shrey Bodhankar, Vikas Kumar, Ravi Naidu
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Health hazards of hexavalent chromium (Cr (VI)) and its microbial reduction
Pooja Sharma, Surendra Pratap Singh, Sheetal Kishor Parakh, Yen Wah Tong
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Xinyang Liu, Wanting Liu, Zifang Chi
Journal of Water Process Engineering.2022; 49: 103188. CrossRef
Simultaneous denitrification and hexavalent chromium removal by a newly isolated Stenotrophomonas maltophilia strain W26 under aerobic conditions
Qiang An, Shu-man Deng, Bin Zhao, Zheng Li, Jia Xu, Jia-Li Song
Environmental Chemistry.2021; 18(1): 20. CrossRef
Leucobacter coleopterorum sp. nov., Leucobacter insecticola sp. nov., and Leucobacter viscericola sp. nov., isolated from the intestine of the diving beetles, Cybister brevis and Cybister lewisianus, and emended description of the genus Leucobacter
Dong-Wook Hyun, Hojun Sung, Pil Soo Kim, Ji-Hyun Yun, Jin-Woo Bae
Journal of Microbiology.2021; 59(4): 360. CrossRef
Bioremediation of Chromium by Microorganisms and Its Mechanisms Related to Functional Groups
Abate Ayele, Yakob Godebo Godeto, Yifeng Zhang
Journal of Chemistry.2021; 2021: 1. CrossRef
Iron oxide minerals promote simultaneous bio-reduction of Cr(VI) and nitrate: Implications for understanding natural attenuation
Yutian Hu, Tong Liu, Nan Chen, Chuanping Feng
Science of The Total Environment.2021; 786: 147396. CrossRef
Cr(VI) reductase activity locates in the cytoplasm of Aeribacillus pallidus BK1, a novel Cr(VI)-reducing thermophile isolated from Tengchong geothermal region, China
Yan Ma, Hui Zhong, Zhiguo He
Chemical Engineering Journal.2019; 371: 524. CrossRef

High Efficiency Transformation by Electroporation of Yarrowia lipolytica: Jia-Hung Wang , Wenpin Hung , Shu-Hsien Tsai; J. Microbiol. 2011;49(3):469-472. Published online June 30, 2011; DOI: https://doi.org/10.1007/s12275-011-0433-6

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Abstract PDF

Yarrowia lipolytica was usually transformed by heat shock, but linearized integrative vectors always resulted in a low transformation efficiency when electroporation was used. To develop a high efficiency integrative transformation method by electroporation of Y. lipolytica, we report here that pretreatment of Y. lipolytica with 150 mM LiAc for 1 h before electroporation will approximately 30-fold of increase transformation efficiency. A cell concentration of 1010/ml and instrument settings of 1.5 kV will generate the highest transformation efficiencies. We have developed a procedure to transform Y. lipolytica that will be able to yield an efficiency of 2.1×104 transformants/μg for integrative linear DNA. With our modifications, the electroporation procedures became a very efficient and reliable tool for Y. lipolytica transformation.

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Biotechnology Advances.2022; 59: 107984. CrossRef
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Catherine Madzak
Journal of Fungi.2021; 7(7): 548. CrossRef
Revisiting the unique structure of autonomously replicating sequences in Yarrowia lipolytica and its role in pathway engineering
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Caleb Walker, Seunghyun Ryu, Richard J. Giannone, Sergio Garcia, Cong T. Trinh, Haruyuki Atomi
Applied and Environmental Microbiology.2020;[Epub] CrossRef
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Aiqun Yu, Yu Zhao, Jian Li, Shenglong Li, Yaru Pang, Yakun Zhao, Cuiying Zhang, Dongguang Xiao
MicrobiologyOpen.2020;[Epub] CrossRef
Secretion of a low and high molecular weight β-glycosidase by Yarrowia lipolytica
Paul Swietalski, Frank Hetzel, Ines Seitl, Lutz Fischer
Microbial Cell Factories.2020;[Epub] CrossRef
A novel electroporation procedure for highly efficient transformation of Lipomyces starkeyi
Hiroaki Takaku, Atsumi Miyajima, Haruka Kazama, Rikako Sato, Satoshi Ara, Tomohiko Matsuzawa, Katsuro Yaoi, Hideo Araki, Yosuke Shida, Wataru Ogasawara, Harutake Yamazaki
Journal of Microbiological Methods.2020; 169: 105816. CrossRef
Homology‐independent genome integration enables rapid library construction for enzyme expression and pathway optimization in Yarrowia lipolytica
Zhiyong Cui, Xin Jiang, Huihui Zheng, Qingsheng Qi, Jin Hou
Biotechnology and Bioengineering.2019; 116(2): 354. CrossRef
Electroporation of germinated conidia and young mycelium as an efficient transformation system for Acremonium chrysogenum
Jessica Cruz-Ramón, Francisco J. Fernández, Armando Mejía, Francisco Fierro
Folia Microbiologica.2019; 64(1): 33. CrossRef
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Seunghyun Ryu, Cong T. Trinh, Marie A. Elliot
Applied and Environmental Microbiology.2018;[Epub] CrossRef
Metabolic engineering in the host Yarrowia lipolytica
Ahmad M. Abdel-Mawgoud, Kelly A. Markham, Claire M. Palmer, Nian Liu, Gregory Stephanopoulos, Hal S. Alper
Metabolic Engineering.2018; 50: 192. CrossRef
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Jun Ren, Dokyun Na, Seung Min Yoo
Journal of Biotechnology.2018; 288: 55. CrossRef
Recent Advances in Metabolic Engineering of Yarrowia lipolytica for Lipid Overproduction
Si‐Yu Zeng, Hu‐Hu Liu, Tian‐Qiong Shi, Ping Song, Lu‐Jing Ren, He Huang, Xiao‐Jun Ji
European Journal of Lipid Science and Technology.2018;[Epub] CrossRef
High-efficiency transformation of Yarrowia lipolytica using electroporation
Kelly A Markham, Sofia Vazquez, Hal S Alper
FEMS Yeast Research.2018;[Epub] CrossRef
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Meng Wang, Valerie A. Kickhoefer, Leonard H. Rome, Oliver K. Foellmer, Shaily Mahendra
Biotechnology and Bioengineering.2018; 115(12): 2941. CrossRef
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Hongdi Liu, Xiang Jiao, Yanan Wang, Xiaobing Yang, Wenyi Sun, Jihui Wang, Sufang Zhang, Zongbao Kent Zhao
FEMS Yeast Research.2017;[Epub] CrossRef
New recombinant strains of the yeast Yarrowia lipolytica with overexpression of the aconitate hydratase gene for the obtainment of isocitric acid from rapeseed oil
I. A. Laptev, N. A. Filimonova, R. K. Allayarov, S. V. Kamzolova, V. A. Samoilenko, S. P. Sineoky, I. G. Morgunov
Applied Biochemistry and Microbiology.2016; 52(7): 699. CrossRef
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Yanan Wang, Sufang Zhang, Markus Pötter, Wenyi Sun, Li Li, Xiaobing Yang, Xiang Jiao, Zongbao K. Zhao
Applied Biochemistry and Biotechnology.2016; 180(8): 1497. CrossRef
Recent advances in bioengineering of the oleaginous yeast Yarrowia lipolytica
Murtaza Shabbir Hussain, Gabriel M Rodriguez, Difeng Gao, Michael Spagnuolo, Lauren Gambill, Mark Blenner
AIMS Bioengineering.2016; 3(4): 493. CrossRef
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Huaiyuan Zhang, Chao Wu, Qingyu Wu, Junbiao Dai, Yuanda Song, Wei Ning Chen
PLOS ONE.2016; 11(7): e0159187. CrossRef
Comparison of Nitrogen Depletion and Repletion on Lipid Production in Yeast and Fungal Species
Shihui Yang, Wei Wang, Hui Wei, Stefanie Van Wychen, Philip Pienkos, Min Zhang, Michael Himmel
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Shuyan Zhang, Jeffrey M. Skerker, Charles D. Rutter, Matthew J. Maurer, Adam P. Arkin, Christopher V. Rao
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Applied Microbiology and Biotechnology.2015; 99(10): 4237. CrossRef
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Yang Li, Mengxi Wu, Deyao Zhao, Zewen Wei, Wenfeng Zhong, Xiaoxia Wang, Zicai Liang, Zhihong Li
Scientific Reports.2015;[Epub] CrossRef
Improved electroporation procedure for genetic transformation ofDekkera/Brettanomyces bruxellensis
Marina Miklenić, Bojan Žunar, Anamarija Štafa, Ivan-Krešimir Svetec, Jens Nielsen
FEMS Yeast Research.2015; 15(8): fov096. CrossRef
Biotechnological applications of Yarrowia lipolytica: Past, present and future
Hu-Hu Liu, Xiao-Jun Ji, He Huang
Biotechnology Advances.2015; 33(8): 1522. CrossRef
The Strictly Aerobic Yeast Yarrowia lipolytica Tolerates Loss of a Mitochondrial DNA-Packaging Protein
Jana Bakkaiova, Kosuke Arata, Miki Matsunobu, Bungo Ono, Tomoyo Aoki, Dana Lajdova, Martina Nebohacova, Jozef Nosek, Isamu Miyakawa, Lubomir Tomaska
Eukaryotic Cell.2014; 13(9): 1143. CrossRef
Yarrowia lipolytica as an Oleaginous Cell Factory Platform for Production of Fatty Acid-Based Biofuel and Bioproducts
Ali Abghari, Shulin Chen
Frontiers in Energy Research.2014;[Epub] CrossRef
Regulatory properties of malic enzyme in the oleaginous yeast, Yarrowia lipolytica, and its non-involvement in lipid accumulation
Huaiyuan Zhang, Luning Zhang, Haiqin Chen, Yong Q. Chen, Colin Ratledge, Yuanda Song, Wei Chen
Biotechnology Letters.2013; 35(12): 2091. CrossRef

Research Support, Non-U.S. Gov't

The Schizosaccharomyces pombe Gene Encoding [gamma]-Glutamyl Transpeptidase I Is Regulated by Non-fermentable Carbon Sources and Nitrogen Starvation: Hong-Gyum Kim , Hey-Jung Park , Hyun-Jung Kang , Hye-Won Lim , Kyunghoon Kim , Eun-Hee Park , Kisup Ahn , Chang-Jin Lim; J. Microbiol. 2005;43(1):44-48.; DOI: https://doi.org/2139 [pii]

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Abstract PDF: In our previous study, the first structural gene (GGTI) encoding g-glutamyl transpeptidase was cloned and characterized from the fission yeast Schizosaccharomyces pombe, and its transcription, using the GGTI-lacZ fusion gene, containing the 1,085 bp upstream region from the translational initiation point, was found to be enhanced by sodium nitroprusside and L-buthionine-(S,R)-sulfoximine (BSO). In the present work, regulation of the GGTI gene was further elucidated. Non-fermentable carbon sources, such as acetate and ethanol, markedly enhanced the synthesis of [beta]-galactosidase from the GGTI-lacZ fusion gene. However, its induction by non-fermentable carbon sources appeared to be independent of the presence of the Pap1 protein. Nitrogen starvation also gave rise to induction of GGTI gene expression in a Pap1-independent manner. The three additional fusion plasmids, carrying 754, 421 and 156 bp regions, were constructed. The sequence responsible for the induction by non-fermentable carbon sources and nitrogen starvation was identified to exist within a -421 bp region of the GGTI gene. Taken together, the S. pombe GGTI gene is regulated by non-fermentable carbon sources and nitrogen starvation.

Published Erratum

Erratum to: Introducing EzAAI: A Pipeline for High Throughput Calculations of Prokaryotic Average Amino Acid Identity: Dongwook Kim , Sein Park , Jongsik Chun; J. Microbiol. 2023;61(9):879-879.; DOI: https://doi.org/10.1007/s12275-023-00075-z

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